Phytochemical Analysis

Cover image for Vol. 28 Issue 4

Editor-in-Chief: Satyajit D. Sarker

Impact Factor: 2.292

ISI Journal Citation Reports © Ranking: 2016: 36/76 (Chemistry Analytical); 45/77 (BIOCHEMICAL RESEARCH METHODS); 60/211 (Plant Sciences)

Online ISSN: 1099-1565

Author Guidelines

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Author Guidelines



Authors should kindly note that submission implies (i) that the content has not been published previously, in any language, in whole or in part, except as a brief abstract in the proceedings of a scientific meeting or symposium; and (ii) that the manuscript is not currently under consideration for publication elsewhere.

Once you have prepared your submission in accordance with the Guidelines, manuscripts should be submitted online at

The submission system will prompt authors to use an ORCID iD (a unique author identifier) to help distinguish their work from that of other researchers. Click here to find out more.

Click here for more details on how to use ScholarOne.

IMPORTANT: Please check whether you already have an account in the system before trying to create a new one. If you have reviewed or authored for the journal in the past year it is likely that you will have had an account created.


Preferred formats for the text and tables of your manuscript are .doc, .rtf, .ppt, .xls. LaTeX files may be submitted provided that an .eps or .pdf file is provided in addition to the source files. Figures may be provided in .tiff or .eps format.

Initial Submissions

NON-LATEX USERS: Editable source files must be uploaded at this stage. Tables must be on separate pages after the reference list, and not be incorporated into the main text. Figures should be uploaded as separate figure files.

LATEX USERS: For reviewing purposes you should upload a single .pdf that you have generated from your source files. You must use the File Designation "Main Document" from the dropdown box.

Revised Submissions

NON-LATEX USERS: Editable source files must be uploaded at this stage. Tables must be on separate pages after the reference list, and not be incorporated into the main text. Figures should be uploaded as separate figure files.

LATEX USERS: When submitting your revision you must still upload a single .pdf that you have generated from your now revised source files. You must use the File Designation "Main Document" from the dropdown box. In addition you must upload your TeX source files. For all your source files you must use the File Designation "Supplemental Material not for review". Previous versions of uploaded documents must be deleted. If your manuscript is accepted for publication we will use the files you upload to typeset your article within a totally digital workflow.

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Phytochemical Analysis (PCA) is devoted to the publication of original articles concerning the development, improvement, validation and/or extension of application of analytical methodology in the plant sciences. The spectrum of coverage is broad, encompassing methods and techniques relevant to the detection (including bio-screening), extraction, separation, purification, identification and quantification of compounds in plant biochemistry, plant cellular and molecular biology, plant biotechnology, the food sciences, agriculture and horticulture. The Journal publishes papers describing significant novelty in the analysis of whole plants (including algae), plant cells, tissues and organs, plant-derived extracts and plant products (including those which have been partially or completely refined for use in the food, agrochemical, pharmaceutical and related industries). All forms of physical, chemical, biochemical, spectroscopic, radiometric, electrometric, chromatographic, metabolomic and chemometric investigations of plant products (monomeric species as well as polymeric molecules such as nucleic acids, proteins, lipids and carbohydrates) are included within the remit of the journal. Papers dealing with novel methods relating to areas such as data handling / data mining in plant sciences will also be welcomed.

Phytochemical Analysis is intended to serve as a major resource for information on analytical and instrumental methodology in the plant sciences. Together with original articles, all of which will be both timely and of interest to a wide readership, review articles (normally no more than 30 pages) describing recent advances in specific areas of plant analysis, and mini-reviews (strictly no more than five pages) covering the highlights of any particular hot topic in phytochemical analysis will be published. Reviews and mini-reviews are by invitation only, but the Editor-in-Chief welcomes suggestions for new review topics from potential authors.

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Phytochemical Analysis publishes Research Articles, Reviews and Correspondence.

Reviews deal with topics of current interest in all relevant areas of phytochemical analysis. Rather than an assemblage of detailed information with a complete literature survey, a critically selected treatment of the material is desired; unsolved problems and possible developments should also be discussed.

Mini-reviews present concise coverage of highlights of any hot topic in phytochemical analysis. It must not exceed five pages inclusive of any tables, figures, schemes and list of references.

Although reviews and mini-reviews are generally written upon invitation of the editor, unsolicited manuscripts are also welcome provided they are in keeping with the character of the journal. Review and mini-review authors should contact the Editor-in-Chief.

The editors invite correspondence to be published, at their discretion.

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English Editing

Papers must be in English. Oxford English Dictionary or American spelling is acceptable, provided usage is consistent within the manuscript.

Manuscripts that are written in English that is ambiguous or incomprehensible, in the opinion of the Editor, will be returned to the authors with a request to resubmit once the language issues have been improved. This policy does not imply that all papers must be written in "perfect" English, whatever that may mean. Rather, the criterion will require that the intended meaning of the authors must be clearly understandable, i.e., not obscured by language problems, by referees who have agreed to review the paper.

English Checking Service for Authors from non-English speaking countries. A list of recommended English editing services is available for authors who want to have their paper checked and improved before submission. This list and further information on the service is available at: Please note that this is an optional service paid for by the author.

Manuscript style

Use a standard font of the 12-point type: Times, Helvetica, or Courier is preferred. It is not necessary to double-line space your manuscript.

Parts of the Manuscript

  • During the submission process you must enter 1) the full title 2) the short title of up to 70 characters 3) names and affiliations of all authors and 4) the full address, including email, telephone and fax of the author who is to check the proofs.
  • Include the name(s) of any sponsor(s) of the research contained in the paper, along with grant number(s).
  • Enter an abstract of no more than 250 words for all articles. Please see the guidance below on acceptable abstract writing for PCA.
  • Keywords. Authors should prepare no more than 10 keywords for their manuscript.


Articles must have a structured abstract. A structured abstract is a concise summary of the whole paper, not just the conclusions, and MUST be written using the subsections, e.g. introduction, objectives, material and methods, results, and conclusion. For further PCA guidelines for the preparation of such an Abstract, an example is available for download here.

The abstract should be no more than 250 words. It is crucial that the abstract conveys the importance of the work and be understandable without reference to the rest of the manuscript to a multidisciplinary audience. Abstracts should not contain any citation to other published works.

Short Abstract for Table of Contents

In addition to the standard abstract, please supply a short abstract of up to 80 words for publication in the graphical table of contents. [Please note that the typesetters will use the first 80 words of your full abstract if no material is supplied by the authors!]


Authors may wish to thank those who have provided assistance during the course of the described study or with the preparation of the manuscript. Written permission to use their names in the context of the paper must be obtained from all persons who are individually acknowledged in the manuscript. Acknowledgement of financial support should appear on the Title page.

Main Text: Research Articles

Research Articles have to be structured in the following way:


No sub-sections are allowed in this section. Focus on the novelty of the methodology employed and provide explicit reasons for the need to originate, develop, improve or compare methodologies (i.e. a justification of your work). Detailed reviews of the uses or biological activities of the plant material(s) studied, or of compounds derived there from, are not required. The full name (in italic font) and authority of each plant species must be provided at first mention: thereafter, plant species may be referred to in an abbreviated form unless such an abbreviation is ambiguous. Local or trivial names of plant species may be provided in the Introduction, but should not be used within the text of the manuscript as an alternative to the species name. Note that pharmaceutical preparations of the form Angelicae Radix should not be in italic font.

Use Chemical Abstracts nomenclature for chemical names and structures. Proprietary names should be accompanied by the appropriate symbol (® or ™) the first time that they are mentioned. Common acronyms for biomedical names are acceptable, but all others must be defined when first mentioned. Do not use abbreviated names or formulae (i.e. H2O, EtOAc, NaCl) for common chemicals or reagents. Non-standard units must be defined.


Second level sub-sections

Must be in bold, upper/lower case, and the text should follow on the next line.

Third level sub-sections. Must be in bold, italic, upper/lower case, and the text should follow on the same line. Provide full details of all novel techniques employed (including the names and addresses of manufacturers and suppliers of specialised chemicals or apparatus). There is no need to provide particulars of any standard procedures that have been used (a reference is sufficient): any significant modifications of the referenced method should, however, be given. State the grade of reagents employed where appropriate. Details concerning the authentication of all plant materials employed in the study are required, and voucher specimens must be available (provide the name and address of the herbarium and the reference number of each specimen). Any safety precautions regarding the experiments described must be brought to the attention of the reader. If the described research involved the use of animals or humans, a statement must be included to indicate that the work was carried out according to all local, national and international regulations and requirements, and that permission of the appropriate Ethical Committee has been obtained.


Second level sub-sections

Third level sub-sections
Sub-sections should appear on a separate line from the following text. The Results and discussion should focus on the novelty of the methodology employed and its advantages with respect to techniques previously used (if any). All novel quantitative methods described must be fully validated according to accepted International criteria and must be accompanied by full statistical data in support of such validation. Results deriving from the application of the developed methodology are welcomed but should be summarised as far as possible: such applications must not form the focus of the paper. Phytochemical Analysis does not support a Conclusions section.


Citations in the text should be given in the form of name and year, and listed in the bibliography in alphabetical (and then date) order. Where multiple citations occur at a single point in the text, they should be listed in date (and then alphabetical) order: multiple citations to the same author(s) may be listed in the form (Adam 1993, 1995; Adam et al., 1993, 1999a,b, 2004). It is the duty of the authors to ensure that all references are complete and accurate. Typically, no more than 25 references should be cited in an original research article. Authors should avoid citing lectures or abstracts from conference proceedings, papers published in journals that are not peer-reviewed, and sources that are not normally available to the reader (particularly MSc and PhD theses unless these are available on-line, in which case provide the URL).

"Unpublished results" and "Personal communications" may be cited within the text but NOT included in the bibliography. In the case of personal communications, the full name and affiliation of the communicator and the date of the communication must be quoted, and the written permission of the communicator to publish his/her name in the context of the paper must be available to the publishers. Results included in manuscripts that have been submitted to, but not accepted by, a journal may NOT be included in the bibliographic list but should be cited as "Unpublished results". Online citations should include date of access. All references must be complete and accurate. Use Index Medicus abbreviations for journal names. For correct abbreviations visit

References should be listed in the following styles:


Jaki B, Franzblau S, Pauli GF. 2004. An NMR method towards the routine chiral determination of natural products. Phytochem Anal 15: 213-219.

Xu, T., Pi, Z., Liu, S., Song, F., and Liu, Z. (2017) Chemical Profiling Combined with “Omics” Technologies (CP-Omics): a Strategy to Understand the Compatibility Mechanisms and Simplify Herb Formulas in Traditional Chinese Medicines. Phytochem. Anal., doi: 10.1002/pca.2685.


Pratt WB, Taylor P. 1990. Principles of Drug Action. The Basis of Pharmacology (3rd Edn.). Churchill Livingstone: New York; 56-78.

Chapter in Book

Bede JC, Tobe SS. 1999. Insect juvenile hormones in plants. In Studies in Natural Products Chemistry, Vol 24. Rahman A-U (Ed.). Elsevier: Amsterdam; 369-418.


European Community. 2000. Dangerous substances and preparations, marketing and regulations. Commission Directive No. 95/55/EC. European Community: Brussels. URL []; accessed May 2006.


Tables should be part of the the main document and should be placed after the references. If the table is created in excel the file should be uploaded separately.

Figures and ChemDraw Rules

Upload each figure as a separate file in either .tiff or .eps format, with the figure number and the top of the figure indicated. Compound figures e.g. 1a, b, c should be uploaded as one figure. Tints are not acceptable. Lettering must be of a reasonable size that would still be clearly legible upon reduction, and consistent within each figure and set of figures. Where a key to symbols is required, please include this in the artwork itself, not in the figure legend. All illustrations must be supplied at the correct resolution:

  • Black and white and colour photos - 300 dpi
  • Graphs, drawings, etc - 800 dpi preferred; 600 dpi minimum
  • Combinations of photos and drawings (black and white and colour) - 500 dpi

Chemical structures should be prepared in ChemDraw either 80mm (one column)or175mm (two column) widths. However, the one-column format should be used whenever possible as this allows greater flexibility in the layout of the manuscript.Use this ChemDraw Download or use the following settings:

 Drawing Settings  Text Settings 
 chain angle 120° font Arial
 bond spacing 18% of length size 12 pt
 fixed length 17 pt  
 bond width 2 pt Preferences 
 line width 0.75 pt units points
 margin width 2 pt tolerances pixels
 hash spacing 2.6 pt  
 bold width 2.6 pt  

Authors using different structural drawing programs should choose settings consistent with those above. Compound numbers should be bold, but not atom labels or captions.

Colour Figure Policy

When considered necessary by the Editors, two colour pages per article will be printed free of charge. The cost of additional colour illustrations printed in the journal will be charged to the author. If colour illustrations are supplied electronically in either TIFF or EPS format, they may be used in the PDF of the article at no cost to the author, even if this illustration was printed in black and white in the journal. The PDF will appear on the Wiley Online Library site.

Supporting Information

Supporting information is not essential to the article but provides greater depth and background and may include tables, figures, videos, data sets, etc. This material can be submitted with your manuscript, and will appear online, without editing or typesetting. Guidelines on how to prepare this material and which formats and files sizes are acceptable can be found on the Author Services website.

Wiley Author Resources

Manuscript Preparation Tips: Wiley has a range of resources for authors preparing manuscripts for submission available here. In particular, authors may benefit from referring to Wiley’s best practice tips on Writing for Search Engine Optimization.

Editing, Translation, and Formatting Support: Wiley Editing Services can greatly improve the chances of a manuscript being accepted. Offering expert help in English language editing, translation, manuscript formatting, and figure preparation, Wiley Editing Services ensures that the manuscript is ready for submission.

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Peer Review

The acceptance criteria for all papers are the quality and originality of the research and its significance to journal readership. Papers will only be sent to review if the Editor-in-Chief determines that the paper meets the appropriate quality and relevance requirements.

Wiley's policy on the confidentiality of the review process is available here.

Ethical Treatment of Humans and Animals

All human and animal studies must be approved by an appropriate ethics committee or review board (depending on local arrangements), and a statement to this effect should be included in the methods section, or the reasons why it was not necessary if this is the case. All clinical investigations must have been conducted according to the principles expressed in the Declaration of Helsinki (

Conflict of Interest

The journal requires that all authors disclose any potential sources of conflict of interest. Any interest or relationship, financial or otherwise that might be perceived as influencing an author's objectivity is considered a potential source of conflict of interest. These must be disclosed when directly relevant or directly related to the work that the authors describe in their manuscript. Potential sources of conflict of interest include, but are not limited to: patent or stock ownership, membership of a company board of directors, membership of an advisory board or committee for a company, and consultancy for or receipt of speaker's fees from a company. The existence of a conflict of interest does not preclude publication. If the authors have no conflict of interest to declare, they must also state this at submission. It is the responsibility of the corresponding author to review this policy with all authors and collectively to disclose with the submission ALL pertinent commercial and other relationships.

Publication Ethics

This journal is a member of the Committee on Publication Ethics (COPE). Note this journal uses iThenticate’s CrossCheck software to detect instances of overlapping and similar text in submitted manuscripts. Read the Top 10 Publishing Ethics Tips for Authors here. Wiley’s Publication Ethics Guidelines can be found at

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If a paper is accepted for publication, the author identified as the formal corresponding author will receive an email prompting them to log in to Author Services, where via the Wiley Author Licensing Service (WALS) they will be required to complete a copyright license agreement on behalf of all authors of the paper.

Authors may choose to publish under the terms of the journal’s standard copyright agreement, or OnlineOpen under the terms of a Creative Commons License.

General information regarding licensing and copyright is available here. To review the Creative Commons License options offered under OnlineOpen, please click here. (Note that certain funders mandate a particular type of CC license be used; to check this please click here.)

Self-Archiving Definitions and Policies: Note that the journal’s standard copyright agreement allows for self-archiving of different versions of the article under specific conditions. Please click here for more detailed information about self-archiving definitions and policies.

Open Access fees: Authors who choose to publish using OnlineOpen will be charged a fee. A list of Article Publication Charges for Wiley journals is available here.

Funder Open Access: Please click here for more information on Wiley’s compliance with specific Funder Open Access Policies.

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Accepted Article Received in Production

When an accepted article is received by Wiley's production team, the corresponding author will receive an email asking them to login or register with Wiley Author Services. The author will be asked to sign a publication license at this point.


The corresponding author will receive an email with details on how to provide proof corrections. It is therefore essential that a working email address be providing for the corresponding author.

Early View

The journal offers rapid speed to publication via Wiley’s Early View service. Early View articles are complete full-text articles published online in advance of their publication in a printed issue. Early View articles are complete and final. They have been fully reviewed, revised and edited for publication, and the authors' final corrections have been incorporated. Because they are in final form, no changes can be made after online publication. Early View articles are given a Digital Object Identifier (DOI), which allows the article to be cited and tracked before allocation to an issue. After print publication, the DOI remains valid and can continue to be used to cite and access the article.


Access and Sharing

When the article is published online: 

  • The author receives an email alert (if requested).
  • The link to the published article can be shared through social media.
  • The author will have free access to the paper (after accepting the Terms & Conditions of use, they can view the article).
  • The corresponding author and co-authors can nominate up to ten colleagues to receive a publication alert and free online access to the article.

Accessing Your Free PDF Offprint. Free access to the final PDF offprint or your article will be available via author services only. Please therefore sign up for author services if you would like to access your article PDF offprint and enjoy the many other benefits the service offers

Print copies of the article can now be ordered (instructions are sent at proofing stage or email

Promoting articles

To find out how author's can promote their articles, please click here.

Measuring the Impact of an Article

Wiley also helps authors measure the impact of their research through specialist partnerships with Kudos and Altmetric.

Author Guidelines updated 21 April 2017