Phytochemical Analysis

Cover image for Vol. 27 Issue 3-4

Editor-in-Chief: Satyajit D. Sarker

Impact Factor: 2.497

ISI Journal Citation Reports © Ranking: 2015: 25/75 (Chemistry Analytical); 37/77 (BIOCHEMICAL RESEARCH METHODS); 49/209 (Plant Sciences)

Online ISSN: 1099-1565

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Many Congratulations to Award Winners

Congratulations to Yao Shen, Wageningen University, The Netherlands and Vladimir Skalicky, The Czech Republic on winning the PCA best lecture and best poster Awards at the recent Trends in Natural Product Research conference.

Yao ShenVladimir Skalicky

Recently Published Articles

  1. You have free access to this content
    Rapid Detection and Characterisation of Triterpene Saponins from the Root of Pulsatilla chinensis (Bunge) Regel by HPLC-ESI-QTOF-MS/MS (pages 174–183)

    Yun Ling, Zhanqing Lin, Wenjuan Zha, Tou Lian and Shanshan You

    Version of Record online: 17 JUN 2016 | DOI: 10.1002/pca.2613

    In this paper, a sensitive and selective HPLC-ESI-QTOF-MS/MS method in the negative ion mode was developed and used for rapid characterising of triterpene saponins in the root of Pulsatilla chinensis (Bunge) Regel without tedious and time-consuming isolation of pure constituents. Based on retention times, accurate mass and mass spectrometric fragmentation, 24 triterpene saponins are identified or tentatively elucidated, of which 9 triterpene saponins were not reported previously.

  2. Facile Separation of 5-O-Galloylquinic Acid from Chinese Green Tea Extract using Mesoporous Zirconium Phosphate (pages 153–157)

    Yilong Ma, Yafang Shang, Danye Zhu, Caihong Wang, Zhifeng Zhong and Ziyang Xu

    Version of Record online: 17 JUN 2016 | DOI: 10.1002/pca.2610

    A facile method for efficient separation of 5-OC-galloylquinic acid from Chinese green tea extract was developed, and the target compound was obtained by simple steps of m-ZrP adsorption, deionized water washing and 0.4% phosphoric acid solution desorption. The effects of the green tea extraction conditions, extract concentrations, and m-ZrP adsorption/desorption dynamics on the 5-O-galloylquinic acid separation were evaluated. The developed method may facilitate the separation of 5-O-galloylquinic acid from various plants.

  3. Application of a Smartphone Metabolomics Platform to the Authentication of Schisandra sinensis (pages 199–205)

    Hyuk Nam Kwon, Hong-Duc Phan, Wen Jun Xu, Yoon-Joo Ko and Sunghyouk Park

    Version of Record online: 17 JUN 2016 | DOI: 10.1002/pca.2617

    The quality of herbal medicine depends largely on the origin of the herbal material. Recent metabolomic approaches have shown great promise in authenticating the origins of herbal medicine, but sophisticated and high-cost equipment is required. Here, we report the application of a smartphone metabolomics platform as a simpler and low-cost alternative for this purpose. With a fraction of cost and greater convenience, the smartphone metabolomics platform delivered similar performance to NMR and UV-visible approaches in differentiating the origins of Schisandra sinensis from Korea and China.

  4. Antitrypanosomal Activity of a Novel Taccalonolide from the Tubers of Tacca leontopetaloides (pages 217–221)

    Vivian T. Dike, Burbwa Vihiior, Joel A. Bosha, Tung Mei Yin, Godwin Unekwuojo Ebiloma, Harry P. de Koning, John O. Igoli and Alexander I. Gray

    Version of Record online: 17 JUN 2016 | DOI: 10.1002/pca.2619

    Phytochemical analysis of the roots of Tacca leontopetaloides has led to the isolation of a novel taccalonolide and several known taccalonolides. The new taccalonolide was identified using nuclear magnetic resonance and mass spectroscopic methods as taccalonolide A-12 –propanoate. Antitrypanosomal activity assay of the new compound as well as the other isolated compounds against Trypanosoma brucei brucei Lister 427 strain, using a modified Alamar Blue assay, indicated good antiprotozoal activity with EC 50 values from 0.79 µg/ml.

  5. Direct Coupling of HPTLC with MALDI-TOF MS for Qualitative Detection of Flavonoids on Phytochemical Fingerprints (pages 222–228)

    Ivana Kroslakova, Simona Pedrussio and Evelyn Wolfram

    Version of Record online: 17 JUN 2016 | DOI: 10.1002/pca.2621

    Analysis of flavonoids is important for quality assessment of plant materials and products for many research fields, e.g. pharma, medicine, food or dietary supplements. TLC or HPTLC fingerprint analysis is commonly used for this purpose. However, successful identification of flavonoids using this method depends on the ability to visualise separated zones with reagents, and reproducibility of Rf values. Therefore, a more reliable method of analysis of their fingerprints is needed. This study demonstrates the potential of combining HPTLC with UV-MALDI-TOF MS for analysis of flavonoids.

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