Phytochemical Analysis

Cover image for Vol. 27 Issue 2

Editor-in-Chief: Satyajit D. Sarker

Impact Factor: 2.341

ISI Journal Citation Reports © Ranking: 2014: 28/74 (Chemistry Analytical); 40/79 (BIOCHEMICAL RESEARCH METHODS); 56/204 (Plant Sciences)

Online ISSN: 1099-1565

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Many Congratulations to Award Winners

Congratulations to Yao Shen, Wageningen University, The Netherlands and Vladimir Skalicky, The Czech Republic on winning the PCA best lecture and best poster Awards at the recent Trends in Natural Product Research conference.

Yao ShenVladimir Skalicky

Recently Published Articles

  1. Chemotaxonomic Studies of Nine Gentianaceae Species from Western China Based on Liquid Chromatography Tandem Mass Spectrometry and Fourier Transform Infrared Spectroscopy

    Yu Pan, Ji Zhang, Yan-Li Zhao, Yuan-Zhong Wang and Hang Jin

    Article first published online: 26 FEB 2016 | DOI: 10.1002/pca.2611

    Chemotaxonomy based on targeted and non-targeted metabolic profiling is consistent with morphological classification of the nine Gentianaceae species. However, five Gentiana species based on concentration of six common compounds were classified into three groups according to their geographical regions rather than infrageneric classification.

  2. The establishment of efficient bioconversion, extraction, and isolation processes for the production of phyllodulcin, a potential high intensity sweetener, from sweet hydrangea leaves (Hydrangea macrophyllaThunbergii) (pages 140–147)

    Chang-Hwan Jung, Yang Kim, Min-Soo Kim, Suyong Lee and Sang-Ho Yoo

    Article first published online: 20 FEB 2016 | DOI: 10.1002/pca.2609

    The ways of pre-treating hydrangea leaves were designed to maximally yield phyllodulcin via endogenous biocatalytic reaction. The greatest phyllodulcin was extracted using subcritical methanol with 3 cycles at 70°C for 5 min each. Phyllodulcin in the extract was isolated with a preparative HPLC after mixed-bed ion exchanging step. Finally, over 99% purity of phyllodulcin powder was obtained with the yield of 2.12%. Industrial utilization of phyllodulcin as potential high-intensity sweetener would be feasible due to this promising production process.

  3. Survey of pyrrolizidine alkaloids in seven varieties of Lappula squarrosa: An alternative source of heart-healthy vegetable oil (pages 133–139)

    Emmanuel Letsyo, Gerold Jerz, Peter Winterhalter, Gert Horn and Till Beuerle

    Article first published online: 20 FEB 2016 | DOI: 10.1002/pca.2608

    Seven different varieties of Lappula squarrosa were investigated to determine the potential candidate(s) for commercial seed oil cultivation, based on the content of toxic pyrrolizidine alkaloids (PAs). Flowers contained higher PA concentration, on an average, compared to other vegetative parts of the plant. In all varieties, six PAs (supinine, amabiline, intermedine, lycopsamine, 3'-acetylintermedine and unknown retronecine-type PA) were detected, with 3'-acetylintermedine dominating in most aerial parts. The PA contents of the seeds ranged from 2.88-10.36 μg PA/g d.w.

  4. A luminescence assay for natural product inhibitors of the Mycobacterium tuberculosis proteasome (pages 126–132)

    Amber Gunderwala and John Porter

    Article first published online: 18 JAN 2016 | DOI: 10.1002/pca.2607

    The Mycobacterium tuberculosis (Mtb) proteasome enhances Mtb survival. We developed and validated a luminescence assay to test natural product extracts as Mtb proteasome inhibitors. We investigated fluorescence and luminescence assays as screening methods to determine their robustness and repeatability. The fluorescence assay was subject to interference by the natural fluorescence of compounds in extracts; there was little interference in the luminescence approach. The luminescence assay is both sensitive and robust for measuring Mtb proteasome inhibition.

  5. Application of diffusion-edited and solvent suppression 1H-NMR to the direct analysis of markers in valerian-hop liquid herbal products (pages 100–106)

    Jose M. Prieto, Maria Mellinas-Gomez and Mire Zloh

    Article first published online: 13 JAN 2016 | DOI: 10.1002/pca.2603

    Direct One-dimensional diffusion-edited 1H-NMR spectroscopy (1D DOSY) successfully detected expired commercial tinctures of valerian, as well as the presence of phytomarkers from hops in valerian-hops commercial tinctures. This technique did not require evaporation and re-dissolution of the sample in deuterated solvents but only the addition of D2O and TSP to the commercial tincture. The best diagnostic signals were found at δ 4.2 (hydroxyvalerenic acid) and δ 1.5–1.8 ppm (α-acids).

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