Phytochemical Analysis

Cover image for Vol. 27 Issue 1

Editor-in-Chief: Satyajit D. Sarker

Impact Factor: 2.341

ISI Journal Citation Reports © Ranking: 2014: 28/74 (Chemistry Analytical); 40/79 (BIOCHEMICAL RESEARCH METHODS); 56/204 (Plant Sciences)

Online ISSN: 1099-1565

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Many Congratulations to Award Winners

Congratulations to Yao Shen, Wageningen University, The Netherlands and Vladimir Skalicky, The Czech Republic on winning the PCA best lecture and best poster Awards at the recent Trends in Natural Product Research conference.

Yao ShenVladimir Skalicky

Recently Published Articles

  1. A luminescence assay for natural product inhibitors of the Mycobacterium tuberculosis proteasome

    Amber Gunderwala and John Porter

    Article first published online: 18 JAN 2016 | DOI: 10.1002/pca.2607

    The Mycobacterium tuberculosis (Mtb) proteasome enhances Mtb survival. We developed and validated a luminescence assay to test natural product extracts as Mtb proteasome inhibitors. We investigated fluorescence and luminescence assays as screening methods to determine their robustness and repeatability. The fluorescence assay was subject to interference by the natural fluorescence of compounds in extracts; there was little interference in the luminescence approach. The luminescence assay is both sensitive and robust for measuring Mtb proteasome inhibition.

  2. Application of diffusion-edited and solvent suppression 1H-NMR to the direct analysis of markers in valerian-hop liquid herbal products

    Jose M. Prieto, Maria Mellinas-Gomez and Mire Zloh

    Article first published online: 13 JAN 2016 | DOI: 10.1002/pca.2603

    Direct One-dimensional diffusion-edited 1H-NMR spectroscopy (1D DOSY) successfully detected expired commercial tinctures of valerian, as well as the presence of phytomarkers from hops in valerian-hops commercial tinctures. This technique did not require evaporation and re-dissolution of the sample in deuterated solvents but only the addition of D2O and TSP to the commercial tincture. The best diagnostic signals were found at δ 4.2 (hydroxyvalerenic acid) and δ 1.5–1.8 ppm (α-acids).

  3. Flavonoids from the flowers of Impatiens glandulifera Royle isolated by high performance countercurrent chromatography

    Mariana N. Vieira, Peter Winterhalter and Gerold Jerz

    Article first published online: 11 JAN 2016 | DOI: 10.1002/pca.2606

    Impatiens glandulifera (Balsaminaceae) is one of the most important neophyte invading plant in Germany. Therefore, the aim of this work was to investigate the chemical composition of its flowers using high performance countercurrent chromatography (HPCCC). HPCCC runs lead to the isolation of dihydromyricetin, eriodictyol-7-O-glucoside, kaempferol-3-O-glucoside and kaempferol-3-O-6″-malonyl-glucoside, as well as the pre-purification of kaempferol-3-O-rhamnosyldiglucoside, quercetin-3-O-galactoside, quercetin and kaempferol in a single step. The combination of ESI-MS/MS metabolite profiling, LC-ESI-MS/MS, UV-Vis and 1D/2D-NMR data allowed the identification of the isolated compounds.

  4. Chemical characterisation of Nigerian red propolis and its biological activity against Trypanosoma Brucei

    Ruwida M. K. Omar, John Igoli, Alexander I. Gray, Godwin Unekwuojo Ebiloma, Carol Clements, James Fearnley, Ru Angeli Edrada Ebel, Tong Zhang, Harry P. De Koning and David G. Watson

    Article first published online: 11 DEC 2015 | DOI: 10.1002/pca.2605

    The proton and carbon data for the nine known compounds isolated from Nigerian propolis are shown in S1. 1H, 13C, COSY, HMBC and HMQC NMR spectra for riverinol are shown in S2. S3 shows extracted ion traces for retusapurpurins. Table S4 summarises the exact masses and retention times for the top 200 compounds by intensity in Nigerian red propolis. S5 shows some examples of the plots used to determine EC50 values of compounds tested against T.brucei.

  5. You have free access to this content
    Preparation and application of a monoclonal antibody against the isoflavone glycoside daidzin using a mannich reaction-derived hapten conjugate (pages 81–88)

    Gorawit Yusakul, Seiichi Sakamoto, Thaweesak Juengwatanatrakul, Waraporn Putalun, Hiroyuki Tanaka and Satoshi Morimoto

    Article first published online: 6 DEC 2015 | DOI: 10.1002/pca.2604

    The Mannich reaction was used to synthesize cBSA-daidzin conjugate for production of monoclonal antibody against daidzin. The icELISA showed high selectivity and acceptable sensitivity for daidzin determination (1.56–100 ng/mL) with high reproducibility. The icELISA was a reliable analytical method for daidzin in Glycine max, Pueraria lobata and Pueraria mirifica, for which daidzin recoveries from spiked samples were 98.99%–104.94%. Daidzin content of these plant-derived products determined using the icELISA were in close agreement with a HPLC-UV method.

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