Cover image for Vol. 16 Issue 7

Editor: Peter Gölitz; Editorial Board Chairs: Thomas Carell, Donald Hilvert, Barbara Imperiali

Online ISSN: 1439-7633

Associated Title(s): ChemCatChem, ChemMedChem, ChemPhysChem, ChemSusChem

Recently Published Issues

See all

Latest News

Browse more news

February 09, 2015

Very Important Paper: Establishing the Stability and Reversibility of Protein Pyrophosphorylation with Synthetic Peptides

Lisa M. Yates and Dorothea Fiedler*

Key factors in elucidating the biological relevance of novel post-translational modifications are to understand their chemical and biochemical stability as well as their reversibility in a cellular context. To evaluate these factors for protein pyrophosphorylation (a currently poorly characterized modification) Yates and Fiedler (Princeton University) used a series of synthetic pyrophosphopeptides to demonstrate that the pyrophosphate moiety is generally chemically inert but can be rapidly removed by alkaline phosphatases in vitro. Most importantly, they observed enzyme-dependent depyrophosphorylation of the synthetic pyrophosphopeptides in mammalian and yeast cell lysates. These findings thus provide the initial evidence for the reversibility of pyrophosphorylation and highlight the potential impact of this modification on cellular signaling networks.

Your Comment...

[Browse more news]

Recently Published Articles

  1. Enhancing Antibody Response against Small Molecular Hapten with Tobacco Mosaic Virus as a Polyvalent Carrier

    Xia Zhao, Limin Chen, Jittima Amie Luckanagul, Xiaolei Zhang, Yuan Lin and Qian Wang

    Accepted manuscript online: 24 APR 2015 06:21AM EST | DOI: 10.1002/cbic.201500028

  2. Activity Fed Translation Assay "AFT", a new high throughput screening strategy for enzymes in droplets

    Patrice Soumillion, Gabrielle Woronoff, Andrew Griffiths, Julia Wessel, Michael Ryckelynck and Olivier Schicke

    Accepted manuscript online: 24 APR 2015 06:21AM EST | DOI: 10.1002/cbic.201500087

  3. Genetic Incorporation of Nε-formyllysine, a Novel Histone Posttranslational Modification

    Tianyuan Wang, Qing Zhou, Fahui Li, Yang Yu, Xuebin Yin and Jiangyun Wang

    Accepted manuscript online: 24 APR 2015 06:21AM EST | DOI: 10.1002/cbic.201500170

  4. Exploring Bacterial Heparinase II Activities with Defined Substrates

    Lisa Bohlmann, Dr. Chih-Wei Chang, Prof. Ifor Beacham and Prof. Dr. Mark von Itzstein

    Article first published online: 23 APR 2015 | DOI: 10.1002/cbic.201500081

    Thumbnail image of graphical abstract

    Heparinases cleave heparin and heparan sulfate (HS) by an elimination mechanism and are important biologics for anticoagulation drug production and for heparin and HS characterisation. Defined HS oligosaccharides were exploited to study the substrate specificity of heparinase II from two different microbial organisms.

  5. Functional Characterization of Different ORFs Including Luciferase-Like Monooxygenase Genes from the Mensacarcin Gene Cluster

    Sarah Maier, Tanja Heitzler, Dr. Katharina Asmus, Dr. Elke Brötz, Dr. Uwe Hardter, Katharina Hesselbach, Dr. Thomas Paululat and Prof. Dr. Andreas Bechthold

    Article first published online: 23 APR 2015 | DOI: 10.1002/cbic.201500048

    Thumbnail image of graphical abstract

    MsnO2, MsnO4, and MsnO8: We elucidated the components of the Streptomyces bottropensis mensacarcin biosynthesis gene cluster by selective deletion and complementation in a heterologous expression system. Three luciferase-like monooxygenases are involved in later steps of mensacarcin biosynthesis.