Cover image for Vol. 18 Issue 19

Editor: Meghan Campbell; Editorial Board Chairs: Thomas Carell, Donald Hilvert, Barbara Imperiali

Online ISSN: 1439-7633

Associated Title(s): ChemCatChem, ChemMedChem, ChemPhysChem, ChemSusChem

Recently Published Issues

See all

Latest News

Browse more news

Find Us on FacebookFollow us on Twitter

September 22, 2017

Very Important Paper: Identification of Chimeric αβγ Diterpene Synthases Possessing both Type II Terpene Cyclase and Prenyltransferase Activities

Takaaki Mitsuhashi, Masahiro Okada, Ikuro Abe*

Prenyltransferase (PT) and terpene cyclase (TC) are key enzymes in the formation of the basic carbon skeleton of terpenoids, which constitute one of the most structurally and stereochemically diverse families of natural products. By using a genome-based approach, I. Abe (University of Tokyo, Japan) and co-workers identified two new chimeric diterpene synthases composed of three domains (α, β, and γ).These are the first enzymes possessing both Type II TC and PT activities. The α domain is responsible for the PT activity, while the βγ domains compose the Type II TC. Additionally, between the α and βγ domains, there is a characteristic linker in which minimal secondary structure is predicted. This linker does not exist in the other characterized three domain (αβγ) terpene synthases. Therefore, both the catalytic activities and protein architecture substantially differentiate these new enzymes from the previously characterized terpene synthases.

Your Comment...

[Browse more news]

Recently Published Articles

  1. High-throughput-feasible screening tool for determining enzyme stabilities against organic solvents directly from crude extracts

    Severin Wedde, Christian Kleusch, Daniel Bakonyi and Harald Gröger

    Accepted manuscript online: 12 OCT 2017 07:55AM EST | DOI: 10.1002/cbic.201700526

  2. Fluorescent Probe DCVJ Shows High Sensitivity for Characterization of Amyloid β-Peptide Early in the Lag Phase

    Dr. Sureshbabu Nagarajan and Prof. Lisa J. Lapidus

    Version of Record online: 12 OCT 2017 | DOI: 10.1002/cbic.201700387

    Thumbnail image of graphical abstract

    The sooner, the better: Whereas existing probes and techniques fail to detect oligomers at high resolution, we show that the fluorescent 9-(dicyano-vinyl)julolidine (DCVJ) binds amyloid β early aggregates. The demonstrated sensitivity of DCVJ to oligomer structure and packing provides us with a suitable probe to detect the early lag phase of amyloid assembly.

  3. Synthetic Modular Antibody Construction by Using the SpyTag/SpyCatcher Protein-Ligase System

    Dr. Md Kausar Alam, Carolina Gonzalez, Wayne Hill, Dr. Ayman El-Sayed, Dr. Humphrey Fonge, Dr. Kris Barreto and Prof. Dr. C. Ronald Geyer

    Version of Record online: 12 OCT 2017 | DOI: 10.1002/cbic.201700411

    Thumbnail image of graphical abstract

    Building devices from parts: We demonstrate a new modular approach to post-translationally assemble antibody-like “devices” from well-behaved antibody domains or “parts” using the SpyTag/SpyCatcher protein-ligase system. This system allows antibody parts to be assembled in configurations that are not possible with genetic engineering approaches.

  4. Regulating DNA Self-Assembly by DNA-Surface Interactions

    Longfei Liu, Yulin Li, Yong Wang, Jianwei Zheng and Chengde Mao

    Accepted manuscript online: 11 OCT 2017 03:02PM EST | DOI: 10.1002/cbic.201700545

  5. EroS enzyme from Aliivibrio fischeri plays Cupid to choanoflagellates

    Sean E. Rossiter and William M. Wuest

    Accepted manuscript online: 11 OCT 2017 12:25PM EST | DOI: 10.1002/cbic.201700552