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Biotechnology Progress

Different types of aqueous two-phase systems for biomolecule and bioparticle extraction and purification

Authors

  • João Vitor Dutra Molino,

    1. Dept. of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 580, Block 16, Cidade Universitária, São Paulo, Brazil
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  • Daniela de Araújo Viana Marques,

    1. Dept. of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 580, Block 16, Cidade Universitária, São Paulo, Brazil
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  • Adalberto Pessoa Júnior,

    Corresponding author
    1. Dept. of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 580, Block 16, Cidade Universitária, São Paulo, Brazil
    • Correspondence concerning this article should be addressed to A. Pessoa Jr at pessoajr@usp.br.

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  • Priscila Gava Mazzola,

    1. Dept. of Clinical Patology, Faculty of Medical Sciences, University of Campinas, Rua: Tessália Vieira de Camargo, 126, Cidade Universitária "Zeferino Vaz, Campinas, São Paulo, Brazil
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  • Maria Silvia Viccari Gatti

    1. Genetics, Evolution and Bioagents Dept., Biology Institute, University of Campinas, Rua: Monteiro Lobato, 255, Cidade Universitária “Zeferino Vaz,” Campinas, São Paulo, Brazil
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Abstract

Upstream improvements have led to significant advances in the productivity of biomolecules and bioparticles. Today, downstream processes are the bottleneck in the production of some biopharmaceuticals, a change from previous years. Current purification platforms will reach their physical limits at some point, indicating the need for new approaches. This article reviews an alternative method to extract and purify biomolecules/bioparticles named aqueous two-phase system (ATPS). Biocompatibility and readiness to scale up are some of the ATPS characteristics. We also discuss some of ATPS applications in the biotechnology field. © 2013 American Institute of Chemical Engineers Biotechnol. Prog., 29:1343–1353, 2013

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