Journal of Cellular Physiology

Cover image for Vol. 231 Issue 8

Edited By: Gary S. Stein, Harvey M. Florman and Constance E. Brinckerhoff

Impact Factor: 3.839

ISI Journal Citation Reports © Ranking: 2014: 15/83 (Physiology); 71/184 (Cell Biology)

Online ISSN: 1097-4652

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CRISPR-Cas Assisted Multiplexing (CAM): Simple Same-Day Multi-Locus Engineering in Yeast

Jessica M. Walter, Sunil S. Chandran, and Andrew A. Horwitz

Installing complex biosynthetic pathways and combining point mutations in yeast are tedious and time-consuming using traditional methods. Here, we discuss the advantages and opportunities of CRISPR-Cas-assisted multiplexing (CAM), a same-day, cloning-free method for multi-locus engineering.

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