Biotechnology and Bioengineering

Cover image for Vol. 111 Issue 12

Edited By: Douglas S. Clark

Impact Factor: 4.164

ISI Journal Citation Reports © Ranking: 2013: 26/165 (Biotechnology & Applied Microbiology)

Online ISSN: 1097-0290

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  • Lessons from diversity of directed evolution experiments by an analysis of 3,000 mutations

    Lessons from diversity of directed evolution experiments by an analysis of 3,000 mutations

    Amino acid substitutions at aa-positions 131–136 which are generated by (a) epPCR-low; (b) epPCR-high; and (c) SeSaM-Tv P/P. The BSLA wild-type sequence is given below.

  • Engineered thermostable fungal cellulases exhibit efficient synergistic cellulose hydrolysis at elevated temperatures

    Engineered thermostable fungal cellulases exhibit efficient synergistic cellulose hydrolysis at elevated temperatures

    Synergistic cellulose hydrolysis by wild type (A, C) and engineered thermostable (B, D) Cel5A, Cel6A, and Cel7A. Reactions contained a total concentration of 0.5 µM of cellulase and 1% w/v Avicel. Edges are labeled with the concentration of the noted cellulase, which ranges from 0% to 100% of the total. Each vertex represents 100% of an individual cellulase, each edge represents a mixture of two cellulases, and the interior of the triangle represents a mixture of all three cellulases. Black dots are individual measurements (in duplicate), and colors are arithmetic averages between each point, with red representing maximum activity and blue representing minimum activity. Colors are normalized for each synergism test. The absolute activities of the individual enzymes as well as the best mixtures for double and triple enzyme combinations are shown for wild type (C) and engineered thermostable enzymes (D).

  • Multilevel correlations in the biological phosphorus removal process: From bacterial enrichment to conductivity-based metabolic batch tests and polyphosphatase assays

    Multilevel correlations in the biological phosphorus removal process: From bacterial enrichment to conductivity‐based metabolic batch tests and polyphosphatase assays

    Hierarchical clustering and principal component analysis (PCA) of cultivation conditions retrieved from 35 reference studies (Table ) selecting for either PAOs or GAOs in anaerobic-aerobic SBRs. The numbered reference studies were clustered according to the reported relative abundances of PAOs and GAOs (A). According to the first PCA correlation biplot (B) the first blue and third orange clusters are related to predominance of PAOs (relative abundance >60% and PAO/GAO ratio >4) and GAOs (relative abundance >50% and PAO/GAO ratio <1), respectively, whereas the second grey cluster is related to intermediate enrichments. These gradients in PAO and GAO abundances across the studies are displayed in Table . The second PCA correlation biplot (C) provides information on the directions of correlation between the cultivation conditions and the relative abundance of PAOs and GAOs. The direction and length of vectors indicate the extent of correlations between operation variables and reference studies. Pairwise Spearman's rank correlation coefficients computed between PAO and GAO fractions and operation variables are presented in a heatmap (D). The dendrograms display clusters of variables that orient bacterial selection in the same direction. Among the 11 variables ranked here, the presence of propionate in the cultivation medium is a key factor selecting for PAOs at the expense of GAOs (strong inverse correlation).

  • Biofilm growth of Chlorella sorokiniana in a rotating biological contactor based photobioreactor

    Biofilm growth of Chlorella sorokiniana in a rotating biological contactor based photobioreactor

    Schematic overview of Algadisk lab scale reactor. D = disk, M = motor driving the disk, C = container, T = temperature control system, BT = buffer tank. Both the top of the container and buffer tank are open. Liquid in the reactor vessel flows from left to right, and the direction of disk rotation is depicted in the figure.

  • Using an aqueous two-phase polymer-salt system to rapidly concentrate viruses for improving the detection limit of the lateral-flow immunoassay

    Using an aqueous two‐phase polymer‐salt system to rapidly concentrate viruses for improving the detection limit of the lateral‐flow immunoassay

    Schematic of the LFA sandwich assay. Positive results (top) are indicated by two red bands, while negative results (bottom) are indicated by a single red band.

  • Electrospun meshes possessing region-wise differences in fiber orientation, diameter, chemistry and mechanical properties for engineering bone-ligament-bone tissues

    Electrospun meshes possessing region‐wise differences in fiber orientation, diameter, chemistry and mechanical properties for engineering bone‐ligament‐bone tissues

    Cartoons of electrospinning set-up depicting the offset spinnerets and the dual-drum collector. (a) Two spinnerets were used to form a single transition region. (b) Three spinnerets were used to form two transition regions. Shields (in brown) were used to control the sizes of the transition regions.

  • Spatial optimization in perfusion bioreactors improves bone tissue-engineered construct quality attributes

    Spatial optimization in perfusion bioreactors improves bone tissue‐engineered construct quality attributes

    Schematic representation of sequential scaffold locations from Lentr to Lentr + jΔL (ΔL = 500 µm) for which CFD simulations were run. Planar sections represent scaffold entrance and exit and arrows velocity vectors. ΔuLentr was calculated from Equation while as a convergence criterion for the determination of Lcrit the following was used: |ΔuLentr + (j−1) ΔL − ΔuLentr + jΔL| = 0. At that point the Lcrit was determined for each flow rate.

  • Lessons from diversity of directed evolution experiments by an analysis of 3,000 mutations
  • Engineered thermostable fungal cellulases exhibit efficient synergistic cellulose hydrolysis at elevated temperatures
  • Multilevel correlations in the biological phosphorus removal process: From bacterial enrichment to conductivity‐based metabolic batch tests and polyphosphatase assays
  • Biofilm growth of Chlorella sorokiniana in a rotating biological contactor based photobioreactor
  • Using an aqueous two‐phase polymer‐salt system to rapidly concentrate viruses for improving the detection limit of the lateral‐flow immunoassay
  • Electrospun meshes possessing region‐wise differences in fiber orientation, diameter, chemistry and mechanical properties for engineering bone‐ligament‐bone tissues
  • Spatial optimization in perfusion bioreactors improves bone tissue‐engineered construct quality attributes

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