Biotechnology and Bioengineering

Cover image for Vol. 112 Issue 3

Edited By: Douglas S. Clark

Impact Factor: 4.164

ISI Journal Citation Reports © Ranking: 2013: 26/165 (Biotechnology & Applied Microbiology)

Online ISSN: 1097-0290

Featured

  • Scaled-up manufacturing of recombinant antibodies produced by plant cells in a 200-L orbitally-shaken disposable bioreactor

    Scaled‐up manufacturing of recombinant antibodies produced by plant cells in a 200‐L orbitally‐shaken disposable bioreactor

    Upstream and downstream process steps for the production of M12 in BY-2 cells. Details of the process steps are indicated below the pictures.

  • Natural and engineered production of taxadiene with taxadiene synthase

    Natural and engineered production of taxadiene with taxadiene synthase

    Molecular structure of TXS and orientation of taxadiene in the active site of TXS. a: Structure of T. brevifolia TXS is represented by three domains, the C-terminus Class I terpenoid in blue and the N-terminus Class II terpenoids in green and yellow; (b) orientation of taxadiene (green) in the active site cavity exposing H5β atom of taxen-4-yl cation towards Y688; (c) orientation of taxadiene (blue) in the active site cavity exposing H5β atom of taxen-4-yl cation towards pyrophosphate PPi anion. Reprinted by permission from Macmillan Publishers Ltd: [Nature] (Koksal et al., ), copyright (2011).

  • Bioproduction of highly charged designer peptide surfactants via a chemically cleavable coiled-coil heteroconcatemer

    Bioproduction of highly charged designer peptide surfactants via a chemically cleavable coiled‐coil heteroconcatemer

    Time course of Het2-6 cleavage. Separate panels show species present at A) high, B) intermediate and C) low concentrations. Symbols indicate species concentrations determined by LCMS, while solid lines indicate data from the model fit.

  • Simple amino acid tags improve both expression and secretion of Candida antarctica lipase B in recombinant Escherichia coli

    Simple amino acid tags improve both expression and secretion of Candida antarctica lipase B in recombinant Escherichia coli

    Crystal structure of D5-CalB. (A) Stereo ribbon diagrams of the dimers of CalB and D5-CalB. Two monomers of CalB and D5-CalB are colored differently (salmon and light blue, respectively). Polyethylene glycol molecules bound in the middle of D5-CalB are shown as a stick model (colored in magenta). To compare relative positions of CalB and D5-CalB monomers, residues Pro293-Ala312 of CalB and D5-CalB are colored in green, respectively. All figures were produced using PyMOL (http://www.pymol.org). (B and C) Molecular interactions in the dimer interface of D5-CalB shown in detail. Two monomers are colored as in (A). Residues mutated in this study are shown in stick models.

  • Enhanced production of fatty alcohols by engineering the TAGs synthesis pathway in Saccharomyces cerevisiae

    Enhanced production of fatty alcohols by engineering the TAGs synthesis pathway in Saccharomyces cerevisiae

    Intracellular and extracellular fatty alcohol production levels in Δdga1-FAR strain grown in culture conditions with different carbon and nitrogen concentration. C2%-N2.5 g/L: culture condition with 2% galactose (wt/vol) and 2.5 g/L ammonium sulfate; C2%-N0.1 g/L: 2% galactose (wt/vol) and 0.1 g/L ammonium sulfate; C6%-N2.5 g/L: 6% galactose (wt/vol) and 2.5 g/L ammonium sulfate; C6%-N0.1 g/L: 6% galactose (wt/vol) and 0.1 g/L ammonium sulfate. The standard deviation was calculated from three independent experimental results.

  • Multiple approaches to predicting oxygen and glucose consumptions by HepG2 cells on porous scaffolds in an axial-flow bioreactor

    Multiple approaches to predicting oxygen and glucose consumptions by HepG2 cells on porous scaffolds in an axial‐flow bioreactor

    CFD simulation profiles in the 0.2 cm thick scaffold region from the central axis showing magnitude at a flow rate of 1 mL/min: (a) velocity, (b) shear stress, and (c) deformation. Below each profile is the color scale bar. Arrow indicates flow direction.

  • Non-destructive label-free continuous monitoring of in vitro chondrogenesis via electrical conductivity and its anisotropy

    Non‐destructive label‐free continuous monitoring of in vitro chondrogenesis via electrical conductivity and its anisotropy

    Histological appearance of disc-type cartilages during the in vitro chondrogenesis process of 6 weeks. We cultured the cartilage tissues using a chondrogenic media containing 0.3 mM vitamin C. At each time point, we fixed each sample and stained it with H&E, Safranin O, Toluidine blue, and trichrome for histological features, GAGs production, and collagenous matrix production.

  • Scaled‐up manufacturing of recombinant antibodies produced by plant cells in a 200‐L orbitally‐shaken disposable bioreactor
  • Natural and engineered production of taxadiene with taxadiene synthase
  • Bioproduction of highly charged designer peptide surfactants via a chemically cleavable coiled‐coil heteroconcatemer
  • Simple amino acid tags improve both expression and secretion of Candida antarctica lipase B in recombinant Escherichia coli
  • Enhanced production of fatty alcohols by engineering the TAGs synthesis pathway in Saccharomyces cerevisiae
  • Multiple approaches to predicting oxygen and glucose consumptions by HepG2 cells on porous scaffolds in an axial‐flow bioreactor
  • Non‐destructive label‐free continuous monitoring of in vitro chondrogenesis via electrical conductivity and its anisotropy

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