Chapter

Chapter 19.10 Single‐particle reconstruction with EMAN

Crystallography of biological macromolecules

Second Online Edition (2012)

Part 19. Other experimental techniques

  1. S. Ludtke

Published Online: 14 APR 2012

DOI: 10.1107/97809553602060000876

International Tables for Crystallography

International Tables for Crystallography

How to Cite

Ludtke, S. 2012. Single‐particle reconstruction with EMAN. International Tables for Crystallography. F:19:19.10:629–632.

Author Information

  1. National Center for Macromolecular Imaging, Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA

Publication History

  1. Published Online: 14 APR 2012

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Abstract

Single‐particle reconstruction is a technique for determining the three‐dimensional structure of large sets of identical nanoscale objects, typically proteins or macromolecular assemblies, using electron cryomicroscopy (cryo‐EM). This technique has now demonstrated resolutions of 3–5 Å for a number of targets and can routinely achieve subnanometre resolution. EMAN is a scientific image‐processing software suite first developed to make single‐particle reconstructions more reproducible and less labour‐intensive. While it has since expanded to include many algorithms for other types of image processing, providing robust single‐particle reconstructions with minimal effort remains one of its primary focuses. It includes a complete workflow infrastructure for this and other tasks in cryo‐EM image analysis and structural biology.

Keywords:

  • EMAN;
  • single‐particle reconstruction;
  • electron cryomicroscopy