Microfluidic perfusion culture of human induced pluripotent stem cells under fully defined culture conditions
Ryosuke Yoshimitsu, Koji Hattori, Shinji Sugiura, Yuki Kondo, Rotaro Yamada, Saoko Tachikawa, Taku Satoh, Akira Kurisaki, Kiyoshi Ohnuma, Makoto Asashima and Toshiyuki Kanamori
Article first published online: 30 NOV 2013 | DOI: 10.1002/bit.25150
Human iPS cells (hiPSCs) are a promising source for drug discovery, although there are two potential problems: 1) instability of culture conditions caused by conventional culture plates, and 2) distortion of screening results due to undefined culture conditions. Yoshimitsu and coworkers have demonstrated that undifferentiated and differentiated states and the proliferation of hiPSCs can be controlled by a microfluidic perfusion system with fully defined culture conditions. Their system will be a platform technology for future large-scale screenings using hiPSCs.