Phytochemical Analysis

Cover image for Vol. 27 Issue 5

Editor-in-Chief: Satyajit D. Sarker

Impact Factor: 2.497

ISI Journal Citation Reports © Ranking: 2015: 25/75 (Chemistry Analytical); 37/77 (BIOCHEMICAL RESEARCH METHODS); 49/209 (Plant Sciences)

Online ISSN: 1099-1565

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Many Congratulations to Award Winners

Congratulations to Yao Shen, Wageningen University, The Netherlands and Vladimir Skalicky, The Czech Republic on winning the PCA best lecture and best poster Awards at the recent Trends in Natural Product Research conference.

Yao ShenVladimir Skalicky

Recently Published Articles

  1. Field-based Evaluation of a Novel SPME-GC-MS Method for Investigation of Below-ground Interaction between Brassica Roots and Larvae of Cabbage Root Fly, Delia radicum L.

    William Deasy, Tom Shepherd, Colin J. Alexander, A. Nicholas E. Birch and K. Andrew Evans

    Version of Record online: 30 SEP 2016 | DOI: 10.1002/pca.2634

    A non-invasive method for in situ passive sampling of volatiles from plant roots using SPME in field conditions is described. SPME fibres located in pre-positioned perforated PTFE tubes were used to trap volatiles from broccoli plants before and after induction of root damage by larvae of Delia radicum. Temporal changes in the production of damage-induced sulphur containing volatiles were observed using SPME-GC-MS, showing this method to be a powerful tool for non-invasive sampling of root volatiles below ground.

  2. Needle Trap Device as a New Sampling and Preconcentration Approach for Volatile Organic Compounds of Herbal Medicines and its Application to the Analysis of Volatile Components in Viola tianschanica

    Yan Qin, Yingming Pang and Zhihong Cheng

    Version of Record online: 30 SEP 2016 | DOI: 10.1002/pca.2636

    A combined method of needle trap device (NTD) sample preparation and GC–MS was developed for qualitative analysis of volatile organic compounds (VOCs) in Viola tianschanica. A 22-gauge stainless steel, triple-bed needle packed with Tenax, Carbopack X and Carboxen 1000 sorbents exhibited good extraction performance and adequate extraction capacity for a wide range of VOCs in the herb. This NTD method could trap a wide range of VOCs including the lower and higher volatile components.

  3. In situ DART-MS as a Versatile and Rapid Dereplication Tool in Lichenology: Chemical Fingerprinting of Ophioparma ventosa

    Pierre Le Pogam, Anne-Cécile Le Lamer, Béatrice Legouin, Joël Boustie and David Rondeau

    Version of Record online: 30 SEP 2016 | DOI: 10.1002/pca.2635

    The extracellular distribution of lichen metabolites is incentive for their in situ chemical profiling. Through the specific example of Ophioparma ventosa, the present work emphasizes the potential of DART-MS to facilitate complete chemical fingerprints from unprocessed pieces of lichen. The dereplicative interest of this method is demonstrated through the detection of miriquidic acid, a rare depside so far unknown from O. ventosa. Interestingly, DART-MS also provided a first insight into the distribution of the secondary metabolites within this lichen.

  4. High Triterpenic Acids Production in Callus Cultures from Fruit Pulp of Two Apple Varieties

    Giancarlo Verardo, Andrea Gorassini, Donata Ricci and Daniele Fraternale

    Version of Record online: 30 SEP 2016 | DOI: 10.1002/pca.2638

    Callus cultures from the pulp of two varieties of apples, Golden Delicious (GD) and “Mela Rosa Marchigiana” (MRM) were obtained. The quali-quantitative analysis (ESI-MSn and GC–MS) of the methanolic extract of both cultures showed the presence of triterpenic acids. GD callus culture produced phloridzin, absent in the MRM one. Apple pulps produced higher amounts of triterpenic acids in vitro than in vivo. Copyright © 2016 John Wiley & Sons, Ltd.