Journal of Biochemical and Molecular Toxicology

Cover image for Vol. 30 Issue 1


Online ISSN: 1099-0461

Author Guidelines

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Author Guidelines

Online Submission Instructions

Journal of Biochemical and Molecular Toxicology is now receiving submitted manuscripts online at .

All manuscripts should now be submitted online. To submit a manuscript, launch your web browser and go to . Check for an existing user account by entering your email address in the space beneath the box that reads “Password Help.” If you are submitting for the first time, and you do not find an existing account, create a new account by clicking on the words “Create Account” in the top right corner of the screen and following the given instructions. If you experience difficulty during the submission process, please contact the editorial office.

General Instructions

Manuscripts should be written in English and should conform to the general style of the journal and specific instructions listed below. Although the Materials and Methods Section should be concise, sufficient experimental detail should be included to permit repetition. Do not include the same material in more than one section, e.g discussion in both Results and Discussion.

Form and Style

Type manuscripts with double spacing throughout and margins of at least 1 inch (25 mm). Indicate positions of tables and figures in the text using the left margin. Principal headings within the text should be typed in all capital letters and indented; subheadings should be typed, also indented, in upper and lower case letters; if a third level of heading is required, it should have a paragraph indent, underscoring, upper and lower case lettering, and, as with the primary and secondary headings, should be on a line separate from the text.

Full-Length Articles

These should include all of the following elements, each of which should start on a separate sheet of paper. They should not exceed the following size limits: Abstract, 150 words; Introduction, 550 words; Material and Methods - see General Instructions (above); Discussion - 550 words; Figures - 5; Tables - 5.

  • Title page–title of article, author(s), affiliation(s) with complete addresses
  • Word, figure and table counts: abstract; introduction; discussion, figures, tables
  • Running title of not more than 40 characters, including spaces, and suggestions for key word index entries
  • Mailing address of the person to whom proofs should be sent
  • Abstract
  • Introduction
  • Materials and Methods
  • Results
  • Discussion
  • Acknowledgments
  • Footnotes
  • References
  • Figure legends
  • Tables

Rapid Communications

These should not exceed four double-spaced pages, including tables and figures. The subdivision into Materials and Methods, Results, and Discussion can be omitted. A brief summary and a short title should be provided. These data of immediate importance to other researchers will be published rapidly.


Mini-reviews of timely importance will be published by invitation but submitted mini-reviews will also be considered. The manuscript should not exceed 15 typewritten pages and the subdivisions are at the discretion of the author(s).

Organization of the Manuscript

The Abstract should present the plan, rationale, and significant findings of the research. Do not use abbreviations in the abstract.

The Introduction should state the purpose of the investigation and its relation to work in the same field without extensive review of the literature.

Materials and Methods should be brief but adequate for repetition of the work. Refer to previous methods and describe only the pertinent modifications.

Results may be presented in tables or figure.

The Discussion should be concise, interpreting the data and relating it to other published works.

References should be cited in the text by a number in parentheses and listed at the end of the paper in order of citation in the text; i.e., in numerical sequence. The following are examples:

1. Bakry NMS, El-Rashidy AH, Eldefrawi AT, Eldefrawi ME. Direct actions of organophosphate anticholinesterases on nicotinic and muscarinic acetylcholine receptors. J. Biochem. Toxicol. 1988;3:235–259.

2. Tynes RE, Sabourin PJ, Hodgson E. Identification of distinct hepatic and pulmonary forms of microsomal flavin-containing monooxygenase in the mouse and rabbit. Biochem. Biophys. Res. Commun. 1985;126(3):1069–259.

3. DeBruin A. Biochemical Toxicology of Environmental Agents, 2nd repr. Amsterdam: Elsevier; 1980. 1544 p.

4. Neal RA. Microsomal metabolism of thionosulfur compounds: mechanisms and toxicological significance. In: Hodgson E, Bend JR, Philpot RM, editors. Reviews of Biochemical Toxicology. Amsterdam: Elsevier; 1980. p 131–172.

Include first and last page numbers. Accepted papers are cited as above indicating in press with the projected publishing date. For journal abbreviations, refer to the Chemical Abstracts Service Source Index (1969) and its supplements.

For the preparation of index entries for your article select Key Words under which your article should be indexed. Use the title and abstract of the article being indexed, considering carefully the words that are chosen for index listings. Limit your choices to those words which best describe the most significant findings in the paper. Avoid vague terms or those encompassing too large an area. Do not use abbreviations unless they are in general use (e.g., DDT, DNA, etc.).

Preparation of Tables

Tables should be numbered with Arabic numerals and cited consecutively in the text. Each table should be titled and typed double-spaced on a separate sheet. Sufficient experimental details (if not described in the text) should be included as a footnote using sequential lower case letters (as superscripts). Units must be indicated clearly for each of the entries in the table. Each column should carry an appropriate heading.

Define all statistical measures.

Complex tables may be submitted as "camera ready copy" for precise publication. Such tables should be typed single-spaced. Keep to a minimum the space between columns of figures. Horizontal rules above and below the column headings and at the end of the table body (i.e., before any table footnotes) should be added to the full width of the table; straddle rules should be drawn to the width of all the subcolumn heads which are below a major column heading. Do not add vertical rules in tables.

Multipliers by powers of 10 are sometimes useful. To minimize the confusion, employ decimal units (mM, nm, µL, ng, etc.) instead of exponent multipliers.


Figures must be submitted in a form suitable for reproduction with proportionate-sized lettering throughout. The maximum final printed page width in the journal is 7 inches (178 mm). If drawing to scale is not possible, please bear in mind that figures will be reduced to fit and lettering sizes should thus be scaled accordingly (a final depth of approximately 1/8", 3 mm is a good guide for lettering after reduction). Glossy black-and-white photographic prints must be supplied for all illustrations; do not send original artwork or films. Each figure must be marked clearly on the reverse (preferably on an adhesive label so that writing does not penetrate the photographic paper) with the author's name, figure number (arabic), and top of the figure. Do not mount the figures or use clips of any kind which will mark them. If multiple-part figures are used, each part must be sized proportionately to the other parts. Stereospecific illustrations must be submitted exactly as they will appear in the journal. Simple curves, e.g., dose-response, enzyme kinetics, should be reduced to a smaller size unless these are really complex. Spectra shoud be presented on a scale; that is, linear in frequency (or wave number) rather than in wavelength. In such cases indicate wavelength on a nonlinear scale at the top of the diagram (use nanometers, nm).

Figure titles and legends (containing definitions of all symbols used in the figure) should be typed double-spaced in list form on separate sheets and numbered consecutively in the sequence in which the figures are cited in the text.

Computer-generated laser-printed figures may be acceptable if printed on good quality paper and of sufficient clarity and contrast for subsequent photographic reduction.

All color figures will be reproduced in full color in the online edition of the journal at no cost to authors. Authors are requested to pay the cost of reproducing color figures in print. Authors are encouraged to submit color illustrations that highlight the text and convey essential scientific information. For best reproduction, bright, clear colors should be used. Dark colors against a dark background do not reproduce well; please place your color images against a white background wherever possible.

Chemical Formulas

Chemical formulas should be described in the text. Structural formulas, metabolic pathways and equations, and mathematical formulas should be presented for direct photographic reproductions (a stencil is suggested, or the use of transfer lettering – Chartpak, Letraset brands, for example – for a professional appearance). Computer-generated formulas printed with a laser printer may be acceptable if they are of sufficient clarity and contrast for subsequent photographic reduction.

Ionic charges should be designated as superscript, e.g., Ca 2 , Mg 2 , Na 2 . The symbol for the isotope introduced is placed in square brackets, e.g., [14-C]-aldrin, [L- methyl -14-C]-methionine, [ cis -14C]-chlordane. The symbol U indicates uniform and G general labeling, e.g., [U-14-C]-glucose or [G-14-C]-glucose (where the radioactivity is not uniformly distributed on all six carbons). The symbol indicating the configuration should precede the symbol for the isotope, e.g. [ cis -14-C]-chlordane, D-[14-C]-glucose. The abbreviation 32-P may be used for radioactive inoganic phosphate. [Refer to Biochem . J. 169:1 (1978).]

For spectrophotometric data the molar absorption coefficient should be nm -1 cm -1 .

The composition of solutions and buffers should be specified in sufficient detail to indicate the concentrations of each species.


Refer to J. Biol. Chem. 228, 6 (1963) or Abbreviations and Symbols for Chemical Names of Special Interest in Biological Chemistry; Revised Tentative Rules, 115 , 1 (1965). Also see below.

Avoid abbreviations in titles and summaries, because these are most often translated into other languages and are used by abstractors. Do not start sentences with abbreviations. Abbreviations and symbols should be used sparingly in the text and only if advantage to the reader results. They should be defined on first occurrence, with the abbreviation following in parentheses. Chemical equations, which traditionally depend on symbols, may utilize an abbreviation or symbol for a term which appears in full in the accompanying text. Trivial names are usually sufficiently short to eliminate any need to abbreviate.

For chemical terms, follow the usages given in the indexes of Chemical Abstracts [(1962) Chem. Abst . 56 ,IN-98N]. All abbreviations, symbols, and trivial Collected Tentative Rules and Recommendations of the Commission on Biochmical Nomenclature (IUPAC-IUB) (1973) are acceptable, as are those found in the following:

For stereochemistry (IUPAC Tentative Rule) (1970) J. Org. Chem . 35 , 28492867. For immunoglobulins (WHO document) (1972) Biochemistry 11, 3311–3312.


Where one or more enzymes figure prominently in a manuscript, authors should use the Recommended (Trivial) Name given by the IUB Commission on Enzyme Nomenclature: Recommendations (1972) of the International Union of Pure and Applied Chemistry and the International Union of Biochemistry (1973, Elsevier, Amsterdam). In some cases the Systematic Name or the reaction catalyzed should also be included.

Prefixes to the Names of Units

tera10 12T
giga10 9G
mega10 6M
kilo10 3k
centi10 -2c
milli10 -3m
micro10 -6µ
nano10 -9n
pico10 -12p
femto10 -15f
atto10 -18a

Units of Concentration
molar (mole/liter)M
millimolar (mmole/liter)mM (rather than 10 -3 M)
micromolar (µmole/liter)µM (rather than 10 -6 M)
nanomolarnM (not mµM)
picomolarpM (not µµM)
part per millionmg/L, mg/kg, ppm
percent% (w/v, v/v, w/w)

Other Words
logarithm (Briggsian)log
logarithm (natural)ln
standard deviation of seriesS.D.
standard error of meanS.E.

Physical and Chemical Properties
micrometer (not micron)µm (not µ)
Angstrom (0.1 nm)Å
square centimetercm 2
cubic centimetercm 3
literL (in tables only)
microliterµL (not lambda )
milligram/cm 2mg/cm 2
microgramµg (not gamma )
counts per minutecpm
revolutions per minuterpm
Svedberg unit of
   sedimentation coefficient
   (10 -13 S) S
cycle per second (Hertz)Hz
retardation factorR F
acceleration of gravityg
specific rotation|a| t lambda
sedimentation coefficients
sedimentation coefficient
    in water at 20º,
   extrapolated to zero
   concentrationS 20,w
diffusion coefficient
   (usually given in cm 2 S -1 ) D
degree Centigrade or CelsiusºC
degree absolute (Kelvin)K
equilibrium constantK
Michaelis constantK m
lethal dose/concentation-50LD-50 or LC-50

Footnotes in the text should be identified by superscript Arabic numerals and shoud be typed on a separate sheet; footnotes in the tables should be identified with superscript lower-case letters and placed at the bottom of the tables.


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