Chapter 10.1 Introduction to cryocrystallography

Crystallography of biological macromolecules

First Online Edition (2006)

Part 10. Cryocrystallography

  1. H. Hope

Published Online: 1 JAN 2006

DOI: 10.1107/97809553602060000672

International Tables for Crystallography

International Tables for Crystallography

How to Cite

Hope, H. 2006. Introduction to cryocrystallography. International Tables for Crystallography. F:10:10.1:197–201.

Author Information

  1. Department of Chemistry, University of California, Davis, One Shields Ave, Davis, CA 95616‐5295, USA

Publication History

  1. Published Online: 1 JAN 2006



A general introduction to cryocrystallography is provided. The chapter covers crystal mounting, cooling of biocrystals, principles of cooling equipment and frost prevention. Operational considerations, such as dual‐stream instruments, electrically heated nozzles, temperature calibration and transfer of crystals to difractometers, are also described.


  • cryocrystallography;
  • crystal mounting;
  • data collection;
  • frost prevention;
  • ice formation;
  • radiation damage