Phytochemical Analysis

Cover image for Vol. 28 Issue 5

Editor-in-Chief: Satyajit D. Sarker

Impact Factor: 2.292

ISI Journal Citation Reports © Ranking: 2016: 36/76 (Chemistry Analytical); 45/77 (BIOCHEMICAL RESEARCH METHODS); 60/211 (Plant Sciences)

Online ISSN: 1099-1565

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Many Congratulations to Award Winners

Congratulations to Andy J. Pérez, Institute of Soil Science and Plant Cultivation, State Research Institute, Poland and Jan Šimura, Palacký University of Olomouc, Czech Republic on winning the PCA best oral presentation awards at the 2016 Trends in Natural Products Research conference, and to Yaroslav Faletrov, Belarusian State University, Belarus on winning the PCA best oral presentation award at the 23rd Conference on Isoprenoids in Minsk.

2016 Best oral presentation award

2016 Best oral presentation award

2016 Best oral presentation award

Recently Published Articles

  1. Simultaneous Qualitative and Quantitative Analyses of Triterpenoids in Ilex pubescens by Ultra-High-Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry

    Di Cao, Qing Wang, Jing Jin, Maosong Qiu, Lian Zhou, Xinghong Zhou, Hui Li and Zhongxiang Zhao

    Version of Record online: 18 SEP 2017 | DOI: 10.1002/pca.2725

    Ilex pubescens Hook et Arn. mainly contains triterpenoids that possess antithrombotic, anti-inflammatory and analgesic effects. The established UPLC-ESI-QTOF-MS method was successfully applied for simultaneous qualitative and quantitative analyses of triterpenoids in I. pubescens. A total of 33 triterpenoids were characterised, and the contents of nine triterpenoids among root, root heartwood and root bark of I. pubescens were finally determined, respectively. The results may provide a basis for holistic quality control and metabolic studies of I. pubescens.

  2. A Metabolomic Approach Applied to a Liquid Chromatography Coupled to High-Resolution Tandem Mass Spectrometry Method (HPLC-ESI-HRMS/MS): Towards the Comprehensive Evaluation of the Chemical Composition of Cannabis Medicinal Extracts

    Cinzia Citti, Umberto Maria Battisti, Daniela Braghiroli, Giuseppe Ciccarella, Martin Schmid, Maria Angela Vandelli and Giuseppe Cannazza

    Version of Record online: 15 SEP 2017 | DOI: 10.1002/pca.2722

    Thumbnail image of graphical abstract

    Untargeted metabolomics was successfully applied to unveil the main differences between cannabis based medicinal extracts obtained using two different solvents and four extraction time points for each solvent. A series of cannabinoids and other relevant small molecules, including alkaloids and amino acids, were identified among the most discriminating metabolites. Our results suggested that the main difference lies in the ratio of acid to decarboxylated cannabinoids, which dramatically influences the pharmacological activity of CMEs.

  3. Benefits and Limitations of DNA Barcoding and Metabarcoding in Herbal Product Authentication

    Ancuta Cristina Raclariu, Michael Heinrich, Mihael Cristin Ichim and Hugo de Boer

    Version of Record online: 14 SEP 2017 | DOI: 10.1002/pca.2732

    Herbal medicines play an important role globally in the health care sector and in industrialised countries they are often considered as an alternative to mono-substance medicines. Different methods have varying resolution and usefulness along the value chain of these products. DNA barcoding can be used for authenticating products based on single herbal ingredients and DNA metabarcoding for assessment of species diversity in processed products, and both methods should be used in combination with appropriate hyphenated chemical methods for quality control.

  4. The use of 1H–NMR Metabolomics to Optimise the Extraction and Preliminary Identification of Anthelmintic Products from the Leaves of Lysiloma latisiliquum

    Gloria Ivonne Hernández-Bolio, Erika Kutzner, Wolfgang Eisenreich, Juan Felipe de Jesús Torres-Acosta and Luis Manuel Peña-Rodríguez

    Version of Record online: 12 SEP 2017 | DOI: 10.1002/pca.2724

    A 1H–NMR metabolomics study of leaf extracts of Lysiloma latisiliquum, a plant with anthelmintic properties, was used to optimise the extraction and identification of the bioactive metabolites. The principal component analysis (PCA) of the 1H–NMR data allowed the identification of hydrophilic solvents as those best suited for the extraction of anthelmintics from L. latisiliquum and indicated that the bioactive metabolites are high-polarity, glycosylated products. Similarly, orthogonal projections to latent structures discriminant analysis (OPLS-DA) of the data enabled the detection of activity-related signals, assigned to the glycosylated metabolites quercitrin and arbutin.

  5. Metabolic Alterations in Two Cirsium Species Identified at Distinct Phenological Stages using UPLC-QTOF/MS

    Min-Sun Kim, Miso Nam and Geum-Sook Hwang

    Version of Record online: 12 SEP 2017 | DOI: 10.1002/pca.2716

    To characterise differences between the floral budding and full flowering stages, we determined the metabolic profile of Cirsium extracts using ultra-performance liquid chromatography (UPLC)-quadrupole time-of-flight (QTOF) mass spectrometry (MS), and the major metabolites were quantified using UPLC-QTOF/MS-multiple reaction monitoring (MRM). Phenolic acids and flavonoids of the phenylpropanoid pathway contributed to the differences between the two phenological stages. This is the first report of metabolite changes in Cirsium species at different phenological stages and will help determine the optimal harvest time for the plant in the future.