Biotechnology and Bioengineering

Cover image for Vol. 112 Issue 1

Edited By: Douglas S. Clark

Impact Factor: 4.164

ISI Journal Citation Reports © Ranking: 2013: 26/165 (Biotechnology & Applied Microbiology)

Online ISSN: 1097-0290

Featured

  • Modeling enzymatic hydrolysis of lignocellulosic substrates using fluorescent confocal microscopy II: Pretreated biomass

    Modeling enzymatic hydrolysis of lignocellulosic substrates using fluorescent confocal microscopy II: Pretreated biomass

    Overlay of the green (autofluorescence) and deconvoluted red channel (AF647-Cel7A) at different times during enzymatic hydrolysis of pretreated and untreated hardwood in the presence of 0.15 FPU/mL cellulase at 50°C. Rows A, B, and C show images of optimally pretreated hardwood, partially pretreated hardwood and untreated hardwood, respectively.

  • Packaging guest proteins into the encapsulin nanocompartment from Rhodococcus erythropolis N771

    Packaging guest proteins into the encapsulin nanocompartment from Rhodococcus erythropolis N771

    DLS analysis of ReencapsulinHis. The concentration of ReencapsulinHis used was 0.50 mg/mL. Black, red, light green, orange, cyan, and blue lines represent the DLS curves measured at 35, 45, 50, 55, 60, and 65°C, respectively. The polydispersity indexs of the curves are 0.022, 0.069, 0.031, 1.00, 0.785, and 0.814, respectively. Detailed procedures are described in the materials and methods section.

  • Comparison of genome-wide selection strategies to identify furfural tolerance genes in Escherichia coli

    Comparison of genome‐wide selection strategies to identify furfural tolerance genes in Escherichia coli

    Furfural selections performed with trackable multiplex recombineering libraries. A) Three selection schemes were performed. The first was a plate-based selection on solid minimal medium with furfural. The second was a serial batch transfer selection with a decreasing concentration gradient at each batch (i.e., 1.5 g/l to 1.0 g/l to 0.5 g/l furfural in minimal medium). The third selection was performed with serial batch transfer into constant initial concentration loading (1.0 g/l furfural in minimal medium). BD) Genome-wide fitness plots with allele fitness values from the B) plate-based, C) decreasing concentration gradient, and D) constant concentration selections. Fitness values correspond to location within the chromosome as visualized in a clock-wise fashion. Spike amplitude is proportional to the fitness values of increased fitness alleles. Spike color corresponds to the mutational direction of the allele: red for ‘Up’ and blue for ‘Down.’ Fitness values were calculated based upon the concentration of the allele after selection compared to that from the control (unselected) population.

  • Modeling the nutrient removal process in aerobic granular sludge system by coupling the reactor- and granule-scale models

    Modeling the nutrient removal process in aerobic granular sludge system by coupling the reactor‐ and granule‐scale models

    Fluorescent in situ hybridization (FISH) images of the typical granule cross-sections. A: Yellow cells are Accumulibacter spp. (hybridized with both EUBMIX probes; green and PAOMIX probes; red). B: Yellow cells are Competibacter spp. (hybridized with both EUBMIX probes; green and GAOMIX probes; red). C: Yellow cells are betaproteobacterial ammonia oxidizing bacteria (AOB; hybridized with both EUBMIX probes; green and Nso190 probes; red). D: Yellow cells are Nitrospira spp. (hybridized with both EUBMIX probes; green and Ntspa662 probes; red). Scale bar, 100 µm.

  • Use of a small molecule cell cycle inhibitor to control cell growth and improve specific productivity and product quality of recombinant proteins in CHO cell cultures

    Use of a small molecule cell cycle inhibitor to control cell growth and improve specific productivity and product quality of recombinant proteins in CHO cell cultures

    Selective CDK4/6 inhibition by small molecule compound. (A) Schematic diagram of the cell cycle and function of a selective CDK4/6 specific inhibitor. E2F, E2 transcription factor; DP, E2F dimerization partner; R, restriction checkpoint. (B) Whole cell extracts with or without CCI treatment were assayed for protein levels of phospho-Rb (p-Rb), phospho-ERK (p-ERK), phospho-S6 (p-S6) by Western blot. Rab11 was used as a loading control.

  • Assembly and multiple gene expression of thermophilic enzymes in Escherichia coli for in vitro metabolic engineering

    Assembly and multiple gene expression of thermophilic enzymes in Escherichia coli for in vitro metabolic engineering

    mRNA expression levels of thermophilic glycolytic genes in multi-gene-expression E. coli cells. The RT-PCR data were obtained from at least three independent experiments and are shown as means ± standard deviations.

  • Intravital microscopy of localized stem cell delivery using microbubbles and acoustic radiation force

    Intravital microscopy of localized stem cell delivery using microbubbles and acoustic radiation force

    Acoustic intensity field map of the unfocused 1 MHz piston transducer. Radiation force experiments were performed in the far field at 60 mm from the transducer surface in the area indicated by the dashed box (field of view, FOV). The maximum variation in the acoustic intensity within the FOV was 0.4 dB, corresponding to less than 5% variation in acoustical pressure.

  • Modeling enzymatic hydrolysis of lignocellulosic substrates using fluorescent confocal microscopy II: Pretreated biomass
  • Packaging guest proteins into the encapsulin nanocompartment from Rhodococcus erythropolis N771
  • Comparison of genome‐wide selection strategies to identify furfural tolerance genes in Escherichia coli
  • Modeling the nutrient removal process in aerobic granular sludge system by coupling the reactor‐ and granule‐scale models
  • Use of a small molecule cell cycle inhibitor to control cell growth and improve specific productivity and product quality of recombinant proteins in CHO cell cultures
  • Assembly and multiple gene expression of thermophilic enzymes in Escherichia coli for in vitro metabolic engineering
  • Intravital microscopy of localized stem cell delivery using microbubbles and acoustic radiation force

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