Journal of Cellular Biochemistry

Cover image for Vol. 119 Issue 1

Early View (Online Version of Record published before inclusion in an issue)

Edited By: C. Fred Fox, Gary S. Stein, and Max M. Burger

Impact Factor: 3.085

ISI Journal Citation Reports © Ranking: 2016: 103/190 (Cell Biology); 131/290 (Biochemistry & Molecular Biology)

Online ISSN: 1097-4644

VIEW

  1. 1 - 100
  2. 101 - 145
  1. RESEARCH ARTICLES

    1. Assessment of chemotherapy on various biochemical markers in breast cancer patients

      Márcia F. C. J. Paz, Antonio L. Gomes Júnior, Muhammad T. Islam, Shams Tabrez, Nasimudeen R. Jabir, Mohammad Z. Alam, Kátia C. Machado, Marcus V. O. B. de Alencar, Keylla C. Machado, Eunus S. Ali, Siddhartha K. Mishra, Leonardo F. Gomes, André Luiz Pinho Sobral, João M. C. e Sousa, Geane F. de Souza, Ana Amélia C. Melo-Cavalcante and Juliana da Silva

      Version of Record online: 4 DEC 2017 | DOI: 10.1002/jcb.26487

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      Breast cancer and co-treatment with CYP and DOX, interfere various biological markers, thereby, showing the physiological imbalance.

    2. XIST promotes gastric cancer (GC) progression through TGF-β1 via targeting miR-185

      Quan Zhang, Baiyu Chen, Ping Liu and Jing Yang

      Version of Record online: 4 DEC 2017 | DOI: 10.1002/jcb.26447

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      We implied that XIST can be recognized as an oncogene in GC development.We revealed that XIST can act as a ceRNA by regulating TGF-β1 via sponging miR-185 in GC cells. All these implied that XIST/ miR-185/ TGF-β1 axis was involved in GC progression.

    3. You have full text access to this OnlineOpen article
      The advanced glycation end product Nϵ-carboxymethyllysine and its precursor glyoxal increase serotonin release from Caco-2 cells

      Ann-Katrin Holik, Barbara Lieder, Nicole Kretschy, Mark M. Somoza, Jakob P. Ley, Joachim Hans and Veronika Somoza

      Version of Record online: 4 DEC 2017 | DOI: 10.1002/jcb.26439

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      Nepsilon-Carboxymethyllysine (CML), which represents a well-characterized Maillard reaction product that is frequently encountered in a Western diet, and its precursor glyoxal induce serotonin release in fully differentiated Caco-2 cells. Whether the CML-/glyoxal-induced serotonin release plays a role in serotonin-mediated gastric motility has to be elucidated in future studies.

    4. Mesoderm-specific transcript localization in the ER and ER-lipid droplet interface supports a role in adipocyte hypertrophy

      Igor Prudovsky, Rea P. Anunciado-Koza, Chester G. Jacobs, Doreen Kacer, Matthew E. Siviski and Robert A. Koza

      Version of Record online: 4 DEC 2017 | DOI: 10.1002/jcb.26429

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      Mesoderm-specific transcript (Mest) is an imprinted gene that shows a broad range of expression in adipose tissue among genetically identical mice which is positively associated with dietary fat-induced adipose tissue expansion. Furthermore, inactivation of Mest in adipose tissue of mice results in reduced susceptibility for diet-induced obesity and suggests that MEST could function to facilitate lipid storage in adipocytes. Co-localization of endoplasmic reticulum containing MEST with the lipid droplet surface marker perilipin provides further support that MEST augments triacylglycerol storage in lipid droplets of cells undergoing adipogenesis.

    5. Molecular modeling studies to explore the binding affinity of virtually screened inhibitor toward different aminoglycoside kinases from diverse MDR strains

      Rishikesh S. Parulekar and Kailas D. Sonawane

      Version of Record online: 4 DEC 2017 | DOI: 10.1002/jcb.26435

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      Identification of most stable inhibitor ZINC71575479 of aminoglycoside kinase by structure based virtual screening technique and studying its binding affinity in comparison to tyrphostin AG1478 toward aminoglycoside kinases from different multidrug resistant strains by implementing molecular docking, molecular dynamics simulation, and binding free energy calculation techniques.

    6. You have full text access to this OnlineOpen article
      The potential of microRNAs as human prostate cancer biomarkers: A meta-analysis of related studies

      Chun-Jiao Song, Huan Chen, Li-Zhong Chen, Guo-Mei Ru, Jian-Jun Guo and Qian-Nan Ding

      Version of Record online: 4 DEC 2017 | DOI: 10.1002/jcb.26445

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      miRNAs have been shown to play an important role to predict prognosis of PC. The purpose of this meta-analysis is to integrate the currently published expression profile data of miRNAs in PC, and evaluate the value of miRNAs as biomarkers for PC.

    7. Inducing indel mutation in the SOX6 gene by zinc finger nuclease for gamma reactivation: An approach towards gene therapy of beta thalassemia

      Mehran Modares Sadeghi, Laleh Shariati, Zahra Hejazi, Mansoureh Shahbazi, Mohammad Amin Tabatabaiefar and Hossein Khanahmad

      Version of Record online: 30 NOV 2017 | DOI: 10.1002/jcb.26412

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      Using the ZFN technology, indel in SOX6 gene was induced. The gamma globin gene was overexpressed. We were able to increase the amount of HbF by manipulating the SOX6 gene.

    8. Unrestricted somatic stem cells, as a novel feeder layer: Ex vivo culture of human limbal stem cells

      Ebrahim Shirzadeh, Saeed Heidari Keshel, Vahid Ezzatizadeh, Sayena Jabbehdari and Alireza Baradaran-Rafii

      Version of Record online: 30 NOV 2017 | DOI: 10.1002/jcb.26434

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      Ex vivo culture of limbal stem cells (LSCs) is a current promising approach for reconstruction of the ocular surface. In this context, 3T3 feeder layer cells (mouse embryo fibroblast) are generally utilized to maintain and expand LSCs. The aim of this study is to develop a novel culture method to expand LSCs, using umbilical cord derived human unrestricted somatic stem cells (hUSSCs).

    9. Effects of apelin on retinal microglial cells in a rat model of oxygen-induced retinopathy of prematurity

      Wei Zhang, Ding-Guo Zhang, Xing Liang, Wei-Liang Zhang and Jing-Xue Ma

      Version of Record online: 28 NOV 2017 | DOI: 10.1002/jcb.26473

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      Appropriate concentration of apelin may reduce retinal microglial cells in a rat model of oxygen-induced ROP.

    10. Aspartate-β-semialdeyhyde dehydrogenase as a potential therapeutic target of Mycobacterium tuberculosis H37Rv: Evidence from in silico elementary mode analysis of biological network model

      Saif Khan, Pallavi Somvanshi, Tulika Bhardwaj, Raju K. Mandal, Sajad A. Dar, Mohd Wahid, Arshad Jawed, Mohtashim Lohani, Mahvish Khan, Mohammed Y. Areeshi and Shafiul Haque

      Version of Record online: 24 NOV 2017 | DOI: 10.1002/jcb.26458

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      Identification of potential therapeutic targets against Mycobacterium tuberculosis infections using bottom-up systems biology approach. We prioritized M. tuberculosis therapeutic targets based on flux and elementary mode analysis using direct mathematical modeling of the relevant metabolic pathways. Molecular docking and simulation studies of the priority target (ie, ASD) revealed the therapeutic potential of the selected natural product based ASD inhibitors. Our study highlights the crucial role of systems biology in conjunction with molecular interaction for probing novel leads against M. tuberculousis.

    11. The ICAM-1 expression level determines the susceptibility of human endothelial cells to simulated microgravity

      Ludmila B. Buravkova, Eugene G. Rudimov, Elena R. Andreeva and Anatoly I. Grigoriev

      Version of Record online: 24 NOV 2017 | DOI: 10.1002/jcb.26465

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      Non-activated ECs (low ICAM-1 expression) are quite resistant to simulated microgravity impact demonstrating even elevation of VE-cadherin gene and protein expression improving the integrity of endothelial monolayer. Pre-activation of ECs with inflammatory stimuli could hamper ECs adhesion profile attenuating its barrier function. These alterations may be among of the mechanisms underlying cardiovascular dysregulation in the real microgravity.

    12. Knockdown of long non-coding RNA ANRIL inhibits proliferation, migration, and invasion but promotes apoptosis of human glioma cells by upregulation of miR-34a

      Xuechao Dong, Zheng Jin, Yong Chen, Haiyang Xu, Chengyuan Ma, Xinyu Hong, Yunqian Li and Gang Zhao

      Version of Record online: 24 NOV 2017 | DOI: 10.1002/jcb.26437

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      ANRIL suppression inhibits growth, promotes apoptosis, and upregulates miR-34a. MiR-34a is a direct negative regulator of Sirt1 expression. Sirt1 activates the PI3K/AKT and mTOR signaling pathways.

    13. Structural insights into impact of Y134F mutation and discovery of novel fungicidal compounds against CYP51 in Puccinia triticina

      Bharati Pandey and Pradeep Sharma

      Version of Record online: 24 NOV 2017 | DOI: 10.1002/jcb.26422

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      RMSD, Root-mean-square deviation; RMSF, Root mean square fluctuation; Rg, Radius of gyration.

    14. Epiregulin (EREG) is upregulated through an IL-1β autocrine loop in Caco-2 epithelial cells with reduced CFTR function

      Macarena Massip-Copiz, Mariángeles Clauzure, Ángel G. Valdivieso and Tomás A. Santa-Coloma

      Version of Record online: 24 NOV 2017 | DOI: 10.1002/jcb.26483

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      CFTR signaling starts with changes in the intracelular Cl-concentration. When the CFTR activity is impaired, Cl-accumulates and starts an autocrine IL-1β positive feedback loop. Here, we show that epiregulin (EREG) mRNA and protein are over-expressed in Caco-2/pRS26 cells with impaired CFTR function, due to the increased IL-1β loop, EGFR activation and JNK signaling. Thus, EGFR and its ligands constitute new elements in the CFTR-signaling pathway.

    15. Long noncoding RNA HOTTIP promotes endothelial cell proliferation and migration via activation of the Wnt/β-catenin pathway

      Bihong Liao, Ruimian Chen, Feng Lin, Aihuan Mai, Jie Chen, Huimin Li, Zhenglei Xu and Shaohong Dong

      Version of Record online: 24 NOV 2017 | DOI: 10.1002/jcb.26448

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      (i) HOTTIP expression level was higher in coronary artery disease (CAD) tissues than in normal arterial tissues. (ii) Ectopic expression of HOTTIP promoted endothelial cell proliferation and also increased the expression of proliferating makers cyclin D1 and PCNA. (iii) Ectopic expression of HOTTIP increased endothelial cell proliferation and migration via activation of the Wnt/β-catenin pathway.

    16. Suppression of long non-coding RNA LET potentiates bone marrow-derived mesenchymal stem cells (BMSCs) proliferation by up-regulating TGF-β1

      Xin Jin, Zhiliang Zhang, Yi Lu and Zhihong Fan

      Version of Record online: 24 NOV 2017 | DOI: 10.1002/jcb.26459

      FGF-2 promotes BMSCs proliferation and down-regulates LET expression. Overexpression of LET inhibits BMSCs proliferation. Suppression of LET up-regulates the expression of TGF-β1 in BMSCs. TGF-β1 knockdown reverses sh-LET-induced BMSCs proliferation. TGF-β1 knockdown alleviates sh-LET-induced activation of Wnt/β-catenin pathway.

      Our study demonstrated that suppression of LET promotes BMSCs proliferation by up-regulating TGF-β1 and activating Wnt/β-catenin pathway. Our research may provide a new approach or prospect to promote BMSCs proliferation in vitro.

    17. Combination adjuvant chemotherapy with targeted drugs for treatment of colorectal cancer: A network meta-analysis

      Jinghui Wang, Li Luo, Dingxue Wang, Bin Guo, Jun Li, Zhu Yang and Dongxin Tang

      Version of Record online: 24 NOV 2017 | DOI: 10.1002/jcb.26312

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      The addition of targeted drugs will greatly improve the efficacy of chemotherapy generally. Targeted drug C plus chemotherapy regiment FOLFIRI is the preferable treatment for patients with CRC in clinical practice.

    18. Short-term hypoxia upregulated Mas receptor expression to repress the AT1R signaling pathway and attenuate Ang II-induced cardiomyocyte apoptosis

      Ruey-Lin Chang, Chih-Fen Chang, Da-Tong Ju, Tsung-Jung Ho, Tung-Ti Chang, Jing-Wei Lin, Jia-Chun Li, Shiu-Min Cheng, Cecilia-Hsuan Day, Vijaya Padma Viswanadha and Chih-Yang Huang

      Version of Record online: 24 NOV 2017 | DOI: 10.1002/jcb.26440

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      The proposed mechanism of STH-mediated MasR to repress the AT1R signaling pathway and attenuate Ang II-induced cardiomyocyte apoptosis.

    19. 4-bp insertion/deletion (rs3783553) polymorphism within the 3′UTR of IL1A contributes to the risk of prostate cancer in a sample of Iranian population

      Mohammad Hashemi, Gholamreza Bahari, Shamim Sarhadi, Ebrahim Eskandari, Behzad Narouie, Mohsen Taheri and Saeid Ghavami

      Version of Record online: 21 NOV 2017 | DOI: 10.1002/jcb.26427

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      We designed mismatched PCR-RFLP to evaluate the impact of 4-bp insertion/deletion polymorphism (rs3783553) within the 3′ untranslated region (3′UTRs) of IL1A on prostate cancer (PCa) risk in a sample of Iranian population. Our findings revealed an association between rs3783553 variant and risk of PCa. In addition the variant was associated with some clinicopathological characteristics of the patients, including stage, perineural invasion, and surgical margin. Bioinformatics analysis revealed that the ins/del variant affected the IL1A mRNA stability leading to a structural shift in IL1A mRNA and has-miR-125a-3p hybrid.

    20. Trichostatin A alters cytoskeleton and energy metabolism of pancreatic adenocarcinoma cells: An in depth proteomic study

      Elisa Dalla Pozza, Marcello Manfredi, Jessica Brandi, Arianna Buzzi, Eleonora Conte, Raffaella Pacchiana, Daniela Cecconi, Emilio Marengo and Massimo Donadelli

      Version of Record online: 21 NOV 2017 | DOI: 10.1002/jcb.26436

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      We performed an in depth shotgun proteomic study using the label-free proteomic SWATH-MS analysis to investigate novel insights of the mechanism of action of the histone deacetylase (HDAC) inhibitor trichostatin A (TSA) in PDAC cells. This proteomic analysis showed an overall up-regulation of cytokeratins and other proteins related to the cytoskeleton organization. On the contrary, a large amount of the down-regulated proteins by TSA treatment belongs to the cellular energetic metabolism and to the machinery of protein synthesis.

    21. c-Abl tyrosine kinase regulates neutrophil crawling behavior under fluid shear stress via Rac/PAK/LIMK/cofilin signaling axis

      Haibin Tong, Dake Qi, Xingang Guan, Guiquan Jiang, Zhiyong Liao, Xu Zhang, Peichao Chen, Nan Li and Mingjiang Wu

      Version of Record online: 20 NOV 2017 | DOI: 10.1002/jcb.26453

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      We demonstrate a critical signaling function of c-Abl kinase in neutrophil crawling under physiological shear by regulating Vav1 activity and Rac/PAK/LIMK/cofilin signaling axis. These findings provide the feasibility for targeting c-Abl kinase to restrict the recruitment of neutrophils for the amelioration of inflammation-related disease.

    22. You have full text access to this OnlineOpen article
      RUNX-mediated growth arrest and senescence are attenuated by diverse mechanisms in cells expressing RUNX1 fusion oncoproteins

      Gail Anderson, Nancy Mackay, Kathryn Gilroy, Jodie Hay, Gillian Borland, Alma McDonald, Margaret Bell, Siti Ayuni Hassanudin, Ewan Cameron, James C. Neil and Anna Kilbey

      Version of Record online: 20 NOV 2017 | DOI: 10.1002/jcb.26443

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      This study reveals the diverse mechanisms by which primary cells expressing RUNX1 fusion oncoproteins evade the negative growth consequences of over-expressed RUNX. Growth arrest activity is markedly attenuated in two fusion derivatives (TEL-RUNX1 and RUNX1-ETO9a) but not in RUNX1-ETO where it is instead antagonized by secretion of pro-oncogenic cytokines. These studies help to account for the markedly different biology and cell-type specificity of the RUNX1 oncoproteins.

    23. Intra-articular injection of a substance P inhibitor affects gene expression in a joint contracture model

      Mark E. Morrey, Joaquin Sanchez-Sotelo, Eric A. Lewallen, Kai-Nan An, Diane E. Grill, Scott P. Steinmann, Jie J. Yao, Christopher G. Salib, William H. Trousdale, Nicolas Reina, Hilal M. Kremers, David G. Lewallen, Andre J. van Wijnen and Matthew P. Abdel

      Version of Record online: 20 NOV 2017 | DOI: 10.1002/jcb.26256

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      Our manuscript reveals new data on the potential for in vivo delivery of the substance P antagonist fosaprepitant, as a possible strategy for the attenuation of post-traumatic joint contractures. Intra-articular injections were used to monitor the potential effects of this reagent on reducing joint contracture in an established rabbit model that mimics human arthrofibrosis. We highlight significant changes in mRNA expression over both acute and chronic phases of disease progression.

    24. GCNF regulates OCT4 expression through its interactions with nuclear receptor binding elements in NCCIT cells

      Sung-Won Park, Hyun-Jin Do, Wonbin Choi, Jin-Hoi Kim, Hyuk Song, Han Geuk Seo and Jae-Hwan Kim

      Version of Record online: 20 NOV 2017 | DOI: 10.1002/jcb.26438

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      OCT4 promoter activity was repressed by GCNF in a dose-dependent manner and three nuclear receptor binding sites are important for GCNF-induced OCT4 repression.

    25. Drug repurposing: An approach to tackle drug resistance in S. typhimurium

      Preethi Balasundaram, Shanthi Veerappapillai, Suthindhiran Krishnamurthy and Ramanathan Karuppasamy

      Version of Record online: 20 NOV 2017 | DOI: 10.1002/jcb.26457

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      About 1309 FDA-approved drugs molecules from Mantra 2.0 database were analysed for their activity against gyrase of S. typhimurium. Of note, our result is the first experimental evidence that reveals the flufenamic acid activity against Gram negative bacteria, S. typhimurium.

    26. BMP9/COX-2 axial mediates high phosphate-induced calcification in vascular smooth muscle cells via Wnt/β-catenin pathway

      Fang He, Han Wang, Wen-Yan Ren, Yan Ma, Yun-Peng Liao, Jia-Hui Zhu, Jin Cui, Zhong-Liang Deng, Yu-Xi Su, Hua Gan and Bai-Cheng He

      Version of Record online: 20 NOV 2017 | DOI: 10.1002/jcb.26460

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      BMP9 is involved in the phosphate-induced calcification in VSMCs. COX-2 may partly mediate BMP9-induced calcification in VSMCs through increasing the activation of Wnt/β-catenin pathway.

    27. Co-expression analysis reveals key gene modules and pathway of human coronary heart disease

      Yu Tang, Zun-Ping Ke, Yi-Gen Peng and Ping-Tai Cai

      Version of Record online: 20 NOV 2017 | DOI: 10.1002/jcb.26372

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      Our results showed that modules 9 and 10 played critical roles in the occurrence of coronary disease. Pathways of hsa00190 (oxidative phosphorylation) and (hsa01130: biosynthesis of antibiotics)were thought to be closely related to the occurrence and development of coronary heart disease. Our result demonstrated that modules 9 and 10 were the most critical modules in the occurrence of coronary heart disease. Pathways as hsa00190 (oxidative phosphorylation) and (hsa01130: biosynthesis of antibiotics) had the potential to serve as the prognostic and predictive marker of coronary heart disease.

    28. New insights into the molecular characteristics behind the function of Renilla luciferase

      Zahra Fanaei-Kahrani, Mohamad Reza Ganjalikhany, Seyed Mohammad Mahdi Rasa and Rahman Emamzadeh

      Version of Record online: 16 NOV 2017 | DOI: 10.1002/jcb.26339

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      According to computational results, it was suggested that Renilla luciferase has an induced-fit enzymatic reaction. A lid-like structure and its functional motions during substrate binding was proposed. It was computationally proved that binding energy in native RLuc is much higher than Super RLuc8. Also, we proposed the contribution of each active site residue in binding energy.

    29. You have full text access to this OnlineOpen article
      1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol (PLAG) reduces hepatic injury in concanavalin A-treated mice

      Young E. Ko, Sun Y. Yoon, Sun Y. Ly, Joo H. Kim, Ki Y. Sohn and Jae W. Kim

      Version of Record online: 16 NOV 2017 | DOI: 10.1002/jcb.26299

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      Liver damage was evaluated by histological observation in PLAG-treated mouse livers.The ability of PLAG to suppress the phosphorylation of the PKC-ζ/STAT6 pathway resulted in inhibition of hepatic cytokine expression in the plasma, the expression of adhesion molecules, and neutrophil migration.

    30. MicroRNA-410 is involved in mitophagy after cardiac ischemia/reperfusion injury by targeting high-mobility group box 1 protein

      Fan Yang, Tong Li, Zhihuan Dong and Rui Mi

      Version of Record online: 16 NOV 2017 | DOI: 10.1002/jcb.26405

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      Our results suggest that miR-410 may inhibit mitophagy after cardiac I/R injury by modulating HSPB1 activity via directly targeting HMGB1.

    31. Human biliverdin reductase regulates the molecular mechanism underlying cancer development

      Min Zhang, Wei Xin, Zhi Yi, Yue Li, Ying Liu, Hongyue Zhang, He Chen, Xinxin Chen, Shujie Tan and Daling Zhu

      Version of Record online: 14 NOV 2017 | DOI: 10.1002/jcb.26285

      The results of this study showed that hBVR expression was up-regulated in breast, lung, and liver cancers, but not ovarian cancer. Moreover, hBVR inhibition by small interfering RNA attenuated cell survival and increased caspase-3 protein expression, which was mediated by the ERK1/2 signaling pathway in relative cancer cells. In addition, the mitochondrial membrane potential and mitochondrial membrane proteins Bcl-2 and BAX were found to be necessary for activation of the mitochondria dependent apoptosis pathway and induction of the anti-apoptotic effect of hBVR in cancer cells.

    32. Impairment of primary cilia contributes to visceral adiposity of high fat diet-fed mice

      Ni Qiu, Wei-Jin Fang, Hong-Sheng Li, Zhi-Min He, Zhou-Sheng Xiao and Yan Xiong

      Version of Record online: 14 NOV 2017 | DOI: 10.1002/jcb.26253

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      The proadipogeic effects of abnormal primary cilia formation were evaluated in VAT-SVFs derived from HFD-induced obese mice. HFD-induced obesity resulted in severe dysfunction of primary cilia, leading to enhanced adipogenic potentials due to promoting cell cycle re-entry in VAT preadipocytes.

  2. Letters to the Editor

    1. Dissection of Regulatory Elements During Direct Conversion of Somatic Cells Into Neurons

      Hossein Fallahi, Tahereh Soleimani and Nafiseh Falsafi

      Version of Record online: 14 NOV 2017 | DOI: 10.1002/jcb.26112

  3. RESEARCH ARTICLES

    1. Interleukin-1 receptor-associated kinase 1 correlates with metastasis and invasion in endometrial carcinoma

      Yilin Wang, Yanyan Wang, Xianzhi Duan, Yinuo Wang and Zhenyu Zhang

      Version of Record online: 14 NOV 2017 | DOI: 10.1002/jcb.26416

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      IRAK1 was found elevated in endometrial carcinoma compared with normal tissues, and correlated with higher tumor stage, lymph node metastasis, myometrial invasion, and lower survival rate. Downregulation of IRAK1 significantly inhibit tumor growth, metastasis, and invasion. GSEA on TCGA dataset showed that KEGG mitotic cell cycle and cell division pathways were correlative with the IRAK1 expression, which was further confirmed in EC cells by Western blot.

  4. BENCHMARKS

    1. Cytokine profiling of MCF-7 cell line 2D, progressive 3D, and 3D revert cultures

      Aishwarya Subramaniyan and Ravi Maddaly

      Version of Record online: 10 NOV 2017 | DOI: 10.1002/jcb.26349

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      3D cultures of cancer cell lines have gained importance for several applications. Obtaining 3D Reverts from the 3D aggregates is an extension of culture methods that can enhance the utility of cancer cell lines. The cytokine expression differences of MCF-7 cell line when cultured as 2D monolayers, 3D aggregates, and as 3D reverts are presented here.

  5. RESEARCH ARTICLES

    1. Genistein represses PEPCK-C expression in an insulin-independent manner in HepG2 cells and in alloxan-induced diabetic mice

      Barilin Dkhar, Kitboklang Khongsti, Daiahun Thabah, Donkupar Syiem, Kapaettu Satyamoorthy and Bidyadhar Das

      Version of Record online: 10 NOV 2017 | DOI: 10.1002/jcb.26356

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      Genistein regulates PEPCK-C through the modulation of AMPK-CRTC2 and MEK/ERK signaling pathways, which may allow a novel approach to modulate dysfunction in hepatic gluconeogenesis in type 2 diabetes mellitus.

    2. Identification of novel inhibitors against UDP-galactopyranose mutase to combat leishmaniasis

      Mohammad Kashif, Shams Tabrez, Atahar Husein, Mohd Arish, Ponnusamy Kalaiarasan, Partha P. Manna, Naidu Subbarao, Yusuf Akhter and Abdur Rub

      Version of Record online: 10 NOV 2017 | DOI: 10.1002/jcb.26433

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      In this study, we have selected UDP-galactopyranose mutase (UGM), an enzyme involved in the synthesis of β-Galactofuranose (β-Galf), a major component of leishmanial membrane. Due to its absence in human, this enzyme has been targeted to search new antileishmanial drugs. Three dimensional model of Leishmania major UGM (LmUGM) has been generated using Trypanosoma cruzi UGM (TcUGM) as template and screened for reported active compounds from PubChem database to target it. Inhibitor having pubchem id: 6064500 has been proven as the best screened ligand of Lm-UGM which was further validated by in vitro antileishmanial activity assay. It has exhibited comparatively and significantly lesser toxicity than miltefosine hydrate (known antileishmanial drug) in mammalian cells when treated with the same concentrations. This is the first study targeting Lm-UGM for the screening of the proposed potential inhibitors to treat leishmaniasis in the best of our knowledge.

    3. LincRNA DYN-LRB2-2 upregulates cholesterol efflux by decreasing TLR2 expression in macrophages

      Yongqiang Li, Shuxin Shen, Shoukun Ding and Lixia Wang

      Version of Record online: 10 NOV 2017 | DOI: 10.1002/jcb.26352

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      Our study indicated that lincRNA DYN-LRB2-2 could upregulates cholesterol efflux, at least partially by decreasing TLR2 expression in macrophages. This study is helpful for understanding the molecular mechanism of the pregress and development of atherosclerosis.

    4. In vivo and in vitro effects of microRNA-124 on human gastric cancer by targeting JAG1 through the Notch signaling pathway

      Hai-Juan Xiao, Qing Ji, Lin Yang, Ren-Ting Li, Cheng Zhang and Jun-Ming Hou

      Version of Record online: 8 NOV 2017 | DOI: 10.1002/jcb.26413

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      The demonstration that miR-124 inhibits GC cell growth supports the concept that miR-124 functions as a tumor suppressor by a mechanism that involves translational repression of the JAG1 and the inhibition of Notch signaling pathway.

    5. Stem cells and differentiated cells differ in their sensitivity to urine in vitro

      Marta Pokrywczyńska, Tomasz Kloskowski, Daria Balcerczyk, Monika Buhl, Arkadiusz Jundziłł, Maciej Nowacki, Kaja Męcińska-Jundziłł and Tomasz Drewa

      Version of Record online: 1 NOV 2017 | DOI: 10.1002/jcb.26393

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      In this study, we compared the sensitivity of three different cell lines to urine environment in vitro. Differentiated cells isolated from bladder biopsy seems to be more suitable for regeneration of urinary tract, however, these cells have limited application especially in the case of urinary bladder cancer. ADSCs were the most sensitive on urine among all tested cell lines, in order to reduce sensitivity of MSCs on urine, initial predifferentiation could be performed before implantation which could increase their viability.

    6. Nrf2 transfection enhances the efficacy of human amniotic mesenchymal stem cells to repair lung injury induced by lipopolysaccharide

      Shouqin Zhang, Wei Jiang, Lijie Ma, Yuhao Liu, Xiangyu Zhang and Sheng Wang

      Version of Record online: 1 NOV 2017 | DOI: 10.1002/jcb.26322

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      Compared with control hAMSCs, treatment with Nrf2-overexpressing hAMSCs led to further reduced lung injury, lung fibrosis, and inflammation in LPS-challenged mice. Nrf2-overexpressing hAMSCs also exhibited increased cell retention in the lung and more efficient differentiation into AT II cells than control.

    7. Role of pncA gene mutations W68R and W68G in pyrazinamide resistance

      Mansi Aggarwal, Aditi Singh, Sonam Grover, Bharati Pandey, Anchala Kumari and Abhinav Grover

      Version of Record online: 1 NOV 2017 | DOI: 10.1002/jcb.26420

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      In this study, we conducted several analyses on native and mutant structures (W68R, W68G) of Pyrazinamidase (PZase) before and after docking with the PZA drug to explore the mechanism behind PZA resistance. Molecular dynamics simulation analyses showed that mutation in the 68th residue of protein results in a structural change at its active site which further affects the biological function of protein, that is, to act as an enzyme and convert PZA into pyrazinoic acid (POA). The mutations in the protein thereby led to PZA resistance in the bacterium due to the inefficient binding.

    8. Effects of placebo-controlled insulin-sensitizing drugs on hormonal parameters in polycystic ovary syndrome patients: A network meta-analysis

      Rong Huang, Peng-Fei Zhao, Jian-Hua Xu, Dong-Dong Liu, Fu-Dong Luo and Yong-Hui Dai

      Version of Record online: 30 OCT 2017 | DOI: 10.1002/jcb.26410

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      We conducted a network meta-analysis to compare effects of different placebo-controlled insulin-sensitizing drugs, including metformin, pioglitazone, rosiglitazone, and troglitazone on hormonal parameters in polycystic ovary syndrome (PCOS) patients. Our results indicated that rosiglitazone had the best effect on PCOS patients in terms of DHEAS, TT, FSH, and LH, metformin had the best effect on PCOS patients for E2, FT, and androstenedione.

    9. The roles of interleukin-1 and RhoA signaling pathway in rat epilepsy model treated with low-frequency electrical stimulation

      Ai-Hua Liu, Ya-Ting Wu, Li-Ping Li and Yu-Ping Wang

      Version of Record online: 30 OCT 2017 | DOI: 10.1002/jcb.26415

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      IL-1 might inhibit the efficacy of low-frequency ES for epilepsy via the RhoA/ROCK signaling pathway, which may provide a theoretical basis for clinical treatment of epilepsy.

    10. The diversity in the expression profile of caveolin II transcripts, considering its new transcript in breast cancer

      Mehdi Ariana, Nasibeh Arabi, Majid Pornour, Hajar Vaseghi, Shahla Mohammad Ganji, Mohammad Reza Alivand, Maryam Salari and Mohammad Esmaeil Akbari

      Version of Record online: 30 OCT 2017 | DOI: 10.1002/jcb.26378

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      Differential expression of cavolin's variant in breast cancer.

    11. FGFR2 regulation by picrasidine Q inhibits the cell growth and induces apoptosis in esophageal squamous cell carcinoma

      Yuanyuan Shi, Xuejiao Liu, Mangaladoss Fredimoses, Mengqiu Song, Hanyong Chen, Kangdong Liu, Mee-Hyun Lee and Zigang Dong

      Version of Record online: 27 OCT 2017 | DOI: 10.1002/jcb.26385

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      FGFR2 is highly expressed in ESCC patients. FGFR2 is a direct target of Picrasidine Q. Picrasidine Q inhibits cell growth of ESCC cells. Picrasidine Q suppresses down-stream signaling pathways of FGFR2.

    12. Intranasal insulin treatment restores cognitive deficits and insulin signaling impairment induced by repeated methamphetamine exposure

      Elmira Beirami, Shahrbanoo Oryan, Seyedeh Masoumeh Seyedhosseini Tamijani, Abolhassan Ahmadiani and Leila Dargahi

      Version of Record online: 27 OCT 2017 | DOI: 10.1002/jcb.26398

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      Repeated methamphetamine exposure induced cognitive deficits concomitant with insulin signaling impairment and mitochondrial dysfunctions. Intranasal insulin treatment alleviated MA-induced cognitive deficits, insulin signaling impairment, and mitochondrial dysfunctions.

    13. The inhibitory effect of 5,7-DMF on pancreatic sphere-forming cell function mediated by FoxM1 gene expression

      Deyu Zeng, Jian Ma, Rongrong Li, Jianfeng Yang and Xianli Yin

      Version of Record online: 27 OCT 2017 | DOI: 10.1002/jcb.26346

      The inhibition of pancreatic cancer stem cells was mediated by the expression of FoxM1 gene, and also the expression of SOX2 gene was regulated by FoxM1 in pancreatic cancer stem cells and involved in the inhibitory role of 5,7-DMF. These results provided important basis for the application of 5,7-DMF as a novel drug candidate for the pancreatic cancer treatment.

    14. The association of the placental MTHFR 3′-UTR polymorphisms, promoter methylation, and MTHFR expression with preeclampsia

      Abbas Mohammadpour-Gharehbagh, Batool Teimoori, Mehrnaz Narooei-nejad, Mehrnaz Mehrabani, Ramin Saravani and Saeedeh Salimi

      Version of Record online: 27 OCT 2017 | DOI: 10.1002/jcb.26290

      The MTHFR mRNA expression was lower in PE women. The MTHFR rs1537514C>G polymorphism was associated with lower PE risk and MTHFR mRNA expression. Lower expression of MTHFR mRNA was observed in the women with the hypermethylated promoter.

    15. Sleep deprivation induces pathological changes in rat masticatory muscles: Role of Toll like signaling pathway and atrophy

      Veronica Q. Yujra, Hanna K. M. Antunes, Marcos Mônico-Neto, Luciana P. Pisani, Aline B. Santamarina, Hananiah T. Quintana, Flavia de Oliveira, Celina T. F. Oshima and Daniel A. Ribeiro

      Version of Record online: 27 OCT 2017 | DOI: 10.1002/jcb.26389

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      Sleep deprivation in rat masticatory muscles being Toll like signaling pathway and muscle atrophy involved to this process at molecular level.

    16. SHH gene silencing suppresses epithelial-mesenchymal transition, proliferation, invasion, and migration of cervical cancer cells by repressing the hedgehog signaling pathway

      Feng Zhang, Chen-Chen Ren, Ling Liu, Yan-Nan Chen, Li Yang, Xiao-An Zhang, Xiao-Ming Wang and Feng-Jing Yu

      Version of Record online: 27 OCT 2017 | DOI: 10.1002/jcb.26414

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      SHH gene silencing suppresses EMT, proliferation, invasion, and migration of CC cells through the repression of the Hedgehog signaling pathway.

    17. Phospholipid secretions of organ cultured ciliary body

      Michael J. Margolis, Mitchell Martinez, Jeffrey Valencia, Richard K. Lee and Sanjoy K. Bhattacharya

      Version of Record online: 27 OCT 2017 | DOI: 10.1002/jcb.26419

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      Anterior chamber structures such as trabecular meshwork (TM) and fluid: aqueous humor (AH) helps in maintenance of intraocular pressure homeostasis. Our results demonstrate that AH phospholipids, unlike its other constituents, is not entirely produced by the ciliary body (CB). We further demonstrate that the drugs to treat glaucoma such as timolol, alters the release of phospholipids by the CB. We also present evidence that the released CB lipids play a critical role on health and survival of cultured primary TM cells and TM tissue.

    18. Effects of cyclin D1 gene silencing on cell proliferation, cell cycle, and apoptosis of hepatocellular carcinoma cells

      Jin Chen, Xue Li, Qi Cheng, Deng Ning, Jie Ma, Zhi-Ping Zhang, Xiao-Ping Chen and Li Jiang

      Version of Record online: 27 OCT 2017 | DOI: 10.1002/jcb.26400

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      Cyclin D1 gene silencing suppresses cell proliferation and inhibits cell apoptosis, which may be a new target approach in the treatment and management for HCC.

    19. Procaine is a specific DNA methylation inhibitor with anti-tumor effect for human gastric cancer

      Yong-Chao Li, Yun Wang, Dan-Dan Li, Ying Zhang, Tian-Cheng Zhao and Chang-Feng Li

      Version of Record online: 27 OCT 2017 | DOI: 10.1002/jcb.26407

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      In this study, we investigated the influence of procaine on DNA methylation as well as on cell proliferation and apoptosis of gastric cancer cells. We found that procaine repressed the activity but not the expression of DNMT1 and DNMT3A. Further evidence showed that procaine increased the expression of CDKN2A and RARβ through activating the promoter activity of those genes, which is determined by the DNA methylation level. Functionally, procaine inhibited the proliferation and induced the apoptosis of gastric cancer cells. Our results describe a critical role of procaine in the expression of DNMT1 and some critical target genes and suggest the procaine treatment might be a new therapeutic strategy for gastric cancer.

    20. Cloning, expression pattern analysis, and subcellular localization of Capra hircus SCD1 gene with production of transgenic mice

      Qisheng Zuo, Kai Jin, Jiuzhou Song, Yani Zhang and Bichun Li

      Version of Record online: 24 OCT 2017 | DOI: 10.1002/jcb.26386

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      Its successful to produce SCD1 transgenic mice with successful expression of sperm and tail tissue in the F1 mice generation.

    21. A TP73-AS1/miR-200a/ZEB1 regulating loop promotes breast cancer cell invasion and migration

      Qiongyan Zou, Enxiang Zhou, Feng Xu, Danhua Zhang, Wenjun Yi and Jia Yao

      Version of Record online: 24 OCT 2017 | DOI: 10.1002/jcb.26380

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      We demonstrated a TP73-AS1/miR-200a/ZEB1 regulating loop in breast cancer cells, which promote cancer cell invasion and migration through regulating metastasis-related factors. Suppressing TP73-AS1 expression to rescue miR-200a expression, thus to inhibit ZEB1 and Twist expression and up-regulate E-cadherin might improve breast cancer cell invasion and migration.

    22. Expression of nestin in embryonic tissues and its effects on clinicopathological characteristics of patients with placenta previa

      Yan-Yan Qiao and Ping Chu

      Version of Record online: 24 OCT 2017 | DOI: 10.1002/jcb.26368

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      Findings of this study suggested that, nestin, as a specific marker of neural precursor cells, was expressed in various tissues of the embryo in patients with placenta previa and nestin expression was lower with increased maturation of the embryo.

    23. miR-377-3p drives malignancy characteristics via upregulating GSK-3β expression and activating NF-κB pathway in hCRC cells

      Wei-Ying Liu, Zhen Yang, Qi Sun, Xi Yang, Yang Hu, Hong Xie, Hui-Jie Gao, Li-Ming Guo, Jian-Ying Yi, Min Liu and Hua Tang

      Version of Record online: 23 OCT 2017 | DOI: 10.1002/jcb.26374

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      miR-377-3p directly upregulates GSK-3β expression, which facilitates activation of the NF-κB to contribute to the NF-κB dependent malignancy characteristics in CRC cells. These findings reveal an oncogenic function of miR-377-3p in CRC cells, which may provide new insight into the molecular mechanism between miRNAs and NF-κB pathway.

    24. Interleukin-6 promotes the migration and cellular senescence and inhibits apoptosis of human intrahepatic biliary epithelial cells

      Ran Li, Juan Dong, Xiu-Qin Bu, Yong Huang, Jing-Yu Yang, Xuan Dong and Jie Liu

      Version of Record online: 20 OCT 2017 | DOI: 10.1002/jcb.26375

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      IL-6 may stimulate EMT, enhance the migration and proliferation, and inhibit apoptosis of HIBECs, thus delaying cellular senescence.

    25. Silencing of SOCS-1 and SOCS-3 suppresses renal interstitial fibrosis by alleviating renal tubular damage in a rat model of hydronephrosis

      Gui-Hong Zheng, Yong-Jian Wang, Xin Wen, Xin-Rui Han, Min Shen, Shan Wang, Juan Zhuang, Zi-Feng Zhang, Liang Wang, Bin Hu, Chun-Hui Sun, Xiao-Yun Mao, Lan-Ni Yi, Dong-Mei Wu, Jun Lu and Yuan-Lin Zheng

      Version of Record online: 20 OCT 2017 | DOI: 10.1002/jcb.26382

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      Our study demonstrated that silencing of SOCS-1/3 may suppress RIF by alleviating the renal tubular damage in rat models affected by hydronephrosis.

  6. PROSPECTS

    1. A CRISPR reimagining: New twists and turns of CRISPR beyond the genome-engineering revolution

      Robert J. Plummer, Yi Guo and Ying Peng

      Version of Record online: 20 OCT 2017 | DOI: 10.1002/jcb.26406

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      Despite its explosive applications in genome engineering, CRISPR was developed into a versatile tool beyond its nuclease function. In this article, we summarize several exciting applications including manipulating DNA sequences, visualizing chromosomal loci in living cells, and modulating transcription and chromatin structures. These novel applications will elevate CRISPR into another level of sophistication and diversity, leading to many exciting cell biological discoveries.

  7. RESEARCH ARTICLES

    1. Kaempferol increases apoptosis in human acute promyelocytic leukemia cells and inhibits multidrug resistance genes

      Maliheh Moradzadeh, Alijan Tabarraei, Hamid Reza Sadeghnia, Ahmad Ghorbani, Ashraf Mohamadkhani, Saiedeh Erfanian and Amirhossein Sahebkar

      Version of Record online: 20 OCT 2017 | DOI: 10.1002/jcb.26391

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      This study investigates the potential therpaeutic effects of kaempferol in acute promyelocytic leukemia.

    2. MicroRNA-142-5p induces cancer stem cell-like properties of cutaneous squamous cell carcinoma via inhibiting PTEN

      Xinping Bai, Yu Zhou, Ping Chen, Ming Yang and Jiang Xu

      Version of Record online: 20 OCT 2017 | DOI: 10.1002/jcb.26379

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      In our study, it was identified that hsa-miR-142-5p as a novel oncogene in cSCC. We indicated that hsa-miR-142-5p can trigger cancer stem cell-like properties through inhibition of PTEN in cSCC in which Wnt signaling is involved. Our findings might suggest hsa-miR-142-5p can act as a novel therapeutic target for cSCC.

    3. Ouabain attenuates the oxidative stress induced by lipopolysaccharides in the cerebellum of rats

      Israel José Pereira Garcia, Paula Fernanda Kinoshita, Italo de Oliveira Braga, Gabriela Machado Parreira, Julio Alberto Mignaco, Cristoforo Scavone, Leandro Augusto Barbosa and Hérica de Lima Santos

      Version of Record online: 18 OCT 2017 | DOI: 10.1002/jcb.26377

      Our data suggest a neuroprotective effect of OUA against LPS-induced oxidative stress in rat cerebellum, by promoting membrane lipid remodeling, and by increasing the expression of the glutamate transporter EAAT4. It is important to note that OUA did not alter the activity of Na,K-ATPase, suggesting that in this case the enzyme might be acting solely as a cell receptor, may lead to a possible activation of signaling cascades, that could alleviate some cell effects of LPS.

    4. A novel splicing isoform of protein arginine methyltransferase 1 (PRMT1) that lacks the dimerization arm and correlates with cellular malignancy

      Odysseas Patounas, Ioanna Papacharalampous, Carmen Eckerich, Georgios S. Markopoulos, Evangelos Kolettas and Frank O. Fackelmayer

      Version of Record online: 18 OCT 2017 | DOI: 10.1002/jcb.26373

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      This article characterizes a novel splicing isoform of the predominant member of the human PRMT (protein arginine methyltransferase) family that correlates with cellular malignancy. The isoform lacks the dimerization arm of the enzyme, and is catalytically inactive, but able to bind substrates, and blocks cells in the G1 phase of the cell cycle.

    5. Characterization of purine-rich element binding protein B as a novel biomarker in acute myelogenous leukemia prognostication

      Robert J. Kelm Jr, Gurpreet S. Lamba, Jamie E. Levis and Chris E. Holmes

      Version of Record online: 18 OCT 2017 | DOI: 10.1002/jcb.26369

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      Purine-rich element binding protein B (PURB) is a sequence-specific, single-stranded DNA-binding factor implicated in the regulation of blood cell differentiation. Expression of the PURB gene is most prominent in hematopoietic stem/progenitor cells and in transformed myeloid cells. Enhanced single-stranded DNA-binding activity of the PURB protein is a phenotypic feature of CD33 positive leukocytes isolated from specific subsets of patients diagnosed with acute myelogenous leukemia.

  8. RESEARCH ARTICLES (FASTTRACK)

    1. Oroxylin A prevents angiogenesis of LSECs in liver fibrosis via inhibition of YAP/HIF-1α signaling

      Chenxi Zhang, Mianli Bian, Xingran Chen, Huanhuan Jin, Shifeng Zhao, Xiang Yang, Jiangjuan Shao, Anping Chen, Qinglong Guo, Feng Zhang and Shizhong Zheng

      Version of Record online: 18 OCT 2017 | DOI: 10.1002/jcb.26388

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      Oroxylin A inhibited hypoxia-induced nuclear translocation of YAP, which may influence the accumulation of HIF-1α and subsequently decrease transcription of downstream target gene including VEGF-A and Ang-2, thereby exerting an anti-angiogenic activity.

  9. RESEARCH ARTICLES

    1. Quercetin reduces neural tissue damage and promotes astrocyte activation after spinal cord injury in rats

      Yeyang Wang, Wenjun Li, Mingsen Wang, Chuangxin Lin, Guitao Li, Xiaozhong Zhou, Junnan Luo and Dadi Jin

      Version of Record online: 18 OCT 2017 | DOI: 10.1002/jcb.26392

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      Taken together, Que promoted locomotor functional recovery and astrocyte activation and axonal regeneration after acute SCI, possibly through the BDNF and JAK2/STAT3 signaling pathways.

    2. ZIC2 promotes viability and invasion of human osteosarcoma cells by suppressing SHIP2 expression and activating PI3K/AKT pathways

      Shuaihao Huang and Anmin Jin

      Version of Record online: 18 OCT 2017 | DOI: 10.1002/jcb.26387

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      ZIC2 is highly expressed in osteosarcoma cells and tissues. ZIC2 overexpression promotes viability, invasion, and metastasis of osteosarcoma cells. ZIC2 suppression inhibits viability, invasion, and metastasis of osteosarcoma cells. SHIP2 expression is negatively regulated by ZIC2. Activation of the PI3K/AKT signal pathway is essential for the effects of ZIC2 in osteosarcoma cells.

    3. Curcumin alleviates IL-17A-mediated p53-PAI-1 expression in bleomycin-induced alveolar basal epithelial cells

      Mahesh M. Gouda, Ashwini Prabhu and Yashodhar P. Bhandary

      Version of Record online: 18 OCT 2017 | DOI: 10.1002/jcb.26384

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      This investigation was designed to understand the changes associated with 1L-17A mediated up-regulation of p53 and PAI-1 expression and the role of curcumin in attenuating this process. Curcumin has the ability to regulate inflammatory cytokines during BLM induced injury and their effect on p53-PAI-1 expression. It can be used as a potential anti-inflammatory and anti-fibrinolytic component for intervening the epithelial cell damage.

    4. Autophagy-dependent crosstalk between GILT and PAX-3 influences radiation sensitivity of human melanoma cells

      Jessica D. Hathaway-Schrader, Bently P. Doonan, Azim Hossain, Faisal F.Y. Radwan, Lixia Zhang and Azizul Haque

      Version of Record online: 18 OCT 2017 | DOI: 10.1002/jcb.26383

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      Melanoma represents an ever increasing problem in the western world as incidence rates continue to climb. Though manageable during early stages, late stage metastatic disease is highly resistant to current intervention. Here we show that the expression of a thiol reductase (GILT) inhibits a potential tumorigenic molecule, paired box-3 (PAX-3), in late stage human metastatic melanoma, and increases the susceptibility of melanoma cells to low-dose radiation therapy via upregulation of p53.

    5. Effects of Glut1 gene silencing on proliferation, differentiation, and apoptosis of colorectal cancer cells by targeting the TGF-β/PI3K-AKT-mTOR signaling pathway

      Xue-Liang Wu, Li-Kun Wang, Dong-Dong Yang, Ming Qu, Yong-Jiang Yang, Fei Guo, Lei Han and Jun Xue

      Version of Record online: 18 OCT 2017 | DOI: 10.1002/jcb.26399

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      These results suggested that Glut1 gene silencing inhibits proliferation and promotes apoptosis of CRC cells by inactivating TGF-β/PI3K-AKT-mTOR signaling pathway.

    6. Leptin induces matrix metalloproteinase 7 expression to promote ovarian cancer cell invasion by activating ERK and JNK pathways

      Ahmad Ghasemi, Seyed Isaac Hashemy, Mahmoud Aghaei and Mojtaba Panjehpour

      Version of Record online: 18 OCT 2017 | DOI: 10.1002/jcb.26396

      Leptin induces ovarian cancer cell invasion via up-regulating Matrix Metalloproteinase 7 (MMP7). OB-Rb is involved in leptin-induced MMP7 expression. ERK and JNK inhibitors attenuate leptin-induced MM expression.

    7. Effects of long-term low oxygen tension in human chondrosarcoma cells

      Juha Piltti, Joakim Bygdell, Chengjuan Qu and Mikko J. Lammi

      Version of Record online: 18 OCT 2017 | DOI: 10.1002/jcb.26394

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      Long-term low oxygen tension increased a number of gene expressions, while elevated ACAN and COL2A1 expressions did not reveal changes at secretion levels during the 4-week exposure period. Still, the low oxygen tension-induced trend of the expression of ACAN and COL2A1 was consistent and, therefore, we suggest that 5% low oxygen condition could act as a supportive factor for the known chondrogenicity and/or extracellular matrix production enhancing factors, such as bone morphogenetic protein 4 or Rho-kinase inhibitor Y-27632.

    8. Stathmin/Op18 depletion induces genomic instability and leads to premature senescence in human normal fibroblasts

      Deepmala Shrestha, Namil Kim and Kiwon Song

      Version of Record online: 18 OCT 2017 | DOI: 10.1002/jcb.26401

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      We found that stathmin depletion leads to multipolar spindle and genomic instability in IMR-90 normal human fibroblasts. Genomic instability induced by stathmin depletion induced premature senescence without any indication of cell death in normal IMR-90 cells, while it triggered apoptosis even in p53-proficient cancer cells.

    9. Regulation of fibroblast growth factor 8 (FGF8) in chicken embryonic stem cells differentiation into spermatogonial stem cells

      Man Wang, Chen Zhang, Chuanli Huang, Shaoze Cheng, Nana He, Yilin Wang, Mahmoud F. Ahmed, Ruifeng Zhao, Jing Jin, Qisheng Zuo, Yani Zhang and Bichun Li

      Version of Record online: 18 OCT 2017 | DOI: 10.1002/jcb.26402

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      Here, we explored the effect of FGF8 on chicken SSCs formation by knockdown or overexpression of FGF8 in chicken embryonic stem cells (ESCs) both in vitro and in vivo. Our study demonstrated that FGF8 can regulate germ cell fate by modulating the dynamic equilibrium between differentiation and self-renewal, which provides a new idea for the study of germ cell regulatory network.

    10. Exploring the interaction between Mycobacterium tuberculosis enolase and human plasminogen using computational methods and experimental techniques

      Amit Rahi, Alisha Dhiman, Damini Singh, Andrew M. Lynn, Mohd Rehan and Rakesh Bhatnagar

      Version of Record online: 18 OCT 2017 | DOI: 10.1002/jcb.26403

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      Current work aims at exploring the role of enolase present on Mtb's cell surface in interaction with the host's plasminogen. Further, using various suitable computational methods the key amino acid residues involved in this event have been predicted and then mutated singly as well as in combinantions to confirm their engagement in plasminogen binding. Plasminogen being a major matrix protein of the host tissues is exploited by several pathogens as a proteolytic tool after being activated to the Plasmin which is a broad spectrum protease.

    11. Anti-apoptosis endothelial cell-secreted microRNA-195-5p promotes pulmonary arterial smooth muscle cell proliferation and migration in pulmonary arterial hypertension

      Zhen Zeng, Jun Yao, Yinchuan Li, Ying Xue, Yinghua Zou, Zhuoling Shu and Zhihua Jiao

      Version of Record online: 17 OCT 2017 | DOI: 10.1002/jcb.26376

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      There are crosstalk between apoptosis-resistant endothelial cells (AR-PVECs) and pulmonary arterial smooth muscle cells (PASMCs) in the progress of irreversible remodeling of the pulmonary vasculature. The high expressed of miRNA-195-5p by apoptosis-resistant endothelial cells can play a role as an interacting paracrine factor between AR-PVECs and PASMC. We confirmed the HIF-1a/miRNA-195-5p/Smad7 pathway is the mechanism in this cell to cell crosstalk.

    12. SIRT1 deacetylates KLF4 to activate Claudin-5 transcription in ovarian cancer cells

      Xinjian Zhang, Junliang Chen, Lina Sun and Yong Xu

      Version of Record online: 17 OCT 2017 | DOI: 10.1002/jcb.26404

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      SIRT1 activates Claudin 5 transcription in ovarian cancer cells. SIRT1 interacts with and deacetylates KLF4 in ovarian cancer cells. SIRT1 promotes nuclear accumulation and target promoter binding of KLF4.

    13. Down-regulation of the long noncoding RNA-HOX transcript antisense intergenic RNA inhibits the occurrence and progression of glioma

      Bin Yang, Zhen-Yu Wei, Bang-Qing Wang, Hua-Chao Yang, Jun-Yi Wang and Xing-Yao Bu

      Version of Record online: 13 OCT 2017 | DOI: 10.1002/jcb.26390

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      Our findings support that down-regulation of HOTAIR could inhibit cell proliferation, promote cell apoptosis as well as suppress cell invasion and migration in the progression of glioma.

    14. MAPK8 regulates chicken male germ cell differentiation through JNK signaling pathway

      Yingjie Wang, Yulin Bi, Qisheng Zuo, Wenhui Zhang, Dong Li, Na-na He, Shaoze Cheng, Ya-ni Zhang and Bichun Li

      Version of Record online: 11 OCT 2017 | DOI: 10.1002/jcb.26314

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      In this study, the specific role of JNK signaling pathway in the differentiation of ESCs to male germ cells was verified, and the signal pathway was activated/blocked in vitro and in vivo. The results showed that JNK signal pathway was positively regulated during the process of differentiation of male germ cells Role, on the basis of the channel and the upstream and downstream signal path between the regulatory role will be further verified.

    15. Actin Gamma 1, a new skin cancer pathogenic gene, identified by the biological feature-based classification

      Xinqian Dong, Yingsheng Han, Zhen Sun and Junlong Xu

      Version of Record online: 5 OCT 2017 | DOI: 10.1002/jcb.26301

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      Our investigation suggested that ACTG1 can regulate the cell proliferation and migration through ROCK signaling pathway.

    16. Knockdown of long non-coding RNA MEG3 protects H9c2 cells from hypoxia-induced injury by targeting microRNA-183

      Licheng Gong, Haiming Xu, Hong Chang, Yaliang Tong, Tao Zhang and Gongliang Guo

      Version of Record online: 5 OCT 2017 | DOI: 10.1002/jcb.26304

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      Our findings suggest that knockdown of MEG3 alleviates hypoxia-induced myocardial cell injury by targeting miR-183 and suppressing p27 expression through activation of PI3K/AKT/FOXO3a signaling pathways.

    17. Neferine modulates IGF-1R/Nrf2 signaling in doxorubicin treated H9c2 cardiomyoblasts

      Lohanathan Bharathi Priya, Rathinasamy Baskaran, Chih-Yang Huang and Viswanadha Vijaya Padma

      Version of Record online: 5 OCT 2017 | DOI: 10.1002/jcb.26305

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      Doxorubicin induced cardiotoxicity is a major problem during chemotherapy of cancers. Neferine treatment activated IGF-1R signaling, improved cellular antioxidant pool, increased the expression of down-stream targets of IGF-1R, PI3K/Akt/mTOR, inhibited mitochondrial superoxide generation and autophagy significantly with the induction of Nrf2 translocation and expressions of HO-1 and SOD1.

  10. PROSPECTS

    1. CDK9: A key player in cancer and other diseases

      Lia Carolina Franco, Fátima Morales, Silvia Boffo and Antonio Giordano

      Version of Record online: 5 OCT 2017 | DOI: 10.1002/jcb.26293

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      Cyclin-Dependent Kinase 9 (CDK9) is part of a functional diverse group of enzymes responsible for cell cycle control and progression. Recent studies have highlighted the importance of CDK9 in many relevant pathologic processes, like cancer, cardiovascular diseases, and viral replication. Herein we provide an overview of the different pathways in which CDK9 is directly and indirectly involved.

  11. RESEARCH ARTICLES

    1. Suppression of c-Myc enhances p21WAF1/CIP1-mediated G1 cell cycle arrest through the modulation of ERK phosphorylation by ascochlorin

      Yun-Jeong Jeong, Hyang-Sook Hoe, Hyun-Ji Cho, Kwan-Kyu Park, Dae-Dong Kim, Cheorl-Ho Kim, Junji Magae, Dong Wook Kang, Sang-Rae Lee and Young-Chae Chang

      Version of Record online: 4 OCT 2017 | DOI: 10.1002/jcb.26366

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      Our results showed that ascochlorin specifically inhibited the synthesis of the c-Myc protein regardless of proteasomal degradation. c-Myc stability was regulated by phosphorylation of ERK, p70S6 kinase (p70S6K), and 4EBP1, and inhibition of ERK phosphorylation enhanced c-Myc downregulation and p21WAF1/CIP1-dependent G1 cell cycle arrest.

    2. cAMP/PKA signaling pathway contributes to neuronal apoptosis via regulating IDE expression in a mixed model of type 2 diabetes and Alzheimer's disease

      Huajie Li, Song Yang, Jian Wu, Lei Ji, Linfeng Zhu, Liping Cao, Jinzhong Huang, Qingqing Jiang, Jiang Wei, Meng Liu, Keshi Mao, Ning Wei, Wei Xie and Zhilong Yang

      Version of Record online: 28 SEP 2017 | DOI: 10.1002/jcb.26321

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      Our study indicated that, in the T2D and AD mice, cAMP/PKA signaling pathway and IDE may participate in the contribute role of T2D in accelerating the pathological process of AD via causing the accumulation of Aβ and neuronal apoptosis.

    3. A prognostic 11 genes expression model for ovarian cancer

      Chuan-Di Men, Qiong-Na Liu and Qing Ren

      Version of Record online: 27 SEP 2017 | DOI: 10.1002/jcb.26358

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      The early stage symptoms of ovarian cancer are usually absent which make diagnosing in its early stages exceedingly difficult. Previous research has proven that ovarian cancer is a genetic disease, which depends on alteration of multi-cancer genes and anti-cancer genes, multi-stages and multi-pathways, involving a variety of oncogene activation and anti-oncogene inactivation. Then count the active number of influence factors based on the expression level of featured genes. When the number of influencing factors is ≥7, the prognosis is the largest (P-value = 0.000775); chosen this as the final Classification model. To summary, a prognostic 11 genes expression mode was preliminarily built to classify the ovarian cancer samples.

    4. SENP1/HIF-1α feedback loop modulates hypoxia-induced cell proliferation, invasion, and EMT in human osteosarcoma cells

      Xiaowei Wang, Xiaoju Liang, Huan Liang and Bing Wang

      Version of Record online: 27 SEP 2017 | DOI: 10.1002/jcb.26342

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      Positive feedback loop between HIF-1α and SENP1 in the regulating of the process of cell proliferation, invasion and EMT in OS cells under hypoxic conditions, suggesting that the SENP1/HIF-1α axis may serve as a new potential therapeutic agent for OS treatments.

    5. AAV delivery of GRP78/BiP promotes adaptation of human RPE cell to ER stress

      Shima Ghaderi, Shahin Ahmadian, Zahra-Soheila Soheili, Hamid Ahmadieh, Shahram Samiei, Samira Kheitan and Ehsan R. Pirmardan

      Version of Record online: 25 SEP 2017 | DOI: 10.1002/jcb.26296

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      To the best of our knowledge, this is the first report on AAV-mediated Grp78 overexpression in human primary RPE cultures, which validates GRP78/BiP as a key survival factor for ER- stressed hRPE cells and useful candidate for future investigations of RPE degenerative diseases.

    6. Wnt signaling pathway regulates differentiation of chicken embryonic stem cells into spermatogonial stem cells via Wnt5a

      Nana He, Yilin Wang, Chen Zhang, Man Wang, Yingjie Wang, Qisheng Zuo, Yani Zhang and Bichun Li

      Version of Record online: 25 SEP 2017 | DOI: 10.1002/jcb.26329

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      In this study, we investigated the mechanism of signaling pathway-mediated differentiation of embryonic stem cells (ESCs) into spermatogonial stem cells (SSCs) in chicken. The Wnt signaling pathway was identified based on previous RNA Sequencing results and was proven a crucial signaling pathway that participates in the differentiation of ESCs into SSCs.

    7. Differential expression profile analysis of lncRNA UCA1α regulated mRNAs in bladder cancer

      Yu Wang, Hong Zhang, Xu Li and Wei Chen

      Version of Record online: 25 SEP 2017 | DOI: 10.1002/jcb.26345

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      A total of 71 differential expression genes was identified, including 52 up-regulated genes and 19 down-regulated genes; differential expression genes exhibited several significantly biological process, cellular component, and molecular function; differential expression genes involved in several significant pathways.

    8. Breast cancer stem cells are involved in Trastuzumab resistance through the HER2 modulation in 3D culture

      Cristina E. Rodríguez, Damian E. Berardi, Marianela Abrigo, Laura B. Todaro, Elisa D. Bal de Kier Joffé and Gabriel L. Fiszman

      Version of Record online: 25 SEP 2017 | DOI: 10.1002/jcb.26298

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      Breast cancer human cells culture as spheroids develop autophagy and apoptosis, which promotes Trastuzumab resistance in HER2 overexpressing cells. Our aim was to study the association of the hostile environment developed in 3D with the breast cancer stem cells population and the HER2 modulation. Our findings show that 3D architecture is able to modulate breast cancer stem cell population and HER2 distribution, modifying the cell response to Trastuzumab.

  12. PROSPECTS

    1. A shared comparison of diabetes mellitus and neurodegenerative disorders

      Mahmoud Morsi, Ahmed Maher, Omnia Aboelmagd, Dina Johar and Larry Bernstein

      Version of Record online: 25 SEP 2017 | DOI: 10.1002/jcb.26261

  13. RESEARCH ARTICLES

    1. The N-terminal ubiquitin-associated domain of Cezanne is crucial for its function to suppress NF-κB pathway

      Yanxi Ji, Liu Cao, Lanyi Zeng, Zhen Zhang, Qiaoqiao Xiao, Penglin Guan, Shiyou Chen, Yu Chen, Min Wang and Deyin Guo

      Version of Record online: 21 SEP 2017 | DOI: 10.1002/jcb.26359

      Our results suggested that Cezanne can bind Lys63 polyubiquitin chains via Leu9 and Ser10 sites in the UBA domain, and Cezanne is recognized and recruited into activated TNFR complex by specifically binding to polyubiquitinated signaling proteins after TNF stimulation through its N-terminal polyubiquitin binding sites within UBA domain

    2. Autophagy induction plays a protective role against hypoxic stress in human dental pulp cells

      Sam-Young Park, Eun-Gene Sun, Yeonju Lee, Min-Seok Kim, Jae-Hyung Kim, Won-Jae Kim and Ji-Yeon Jung

      Version of Record online: 20 SEP 2017 | DOI: 10.1002/jcb.26360

      Thumbnail image of graphical abstract

      CoCl2-induced autophagy plays a protective role against cytotoxicity under hypoxic stress. In addition, Autophagy process is mediated by the suppression of the mTOR/p70S6K pathway regulating by HIF1 in CoCl2-treated HDPCs.

    3. Effects of gene knockdown of CNP on ventricular remodeling after myocardial ischemia-reperfusion injury through NPRB/Cgmp signaling pathway in rats

      Lian-He Wu, Qi Zhang, Shen Zhang, Lu-Yu Meng, Yan-Chi Wang and Cun-Jian Sheng

      Version of Record online: 18 SEP 2017 | DOI: 10.1002/jcb.26341

      Thumbnail image of graphical abstract

      In conclusion, the results in this study have demonstrated that gene knockdown of CNP blocks the NPRB/cGMP signaling pathway, which aggravates myocardial I/R injury and causes ventricular remodeling in rats. This study is helpful to investigate the roles of CNP and it would provide some important suggestions for the treatment and diagnosis of ischemic heart diseases in the future. However, this study did not include a large amount of samples, which would impact the results of the experiment to some extent. Therefore, more studies are needed to validate the conclusion in the present study in the following experimental researches.

    4. Curcumin converts Foxp3+ regulatory T cells to T helper 1 cells in patients with lung cancer

      Jian Y. Zou, Chun H. Su, Hong H. Luo, Yi Y. Lei, Bo Zeng, Hao S. Zhu and Zhen G. Chen

      Version of Record online: 18 SEP 2017 | DOI: 10.1002/jcb.26302

      The frequency of peripheral Treg in lung cancer patients was suppressed after treating with curcumin for 2 weeks. The peripheral Th1 cells were increased in lung cancer patients after the curcumin therapy. Curcumin converted the lung cancer-isolated Tregs to Th1 cells via repressing the gene transcription of forkhead protein-3 and increasing the expression of interferon-gamma.

    5. Naringenin inhibits osteoclastogenesis through modulation of helper T cells-secreted IL-4

      Wengang Wang, Mingjun Li, Ming Luo, Mingkui Shen, Chen Xu, Genzhong Xu, Yaokun Chen and Lei Xia

      Version of Record online: 18 SEP 2017 | DOI: 10.1002/jcb.26370

      Thumbnail image of graphical abstract

      Our results demonstrated the inhibitory effects of NAR on osteoclastogenesis and osteoclast bone resorption were dependent on T cells-secreted IL-4 in vitro. Our data strongly suggest that NAR can be developed as a therapeutic drug for osteoclast-mediated bone diseases.

    6. Necrotic cell death occur via JNK pathway with the activity of transcription factor c-Jun by 4-MC in INS-1 cell line

      Ayse Karatug Kacar and Sehnaz Bolkent

      Version of Record online: 18 SEP 2017 | DOI: 10.1002/jcb.26367

      Thumbnail image of graphical abstract

      The cells perform necrotic death by the following options: i) phosphorylated RAF1 activates the JNK pathway with the activity of transcription factor c-Jun, ii) Hsp 70 and Hsp 90 did not show a change inside the cell, rendering the JNK pathway active.

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