Journal of Cellular Physiology

Cover image for Vol. 230 Issue 10

Edited By: Gary S. Stein, Harvey M. Florman and Constance E. Brinckerhoff

Impact Factor: 3.839

ISI Journal Citation Reports © Ranking: 2014: 15/83 (Physiology); 71/184 (Cell Biology)

Online ISSN: 1097-4652

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Top Cited Articles 2014

Franchina, T., Amodeo, V., Bronte, G., Savio, G., Ricciardi, G. R.R., Picciotto, M., Russo, A., Giordano, A. and Adamo, V.
Circulating miR-22, miR-24 and miR-34a as Novel Predictive Biomarkers to Pemetrexed-Based Chemotherapy in Advanced Non-Small Cell Lung Cancer
Journal of Cellular Physiology 2014, vol. 229, p. 97

Basile, D. P. and Yoder, M. C.
Circulating and Tissue Resident Endothelial Progenitor Cells
Journal of Cellular Physiology 2014, vol. 229, p. 10

Anderson, M., Roshanravan, H., Khine, J. and Dryer, S. E.
Angiotensin II Activation of TRPC6 Channels in Rat Podocytes Requires Generation of Reactive Oxygen Species
Journal of Cellular Physiology 2014, vol. 229, p. 434

Zhang, Y.-Y., Yue, J., Che, H., Sun, H.-Y., Tse, H.-F. and Li, G.-R
BKCa and hEag1 Channels Regulate Cell Proliferation and Differentiation in Human Bone Marrow-Derived Mesenchymal Stem Cells
Journal of Cellular Physiology 2014, vol. 229, p. 202

Basile, D. P. and Yoder, M. C.
Circulating and Tissue Resident Endothelial Progenitor Cells
Journal of Cellular Physiology 2014, vol. 229, p. 10

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JCP Spotlight Article

Brg1 Controls the Expression of Pax7 to Promote Viability and Proliferation of Mouse Primary Myoblast

Teresita Padilla-Benavides, Brian T. Nasipak and Anthony N. Imbalzano

Brg1 (Brahma-related gene 1) is a catalytic component of the evolutionarily conserved mammalian SWI/SNF ATP-dependent chromatin remodeling enzymes that disrupt histone-DNA contacts on the nucleosome. While the requirement for the SWI/SNF enzymes in cell differentiation has been extensively studied, its role in precursor cell proliferation and survival is not as well defined. Muscle satellite cells constitute the stem cell pool that sustains and regenerates myofibers in adult skeletal muscle. Here, we show that deletion of Brg1 in primary mouse myoblasts derived from muscle satellite cells cultured ex vivo leads to a cell proliferation defect and apoptosis. We determined that Brg1 regulates cell proliferation and survival by controlling chromatin remodeling and activating transcription at the Pax7 promoter, which is expressed during somite development and is required for controlling viability of the satellite cell population. Reintroduction of catalytically active Brg1 or of Pax7 into Brg1-deficient satellite cells rescued the apoptotic phenotype and restored proliferation. These data demonstrate that Brg1 functions as a positive regulator for cellular proliferation and survival of primary myoblasts. Therefore, the regulation of gene expression through Brg1-mediated chromatin remodeling is critical not just for skeletal muscle differentiation but for maintaining the myoblast population as well.

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