Biotechnology and Bioengineering

Cover image for Vol. 112 Issue 2

Edited By: Douglas S. Clark

Impact Factor: 4.164

ISI Journal Citation Reports © Ranking: 2013: 26/165 (Biotechnology & Applied Microbiology)

Online ISSN: 1097-0290

Virtual Issue: Metabolic Engineering

Edited by Jens Nielsen

Metabolic engineering is the enabling science associated with the design and development of efficient cell factories that can be used for sustainable, bio-based production of fuels, chemicals and materials. Due to the substantial engineering component of metabolic engineering, B&B has been a natural home for many published papers on metabolic engineering since its emergence as a research field in the early 1990's.

In this virtual issue, B&B highlights several recent publications in the field of metabolic engineering. These include advances in metabolic modeling, a key component of cell factory design, novel molecular biology tools for rapid construction of novel cell factories and implementation of metabolic engineering strategies, i.e. the development and evaluation of cell factories that are producing valuable chemicals. In Song and Ramkrishna (2010) an approach is presented to improve the prediction from limited experimental data through the use of mathematical modeling and Brunk et al. (2011) presents a way to retrofit enzymes to specific biosynthetic pathways using computational methods. Among modeling tools there is also a paper by Colletti et al. (2011) that presents an evaluation of factors that influences overall yields based on statistical analysis of historical data.

In metabolic engineering there is a constant need for advancing our ability to rapidly implement genetic engineering strategies, and even though many advanced methods have been developed for Escherichia coli and Saccharomyces cerevisiae, two widely used cell factories, there is still need for new experimental techniques for these and other organisms. Several new interesting techniques are presented in this virtual issue. These include an accumulative site-specific integration system for CHO cells described by Kameyama et al. (2010), a set of broad-host range vectors for protein expression across different Gram negative bacteria described by Prior et al. (2010), and a genetic reporter system to evaluate cell proliferation rate of mammalian cells presented by Ryan et al. (2011).

The virtual issue also collects several papers on design and implementation of improved cell factories, including yeast improved for production of isoprenoids by Asadollahi et al. (2010) and Fischer et al. (2011), E. coli producing amino acids by Park et al. (2011) and Jung et al. (2010), 1,2-propanediol production by E. coli presented by Clomburg and Gonzalez (2011) and E. coli producing polyketides presented by Boghigian et al. (2011). In recent years adaptive evolution has also shown to be a powerful tool in metabolic engineering and two papers address this issue for improving sugar uptake rate, i.e. galactose by yeast by Lee et al. (2011) and xylose by Zymomonas mobilis by Agrawal et al. (2011).

Metabolic engineering of Escherichia coli for the production of polylactic acid and its copolymers
Yu Kyung Jung, Tae Yong Kim, Si Jae Park, and Sang Yup Lee

An accumulative site-specific gene integration system using cre recombinase-mediated cassette exchange
Yujiro Kameyama, Yoshinori Kawabe, Akira Ito, and Masamichi Kamihira

Enhancement of farnesyl diphosphate pool as direct precursor of sesquiterpenes through metabolic engineering of the mevalonate pathway in Saccharomyces cerevisiae
Mohammad A. Asadollahi, Jérôme Maury, Michel Schalk, Anthony Clark, and Jens Nielsen

Prediction of metabolic function from limited data: Lumped hybrid cybernetic modeling (L-HCM)
Hyun-Seob Song and Doraiswami Ramkrishna

Broad-host-range vectors for protein expression across gram negative hosts
Jamie E. Prior, Michael D. Lynch, and Ryan T. Gill

Improved galactose fermentation of Saccharomyces cerevisiae through inverse metabolic engineering
Ki-Sung Lee, Min-Eui Hong, Suk-Chae Jung, Suk-Jin Ha, Byung Jo Yu, Hyun Min Koo, Sung Min Park, Jin-Ho Seo, Dae-Hyuk Kweon, Jae Chan Park, and Yong-Su Jin

Adaptation yields a highly efficient xylose-fermenting Zymomonas mobilis strain
Manoj Agrawal, Zichao Mao, and Rachel Ruizhen Chen

Metabolic engineering of Escherichia coli for the production of 1,2-propanediol from glycerol
James M. Clomburg and Ramon Gonzalez

Evaluating factors that influence microbial synthesis yields by linear regression with numerical and ordinal variables
Peter F. Colletti, Yogesh Goyal, Arul M. Varman, Xueyang Feng, Bing Wu, and Yinjie J. Tang

Escherichia coli W as a new platform strain for the enhanced production of L-valine by systems metabolic engineering
Jin Hwan Park, Yu-Sin Jang, Jeong Wook Lee, and Sang Yup Lee

Multi-factorial engineering of heterologous polyketide production in Escherichia coli reveals complex pathway interactions
Brett A. Boghigian, Haoran Zhang, and Blaine A. Pfeifer

Metabolic engineering of monoterpene synthesis in yeast
Marc J. C. Fischer, Sophie Meyer, Patricia Claudel, Marc Bergdoll, and Francis Karst

A genetic reporter system to gauge cell proliferation rate
Ryan W.S. Peacock and Clifford L. Wang

Integrating computational methods to retrofit enzymes to synthetic pathways
Elizabeth Brunk, Marilisa Neri, Ivano Tavernelli, Vassily Hatzimanikatis, and Ursula Rothlisberger