Corresponding author J.-H. Wang: Department of Molecular Biosciences, University of Kansas, Lawrence, KS 66045, USA. Email: firstname.lastname@example.org
The role of Ca2+-calmodulin (CaM) signalling cascades in modulating glutamatergic synaptic transmission on CA1 non-pyramidal fast-spiking neurons was investigated using whole-cell recording and perfusion in rat hippocampal slices.
Paired stimuli (PS), consisting of postsynaptic depolarization to 0 mV and presynaptic stimulation at 1 Hz for 30 s, enhanced excitatory postsynaptic currents (EPSCs) on non-pyramidal neurons in the stratum pyramidale (SP). The potentiation was reduced by the extracellular application of d-amino-5-phosphonovaleric acid (DAP-5, 40 μm), and blocked by the postsynaptic perfusion of 1,2-bis(2-aminophenoxy)-ethane-N,N,N′,N′-tetraacetic acid (BAPTA, 10 mm), a CaM-binding peptide (100 μm) or CaMKII (281–301) (an autoinhibitory peptide of CaM-dependent protein kinases, 100 μm).
The application of adenophostin, an agonist of inositol trisphosphate receptors (IP3Rs) that evokes Ca2+ release, into SP non-pyramidal neurons via the patch pipette (1 μm) enhanced EPSCs and occluded PS-induced synaptic potentiation. The co-application of BAPTA (10 mm) with adenophostin blocked synaptic potentiation. In addition, Ca2+-CaM (40:10 μm) induced synaptic potentiation, which occluded PS-induced potentiation and was attenuated by introducing CaMKII (281–301) (100 μm). EPSCs were sensitive to an antagonist of α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor (AMPAR).
Application of Ca2+-CaM into SP non-pyramidal neurons induced the emergence of AMPAR-mediated EPSCs that were not evoked by low stimulus intensity before perfusion. Ca2+-CaM also increased the amplitude and frequency of spontaneous EPSCs. A scavenger of nitric oxide, carboxy-PTIO (30 μm in slice-perfusion solution), did not affect these increases in sEPSCs.
The magnitude of PS-, adenophostin- or Ca2+-CaM-induced synaptic potentiation in SP non-pyramidal neurons increased during postnatal development.
These results indicate that Ca2+-CaM signalling pathways in CA1 SP non-pyramidal neurons up-regulate glutamatergic synaptic transmission probably through the conversion of inactive-to-active synapses.
If you can't find a tool you're looking for, please click the link at the top of the page to go "Back to old version". We'll be adding more features regularly and your feedback is important to us, so please let us know if you have comments or ideas for improvement.
Interneurons in the central nervous system integrate feedforward and feedback inputs, and control the excitation and synchronous activity of the principal neurons (Wong et al. 1986; Lacaille et al. 1987; Alger, 1991; Freund & Buzsaki, 1996). The up-regulation of glutamatergic synapses on an interneuron increases its activity, enabling the interneuron to limit principal neuron hyperactivity more effectively. Several publications indicate that excitatory synapses on hippocampal interneurons express tetanus-induced long-term potentiation (Buzsaki & Eidelberg, 1982; Taube & Schwartzkroin, 1987; Ouardouz & Lacaille, 1995; Maccaferri & McBain, 1996). However, little is known about how intracellular signalling cascades modulate synaptic plasticity in interneurons.
The activation of glutamate receptors raised Ca2+ levels in hippocampal CA1 interneurons (Carmant et al. 1997). Synaptic potentiation in these interneurons required an increase in intracellular Ca2+ (Ouardouz & Lacaille, 1995). We are interested in determining whether the increase of intracellular Ca2+-CaM and the activity of glutamatergic synapses are inter-dependent. The postsynaptic perfusions of an IP3R agonist or Ca2+-CaM (Wang & Kelly, 1995) and the paired stimuli (Kelso et al. 1986; Maccaferri & McBain, 1996) were used to activate Ca2+-CaM signalling cascades in hippocampal CA1 non-pyramidal neurons. Paired stimuli consisted of postsynaptic depolarization to 0 mV and presynaptic stimulation at 1 Hz for 30 s. The use of these protocols should shed light on investigating monosynaptic plasticity, since tetanic stimulation increased the probability of firing action potentials in pyramidal neurons (Andersen et al. 1980), thereby activating more synapses of recurrent axons onto interneurons (Maccaferri & McBain, 1996).
Axon arbors of CA1 interneurons in the stratum pyramidale (SP) mainly synapse on the soma and proximal dendrites of pyramidal neurons (Freund & Buzsaki, 1996). This subcellular architecture enables SP-interneurons to inhibit pyramidal neurons more efficiently. In view of this functional importance, we have studied intracellular signalling mechanisms of synaptic plasticity in CA1 SP non-pyramidal neurons. Our results indicate that excitatory synapses on these neurons express monosynaptic potentiation, in which the postsynaptic Ca2+-CaM signalling pathways and the conversion of inactive-to-active synapses are involved. These mechanisms are enhanced during postnatal development.
Hippocampal slices and solution
Slices (400 μm) were prepared from Sprague-Dawley rats in postnatal days (PND) 7-22. Rats were anaesthetized by the inhalation of methoxyflurane (2 ml in a 4 l bell-jar) and then decapitated by a guillotine. Tissue blocks including the hippocampus and partial cortex were quickly isolated in oxygenated (95 % O2 and 5 % CO2) ice-cold artificial cerebrospinal fluid (ACSF), in which 0.5 mm CaCl2 and 4 mm MgSO4 were used to reduce excitation. Slices were cut with a Vibratome, and then held in oxygenated standard ACSF (mm): 124 NaCl, 3 KCl, 1.2 NaH2PO4, 2.4 CaCl2, 1.3 MgSO4, 10 dextrose, and 10 Hepes at 25 °C for 1-2 h. A slice was transferred to a submersion chamber (Warner RC-26G) and perfused with oxygenated standard ACSF at 31 °C for electrophysiological recordings. The concentration of KCl was raised to 4.5 mm to increase the basal level of spontaneous synaptic activity in studying the effect of Ca2+-CaM on sEPSCs (spontaneous excitatory postsynaptic currents).
Bipolar tungsten electrodes (12 MΩ) were used to stimulate Schaffer collateral and/or commissural (S/C) fibres in area CA1. They were located away from the recording neurons to prevent direct triggering of long and/or irregular arbors of interneurons and to reduce the possibility of evoking polysynaptic activity. Stimulus frequency was 0.1 Hz. Paired stimuli for inducing synaptic potentiation were postsynaptic depolarization to 0 mV and 1 Hz presynaptic stimulation for 30 s. Stimulus intensity for studying inactive synapses was set just below the values to evoke EPSCs at the first stimulus in paired pulses when the standard solution was in the pipette tip.
Recording neurons in the hippocampal area CA1 were initially selected based on their morphology under DIC microscope (Nikon E600FN or Olympus BX50) and electrophysiological properties. Compared with pyramidal neurons, the selected neurons appeared small (10-15 μm) with round or irregular soma and multipolar processes, i.e. non-pyramidal. The membrane of these non-pyramidal neurons displayed higher input resistances and smaller decay-time constants in response to hyperpolarization pulses. Depolarization pulses (60 ms) induced high frequency discharges (fast spiking), in which action potentials appeared as short-duration, deep fast after-hyperpolarization with little frequency and amplitude accommodation (see waveforms in Fig. 1D). These properties match the criteria of interneurons (Lacaille et al. 1987; Freund & Buzsaki, 1996; McBain et al. 1999). Some recording neurons in each experimental group were labelled by perfusing neurobiotin for further identification (Figs 1, 4-6 and 9-10), and none of them appeared pyramidal-like. Neurons in our studies were non-pyramidal and fast spiking.
EPSCs (evoked or spontaneous) were recorded in non-pyramidal neurons by whole-cell voltage clamp (Axopatch-1D amplifier, Axon Instrument Inc.) with holding potentials between -70 and -72 mV. At the end of experiments, some slices were perfused with 10 μm 6-cyano-7-nitroquinoxaline-2,3-(1H,4H)-dione (CNQX) to examine whether EPSCs were mediated by the AMPA-type glutamate receptors (Fig. 4-6A and Fig. 11). Standard pipette solution (control) contained (mm): 135 potassium gluconate, 15 KCl, 5 NaCl, 10 Hepes, 0.5 EGTA, 4 Mg-ATP and 0.5 Tris-GTP, and was filtered with a centrifuge filter (0.1 μm pores). Care was taken to maintain the osmolarity of pipette solution as 295-310 mosmol l−1. The resistance of the whole-cell pipettes was 6-8 MΩ.
Recording pipettes were also used to perfuse modulators of signalling pathways into non-pyramidal neurons (postsynaptic neurons) to examine their effects on glutamatergic synaptic transmission. The stock solution of modulators was prepared with distilled water (100 times higher than the final concentration), and diluted with the standard pipette solution before use. Ca2+-CaM was made by mixing Ca2+ and CaM at a molar ratio of 4:1 (Wang & Kelly, 1995). The concentration of inhibitors was above their IC50 but this did not appear to affect basal synaptic transmission under control conditions, and thus the effects of the inhibitors on synaptic potentiation during co-perfusion experiments were probably not due to their side-effects. Whole-cell perfusion was done by filling pipette tips with standard solution and the back-filled pipette with modulator-containing solution, which allows comparisons of the effect of modulators on synaptic transmission with control. The success of this method has been examined by perfusing neurobiotin and examining the resultant fluorescence in the recording neurons 2-4 min after the formation of the whole-cell configuration.
Data were collected and analysed only if neurons displayed stable membrane potentials in the range -65 to -70 mV with no significant changes in series and input resistances throughout each experiment. Series and input resistances were monitored in all experiments by measuring responses to voltage pulses of 5 mV and 50 ms (see the first part of representative waveforms in the figures). We started recording EPSCs when whole-cell access was established and where instantaneous currents were at least threefold higher than steady-state currents (Marty & Neher, 1995). We measured the amplitudes of population, emerged and spontaneous EPSCs. The first population of EPSC was defined as baseline (100 %) and used to normalize the remaining monosynaptic EPSCs. As adenophostin- or Ca2+-CaM-induced synaptic potentiation developed quickly (see Results), the use of the first EPSC as the baseline should minimize the effects of modulators on baseline values. Spontaneous EPSCs were accounted for only when the ratio of sEPSC to baseline noise was above three. Synaptic strengths are presented as means ± standard error of the mean. Values for comparison among groups were selected at the same time (30 min) and tested by ANOVA. Representative waveforms of population EPSCs were averaged from four consecutive responses. Those of emerged and sEPSCs were superimposed from 10 and 6 traces, respectively.
Morphology of recording neurons
Neurobiotin was dissolved in pipette solutions at a final concentration of 0.25-1 %, and back-filled into recording pipettes whose tips contained the standard solution. After recordings, hippocampal slices were perfused with oxygenated ACSF for an additional 30 min to allow neurobiotin filling of fine processes. Then, slices were fixed with 4 % paraformaldehyde, 0.25 % glutaldehyde and 1 % picric acid in 0.1 m phosphate buffer (PBS) at 4 °C for 24-72 h. Slices were incubated in avidin and/or horseradish peroxidase (Vectastain ABC) for 3 h, and in 1 % DAB-CoCl2 (Sigma) 1 min for staining neurobiotin-filled neurons. The reaction was stopped by PBS. Neurobiotin-stained neurons were photographed through an inverted microscope (Nikon Diaphot, × 20 and × 40 objectives).
1,2-bis(2-Aminophenoxy)-ethane-N,N,N′,N′-tetraacetic acid (BAPTA), d-amino-5-phosphonovaleric acid (DAP-5), CNQX and carboxy-PTIO (2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide) were purchased from Sigma. Calmodulin (CaM), CaM-binding peptide and CaMKII (281-301) were from Calbiochem. Other chemicals were from Fisher Scientific. Adenophostin was a gift from Dr Masaaki Takahashi (Biological Research Laboratory, Sankyo Co., Ltd, Japan).
Paired stimuli induce synaptic potentiation on CA1 SP non-pyramidal neurons
We studied synaptic plasticity on non-pyramidal neurons in CA1 stratum pyramidale (SP) using paired stimuli (PS, see Methods), which presumably prevents tetanus-induced polysynaptic plasticity in interneurons (Maccaferri & McBain, 1996). Recording pipettes contained the standard solution, and some were back-filled with neurobiotin for labelling neurons. We did not observe significant changes in excitatory postsynaptic currents (EPSCs) in 40 min (98 ± 3 %, n = 6; open symbols in Fig. 1a). In another group with the same age (postnatal days 18-22), PS was applied after 8 min of whole-cell recording. The amplitude of EPSCs gradually increased, and the average level of potentiation at 30 min was 199 ± 15 % (n = 8; filled symbols in Fig. 1a). These results indicate that PS induces synaptic potentiation in CA1 SP non-pyramidal neurons.
In addition to selecting neurons based on their non-pyramidal shape as viewed under a DIC microscope and fast-spiking in response to depolarization (Fig. 1D), we labelled some of them with neurobiotin. None of these stained neurons appears pyramidal-like, and Fig. 1C shows two examples. Hippocampal CA1 SP-neurons in our studies are non-pyramidal and fast spiking, i.e. interneurons (Freund & Buzsaki, 1996).
PS-induced synaptic potentiation requires a Ca2+-CaM signalling pathway
The PS protocol may activate N-methyl-d-aspartate receptors (NMDARs; Mayer et al. 1989; Jahr & Stevens, 1990) and voltage-gated calcium channels (Fox et al. 1987; Tsien et al. 1988). Ca2+ inflow may evoke Ca2+ release (Ehrlich, 1995; Berridge, 1998). To examine an involvement of postsynaptic Ca2+ increases in PS-induced synaptic potentiation, we preloaded BAPTA (10 mm in pipettes), a quick chelator of Ca2+ (Tsien, 1980), into SP non-pyramidal neurons (PND 18-22). The PS failed to induce synaptic potentiation (99 ± 6 %, n = 7, filled symbols in Fig. 2a) compared to control with the standard pipette solution (199 ± 15 %, n = 8, open symbols). These results indicate that postsynaptic Ca2+ increases are required for PS-induced synaptic potentiation in SP non-pyramidal neurons.
We have examined the role of NMDARs in PS-induced synaptic potentiation. An NMDAR antagonist, DAP-5 (40 μm), was perfused to hippocampal slices immediately after getting whole-cell recordings of EPSCs in SP non-pyramidal neurons (PND 18-22). After adding DAP-5 for 10 min, PS still enhanced the amplitude of EPSCs (142 ± 8 %, n = 5; filled symbols in Fig. 2B), but to a lesser extent than PS-induced synaptic potentiation under control conditions (199 ± 15 %, n = 8; open symbols; P = 0.006). It is noteworthy that 40 μm DAP-5 blocked EPSCs recorded at +50 mV in 10 μm CNQX (inset b2 of Fig. 2B). These results indicate that the Ca2+ requirement for PS-induced synaptic potentiation in SP non-pyramidal neurons is partially due to the activation of NMDARs. We did not examine the contribution of voltage-gated calcium channels because blocking these channels affected synaptic transmission (data not shown).
As Ca2+ binds CaM with a high affinity (Cohen, 1988; Klee & Cohen, 1988), we examined whether Ca2+-CaM is required for synaptic potentiation. A CaM-binding peptide (CBP, 100 μm in pipettes), an antagonist of Ca2+-CaM (Ocorr & Schulman, 1991; Hanson et al. 1994), was pre-loaded into SP non-pyramidal neurons (PND 18-22). CBP prevented PS-induced synaptic potentiation (96 ± 5 %, n = 5, filled symbols in Fig. 3a), indicating the requirement of Ca2+-CaM. Moreover, we examined whether CaM-dependent protein kinases are involved in PS-induced synaptic potentiation since CaMKII, a target of Ca2+-CaM, is enriched in postsynaptic densities (Kelly et al. 1984; Cohen, 1988; Klee & Cohen, 1988). The pre-loading of an autoinhibitory peptide of these kinases, CaMKII (281-301) (Hanson & Schulman, 1992; 100 μm in pipettes), into postsynaptic neurons attenuated PS-induced synaptic potentiation (109 ± 11 %, n = 5; filled symbols in Fig. 3B). These results indicate that a postsynaptic Ca2+-CaM signalling pathway is involved in PS-induced synaptic potentiation in SP non-pyramidal neurons.
Raising postsynaptic Ca2+ or CaM increases EPSCs on CA1 SP non-pyramidal neurons
If postsynaptic Ca2+-CaM is essential to synaptic potentiation, raising Ca2+ or Ca2+-CaM should enhance synaptic strength. We increased intracellular Ca2+ by perfusing adenophostin (a potent agonist of IP3Rs that evokes Ca2+ release; Takahashi et al. 1994; Delisle et al. 1997) into non-pyramidal neurons (PND 18-22). Compared with control (open symbols), adenophostin (1 μm in pipettes) increased the amplitude of EPSCs (filled symbols in Fig. 4a) that were subsequently blocked by 10 μm CNQX, i.e. glutamatergic. Mean data of adenophostin-induced synaptic potentiation are shown in Fig. 4B (373 ± 31 %, n = 9, open symbols). Similarly, the postsynaptic perfusion of thapsigargin, which increases cytosolic Ca2+ accumulation by inhibiting Ca2+-ATPase (Treiman et al. 1998), enhanced EPSCs (n = 3, data not shown). However, co-perfusing adenophostin with BAPTA (10 mm) failed to induce synaptic potentiation (104 ± 10 %, n = 7, filled symbols in Fig. 4B). These results indicate that postsynaptic Ca2+ enhances synaptic function in non-pyramidal neurons. It is noteworthy that PS could not enhance EPSC amplitudes after adenophostin-induced potentiation (n = 7; an example in Fig. 4a), indicating that PS- and adenophostin-induced synaptic potentiation share similar mechanisms.
Next, we raised Ca2+-CaM in non-pyramidal neurons (PND 18-22) by whole-cell perfusion. Compared with control (open symbols), Ca2+-CaM (40:10 μm in pipettes) increased the amplitude of EPSCs (filled symbols in Fig. 5a) that were subsequently blocked by 10 μm CNQX. Mean data of Ca2+-CaM-induced synaptic potentiation are shown in Fig. 5B (open symbols; 277 ± 25 %, n = 11). Ca2+-CaM-induced synaptic potentiation occluded the PS effect (n = 4, an example in Fig. 5a), indicating that they share similar mechanisms. To examine if Ca2+-CaM activates CaM-dependent protein kinases to enhance EPSCs, we co-perfused CaMKII (281-301) (100 μm in pipettes), which has no CaM-binding sequence (Kelly, 1992), with Ca2+-CaM into non-pyramidal neurons. This co-perfusion blocked Ca2+-CaM-induced synaptic potentiation (106 ± 6 %, n = 13, filled symbols in Fig. 5B). The perfusion of 100 μm CaMKII (281-301) alone did not affect basal synaptic transmission (n = 3, grey symbols in Fig. 5B). These results indicate that CaM-dependent protein kinases involve Ca2+-CaM-induced synaptic potentiation.
The results above indicate that Ca2+-CaM signalling cascades enhance monosynaptic function in non-pyramidal neurons in hippocampal CA1 stratum pyramidale. Increases in EPSCs recorded from a population of synapses may be caused by the conversion of some inactive synapses to active ones, for which signalling molecules may enhance receptor function and/or transmitter release. CaMKII has been shown to phosphorylate glutamate receptors during long-term potentiation (Barria et al. 1997), so we examined whether Ca2+-CaM signalling cascades modulate synaptic targets to convert inactive synapses to active ones.
Ca2+-CaM converts inactive to active synapses on CA1 SP non-pyramidal neurons
If synapses display an extremely low probability of responses to single stimuli, we define them as inactive synapses, which presumably can be activated by multipulse stimuli (facilitation, Zucker, 1989) or signalling cascades. To study the inactive synapse, we need a stimulus protocol that evokes EPSCs at low probability and facilitates them. Paired-pulse stimuli (Wang & Kelly, 1996) were used. Here the stimulus intensity did not evoke EPSCs in pulse one but evoked low amplitude EPSCs in pulse two when the standard solution was in the pipette tip. After a signalling molecule is perfused into the recording neurons, the emergence of EPSCs in the first pulse indicates that this molecule converts inactive synapses to active ones. To perfuse Ca2+-CaM, we filled the pipette tip with standard solution and back-filled with Ca2+-CaM (40:10 μm).
After Ca2+-CaM diffused into non-pyramidal neurons (PND 18-22), the first stimuli evoked EPSCs that were subsequently blocked by 10 μm CNQX (Fig. 6a). The distribution of EPSC amplitudes (same data as Fig. 6a) recorded in 5-30 min (open bars) and 0-4 min (filled bars) is plotted in Fig. 6B. The probability of detecting evoked EPSCs in Fig. 6C was calculated from events in 0-4 and 20-24 min, and the mean values (filled circles) were 0.15 ± 0.02 vs. 0.87 ± 0.04, respectively (n = 12, P < 0.001). When pipettes were back-filled with the standard solution, the mean values of probability in 0-4 and 20-24 min were 0.09 ± 0.01 vs. 0.1 ± 0.03, respectively (n = 6, P = 0.61; filled diamonds in Fig. 6C). These results indicate that the emergence of glutamatergic EPSCs in the first stimulus was due to postsynaptic Ca2+-CaM application, i.e. Ca2+-CaM in non-pyramidal neurons converts inactive synapses to active ones. This Ca2+-CaM-induced conversion was also seen at unitary synapses in pyramidal-to-interneuron pairs (n = 4; J.-H.Wang & J. Wei, unpublished data).
We switched the holding potential from -70 to +50 mV in four experiments of Ca2+-CaM perfusion and observed outward EPSCs at +50 mV. CNQX (10 μm) blocked EPSCs at -70 mV in all four experiments and outward EPSCs at +50 mV in three experiments (an example in Fig. 6a). However, one neuron in CNQX displayed outward EPSCs at +50 mV that were sensitive to 40 μm DAP-5 (inset in b2 of Fig. 2B). It is not clear why NMDAR-mediated EPSCs were not detected in most of the non-pyramidal neurons in our studies, and this was different from the results in pyramidal neurons (Isaac et al. 1995; Liao et al. 1995; Durand et al. 1996). We did not record EPSCs at +50 mV in all our experiments of Ca2+-CaM perfusion. In addition to a low chance of detecting NMDAR-mediated EPSCs, membrane currents fluctuated at +50 mV, presumably due to activation of voltage-gated ion channels. It took a few minutes for inactivation of those channels and the disappearance of the fluctuation. As Ca2+-CaM-induced emergence of EPSCs usually occurred in 4 min (Fig. 6a and Fig. 11), we were able to record baseline values of EPSCs at -70 mV.
Ca2+-CaM increases the amplitude and frequency of spontaneous EPSCs in CA1 SP non-pyramidal neurons
We examined the effect of Ca2+-CaM on sEPSCs. Ca2+-CaM were perfused into SP non-pyramidal neurons (PND 18-22), when pipette tips were filled with the standard solution and back-filled with Ca2+-CaM (40:10 μm). sEPSCs were recorded for 4 min immediately after the formation of the whole-cell configuration. After 15 min for the perfusion of Ca2+-CaM into neurons, we recorded sEPSCs for another 4 min. Figure 7 shows the relationship between cumulative probability and sEPSC amplitudes (Fig. 7a) or inter-sEPSC intervals (Fig. 7B). After perfusing Ca2+-CaM, sEPSC amplitudes increased and inter-EPSC intervals shortened (n = 5, filled symbols). Standard error bars show the variation of the cumulative probability in sEPSC amplitude and the intervals among neurons. These results indicate that Ca2+-CaM-induced synaptic potentiation in SP non-pyramidal neurons may require post- and presynaptic mechanisms because a change in sEPSC frequency is believed to be presynaptic in origin and amplitude is a postsynaptic index (Redman, 1990).
If increases in transmitter release occur during the perfusion of Ca2+-CaM into postsynaptic neurons, retrograde messengers are required for post- to presynaptic communication. We examined the possibility that NO acts as a retrograde messenger because Ca2+-CaM activates nitric oxide synthase (NOS; Griffith & Stuehr, 1995). Carboxy-PTIO (30 μm), a NO scavenger (Rand & Li, 1995), was added to the solution of slice perfusion to block post- to presynaptic NO diffusion when we introduced Ca2+-CaM into SP non-pyramidal neurons. This concentration has been shown to block NO action and long-term potentiation (Ko & Kelly, 1999). Ca2+-CaM-induced increases in sEPSC frequency and amplitude were not attenuated by carboxy-PTIO (Fig. 7D-F, n = 5). These results imply that NO is not a retrograde messenger in Ca2+-CaM-induced synaptic potentiation, and the increase in sEPSC frequency may be due to the conversion of inactive synapses to active ones instead of purely presynaptic changes.
Ca2+-CaM signals differentially increase EPSCs on SP and SR non-pyramidal neurons
Tetanic stimulation induced synaptic potentiation in hippocampal CA1 oriens interneurons (Taube & Schwartzkroin, 1987; Ouardouz & Lacaille, 1995) but depression in SR interneurons (McMahon & Kauer, 1997). We have compared the levels of synaptic potentiation induced by adenophostin or Ca2+-CaM in SP and SR non-pyramidal neurons. Adenophostin-induced synaptic potentiation was larger in SP non-pyramidal neurons (377 ± 31 %, n = 9; filled symbols) than in SR (214 ± 13 %, n = 6; Fig. 8a). Similarly, Ca2+-CaM induced larger synaptic potentiation in SP non-pyramidal neurons (277 ± 25 %, n = 11) compared with that in SR (160 ± 9 %, n = 6; open symbols Fig. 8B). These results indicate that the Ca2+-CaM signalling pathway differentially modulates synaptic function in SP and SR non-pyramidal neurons. We have not observed PS-induced synaptic potentiation in SR non-pyramidal neurons (n = 5, data not shown), consistent with a report of Maccaferri & McBain, 1996.
The kinetics of EPSCs in SR non-pyramidal neurons were faster and the amplitude was lower than those in SP (waveforms in Fig. 8). Rising times to EPSC peak in SP and SR non-pyramidal neurons were 3.55 ± 0.07 ms (n = 12) and 1.82 ± 0.09 ms (n = 10, P < 0.001), respectively. In addition, the duration of EPSCs in SR non-pyramidal neurons was shorter (waveforms in Fig. 8). We did not measure decay-time constants since EPSCs recorded in each neuron were generated from a population of synapses and the decay of unitary EPSCs in pyramidal-to-interneuron pairs was variable among synapses (n = 10; J.-H. Wang & J. Wei, unpublished observations, data not shown). Differences in EPSC kinetics and amplitudes imply that these two groups of non-pyramidal neurons contain different subtypes of synaptic AMPARs and/or densities of synapses.
Postnatal changes of synaptic potentiation induced by PS, adenophostin or Ca2+-CaM
We compared levels of synaptic potentiation in CA1 SP non-pyramidal neurons during postnatal development. Figure 9a shows the effect of PS on EPSCs in PND 7-9, 11-13 and 18-22. The level of potentiation was higher in PND 18-22 (199 ± 15 %, n = 8, filled symbols) than in PND 11-13 (135 ± 10 %, n = 7, grey symbols). PS did not induce synaptic potentiation in PND 7-9 (94 ± 10 %, n = 7, open symbols). Similarly, adenophostin-induced synaptic potentiation shows postnatal changes in magnitude (Fig. 10). Levels of synaptic potentiation at 30 min were 126 ± 7 % in PND 10-11 (open symbols, n = 5), 212 ± 16 % in PND 13-15 (grey symbols, n = 6) and 373 ± 31 % in PND 19-21 (filled symbols, n = 9). The magnitude of PS- or adenophostin-induced synaptic potentiation was significantly different among the three ages (P < 0.01).
In addition to PND 18-22, we examined whether Ca2+-CaM could induce the emergence of EPSCs in young rats using the same protocol as Fig. 6. Figure 11 shows that EPSCs emerged during perfusing Ca2+-CaM. Probabilities of evoked EPSCs (before vs. after) perfusion were 0.11 ± 0.02 vs. 0.48 ± 0.06 in PND 9-11 and 0.17 ± 0.02 vs. 0.67 ± 0.04 in PND 13-15, respectively. Increases in probability were significant in each of these ages (P < 0.001), indicating that Ca2+-CaM converts inactive to active synapses in young rats. We also compared the maximum amplitude and probability of emerged EPSCs among the three ages. By analysing data in PND 9-11 (top panels, n = 11), 13-15 (middle panels, n = 11) and 18-22 (bottom panels, n = 12), we found that the maximum amplitudes of emerged EPSCs were 24.9 ± 2.7, 40 ± 4.7 and 62 ± 5.1 pA, respectively (P < 0.05). The probability of emerged EPSCs are significantly different in the three groups (P < 0.05, Fig. 11). These results indicate that postsynaptic Ca2+-CaM increases the conversion rate of inactive-to-active synapses during postnatal development.
We have investigated the influence of the Ca2+-CaM signalling pathway on synaptic transmission in hippocampal CA1 non-pyramidal neurons, and used paired stimuli, adenophostin (IP3R agonist) and Ca2+-CaM to activate this pathway. These three protocols induced synaptic potentiation in SP non-pyramidal neurons, and underlying mechanisms included the conversion of inactive to active synapses and developmental maturation.
It has been suggested that tetanic stimulation induced synaptic potentiation in hippocampal CA1 interneurons through polysynaptic mechanisms (Maccaferri & McBain, 1996). However, we used paired stimuli (Kelso et al. 1986; Maccaferri & McBain, 1996) which are believed to change synaptic strength in recording neurons. Taking this together with the short latency of EPSCs (waveforms in Figs 1-3), we suggest that the potentiation in SP non-pyramidal neurons is monosynaptic.
Paired stimuli-induced synaptic potentiation required an increase in postsynaptic calcium (Fig. 2a). Intracellular Ca2+ may rise through the activation of NMDAR-channels (Mayer et al. 1989; Jahr & Stevens, 1990), voltage-gated calcium channels (Fox et al. 1987; Tsien et al. 1988) and/or Ca2+ releases (Ehrlich, 1995; Berridge, 1998; Fagni et al. 2000). We observed that DAP-5 (40 μm) partially attenuated PS-induced synaptic potentiation, indicating that NMDARs are not the sole Ca2+ provider for the potentiation in non-pyramidal neurons. The NMDAR-independent portion of PS-induced potentiation may depend upon Ca2+ released from intracellular stores, because adenophostin (an agonist of IP3Rs) induced synaptic potentiation and occluded the effect of paired stimuli (Fig. 4).
Paired stimuli-, adenophostin- or Ca2+-CaM-induced synaptic potentiation was blocked by introducing inhibitors of the Ca2+-CaM signalling pathway (Figs 3-5) into postsynaptic neurons, indicating an involvement of CaM-dependent protein kinases. Adenophostin- or Ca2+-CaM-induced synaptic potentiation occluded PS-induced potentiation (Figs 4-5). Therefore, the Ca2+-CaM signalling pathway is one of the essential signalling cascades to synaptic potentiation in SP non-pyramidal neurons. However, compared with PS- and Ca2+-CaM-induced synaptic potentiation, the level of adenophostin-induced potentiation was higher and the rising phase was shorter. Our interpretation for these differences is that adenophostin may greatly increase Ca2+ levels that activate other Ca2+ signalling pathways besides CaM. It is noteworthy that PS-induced synaptic potentiation developed slowly (Fig. 1 and Fig. 9), because the potentiation did not contain a component of post-tetanic potentiation, a short-term synaptic plasticity (Zucker, 1989).
We first demonstrate the presence of Ca2+-CaM signalling pathways in hippocampal CA1 non-pyramidal neurons using physiological methods. The α-subunit of CaM-KII was not detected in hippocampal CA1 GABAergic interneurons with immunocytochemistry (Liu & G, 1996; Sik et al. 1998), but small amounts of enzymes below detection threshold might still be functional (McBain et al. 1999). Compared to electrophysiology, immunocytochemistry may be a less sensitive method. For example, the maturation of AMPAR-containing synapses was detected by a physiological approach in PND 6 (Durand et al. 1996) whereas AMPARs were detected by immunocytochemistry after postnatal week 2 (Petralia et al. 1999). On the other hand, if αCaMKII is not present in interneurons, other types of CaM-dependent protein kinases, βCaM-KII, CaMKI and CaMKIV (Schulman & Lou, 1989), may be involved in synaptic potentiation. Being unable to identify if non-pyramidal neurons in our studies were GABAergic, we cannot conclude whether our results differ from those in previous immunocytochemical studies (Liu & Jones, 1996; Sik et al. 1998). However, we believe that the most of hippocampal interneurons are GABAergic (Freund & Buzsaki, 1996).
Our results indicate that Ca2+-CaM signalling pathways in CA1 SP non-pyramidal neurons act on postsynaptic targets and convert inactive synapses to active ones. As AMPARs are enriched in the postsynaptic membrane of hippocampal interneurons (Nusser et al. 1998), the inactivity of synapses may be due to the non-functional status of receptors. Ca2+-CaM signalling cascades enhance the function of AMPARs and then convert inactive to active synapses in non-pyramidal neurons. The conversion of silent synapses to functional ones was suggested as one of mechanisms in long-term potentiation (Isaac et al. 1995; Liao et al. 1995), in which AMPAR-mediated EPSCs emerged in NMDAR-containing synapses. However, inactive synapses in our studies seem to be different from silent synapses in pyramidal neurons. Most of the inactive synapses in SP non-pyramidal neurons did not show NMDAR-mediated EPSCs (Fig. 6). Their functional status was facilitated by paired pulses, and such a protocol was not used in previous studies (Isaac et al. 1995; Liao et al. 1995). Although the receptor composition of inactive synapses in non-pyramidal neurons may be different from that of silent synapses in pyramidal neurons, they appear to be similar in the emergence of AMPAR-mediated EPSCs during synaptic potentiation.
Changes in sEPSC frequency usually indicate presynaptic phenomena (Redman, 1990). Increases in sEPSC frequency during the postsynaptic perfusion of Ca2+-CaM could be interpreted as a presynaptic involvement. However, a NO scavenger had little effect on Ca2+-CaM-induced increases in sEPSC frequency (Fig. 7), implying that NO and presynaptic mechanisms are not involved. An alternative postsynaptic mechanism might be the conversion of inactive to active synapses because increases in the number of postsynaptic AMPARs were associated with an increase in sEPSC frequency (Petralia et al. 1999). The addition of more functional receptors increases the chance for a transmitter to evoke more synaptic events such that the probability of evoked EPSCs and the frequency of sEPSCs increase. Changes in sEPSC amplitude and frequency may also be an index of inactive-to-active synapse conversion that is based on postsynaptic mechanisms.
The magnitude of PS-, adenophostin- and Ca2+-CaM-induced synaptic potentiation on SP non-pyramidal neurons in hippocampal area CA1 increased during postnatal development (Figs 9-11). As underlying mechanisms appear postsynaptic in origin, postnatal changes may result from quantitative increases in intracellular Ca2+-CaM targets and/or postsynaptic receptors. It is not clear whether the number of synaptic AMPARs in non-pyramidal neurons changes during development, similar to that in pyramidal neurons (Durand et al. 1996; Liao et al. 1999; Petralia et al. 1999). As the expression of protein kinase development rises (Huang, 1989; Kelly, 1992), postnatal changes appear, probably due to the quantitative increase in Ca2+-CaM signalling cascades. AMPARs in non-pyramidal neurons may primarily be at a low functional status. When Ca2+-CaM signalling cascades mature, their modulation of synaptic targets may increase the ratio of functional to non-functional AMPARs and the conversion rate of inactive-to-active synapses over early postnatal weeks.
We investigated synaptic plasticity in hippocampal CA1 SP non-pyramidal neurons that are important in controlling the excitatory activity of pyramidal neurons (Freund & Buzsaki, 1996). The activation of postsynaptic Ca2+-CaM signalling cascades by pairied stimuli, evoking Ca2+ release or perfusing Ca2+-CaM induces monosynaptic potentiation in these neurons. The physiological consequences of the up-regulation of glutamatergic synapses in interneurons would be to increase their activity and enable them to inhibit principal neurons more efficiently.
We thank Dr M. Takahashi for adenophostin, Ms J. Wei for experimental assistance and Dr E. Floor for reading the final version. We especially thank the editors of The Journal of Physiology for help with correcting the writing. This study was supported by University of Kansas startup funds to J.H.W. and NS32470 to P.T.K.