Phytochemical Analysis

Cover image for Vol. 28 Issue 4

Editor-in-Chief: Satyajit D. Sarker

Impact Factor: 2.292

ISI Journal Citation Reports © Ranking: 2016: 36/76 (Chemistry Analytical); 45/77 (BIOCHEMICAL RESEARCH METHODS); 60/211 (Plant Sciences)

Online ISSN: 1099-1565

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Many Congratulations to Award Winners

Congratulations to Andy J. Pérez, Institute of Soil Science and Plant Cultivation, State Research Institute, Poland and Jan Šimura, Palacký University of Olomouc, Czech Republic on winning the PCA best oral presentation awards at the 2016 Trends in Natural Products Research conference, and to Yaroslav Faletrov, Belarusian State University, Belarus on winning the PCA best oral presentation award at the 23rd Conference on Isoprenoids in Minsk.

2016 Best oral presentation award

2016 Best oral presentation award

2016 Best oral presentation award

Recently Published Articles

  1. Qualitative Analysis of Polyphenols in Macroporous Resin Pretreated Pomegranate Husk Extract by HPLC-QTOF-MS

    Rahima Abdulla, Sanawar Mansur, Haizhong Lai, Ablikim Ubul, Guangying Sun, Guozheng Huang and Haji Akber Aisa

    Version of Record online: 23 JUN 2017 | DOI: 10.1002/pca.2695

    In this study, 35 hydrolysable tannins and 15 flavonoids as polyphenols were detected in the extract of the Punica granatum L. husk, of which 9 compounds newly identified, by separation with HPD-300 macroporous resin followed by analyses on the HPLC-QTOF/System. In addition, the accuracy and specificity of mass spectrometric detection were validated by detailed analysis of fragmentation pathways for two major polyphenolic compounds. The study provides evidence for the quality control and drug development based on the Punica granatum L. husk, a traditional herbal medicine.

  2. Structural Characterisation of Acetogenins from Annona muricata by Supercritical Fluid Chromatography Coupled to High-Resolution Tandem Mass Spectrometry

    Laurent Laboureur, Natacha Bonneau, Pierre Champy, Alain Brunelle and David Touboul

    Version of Record online: 19 JUN 2017 | DOI: 10.1002/pca.2700

    A new method for the structural elucidation of Annonaceous acetogenins was based on supercritical fluid chromatography coupled to high resolution tandem mass spectrometry, including lithium post-column cationisation. This method appeared as sensitive as classical liquid chromatography coupled to the same mass spectrometer but significantly improved the chromatographic resolution and thus allowed the annotation of a new group of isomers of montanacin-D.

  3. Metabolome Comparison of Bioactive and Inactive Rhododendron Extracts and Identification of an Antibacterial Cannabinoid(s) from Rhododendron collettianum

    Inamullah Hakeem Said, Ahmed Rezk, Ishtiaq Hussain, Anne Grimbs, Abhinandan Shrestha, Hartwig Schepker, Klaudia Brix, Matthias S. Ullrich and Nikolai Kuhnert

    Version of Record online: 13 JUN 2017 | DOI: 10.1002/pca.2694

    Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) using liquid chromatography mass spectrometry (LC-MS) data of 14 Rhododendron leaf extracts was carried out, in which an antibacterial extract was compared to a series of inactive extracts generating a hypothesis for potential bioactive compounds. Subsequent activity guided fractionation resulted in identification of a cannabinoid as bioactive constituent confirming the bioinformatics driven hypothesis. Cytotoxicity assays using mammalian cell lines, revealed that the antibacterial cannabiorcichromenic acid did not exhibit cytotoxicity at the concentrations exerting antibacterial activity.

  4. Mass Spectrometry Imaging of low Molecular Weight Compounds in Garlic (Allium sativum L.) with Gold Nanoparticle Enhanced Target

    Maria Misiorek, Justyna Sekuła and Tomasz Ruman

    Version of Record online: 13 JUN 2017 | DOI: 10.1002/pca.2696

    The distribution of organosulphur, organoselenium compounds as well as several low molecular weight compounds such as amino acids, peptides and fatty acids within sprouted garlic clove cross-section was studied using a mass spectrometry imaging (MSI) method with gold nanoparticle enhanced target (AuNPET). Ions of endogenous compounds of interest were characterised in order to assess their distribution in garlic tissue and biological function of their precursors and metabolic properties were also discussed.

  5. Phlorotannin Composition of Laminaria digitata

    Anne M. Vissers, Augusta Caligiani, Stefano Sforza, Jean-Paul Vincken and Harry Gruppen

    Version of Record online: 13 JUN 2017 | DOI: 10.1002/pca.2697

    Phlorotannins in Laminaria digitata were characterised. 13C NMR spectroscopy revealed a fucol-to-phlorethol linkage type ratio of 1:26. Quantitative 1H NMR spectroscopy allowed determination of the response factors required for colorimetric quantification by the DMBA-assay. Mass fragmentation data and 13C NMR spectroscopy (an average number of 1.5 hydrogen atoms per benzene ring) data indicated the presence of branched phlorotannin isomers. MALDI-TOF-MS analysis showed the presence of oligomeric phlorotannins up to DP27.

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