Journal of Cellular Physiology

Cover image for Vol. 232 Issue 1

Edited By: Gary S. Stein, Harvey M. Florman and Constance E. Brinckerhoff

Impact Factor: 4.155

ISI Journal Citation Reports © Ranking: 2015: 12/83 (Physiology); 63/187 (Cell Biology)

Online ISSN: 1097-4652

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Dissection of Individual Prostate Lobes in Mouse Models of Prostate Cancer to Obtain High Quality RNA

Areg Zingiryan, Nicholas H. Farina, Kristiaan H. Finstad, Janet L. Stein, Jane B. Lian and Gary S. Stein

A procedure with full color images to isolate individual murine prostate lobes, free of adjacent tissue, for use in downstream gene expression studies is presented. All eight murine prostate lobes can be harvested within an hour and processed for histologic analysis and molecular component isolation (RNA, DNA, protein, etc.). Only the lateral and ventral prostate lobes yield non-degraded RNA.

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