Journal of Applied Toxicology

Cover image for Vol. 37 Issue 7

Editor-in-Chief: Philip W. Harvey

Impact Factor: 2.722

ISI Journal Citation Reports © Ranking: 2015: 33/90 (Toxicology)

Online ISSN: 1099-1263

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Presented annually at the Society of Toxicology meeting, Journal of Applied Toxicology sponsors achievements in Mixture Toxicology with awards for the best student paper and the best postdoctoral paper. In 2016, our congratulations go to:

Post Doc Award: Justin Conley, USEPA, USA (left)

Student Award: Parker Duffney, Rochester University, USA (right)

SOT Mixtures Award winners 2016

 Nanotoxicology Virtual IssueZebrafish Virtual Issue 

Parabens Virtual Issue

Recently Published Articles

  1. Assessment of the lethal and sublethal effects of 20 environmental chemicals in zebrafish embryos and larvae by using OECD TG 212

    Yoshifumi Horie, Takahiro Yamagishi, Hiroko Takahashi, Youko Shintaku, Taisen Iguchi and Norihisa Tatarazako

    Version of Record online: 29 MAY 2017 | DOI: 10.1002/jat.3487

    The purpose of this study was to investigate lethal and sublethal effects of chemicals using OECD TG 212 with zebrafish, and to examine the correlation of several sublethal effects between embryo and larval stages. We found that exposure to the test chemicals at concentrations lower than the lethal concentration induced the sublethal effects. In conclusion, the environmental chemicals assessed in this study induced both lethal and sublethal effects in zebrafish embryos and larvae, as assessed by using OECD TG 212.

  2. PM2.5-induced lung inflammation in mice: Differences of inflammatory response in macrophages and type II alveolar cells

    Miao He, Takamichi Ichinose, Seiichi Yoshida, Tomohiro Ito, Cuiying He, Yasuhiro Yoshida, Keiichi Arashidani, Hirohisa Takano, Guifan Sun and Takayuki Shibamoto

    Version of Record online: 29 MAY 2017 | DOI: 10.1002/jat.3482

    Biomarkers related to inflammation and oxidative stress in vitro and in vivo experiments were investigated to clarify the PM2.5-induced lung inflammation mechanisms. This study demonstrates that macrophages may act sensitively to LPS present in PM2.5 and release pro-inflammatory mediators via the LPS/MyD88 pathway. However, type II alveolar cells may react sensitively to oxidative stress induced by PM2.5 and cause an inflammatory response. Overall, PM2.5 may cause predominantly oxidative stress-dependent inflammation rather than LPS/MyD88-dependent inflammation in the type II alveolar cell-rich murine lungs.

  3. Diethylhexyl phthalate magnifies deposition of 14C–bisphenol A in reproductive tissues of mice

    Evan D. Borman, Nicholas Vecchi, Tyler Pollock and Denys deCatanzaro

    Version of Record online: 29 MAY 2017 | DOI: 10.1002/jat.3484

    We hypothesized that two ubiquitous endocrine disruptors, bisphenol A (BPA) and diethylhexyl phthalate (DEHP), could interact in reproductive tissues. We injected DEHP into mice before giving them a food supplement containing 14C–BPA, then measured radioactivity in their tissues. DEHP increased BPA deposition in the uterus, ovaries, and blood serum of females, and in the epididymis and blood serum of males. These data are consistent with the hypothesis that DEHP competes with BPA for conjugating enzymes.

  4. Mechanism of graphene-induced cytotoxicity: Role of endonucleases

    Tariq Fahmi, La Donna Branch, Zeid A. Nima, Dae Song Jang, Alena V. Savenka, Alexandru S. Biris and Alexei G. Basnakian

    Version of Record online: 24 MAY 2017 | DOI: 10.1002/jat.3462

    This study demonstrated that pristine graphene exposed to cultured kidney tubular epithelial cells induced DNA fragmentation measured by deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, which is usually associated with cell death. The elevation of TUNEL coincided with the increased abundances of heme oxygenase-1, HSP90, caspase-3, DNase I and EndoG, and blocked by specific inhibitors to these proteins/enzymes. Therefore, caspase-mediated and caspase-independent pathways, and endonucleases DNase I and EndoG are important for graphene toxicity.

  5. Non-animal assessment of skin sensitization hazard: Is an integrated testing strategy needed, and if so what should be integrated?

    David W. Roberts and Grace Patlewicz

    Version of Record online: 24 MAY 2017 | DOI: 10.1002/jat.3479

    The three non-animal assays for skin sensitization potential that have been formally validated and regulatory adopted – the direct peptide reactivity assay (DPRA), KeratinoSens™ assay and human Cell Line Activation Test (h-CLAT) – do not represent different key events in the adverse outcome pathway but simply act as assays for the molecular initiating event, covalent modification of protein. As their inapplicability domains are not identical, combinations of assays perform better than single assays with the binary combination DPRA + h-CLAT performing best.