Microscopy Research and Technique

Cover image for Vol. 79 Issue 8

Edited By: Alberto Diaspro

Online ISSN: 1097-0029

Virtual Issues


Virtual Issues from Microscopy Research and Technique



Nobel Laureate Stefan W. Hell

Published: 13 October 2014

Microscopy Research & Technique congratulates Stefan W. Hell for winning the 2014 Nobel Prize in Chemistry. We are honored that Prof. Hell has chosen MRT to publish his research, which is collected below and is freely available until the end of 2014.

Foreword from Stefan W. Hell: My collaborators and I are very proud of our papers in MRT. Some of them became references for the current and future generations of students in super resolution fluorescence microscopy.

Monitoring triplet state dynamics with fluorescence correlation spectroscopy: Bias and correction
Andreas Schönle, Claas Von Middendorff, Christian Ringemann, Stefan W. Hell and Christian Eggeling
Microsc. Res. Tech., 2014, 77: 528–536

MRT letter: Nanoscopy of protein colocalization in living cells by STED and GSDIM
Birka Lalkens, Ilaria Testa, Katrin I. Willig and Stefan W. Hell
Microsc. Res. Tech., 2012, 75: 1–6

3D reconstruction of high-resolution STED microscope images
Annedore Punge, Silvio O. Rizzoli, Reinhard Jahn, Jan Dominik Wildanger, Lars Meyer, Andreas Schönle, Lars Kastrup and Stefan W. Hell
Microsc. Res. Tech., 2008, 71: 644–650

Reversible photoswitching enables single-molecule fluorescence fluctuation spectroscopy at high molecular concentration
C. Eggeling, M. Hilbert, H. Bock, C. Ringemann, M. Hofmann, A.C. Stiel, M. Andresen, S. Jakobs, A. Egner, A. Schönle and S.W. Hell
Microsc. Res. Tech., 2007, 70: 1003–1009

Wide-field subdiffraction RESOLFT microscopy using fluorescent protein photoswitching
Miriam A. Schwentker, Hannes Bock, Michael Hofmann, Stefan Jakobs, Jörg Bewersdorf, Christian Eggeling and Stefan W. Hell
Microsc. Res. Tech., 2007, 70: 269–280

2,2′-Thiodiethanol: A new water soluble mounting medium for high resolution optical microscopy
Thorsten Staudt, Marion C. Lang, Rebecca Medda, Johann Engelhardt and Stefan W. Hell
Microsc. Res. Tech., 2007, 70: 1–9

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Innovations and Applications from MRT

Published: 03 Aug 2012

Edited By: Prof. George C. Ruben, Editor-in-Chief

Microscopy Research & Technique (MRT) publishes articles across the spectrum of microscopy techniques and also features research applying these diverse methods to biology, materials science, chemistry, and medicine, among other fields. This Virtual Issue highlights some of MRT’s latest cutting-edge research, including an innovative application in conservation science, a new illumination technique for improved visualizations of complex structured transparent specimens, and a novel system to efficiently segment brain MR images.

Readers of MRT will always find something new, relevant, and provocative, both within and outside their specialties.
-Prof. George C. Ruben Editor-in-Chief, MRT

Mechanosensory neurons, cutaneous mechanoreceptors, and putative mechanoproteins
M.E. Del Valle, T. Cobo, J.L. Cobo and J.A. Vega

A reproducible method for damage-free site-specific preparation of atom probe tips from interfaces
Peter Johann Felfer, Talukder Alam, Simon Peter Ringer and Julie Marie Cairney

Combined in vivo multiphoton and CARS imaging of healthy and disease-affected human skin
Hans Georg Breunig, Rainer Bückle, Marcel Kellner-Höfer, Martin Weinigel, Jürgen Lademann, Wolfram Sterry and Karsten König

Tumor detection from enhanced magnetic resonance imaging using fuzzy curvelet
M. Arfan Jaffar, Quratul Ain and Tae Sun Choi

Variable bright-darkfield-contrast, a new illumination technique for improved visualizations of complex structured transparent specimens
Timm Piper and Jörg Piper

Biosensor Förster resonance energy transfer detection by the phasor approach to fluorescence lifetime imaging microscopy
Elizabeth Hinde, Michelle A. Digman, Christopher Welch, Klaus M. Hahn and Enrico Gratton

Fluorescence recognition of proteinaceous binders in works of art by a novel integrated system of investigation
Irina Crina Anca Sandu, Ana Cecilia A. Roque, Paolo Matteini, Stephan Schäfer, Giovanni Agati, Catarina Ribeiro Correia and Joana Fortio Fernandes Pacheco Viana

MRT letter: Nanoscopy of protein colocalization in living cells by STED and GSDIM
Birka Lalkens, Ilaria Testa, Katrin I. Willig and Stefan W. Hell

MRT letter: Contrast-enhanced computed tomographic imaging of soft callus formation in fracture healing
Lauren Nicole Miller Hayward, Chantal Marie-Jeanne DE Bakker, Hrvoje Lusic, Louis Charles Gerstenfeld, Mark W. Grinstaff and Elise Feng-i Morgan

An imaging technique using rotational polarization microscopy for the microstructure analysis of carbon/carbon composites
Li Miaoling, Qi Lehua and Li Hejun


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Innovative Microscopy Techniques for Biological Research

Published: 05 Apr 2011

Edited By: Prof. George C. Ruben, Editor-in-Chief

Introduction: Read the Editor's introduction to this Virtual Issue.

Novel Lambda FRET Spectral Confocal Microscopy Imaging Method
Diego Megías, Raquel Marrero, Borja Martínez del Peso, María Ángel García, José-Javier Bravo-Cordero, Aranzazu García-Grande, Andrés Santos and María C. Montoya

Optimized Protocol of a Frequency Domain Fluorescence Lifetime Imaging Microscope for FRET Measurements
Aymeric Leray, Franck B. Riquet, Elodie Richard, Corentin Spriet, Dave Trinel and Laurent Héliot

Fluorescence Recovery After Photobleaching and Photoconversion in Multiple Arbitrary Regions of Interest Using a Programmable Array Microscope
Guy M. Hagen, Wouter Caarls, Keith A. Lidke, Anthony H.B. De Vries, Cornelia Fritsch, B. George Barisas, Donna J. Arndt-Jovin and Thomas M. Jovin

Analysis of Diffusion and Binding in Cells Using the RICS Approach
Michelle A. Digman and Enrico Gratton

Accurate Shape From Focus Based on Focus Adjustment in Optical Microscopy
Seong-O Shim, Aamir Saeed Malik and Tae-Sun Choi

The Influence of Chromatic Components on Extended Depth of Field Imaging
Javier Navas and Joaquín Martín

Demonstration of High Lateral Resolution in Laser Confocal Microscopy Using Annular and Radially Polarized Light
Jeongyong Kim, Dae-Chul Kim and Seng-Hun Back

Validation of Two Dual Fluorescence Techniques for Confocal Microscopic Visualization of Resin Penetration Into Enamel Caries Lesions
Sebastian Paris, Kerstin Bitter, Herbert Renz, Werner Hopfenmuller and Hendrik Meyer-Lueckel

Morphological Filter Improve the Efficiency of Automated Tracking of Secretory Vesicles With Various Dynamic Properties
Tien-Chuan Ku, Lung-Sen Kao, Chung-Chih Lin and Yuh-Show Tsai

Electron Tomography Study of Isolated Human Centrioles
Rana Ibrahim, Cédric Messaoudi, Francisco Javier Chichon, Claude Celati and Sergio Marco


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Microscopy Techniques in Materials Science

Published: 05 Apr 2011

Edited By: Prof. George C. Ruben, Editor-in-Chief

Introduction: Read the Editor's introduction to this Virtual Issue.

Two-Probe Electrical Measurements in Transmission Electron Microscopes-Behavioral Control of Tungsten Microwires
Pedro M.F.J. Costa, Xiaosheng Fang, Shiliang Wang, Yuehui He, Yoshio Bando, Masanori Mitome, Jin Zou, Han Huang and Dmitri Golberg

Laser-Based In Situ Techniques: Novel Methods for Generating Extreme Conditions in TEM Samples
Mitra L. Taheri, Thomas Lagrange, Bryan W. Reed, Michael R. Armstrong, Geoffrey H. Campbell, William J. DeHope, Judy S. Kim, Wayne E. King, Daniel J. Masiel and Nigel D. Browning

Femtosecond Electron Diffraction: Direct Probe of Ultrafast Structural Dynamics in Metal Films
Shouhua Nie, Xuan Wang, Junjie Li, Richard Clinite and Jianming Cao

Advances in Atomic Resolution In Situ Environmental Transmission Electron Microscopy and 1Å aberration corrected in situ electron microscopy
Pratibha L. Gai and Edward D. Boyes

A New MEMS-Based System for Ultra-High-Resolution Imaging at Elevated Temperatures
Lawrence F. Allard, Wilbur C. Bigelow, Miguel Jose-Yacaman, David P. Nackashi, John Damiano and Stephen E. Mick

In Situ SEM Indentation Experiments: Instruments, Methodology, and Applications
Rudy Ghisleni, Karolina Rzepiejewska-Malyska, Laetitia Philippe, Patrick Schwaller and Johann Michler

Advances in Transmission Electron Microscopy: In Situ Straining and In Situ Compression Experiments on Metallic Glasses
Jeff Th.M. De Hosson

In Situ Deformation of Thin Films on Substrates
Marc Legros, Martiane Cabié and Daniel S. Gianola

Integrated Analytical Study for the Authentication of Five Russian Icons (XVI-XVII centuries)
Irina Crina Anca Sandu, Susanna Bracci, Ion Sandu and Mariella Lobefaro

MRT Letter: In Situ Observation Method for Microstructural Changes of Steel During Hot Deformation
Yasuhiro Yogo, Hirohisa Takeuchi, Kouji Tanaka, Noritoshi Iwata, Koukichi Nakanishi and Takashi Ishikawa


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Important Contributions to Microscopy Research from China

Published: 18 Jan 2011

Edited By: Prof. George C. Ruben, Editor-in-Chief, MRT

Introduction: Read the Editor's introduction to this Virtual Issue.

Different Expression of NR2B and PSD-95 in Rat Hippocampal Subregions During Postnatal Development
Li-Rong Chang, Jin-Ping Liu, Ning Zhang, Yong-Jun Wang, Xiu-Lai Gao and Yan Wu

Quantitative X-Ray Microanalysis of Hydrogen Peroxide Within Plant Cells
Shaoliang Chen, Andrea Olbrich, Rosemarie Langenfeld-Heyser, Eberhard Fritz and Andrea Polle

A Novel and Effective Multistage Classification System for Microscopic Starch Grain Images
Siu-Kai Choy, Chong-Sze Tong and Zhong-Zhen Zhao

Time-Series Investigation of Fused Vesicles in Microvessel Endothelial Cells With Atomic Force Microscopy
Long Chen, Weiguo Chu, Yonggang Xu, Peipei Chen, Fang Lao, Quanmei Sun, Xizeng Feng and Dong Han

Micro-Fabrication and Monitoring of Three-Dimensional Microstructures Based on Laser-Induced Thermoplastic Formation
Leyan Wang, Dongxian Zhang, Zhenghu Wen and Haijun Zhang

An Automatic Integrated Approach for Stained Neuron Detection in Studying Neuron Migration
Yue Huang, Xuezhi Sun and Guangshu Hu

Research on Precision-Calibration Techniques for Selected Area Electron Diffraction Patterns of Pyrocarbon
Lehua Qi, Miaoling Li, Hejun Li, Guozhong Xu and Chuang Wang

Self-Assembled Monolayers of DNA on Cysteamine Modified Au(111) Surface: Atomic Force Microscopy Study
Yonghai Song, Wenping Lian, Sucai Zhao, Li Wang and Zhuang Li

Well-Ordered Structure of Methylene Blue Monolayers on Au(111) Surface: Electrochemical Scanning Tunneling Microscopy Studies
Yonghai Song and Li Wang

Authentication of the 31 Species of Toxic and Potent Chinese Materia Medica by Light Microscopy, Part 3: Two Species of T/PCMM From Flowers and Their Common Adulterants
Chu Chu, Li Xia, Li-Ping Bai, Qin Li, Ping Li, Hu-Biao Chen and Zhong-Zhen Zhao



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