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August 01, 2016
Very Important Paper: Bioorthogonal Chemical Reporters for Monitoring Unsaturated Fatty-Acylated Proteins
Emmanuelle Thinon, Avital Percher, Howard C. Hang*
Dietary unsaturated fatty acids, such as oleic acid, have been shown to be covalently incorporated into a few eukaryotic proteins but the generality and diversity of unsaturated fatty acid modifications was not yet known. Now, H.C. Hang (The Rockefeller University, USA) and co-workers synthesized unsaturated fatty acid reporters that effectively induced lipid droplets and were site-specifically incorporated onto known fatty-acylated proteins. Quantitative proteomic analysis revealed that unsaturated fatty acids in mammalian cells modify similar protein targets to saturated fatty acids, which suggests that unsaturated fatty acids may directly modify many proteins to exert their unique and often beneficial physiological effects in vivo.
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