Biotechnology and Bioengineering

Cover image for Vol. 111 Issue 9

Early View (Online Version of Record published before inclusion in an issue)

Edited By: Douglas S. Clark

Impact Factor: 4.164

ISI Journal Citation Reports © Ranking: 2013: 26/165 (Biotechnology & Applied Microbiology)

Online ISSN: 1097-0290


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  1. Articles

    1. Effect of granular activated carbon concentration on the content of organic matter and salt, influencing E. coli activity and survival in fluidized bed disinfection reactor

      Justina Racyte, Alette A.M. Langenhoff, Ana F.M.M.R. Ribeiro, Astrid H. Paulitsch-Fuchs, Harry Bruning and Huub H.M. Rijnaarts

      Article first published online: 16 JUN 2014 | DOI: 10.1002/bit.25254

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      Physical–chemical changes are caused in microbial media by different concentrations of granular activated carbon (GAC). These physical–chemical changes have an effect on the metabolic activity and survival of E. coli in a fluidized bed system. With increasing GAC concentration in the media, the adsorption of organic, and mineral compounds was increasing, resulting in decay of E.coli metabolic activity and survival in all tested media.

  2. Communication to the Editors

    1. Monocyte-based microrobot with chemotactic motility for tumor theragnosis

      Sung Jun Park, Yeonkyung Lee, Young Jin Choi, Sunghoon Cho, Han-Earl Jung, Shaohui Zheng, Bang Ju Park, Seong Young Ko, Jong-Oh Park and Sukho Park

      Article first published online: 9 JUN 2014 | DOI: 10.1002/bit.25270

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      Validation of tumor targeting ability of a biomedical microrobot using monocyte-based microrobot and modified cell migration chamber. (A) Development of monocyte-based microrobot using various sized fluorescent PS microbeads and monocytes. (B) Schematic representation of cell migration chamber and (C) experimental setup for validation of transmigrating ability of monocyte-based microrobot.

    2. An improved dynamic method to measure kLa in bioreactors

      Andrew L. Damiani, Min Hea Kim and Jin Wang

      Article first published online: 9 JUN 2014 | DOI: 10.1002/bit.25258

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      In this work, an improved dynamic method was developed to measure kLa. Compared to the classical dynamic method, the improved method incorporates mass transfer between the headspace and the liquid phase to improve the model accuracy. In addition, the procedure was adjusted by feeding in nitrogen gas during oxygen shut-off phase, which enables a faster and more accurate measurement of kLa. The effectiveness of the improved method was demonstrated using Scheffersomyces Stipitis.

    3. Exoelectrogenic biofilm as a template for sustainable formation of a catalytic mesoporous structure

      Matthew D. Yates, Roland D. Cusick, Ivan Ivanov and Bruce E. Logan

      Article first published online: 4 JUN 2014 | DOI: 10.1002/bit.25267

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      Actively respiring biofilms of Geobacter sulfurreducens were used as a biotemplate to form a palladium mesoporous layer directly on an electrode surface. Cells and proteins within the biofilm acted as the reductant and stabilizer to facilitate the reduction, dispersion, and attachment of palladium nanoparticles to the electrode surface without using synthetic chemicals.

  3. Articles

    1. You have full text access to this OnlineOpen article
      Integration of host strain bioengineering and bioprocess development using ultra-scale down studies to select the optimum combination: An antibody fragment primary recovery case study

      Jean P. Aucamp, Richard Davies, Damien Hallet, Amanda Weiss and Nigel J. Titchener-Hooker

      Article first published online: 4 JUN 2014 | DOI: 10.1002/bit.25259

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      An ultra scale-down (USD) process sequences was implemented to rank genetically engineered E. coli strains based on their ability to withstand cellular damage induced by the primary recovery steps of a pilot scale bioprocess. Harvested cell material with different genetic modifications obtained by various fermentation strategies displayed significant differences in withstanding discharge induced damage during continuous centrifugation. Here, the authors reported a screening methodology to assess the suitability of novel strains, generated during early-phase development, for use in bioprocesses.

    2. A cleavable silica-binding affinity tag for rapid and inexpensive protein purification

      Brandon L. Coyle and François Baneyx

      Article first published online: 4 JUN 2014 | DOI: 10.1002/bit.25257

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      We describe a new affinity tag suitable for single-step and rapid protein purification on inexpensive silica gel. A companion approach for cost-effective tag excision is also described.

    3. The role of pH on the resistance of resting- and active anammox bacteria to NO2 inhibition

      José M. Carvajal-Arroyo, Daniel Puyol, Guangbin Li, Reyes Sierra-Álvarez and Jim A. Field

      Article first published online: 4 JUN 2014 | DOI: 10.1002/bit.25269

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      The pH plays a critical role in the resistance of the anammox bacteria to NO2 inhibition. The NO2 tolerance of resting cells was more influenced by the pH than actively metabolizing cells. Exposure to NO2 at pH values less than 7.2 resulted in enhanced inhibition, increased emissions of NO gas and triggered a temporary increase in the ATP content of the cells in response to stress.

    4. A new method for generating high purity motoneurons from mouse embryonic stem cells

      Dylan A. McCreedy, Chelsea R. Brown, Jessica C. Butts, Hao Xu, James E. Huettner and Shelly E. Sakiyama-Elbert

      Article first published online: 4 JUN 2014 | DOI: 10.1002/bit.25260

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      Long term motoneuron cultures can be difficult to perform due to uncharacterized interactions with contaminating cell types. We demonstrate high purity motoneuron cultures using a genetically engineered mouse embryonic stem cell line with cells differentiating into mature motoneurons that demonstrate appropriate cellular and electrophysiological properties. This work establishes a platform that can be used in future studies focused on motoneuron development and disease.

    5. NADPH-dependent reductive biotransformation with Escherichia coli and its pfkA deletion mutant: Influence on global gene expression and role of oxygen supply

      Solvej Siedler, Stephanie Bringer, Tino Polen and Michael Bott

      Article first published online: 4 JUN 2014 | DOI: 10.1002/bit.25271

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      Reduction of methyl acetoacetate to methyl hydroxybutyrate served as a NADPH-dependent model reaction to study cofactor regeneration via the metabolism of the cell. Partial cyclization of the pentose phosphate pathway was accomplished by deletion of phosphofructokinase (pfkA). The different abilities to use oxygen effectively of the reference strain (BL21(DE3)) and its ΔpfkA mutant are discussed, as well as the role of the soluble transhydrogenase in the deletion strain.

  4. Communication to the Editors

    1. NADH plays the vital role for chiral pure D-(−)-2,3-butanediol production in Bacillus subtilis under limited oxygen conditions

      Jing Fu, Zhiwen Wang, Tao Chen, Weixi Liu, Ting Shi, Guanglu Wang, Ya-jie Tang and Xueming Zhao

      Article first published online: 28 MAY 2014 | DOI: 10.1002/bit.25265

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      Unlike previous reports in which two stereoisomers (with a ratio of 3:2) were produced, the authors first found that wild type B. subtilis 168 generated only D-(−)-2,3-butanediol (purity >99%) under low oxygen conditions. It was indicated that the high level of NADH availability, instead of high acetoin reductase activity, contributed more to 2,3-butanediol production in B. subtilis. Finally, BSF20 produced 49.29 g/L D(−)-2,3-butanediol, demonstrating B. subtilis is a competitive producer for chiral 2,3-butanediol production.

  5. Articles

    1. A versatile approach to the generation of fluid supported lipid bilayers and its applications

      Samaneh Mashaghi and Antoine M. van Oijen

      Article first published online: 28 MAY 2014 | DOI: 10.1002/bit.25273

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      Towards synthetic membrane biology: A generic and facile approach to the production of fluid supported lipid bilayers, which allows the incorporation of any lipids, including cholesterol, and transmembrane proteins are proposed. Several applications, including the incorporation and single-molecule tracking of transmembrane proteins into a bilayer and the visualization of the fusion of individual, membrane-enveloped viruses with a supported membrane, are demonstrated.

    2. Addition of citrate to Acidithiobacillus ferrooxidans cultures enables precipitate-free growth at elevated pH and reduces ferric inhibition

      Xiaozheng Li, Roel Mercado, Timothy Kernan, Alan C. West and Scott Banta

      Article first published online: 28 MAY 2014 | DOI: 10.1002/bit.25268

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      Growths kinetics and maximum cell yield of Acidithiobacillus ferrooxidans increase with increasing pH in precipitate-free cultures. Addition of citrate enables precipitate-free cultures with high iron concentration at high pH. Highest cell yield is obtained at pH 2.2 with 72 mM Fe(II) plus 70 mM citrate.

    3. Identification of manipulated variables for a glycosylation control strategy

      Melissa M. St. Amand, Devesh Radhakrishnan, Anne S. Robinson and Babatunde A. Ogunnaike

      Article first published online: 22 MAY 2014 | DOI: 10.1002/bit.25251

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      St. Amand and coworkers studied the effect of media supplements on the glycan distribution. They implemented a statistically designed series of experiments to determine the significant main effects (as well as interaction effects) of media supplementation with manganese, galactose and ammonia. The authors' findings indicate that each had significant effects on certain glycans: 1) MnCl2 can be used as a manipulated variable to increase the relative abundance of M51 and decrease FA2 simultaneously, and 2) galactose can be used as a manipulated variable to increase the relative abundance of FA2G1 and decrease FA2 and A2 simultaneously.

    4. Regeneration and control of human fibroblast cell density by intermittently delivered pulsed electric fields

      Alexander Golberg, Marianna Bei, Robert L. Sheridan and Martin L. Yarmush

      Article first published online: 22 FEB 2013 | DOI: 10.1002/bit.24832

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      Intermittently delivered pulsed electric fields for cell density control. The top line shows the timing for pulses delivery. The line on the bottom shows the respective cell concentration in the culture. E0- Electric field in applied on the cells population between the treatments, Eep- Electric field applied on cell population during the treatment, Tp- Time interval between the treatment, Tep- Total time of the delivery of electric field, Chigh- Higher threshold of cell density, Clow- Lower threshold for cell density.


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