ChemBioChem

Cover image for Vol. 18 Issue 4

Editor: Meghan Campbell; Editorial Board Chairs: Thomas Carell, Donald Hilvert, Barbara Imperiali

Online ISSN: 1439-7633

Associated Title(s): ChemCatChem, ChemMedChem, ChemPhysChem, ChemSusChem

Recently Published Issues

See all

Latest News

Browse more news

December 19, 2016

Very Important Paper: A Bifunctional Amino Acid Enables Both Covalent Chemical Capture and Isolation of in Vivo Protein--Protein Interactions

Cassandra M. Joiner+, Meghan E. Breen+, James Clayton, and Anna K. Mapp*

In vivo covalent chemical capture by using photo-activatable unnatural amino acids is a powerful tool for the identification of transient protein--protein interactions (PPIs) in their native environment, however, the isolation and characterization of the crosslinked complexes can be challenging. Now, A.K. Mapp (University of Michigan, U.S.A.) and co-workers report a strategy using the bifunctional unnatural amino acid, BPKyne, to capture and directly label transient PPIs in their native environment. Functionalization after crosslinking with a biotin--azide probe enabled the isolation of transcriptional protein complexes from yeast cells. BPKyne expands the toolbox for the discovery of novel PPIs in live cells and can be extended to other biological systems.

Your Comment...

[Browse more news]

Recently Published Articles

  1. Synthetic Biomarker Design Using Analyte-Responsive Acetaminophen

    Tatsuya Nishihara, Joe Inoue, Sho Tabata, Shinnosuke Murakami, Takamasa Ishikawa, Natsumi Saito, Shinji Fukuda, Masaru Tomita and Tomoyoshi Soga

    Accepted manuscript online: 25 FEB 2017 12:20AM EST | DOI: 10.1002/cbic.201700023

  2. P450 BM3-Catalyzed Regio- and Stereoselective Hydroxylation Aiming at the Synthesis of Phthalides and Isocoumarins

    Claudia Holec, Ute Hartrampf, Dr. Katharina Neufeld and Prof. Dr. Jörg Pietruszka

    Version of Record online: 24 FEB 2017 | DOI: 10.1002/cbic.201600685

    Thumbnail image of graphical abstract

    Finding the best catalyst: A set of P450 BM3 variants was tested for stereoselective hydroxylation of benzoic acid derivatives, with the goal of synthesizing phthalides and isocoumarins. We obtained variants giving access to R- and S-configured products constituting a new route to a class of compounds that are valuable synthons for a variety of natural products.

  3. Maximizing Output in RNA-Programmed Peptidyl-Transfer Reactions

    Dr. Margherita Di Pisa, Anett Hauser and Prof. Dr. Oliver Seitz

    Version of Record online: 24 FEB 2017 | DOI: 10.1002/cbic.201600687

    Thumbnail image of graphical abstract

    How to get many products from a few instructors: We investigated the RNA-templated transfer reaction between two peptide–PNA conjugates based on native chemical ligation. Thioester reactivity, template architecture, affinity, but not peptide length are key to high-performance peptidyl transfer on RNA.

  4. A QCM-D and SAXS Study of the Interaction of Functionalised Lyotropic Liquid Crystalline Lipid Nanoparticles with siRNA

    Behnoosh Tajik-Ahmadabad, Adam Mechler, Benjamin W Muir, Keith McLean, Tracey M Hinton, Frances Separovic and Anastasios Polyzos

    Accepted manuscript online: 23 FEB 2017 10:24PM EST | DOI: 10.1002/cbic.201600613

  5. Metabolic chemical reporters of glycans exhibit cell-type selective metabolism and glycoprotein labeling

    Anna R Batt, Balyn W Zaro, Marisol X Navarro and Matthew Robert Pratt

    Accepted manuscript online: 23 FEB 2017 04:20PM EST | DOI: 10.1002/cbic.201700020

SEARCH

SEARCH BY CITATION