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ChemPhysChem

Single-Molecule DNA Force Spectroscopy to Probe Interactions with the Tri-Peptide Lys-Trp-Lys

Authors

  • Dr. Chandrashekhar U. Murade,

    1. Physics of Complex Fluids Group, University of Twente (The Netherlands)
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  • Prof. Dr. Vinod Subramaniam,

    1. Nanobiophysics Group, Faculty of Science and Technology and MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE, Enschede (The Netherlands), Fax: (+31) 53-489-1105
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  • Dr. Cees Otto,

    Corresponding author
    1. Medical Cell Biophysics, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente (The Netherlands), Fax: (+31) 53-489-3511
    • Medical Cell Biophysics, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente (The Netherlands), Fax: (+31) 53-489-3511
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  • Dr. Martin L. Bennink

    Corresponding author
    1. Nanobiophysics Group, Faculty of Science and Technology and MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE, Enschede (The Netherlands), Fax: (+31) 53-489-1105
    • Nanobiophysics Group, Faculty of Science and Technology and MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE, Enschede (The Netherlands), Fax: (+31) 53-489-1105
    Search for more papers by this author

Abstract

May the force be with you: It is shown that single-molecule optical tweezers force spectroscopy can be used successfully to detect the effects of binding of low-affinity ligands to a single DNA molecule (see picture) and to determine various kinetic parameters of the reaction at single-molecular level.

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