Biotechnology and Bioengineering

Cover image for Vol. 112 Issue 6

Edited By: Douglas S. Clark

Impact Factor: 4.164

ISI Journal Citation Reports © Ranking: 2013: 26/165 (Biotechnology & Applied Microbiology)

Online ISSN: 1097-0290

Featured

  • Excess surface area in bioelectrochemical systems causes ion transport limitations

    Excess surface area in bioelectrochemical systems causes ion transport limitations

    Electrode and reactor assembly. Top Left: Sheath, graphite felt coupon, and titanium wire before assembly. Top Right: Electrode-sheath assembly. Bottom Right: Electrode assembly attached to bulkhead fitting in bottle cap. Bottom Left: Manifold reactor assembly of Adams and Chittenden microbial fuel cells in a water bath.

  • Improved virus purification processes for vaccines and gene therapy

    Improved virus purification processes for vaccines and gene therapy

    Different approaches to increase process knowledge in downstream processing. Mechanistic modeling requires a deep understanding of the physico-chemical property of the system, whereas a data-driven model requires the use of statistical tools such as DoE or Monte Carlo simulations. However, the two approaches can complement each other and provide more robust process knowledge, together with the use of high throughput screening techniques, the integration of all the unit operations and the process economics parameters.

  • A simple and low-cost platform technology for producing pexiganan antimicrobial peptide in E. coli

    A simple and low‐cost platform technology for producing pexiganan antimicrobial peptide in E. coli

    SDS-PAGE result of the purification process of DAMP4var-pexiganan. (Lane 1) Before induction, (lane 2) total protein, (lane 3) soluble protein, (lane 4) precipitate after heating and centrifugation, (lane 5) supernatant after heating and centrifugation, (lane 6) precipitate after dilution to precipitate stage, (lane 7) supernatant after dilution to precipitate stage, and (lane 8) ladder.

  • A high cell density transient transfection system for therapeutic protein expression based on a CHO GS-knockout cell line: Process development and product quality assessment

    A high cell density transient transfection system for therapeutic protein expression based on a CHO GS‐knockout cell line: Process development and product quality assessment

    Comparison of direct versus indirect (pre-complexed) transfection. Cells were centrifuged and re-suspended in transfection media at a density of 4 × 106/mL and varying amounts of DNA and PEI were added as indicated. The cultures were incubated at 32°C. For direct transfection (Fig. A, C, and E), DNA and PEI were sequentially added to the culture. For indirect transfection (Fig. B, D, and F) DNA and PEI were added to ∼10% of transfection media and allowed to pre-complex for 15–20 min before adding to the cells. (A, B) Transfection efficiency was determined 48 h post transfection by measuring %GFP positive cells by flow-cytometer. (C, D) Mean GFP expression level (arbitrary units) was measured 24 h post transfection by flow-cytometer. (E, F) mAb titer was measured 7 days post transfection by ForteBio Octet.

  • Development and physiological characterization of cellobiose-consuming Yarrowia lipolytica

    Development and physiological characterization of cellobiose‐consuming Yarrowia lipolytica

    Profiles of sugar consumption (solid symbols) and biomass accumulation (hollow symbols) of cells cultured in excess nitrogen media with cellobiose (top) and glucose (bottom). Cultures were inoculated to an initial cell density equal to an OD of 0.05 and incubated at 30 °C and 250 rpm. The strains profiled are Po1f-pINT03 (square), Po1f-B (triangle), and Po1f-BC (circle). Results are the mean of duplicate experiments. Error bars indicate standard deviations and are not visible when smaller than the symbol size. Consumption rates displayed in Table III are calculated from the 43 h data point.

  • An ultra scale-down analysis of the recovery by dead-end centrifugation of human cells for therapy

    An ultra scale‐down analysis of the recovery by dead‐end centrifugation of human cells for therapy

    Recovery by centrifugation of P4E6 cells as a function of cell concentration, cell hold time prior to centrifugation, RCF and spin time. DoE relationship values are reported as predicted percentage recovery of intact cells, REC (equation 1). The resultant model fits incorporating terms relating to all three operating variables and their possible combinations are of high significance level (P = 0.02 and P = 0.03 for 1 × 106 cells/mL and 2 × 106 cells/mL, respectively. Temperature 21°C, medium CGM. The area to the left and below the red dotted line (R < 250xg and S < 3 min) represents data extrapolated from the model created. The effect of increased hold time is an approximately linear proportional decrease in REC for the equivalent combinations of R and S (relationships not shown here).

  • A fast sensor for in vivo quantification of cytosolic phosphate in Saccharomyces cerevisiae

    A fast sensor for in vivo quantification of cytosolic phosphate in Saccharomyces cerevisiae

    Intracellular concentration of batch (shake flask) cultivation. Glucose, G6P, maltose (×10), and calculated cytosolic Pi concentrations (exponential phase) using four different carbon-sources: glucose, galactose, sucrose, ethanol. Error bars represent the standards deviation of three independent cultivations.

  • Excess surface area in bioelectrochemical systems causes ion transport limitations
  • Improved virus purification processes for vaccines and gene therapy
  • A simple and low‐cost platform technology for producing pexiganan antimicrobial peptide in E. coli
  • A high cell density transient transfection system for therapeutic protein expression based on a CHO GS‐knockout cell line: Process development and product quality assessment
  • Development and physiological characterization of cellobiose‐consuming Yarrowia lipolytica
  • An ultra scale‐down analysis of the recovery by dead‐end centrifugation of human cells for therapy
  • A fast sensor for in vivo quantification of cytosolic phosphate in Saccharomyces cerevisiae

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