Chapter 19.1 Neutron crystallography: methods and information content

Crystallography of biological macromolecules

First Online Edition (2006)

Part 19. Other experimental techniques

  1. A. A. Kossiakoff

Published Online: 1 JAN 2006

DOI: 10.1107/97809553602060000698

International Tables for Crystallography

International Tables for Crystallography

How to Cite

Kossiakoff, A. A. 2006. Neutron crystallography: methods and information content. International Tables for Crystallography. F:19:19.1:419–422.

Author Information

  1. Department of Biochemistry and Molecular Biology, CLSC 161A, University of Chicago, Chicago, IL 60637, USA

Publication History

  1. Published Online: 1 JAN 2006



The application of the neutron‐diffraction technique, which assigns H‐atom positions in proteins and differentiates between H and D atoms, has been mainly focused on structural issues in three research areas: protein reaction mechanisms; protein dynamics; and protein–water interactions. Topics covered in this chapter include: diffraction geometries; the information content of neutron density maps; phasing models and evaluation of correctness; methodologies employed to refine neutron data; and D2O − H2O difference maps and their applications.


  • D2O − H2O difference maps;
  • hydrogen/deuterium exchange;
  • neutron density maps;
  • neutron diffraction;
  • phasing;
  • quasi‐Laue neutron diffraction;
  • refinement;
  • solvent structure;
  • water molecules