Identification of microRNA biomarker candidates in urine and plasma from rats with kidney or liver damage
Francis S. Wolenski, Pooja Shah, Tomoya Sano, Tadahiro Shinozawa, Hugues Bernard, Matt J. Gallacher, Shylah D. Wyllie, Georgianna Varrone, Lisa A. Cicia, Mary E. Carsillo, Craig D. Fisher, Sean E. Ottinger, Erik Koenig and Patrick J. Kirby
Version of Record online: 11 JUL 2016 | DOI: 10.1002/jat.3358
MicroRNAs (miRNA) have great potential as novel safety biomarkers. Next-generation sequencing was used to analyze changes in miRNA profiles of tissue, plasma and urine samples of rats treated with either a nephrotoxicant (cisplatin) or one of two hepatotoxicants (acetaminophen or carbon tetrachloride). Cisplatin treatment caused an elevation of miR-378a in the urine, confirming the findings of other similar studies. Treatment with either acetaminophen or carbon tetrachloride caused a serum elevation of four liver-enriched miRNAs (miR-122, −802, −31a and −365).