Biotechnology and Bioengineering

Cover image for Vol. 114 Issue 6

Edited By: Douglas S. Clark

Impact Factor: 4.243

ISI Journal Citation Reports © Ranking: 2015: 24/161 (Biotechnology & Applied Microbiology)

Online ISSN: 1097-0290

Author Guidelines

NIH Public Access Mandate
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Author Guidelines

Types of Manuscripts

Articles should not exceed 15 double-spaced pages of text (not including references) and should contain no more than 10 figures and/or tables.

Communications to the Editor should not exceed 8 double-spaced pages of text (not including references) and should contain no more than 20 references and 4 figures and/or tables.

Regarding serial or companion papers, which must be reviewed together, no more than two manuscripts may be submitted at the same time.

Reviews and Mini-Reviews should be concise and critical assessments of the recent literature on a fast-moving area of contemporary interest. Reviews should not exceed 22 double-spaced pages of text (not including references) and should contain no more than 15 figures and/or tables. Mini-Reviews should not exceed 8 double-spaced pages of text, and should not contain more than 20 references and 4 figures and/or tables. Authors must contact the Board of Review Editors at on the suitability of a subject and the qualifications of the authors before submitting a Review or Mini-Review.

Exceptions to the lengths of the various types of manuscripts will be considered on an individual basis upon request by the authors. The authors must explain clearly, upon manuscript submission, the reasons for the longer manuscript.

Commentaries provide a forum for communication of opinion, insight, and interpretation on a recently published B&B paper and are submitted at the request of our editors.

Perspectives are submitted at the request of the Editor-in-Chief.

Manuscript Submission

The journal to which you are submitting your manuscript employs a plagiarism detection system. By submitting your manuscript to this journal you accept that your manuscript may be screened for plagiarism against previously published works.

Biotechnology and Bioengineering is a member of, and subscribes to the principles of, the Committee on Publication Ethics (COPE) (

All manuscripts must be submitted online at . Specific requirements for manuscript submission are detailed at the ScholarOne Manuscripts website on the “Submit a Manuscript” page. The following items are also required as part of the manuscript submission process:

•A cover letter containing a brief statement regarding the important contributions of the submitted paper and the reason(s) it should be published in Biotechnology and Bioengineering

•The subject category best suited for the manuscript content

•The names, addresses, phone numbers and e-mail addresses of four or five potential independent reviewers

•Image and text for graphical Table of Contents

The suggested reviewers should not be affiliated with the institution of the authors. Please note that the list of reviewers should not include the Editor-in-Chief or any of the Associate Editors of Biotechnology and Bioengineering . The authors may request that certain reviewers be excluded from reviewing their paper if they feel that these reviewers may be unfavorably biased.

Biotechnology and Bioengineering is initiating publication of a graphical Table of Contents. Please supply an image and text for this purpose that adheres to the following guidelines:

Image: Colorful, mostly square images are optimal. Please make sure images are of high enough quality for online publication.

Text: Descriptive text (2-3 sentences; 80 word maximum) which should capture the most compelling aspects of the work, aimed at a broad readership.

Conflict of Interest Disclosure: Biotechnology and Bioengineering requires that all authors disclose any potential sources of conflict of interest. Any interest or relationship, financial or otherwise, that might be perceived as influencing an author’s objectivity is considered a potential source of conflict of interest. These must be disclosed when directly relevant or indirectly related to the work that the authors describe in their manuscript. Potential sources of conflict of interest include but are not limited to patent or stock ownership, membership of a company board of directors, membership of an advisory board or committee for a company, and consultancy for or receipt of speaker’s fees from a company. The existence of a conflict of interest does not preclude publication in this journal.

If the authors have no conflict of interest to declare, they must also state this at submission. It is the responsibility of the corresponding author to review this policy with all authors and to collectively list in the cover letter to the Editor-in-Chief, in the manuscript (in the Acknowledgments section), and in the online submission system ALL pertinent commercial and other relationships.

Questions regarding manuscript submission should be directed to the Editorial Office at or (201) 748-6232.

Manuscript Preparation

Prepare your text in Word, Post Script or Rich Text Format, using American spelling and grammar. We also accept manuscripts created with LaTeX.

Do not embed figures or tables in the document. The text and references should be double-spaced with one inch margins. Use Times New Roman size 12 font or equivalent.

All manuscripts should have a separate title page with the title of the paper, the names and affiliations of all authors, the name, address, phone number and e-mail address (and FAX number if available) of the corresponding author, and a short running title of no more than 45 characters.

Articles should contain an Abstract, Introduction, Materials and Methods (including Computational Methods), Modeling or Theoretical Aspects (optional), Results, Discussion (can be combined with Results), Conclusions (optional), Acknowledgments (optional), Nomenclature (optional), and References.

Communications to the Editor should not be divided into sections except for Materials and Methods (including Computational Methods). A short Abstract (preferably less than 200 words), a brief introduction (not divided into its own section), Acknowledgments (optional), and References should be included. Communications to the Editor are subjected to the same review process as Articles, and they should not constitute preliminary investigations.

Reviews and Mini-Reviews should contain an Abstract, a brief Introduction, appropriate subsections for various topics, Acknowledgments, and References.

All types of manuscripts begin with a title page consisting of the title of the manuscript, author affiliation, and the corresponding author information. Page 2 of the manuscript should consist of an Abstract followed by Keywords (3–6 key words or phrases). The body of the manuscript should begin on page 3. For all types of manuscripts, the list of References should be followed by the Tables, the List of Figures with their legends (separate from the images), and finally the numbered copies of the Figures. Acknowledgments of personal or financial assistance should be made in a separate paragraph following the Discussion or Conclusions sections.

Supplementary Online Material: Authors are invited to submit supplementary material for their articles. Supplementary material may include (but is not limited to) video clips, analytical and spectral characterization data, crystallographic information files, large sections of tabular data, derivations, program code, or graphical files that are not suitable for print media. Supplementary material for published articles will be made available via a link in the online version of the article.

The title should be specific, informative and as short as possible. For Articles, the main text of the paper should be reasonably divided into subsections as necessary by inserting appropriate headings. Please refer to any issue of B&B for examples. Do not use footnotes in the text. The Erratum section allows authors to correct inadvertent (e.g. typographical) errors in published papers. Changes or additions to the manuscript and its data are not permitted. Errata should be submitted directly to the publisher.


The list of References should include all relevant and necessary published work, and all listed references must be cited in the text. The reference list should be arranged in alphabetical order by first author. Do not use numbers. In the text, references should be cited by the author's surname with year of publication in parentheses. For more than two authors, e.g., Briggs, Porter, and King (1993), the reference citation in the text would be Briggs et al. (1993). If there is more than one citation in the same place, put the different citations in parentheses, in alphabetical order, and separated by a semicolon, e.g., (Adam, 1995; Dorow and O'Neal, 1974). Journal titles should be abbreviated according to the Chemical Abstracts Service Source Index.

The listed references should include only published journal papers, books, book chapters, and Ph.D. theses and dissertations. Manuscripts in preparation or submitted, abstracts, meeting presentations, editorials, patents, M.S. theses, letters to the editor, published science briefings, and in general all other published material that has not been edited or refereed should not be listed in the References. Such material can be mentioned parenthetically in the text. If an "in press" publication is included in the list of References, a copy must be submitted with the manuscript as Supplementary Material so that this reference can become available to the reviewers. For reference citations, follow the styles of the examples below:

Journal article

King VM, Armstrong DM, Apps R, Trott JR. 1998. Numerical aspects of pontine, lateral reticular, and inferior olivary projections to two paravermal cortical zones of the cat cerebellum. J Comp Neurol 390:537-551.


Voet D, Voet JG. 1990. Biochemistry. New York:John Wiley & Sons. 1223 p.

Book chapter

Gilmor ML, Rouse ST, Heilman CJ, Nash NR, Levey AI. 1998. Receptor fusion proteins and analysis. In: Ariano MA, editor. Receptor localization. New York: Wiley-Liss. p 75-90.


Tables should be numbered with capital Roman numerals in the order they appear in the text. All table columns should have an explanatory heading. Duplication of information contained in tables and figures should be avoided as much as possible. Long tables containing a large amount of information can be avoided by showing only typical or the most important results.

Prepare your tables in Word, Post Script or Rich Text Format. Do not embed tables in the text portion of the manuscript.

Figures and Illustrations

Number figures sequentially using Arabic numbers. All figures must be accompanied by a figure legend. The list of figure legends should follow the Tables section.

To ensure the highest print quality, your figures must be submitted in .TIF format according to the following minimum resolutions:

•300 dpi (dots per inch) for black and white line art (simple bar graphs, charts, etc.)

•300 dpi for halftones (black and white photographs)

•300 dpi for combination halftones (photographs that also contain line art such as labeling or thin lines)

Vector-based figures (e.g. figures created in Adobe Illustrator) should be submitted in .EPS format. Color figures must be submitted in a CMYK colorspace. Do not submit color figures as RGB.

For best reproduction, bright, clear colors should be used. Dark colors against a dark background do not reproduce well; please place your color images against a white background wherever possible. Please contact the production editor ( for further information.

Symbols and Nomenclature

The nomenclature sponsored by the International Union of Pure and Applied Chemistry is requested for chemical compounds. All necessary quantities should be defined with units compatible with the SI system. If many symbols are used in the text and in equations it is advisable to assemble the definitions and units of all symbols in a Nomenclature section just before the list of References. Symbols should be assembled alphabetically.


If your paper is accepted, the author identified as the formal corresponding author for the paper will receive an email prompting them to login into Author Services; where via the Wiley Author Licensing Service (WALS) they will be able to complete the license agreement on behalf of all authors on the paper.
For authors signing the copyright transfer agreement:
If the OnlineOpen option is not selected the corresponding author will be presented with the copyright transfer agreement (CTA) to sign. The terms and conditions of the CTA can be previewed in the samples associated with the Copyright FAQs below:
CTA Terms and Conditions
For authors choosing OnlineOpen:
If the OnlineOpen option is selected the corresponding author will have a choice of the following Creative Commons License Open Access Agreements (OAA):
Creative Commons Attribution License OAA
Creative Commons Attribution Non-Commercial License OAA
Creative Commons Attribution Non-Commercial -NoDerivs License OAA
To preview the terms and conditions of these open access agreements please visit the Copyright FAQs hosted on Wiley Author Services and visit
If you select the OnlineOpen option and your research is funded by The Wellcome Trust and members of the Research Councils UK (RCUK) you will be given the opportunity to publish your article under a CC-BY license supporting you in complying with Wellcome Trust and Research Councils UK requirements. For more information on this policy and the Journal’s compliant self-archiving policy please visit:

Editorial Policy

All authors take public responsibility for the accuracy, reproducibility and veracity of the content of their submitted work. Manuscript submission implies all authors meet the criteria described below and that no authors meeting the criteria have been omitted. Authorship is defined by the journal as making a substantial contribution to the manuscript with respect to:

• Substantive intellectual contributions in conception, design or implementation of the work

• Interpretation and analysis of information or data

• Manuscript preparation including writing, critical revision and/or ultimate approval of the drafted manuscript for submission

Manuscripts submitted to B&B must represent original research or original review on subjects within the scope of the journal as outlined in the Aims and Scope section (which appears under the Overview link above). All authors must agree to its submission to B&B and to the implications of this submission as described below. All authors are responsible for the complete contents of their manuscript. By its submission to B&B, all the authors guarantee that the manuscript, in its present or a substantially similar form, has not been published or is not being considered for publication elsewhere. Publication in any reasonably retrievable source constitutes prior publication. Meeting abstracts do not constitute prior publication.

The number of authors on a single manuscript should not exceed 12. Manuscripts that contain more than 12 authors will be returned to the authors without review. Other contributors who do not meet the requirements of authorship may be listed in the Acknowledgments.

It is the authors' responsibility to obtain permissions for reproduction of figures, tables, or text from published work. The publisher of a journal or book is the copyright owner from whom the written permission must be obtained. Permission from authors is also encouraged as a professional courtesy.

Although Biotechnology and Bioengineering encourages international submissions, we require that authors employ American spelling and grammar. Authors who are not fluent in English are strongly encouraged to seek outside assistance with manuscript preparation. Manuscripts can be rejected or returned to the authors solely on the basis of substandard English.

Revised manuscripts must be returned within 6 weeks upon receiving notification with the reviews by an editor. Otherwise, they will be considered withdrawn and treated as new manuscripts upon resubmission.

Proofs will be sent to the corresponding author who must be identified on the title page. Page charges will not be made. Reprints may be ordered at All color figures will be reproduced in full color in the online edition of the journal at no cost to authors. Authors are requested to pay the cost of reproducing color figures in print; the fee is $500 per figure. Authors are encouraged to submit color illustrations that convey or clarify essential scientific information.

Cover Illustrations: If you would like to send suggestions for artwork related to your manuscript to be considered to appear on the journal cover, please follow these general guidelines.

Submission of Protein Structures: Authors are expected to deposit the coordinates for new protein structures in the Protein Data Bank ( and include the assigned identification codes in the manuscript.

Submission of Sequence Data: The Editors encourage the use of widely accessible genetics databases as repositories for newly determined nucleotide and/or amino acid sequence data. Authors should always include the appropriate database (e.g. GenBank) accession number(s) in the Materials and Methods section of the manuscript.

Deposition of Cultures: Authors are encouraged to deposit important strains in publicly accessible culture collections and include the collection and strains designations in the text of the manuscript.

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