Genetic engineering of CHO cells for viral resistance to minute virus of mice
Joaquina X. Mascarenhas, Nikolay Korokhov, Lisa Burger, Ademola Kassim, Jason Tuter, Daniel Miller, Trissa Borgschulte, Henry J. George, Audrey Chang, David J. Pintel, David Onions and Kevin J. Kayser
Version of Record online: 12 OCT 2016 | DOI: 10.1002/bit.26186
Cell engineering to reduce cell surface sialic acid by affecting major glycosylation pathways. Complete absence of cell surface sialic acid in the Slc35a1 knockout cell line leads to absolute resistance to MVM infection. Our data demonstrate that resistance against MVM virus can be incorporated into CHO production cell lines, adding another level of defense against the devastating financial consequences of this virus infection while maintaining optimum recombinant protein yield and quality.