Cecilia Jiménez-Sánchez, Jesús Lozano-Sánchez, Margret Brüggemann, Mariana Neves-Vieira, Miriam Rodriguez-Werner, Eva Schmalfuß, Peter Winterhalter, Antonio Segura-Carretero and Alberto Fernández-Gutiérrez
This paper compares high-speed countercurrent chromatography (HSCCC) and high-performance liquid chromatography (HPLC) in semi-preparative isolation of decarboxymethyl oleuropein aglycone (3,4-DHPEA-EDA), a bioactive secoiridoid, from extra-virgin olive oil (EVOO). An EVOO phenolic extract (EVOO-PE) was obtained by solid-phase extraction (SPE), and this extract was subjected to one run using the maximum loading capacity of each methodology without loss of resolution. For this sample set, the HSCCC method proved to be a more efficient approach regarding loading capacity, solvent consumption, and throughput compared with the HPLC method. However, the purity of the target from HPLC was superior to that of HSCCC, as the number of theoretical plates could not match that of HPLC.