Journal of Applied Toxicology

Cover image for Vol. 37 Issue 4

Editor-in-Chief: Philip W. Harvey

Impact Factor: 2.722

ISI Journal Citation Reports © Ranking: 2015: 33/90 (Toxicology)

Online ISSN: 1099-1263

Author Guidelines

For additional tools visit Author Resources - an enhanced suite of online tools for Wiley Online Library journal authors, featuring Article Tracking, E-mail Publication Alerts and Customized Research Tools.

Author Guidelines

Aims and Scope

Journal of Applied Toxicology publishes peer-reviewed original reviews and hypothesis-driven research articles on mechanistic, fundamental and applied research relating to the toxicity of drugs and chemicals at the molecular, cellular, tissue, target organ and whole body level in vivo(by all routes of exposure) and in vitro / ex vivo. All aspects of toxicology are covered (including but not limited to nanotoxicology, genomics and proteomics, teratogenesis, carcinogenesis, mutagenesis, reproductive and endocrine toxicology, toxicopathology, target organ toxicity, systems toxicity (eg immunotoxicity), neurobehavioral toxicology, mechanistic studies, biochemical and molecular toxicology, novel biomarkers, pharmacokinetics/PBPK, risk assessment and environmental health studies) and emphasis is given to papers of clear application to human health and/or provide significant contributions and impact to their field.

In addition Journal of Applied Toxicology also publishes analytical toxicology, ecotoxicology (vertebrate laboratory or field studies), new techniques and method development studies, (including research on animal alternatives and in silico modelling) and applied regulatory toxicology on novel or existing drugs and chemicals, addressing novel, important or topical aspects of toxicology.

Reviews, mini-reviews and hypothesis/theoretical papers on any aspect of toxicology including individual chemicals and multidisciplinary subjects are welcomed (see specific instructions below).

High quality histopathology photomicrographs, specimen photographs and complex diagrams (including gene regulation) will be printed in color free to authors at the editors discretion.

Authors wishing to submit a manuscript on chemical weapons material's must contact the Editor-in-Chief first to discuss merit and scope.

Authors wishing to submit a manuscript on free radical mechanisms, particularly amelioration by co-administered compounds, should note that biochemical endpoints alone of a single organ/tissue response are considered not to show sufficient scientific depth. Similarly, this journal is not the place to make health claims on natural food stuffs or botanical extracts particularly in association with amelioration of free radical /ROS type mechanisms. Full chemical characterisation must be given of natural extracts/products.

Neither the editors nor the publishers accept any responsibility for any claims made concerning the properties of any drug or chemical.

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Article formats published in the Journal of Applied Toxicology

Journal of Applied Toxicology publishes Research Articles, Short Communications, Reviews, Minireviews, Hypothesis Reviews, Toxicology Updates and Correspondence. Submitted manuscripts should not have been previously published and should not be submitted for publication elsewhere while they are under consideration by Wiley. Receipt of papers will be acknowledged. Submitted material will not be returned to the author, unless specifically requested.

Research Articles. The experimental section must be precise and give all details necessary for repeating the work. Accurate and detailed descriptions must be given of the methods used. Particular attention should be given to chemicals and reagents used (supply, constitution, purity, etc) and to full descriptions of animals and their housing and environmental conditions (strain, supply, weight, caging including size and numbers per cage, ambient temperature, humidity, lighting regime, and food and water supplies). Similar detail should be provided for ex vivo and in vitro studies including derivation of tissue and cell lines, incubation and treatment procedures. See section on “Presentation of papers”.

Short Communications should not exceed two printed pages in length, including a short abstract.

Reviews deal with topics of current interest in all relevant areas of toxicology. Rather than an assemblage of detailed information with a complete literature survey, a critically selected treatment of the material is desired; unsolved problems and possible developments should also be discussed. Although review articles are generally written upon invitation of the editor, unsolicited manuscripts are also welcome provided they are in keeping with the character of the journal. Review authors should contact the Reviews Editors D. Casciano or S. Ernst or the Editor-in-Chief P.W. Harvey.

Minireviews of up to 10, 000 words are welcomed. Minireviews can be mechanism based, subject based or compound based (where the latter may include for example updates of the protein and gene targets of specific toxic agents, collation of toxicity endpoints on a compound or class, allowed environmental levels etc) provided appropriate discussion and context is given. A minireview may be a more recent or limited update of a previous substantial review.

Hypothesis Reviews or theoretical papers are also welcomed. These should be brief analyses of topical subjects, new proposed mechanisms or balanced discussion of controversies/debates in toxicological science and be approximately 4000-6000 words but can be longer. Papers based on literature mining and analyses (ie have no laboratory based in vitro or in vivo experimental data) may fit this category. Papers based on literature mining and analyses should not be designated a “Research Article”.

For Reviews, Minireviews or Hypothesis Reviews, indicate the category above the main title on the Title Page.

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Manuscript Submission

All papers must be submitted via the online system. Journal of Applied Toxicology operates an online submission and peer review system that allows authors to submit articles online and track their progress via a web interface. Please read the remainder of these instructions to authors and then click to navigate to the Journal of Applied Toxicology online submission site.

IMPORTANT: Please check whether you already have an account in the system before trying to create a new one. If you have reviewed or authored for the journal in the past year it is likely that you will have created an account.

File types. Preferred formats for the text and tables of your manuscript are .doc, .rtf, .ppt, .xls. LaTeX files may be submitted provided that an .eps or .pdf file is provided in addition to the source files. Figures may be provided in .tiff or .eps format.


NON-LATEX USERS: Editable source files must be uploaded at this stage. Tables must be on separate pages after the reference list, and not be incorporated into the main text. Figures should be uploaded as separate figure files.

LATEX USERS: For reviewing purposes you should upload a single .pdf that you have generated from your source files. You must use the File Designation "Main Document" from the dropdown box.


NON-LATEX USERS: Editable source files must be uploaded at this stage. Tables must be on separate pages after the reference list, and not be incorporated into the main text. Figures should be uploaded as separate figure files.

LATEX USERS: When submitting your revision you must still upload a single .pdf that you have generated from your now revised source files. You must use the File Designation "Main Document" from the dropdown box. In addition you must upload your TeX source files. For all your source files you must use the File Designation "Supplemental not for review". Previous versions of uploaded documents must be deleted. If your manuscript is accepted for publication we will use the files you upload to typeset your article within a totally digital workflow.

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Copyright and Permissions

If your paper is accepted, the author identified as the formal corresponding author for the paper will receive an email prompting them to login into Author Services; where via the Wiley Author Licensing Service (WALS) they will be able to complete the license agreement on behalf of all authors on the paper.

For authors signing the copyright transfer agreement

If the OnlineOpen option is not selected the corresponding author will be presented with the copyright transfer agreement (CTA) to sign. The terms and conditions of the CTA can be previewed in the samples associated with the Copyright FAQs below:

CTA Terms and Conditions

For authors choosing OnlineOpen

If the OnlineOpen option is selected the corresponding author will have a choice of the following Creative Commons License Open Access Agreements (OAA):

Creative Commons Attribution License OAA

Creative Commons Attribution Non-Commercial License OAA

Creative Commons Attribution Non-Commercial -NoDerivs License OAA

To preview the terms and conditions of these open access agreements please visit the Copyright FAQs hosted on Wiley Author Services and visit

If you select the OnlineOpen option and your research is funded by certain funders [e.g. The Wellcome Trust and members of the Research Councils UK (RCUK) or the Austrian Science Fund (FWF)] you will be given the opportunity to publish your article under a CC-BY license supporting you in complying with your Funder requirements. For more information on this policy and the Journal’s compliant self-archiving policy please visit:

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English Editing

Papers must be in English. Oxford English Dictionary or American spelling is acceptable, provided usage is consistent within the manuscript.

Manuscripts that are written in English that is ambiguous or incomprehensible, in the opinion of the Editor, will be returned to the authors with a request to resubmit once the language issues have been improved. This policy does not imply that all papers must be written in "perfect" English, whatever that may mean. Rather, the criterion will require that the intended meaning of the authors must be clearly understandable, i.e., not obscured by language problems, by referees who have agreed to review the paper.

Authors for whom English is a second language may choose to have their manuscript professionally edited before submission to improve the English. A list of independent suppliers of editing services can be found at Japanese authors can also find a list of local English improvement services at All services are paid for and arranged by the author, and use of one of these services does not guarantee acceptance or preference for publication.

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Presentation of papers

Manuscript style. Use a standard font of the 12-point type: Times, Helvetica, or Courier is preferred. Manuscripts should be single spaced. Tables must be on separate pages after the reference list, and not be incorporated into the main text. Figures should be uploaded as separate figure files.

  • Overview. The article should consist of Title page (including full title, short title, names/address/affiliations of authors, contact details, keywords); Standard Abstract plus Short Abstract (for table of contents); Introduction; Materials and Methods (with relevant subsections); Results; Discussion (Results and Discussion should not be combined); Reference List; Figure Legends; Tables; Figures. Further details are provided below. The on-line submission process also requests information contained in the paper. Check the on-line submission PDF of your article to ensure it is complete.
  • During the submission process you must enter 1) the full title 2) the short title of up to 70 characters 3) names and affiliations of all authors and 4) the full address, including email, telephone and fax of the author who is to check the proofs.
  • Include the name(s) of any sponsor(s) of the research contained in the paper, along with grant number(s).
  • Enter an abstract of no more than 250 words for all articles. Please see the guidance below on “Writing abstracts” for JAT.
  • Keywords. Authors should prepare no more than 10 keywords for their manuscript.
  • Introduction, results and discussion. These should be separate sections. The Introduction should make clear the hypothesis being examined/rationale for the study in the context of the literature. The results should describe the key findings referring to all tables and figures (statistical P values should not be repeated if given in tables or figures). The discussion should place the findings in context with the literature and highlight the advancements made by the study (e.g. to mechanistic understanding).
  • Materials and methods. Subsections should be used in the methods with headings such as Animals (see details required below); Chemicals; Experimental Design (see details required below); Treatments; Dose Administration (see details below); Tissue Preparation etc. Sufficient detail should be presented in the methods to allow independent replication of the study (previously published work can be referenced for full details but salient information should still be presented such that the article can stand alone). The source and supply of all materials used must be provided (any chemicals or extracts not obtained from internationally recognised quality controlled suppliers require full chemical analytical characterisation- see below). This includes the source/supplier of all chemicals, reagents, culture media etc.
    • For Animals - The species, strain, supply, age, sex of animals must be clearly stated together with their maintenance (including temperature, humidity, light cycle, caging and supplier/brand of food).
    • For Experimental design - The number of animals, group sizes and allocation should be clearly presented (a diagram can be used for complex designs).
    • For Dose Administration - The basis of dose selection should be stated. A brief method of dose manufacture should be stated (see also notes below on extracts and nanoparticles). Dose route of administration (eg oral gavage), dose volume (eg mL/Kg body weight) and vehicle should be stated. Dose frequency should be clearly stated (eg once per day for 28 consecutive days).
    • Similar detail should be provided for in vitro studies (eg dose concentrations, replicates etc)
  • Appropriate welfare guidelines should be stated. Note that internationally recognised standards of animal welfare are expected and should be stated over and above institutional ethical committee approval.
  • Particular attention needs to be applied to Reference Style and the quality/standard requirements for figures including chemical structures and histopathology photomicrographs and further details are given in dedicated sections below.

For extractions of natural origin (eg plant extracts) the method of extraction and full chemical analysis of the actual materials derived and used in the experiment must be given (including analytical spectra, traces etc). This applies to all chemicals not obtained from an internationally recognised quality controlled chemical supplier. Pesticides should be the pure active ingredient (technical grade) and not branded formulations.

Nanoparticles should be appropriately characterised to confirm their physico-chemical characteristics (eg shape, size distribution etc) as used in the reported experiments (the preparation of nanoparticle suspensions should be detailed).

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Writing Abstracts

An abstract is a concise summary of the whole paper, not just the conclusions. The paper should have a standard Abstract and also a Short Abstract (see below). Subsections should not be used. The standard abstract should be no more than 250 words and convey the following:

1. An introduction to the work. This should be accessible by scientists in any field and express the necessity of the experiments executed

2. Some scientific detail regarding the background to the problem

3. A summary of the main result

4. The implications of the result

5. A broader perspective of the results, once again understandable across scientific disciplines

It is crucial that the abstract convey the importance of the work and be understandable without reference to the rest of the manuscript to a multidisciplinary audience. Abstracts should not contain any citation to other published works.

Short Abstract for Table of Contents

In addition to the standard abstract, please also supply a short abstract of up to 80 words for publication in the table of contents. This should be provided immediately below the standard abstract.

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Reference Style

References References should be quoted in the text as name and year within brackets, and listed at the end of the paper alphabetically. References quoted in the text for 3 or more authors give first author only followed by et al for example Brown, Smith and Jones (2015) becomes Brown et al (2015). However, all authors should be included in the reference in the reference list at the end of the paper. All references must be complete and accurate and must use Index Medicus journal abbreviations for links to MEDLINE. To check for correct abbreviations please see If necessary, cite unpublished or personal work in the text but do not include it in the reference list. Online citations should include date of access. Where possible the DOI for the reference should be included at the end of the reference. References should be listed in the following style


Hartig S, Fries S, Balcarcel RR. 2005. Reduced mitochondrial membrane potential and metabolism correspond to acute chloroform toxicity of in vitro hepatocytes. J. Appl. Toxicol. 25 :310-317. DOI: 10.1002/jat.1067.


Magnusson B, Kligman AM. 1970. Allergic Contact Dermatitis in the Guinea Pig: Identification of Contact Allergens. Charles C. Thomas: Springfield

Chapter in Book

Luthy J, Benn M. 1980. Cyanoepithioalkanes: some chemical and toxicological studies. In Natural Sulfur Compounds: Novel Biochemical and Structural Aspects , Cavillini D, Gaull GE, Zappia V (eds). Plenum Press: New York; 381-389


The Oncology Website. [24 April 1999]

Citing EarlyView Articles

To include the DOI in a citation to an article, simply append it to the reference as in the following example:

R. K. Harris, A. Nordon, K. D. M. Harris, Rapid. Commun. Mass Spec. 2007, DOI: 10.1002/rcm.21464.

To link to an article from the author’s homepage, take the DOI (digital object identifier) and append it to "" as per following example:

DOI 10.1002/jat.1298, becomes

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Illustrations, Photomicrographs and Chemical Structures

Upload each illustration as a separate file in either .tiff or .eps format, with the figure number and the top of the figure indicated. Compound figures e.g. 1a, b, c should be uploaded as one figure. Tints are not acceptable. Lettering must be of a reasonable size that would still be clearly legible upon reduction, and consistent within each figure and set of figures. Where a key to symbols is required, please include this in the artwork itself, not in the figure legend. All illustrations must be supplied at the correct resolution:

  • Black and white and colour photos - 300 dpi
  • Graphs, drawings, etc - 800 dpi preferred; 600 dpi minimum
  • Combinations of photos and drawings (black and white and colour) - 500 dpi

Special care needs the quality of microphotographs. Before submitting microphotographs, please read the information on the Wiley Blackwell's website at:

Vector graphics (e.g. line artwork) should be saved in Encapsulated Postscript Format (EPS), and bitmap files (e.g. photographs) in Tagged Image File Format (TIFF). Line art must be scanned at a minimum of 800 dpi, photographs at a minimum of 300 dpi.

Tables should be part of the main document and should be placed after the references. If the table is created in excel the file should be uploaded separately.

Chemical structures should be prepared in ChemDraw either 80mm (one column) or 175mm (two column) widths. However, the one-column format should be used whenever possible as this allows greater flexibility in the layout of the manuscript. Use the ChemDraw Download at or use the following settings:

Drawing settingsText settings
chain angle120°fontArial
bond spacing18% of lengthsize12 pt
fixed length17 pt
bond width2 ptPreferences
line width0.75 ptunitspoints
margin width2 pttolerances5 pixels
hash spacing2.6 pt
Bold width2.6 pt

Authors using different structural drawing programs should choose settings consistent with those above. Compound numbers should be bold, but not atom labels or captions.

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Colour policy

When considered necessary by the Editors, two colour pages per article will be printed free of charge. The cost of additional colour illustrations printed in the journal will be charged to the author. If colour illustrations are supplied electronically in either TIFF or EPS format, they may be used in the PDF of the article at no cost to the author, even if this illustration was printed in black and white in the journal. The PDF will appear on the Wiley Online Library site.

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Supporting Information

Supporting Information can be a useful way for an author to include important but ancillary information with the online version of an article. Examples of Supporting Information include additional tables, data sets, figures, movie files, audio clips, 3D structures, and other related nonessential multimedia files. Supporting Information should be cited within the article text, and a descriptive legend should be included. It is published as supplied by the author, and a proof is not made available prior to publication; for these reasons, authors should provide any Supporting Information in the desired final format.

For further information on recommended file types and requirements for submission, please visit:

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Further Information

For accepted manuscripts the publisher will supply proofs to the submitting author prior to publication. This stage is to be used only to correct errors that may have been introduced during the production process. Prompt return of the corrected proofs, preferably within two days of receipt, will minimise the risk of the paper being held over to a later issue. Free access to the final PDF of the article will be available via Author Services only. Reprints can be purchased at current printing prices. There is no page charge to authors.

Manuscript accepted for publication? If so, check out our suite of tools and services for authors and sign up for:

  • Article Tracking
  • E-mail Publication Alerts
  • Personalization Tools

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Ethical Treatment of Humans and Animals

All human and animal studies must be approved by an appropriate ethics committee or review board (depending on local arrangements), and a statement to this effect should be included in the methods section, or the reasons why it was not necessary if this is the case. All clinical investigations must have been conducted according to the principles expressed in the Declaration of Helsinki (

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Toxicologists • toxicologic pathologists • environmental scientists • occupational hygienists • clinical pharmacologists • risk assessment specialists

Guidelines for Cover Submissions

If you would like to send suggestions for artwork related to your manuscript to be considered to appear on the cover of the journal, please follow these general guidelines.

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