Biotechnology and Bioengineering

Cover image for Vol. 111 Issue 5

Edited By: Douglas S. Clark

Impact Factor: 3.648

ISI Journal Citation Reports © Ranking: 2012: 31/160 (Biotechnology & Applied Microbiology)

Online ISSN: 1097-0290

Featured

  • Electrical conditioning of adipose-derived stem cells in a multi-chamber culture platform

    Electrical conditioning of adipose-derived stem cells in a multi-chamber culture platform

    a: Cell culture platform consisting of two PMMA chassis, each of which is capable of hosting six independent culture chambers, and a custom-made electrical stimulator. The locking/unlocking system securing the chambers is observable on the basement of the chassis. The back panel of each chassis embeds the electrical connections for connecting the culture chamber electrodes to the electrical stimulator. b: Rectangular-shaped PDMS culture chamber with two embedded AISI 316 electrodes and its lid. c: Mold manufactured for producing PDMS culture chambers using a mold casting and curing technique. The mold consisted of two PMMA elements (transparent) and three POM elements (white).

  • Quantitative assessment of in vivo HIV protease activity using genetically engineered QD-based FRET probes

    Quantitative assessment of in vivo HIV protease activity using genetically engineered QD-based FRET probes

    Protein probes employed in this work. A: Presence of active HIV-1 Pr disrupts FRET resulting in an increase in the QD emission and a corresponding decrease in the Alexa emission. B: The different cleavage sequences employed in this work.

  • Effects of scaffold architecture on mechanical characteristics and osteoblast response to static and perfusion bioreactor cultures

    Effects of scaffold architecture on mechanical characteristics and osteoblast response to static and perfusion bioreactor cultures

    Static culture: FDA (live, green)/PI (dead, red) fluorescent stain on Day 7—Z-stack of between 40 and 60 images (a–d); DAPI (blue)/phalloidin (rhodamine, red); nuclei/actin stain on Day 7—Maximum projection images (e–h); SEM image of cells on scaffold filament junctions on Day 7 using 20 kV and 200× magnification (i–l).

  • Identification of Staphylococcus aureus α-hemolysin as a protein drug that is secreted by anticancer bacteria and rapidly kills cancer cells

    Identification of Staphylococcus aureus α-hemolysin as a protein drug that is secreted by anticancer bacteria and rapidly kills cancer cells

    Ideal characteristics of a bacterially delivered protein for cancer therapy. A: An ideal toxin should be small, secrete out of bacteria, and act externally on cancer cells to cause death, either through membrane disruption or unassisted internalization into cells. B: Bacterial vector that can produce a toxin after direct induction with L-arabinose. This plasmid contains the ampicillin resistance gene; the pBAD promoter, which responds to arabinose; the araC regulator gene; and the gene encoding the desired toxin. Multi-cloning sites (MCS) at the 5′ and 3′ ends were used to insert toxin genes.

  • High-throughput biosensor discriminates between different algal H2-photoproducing strains

    High-throughput biosensor discriminates between different algal H2-photoproducing strains

    The detection of 1 in 10,000 H2-producing colonies. The left plate shows an overlay response of H2-positive, D66 C. reinhardtii colonies in a 10,000-fold excess of the hydrogenase-less hydG colonies. The right plate shows the opposite ratio, where H2-producing colonies are in 10,000-fold excess. Plates were imaged at the same sensitivity settings for comparison.

  • Electrical conditioning of adipose-derived stem cells in a multi-chamber culture platform
  • Quantitative assessment of in vivo HIV protease activity using genetically engineered QD-based FRET probes
  • Effects of scaffold architecture on mechanical characteristics and osteoblast response to static and perfusion bioreactor cultures
  • Identification of Staphylococcus aureus α-hemolysin as a protein drug that is secreted by anticancer bacteria and rapidly kills cancer cells
  • High-throughput biosensor discriminates between different algal H2-photoproducing strains

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