ChemBioChem

Cover image for Vol. 15 Issue 16

Editor: Peter Gölitz; Editorial Board Chairs: Thomas Carell, Donald Hilvert, Barbara Imperiali

Online ISSN: 1439-7633

Associated Title(s): ChemCatChem, ChemMedChem, ChemPhysChem, ChemSusChem

Recently Published Issues

See all

Latest News

Browse more news

July 03, 2014

Very Important Paper: Computational Library Design for Increasing Haloalkane Dehalogenase Stability

Robert J. Floor, Hein J. Wijma, Dana I. Colpa, Aline Ramos-Silva, Peter A. Jekel, Wiktor Szymański, Ben L. Feringa, Siewert J. Marrink, Dick B. Janssen*

Enzyme stability is of key importance for the development of biocatalytic processes. A new strategy termed "framework for rapid enzyme stabilization by computation" (FRESCO) was applied by Dick B. Janssen (University of Groningen, The Netherlands) and co-workers towards stabilizing the enzyme, haloalkane dehalogenase. Using computational design methods, a small mutant library was obtained from which a set of stabilizing mutations were discovered. The best mutations were combined, providing an enzyme that was more thermostable and resistant to high concentrations of organic co-solvent. The latter made it possible to use this enzyme for dehalogenation and kinetic resolution of hydrophobic substrates that are only highly soluble in the presence of organic co-solvents.

Your Comment...

[Browse more news]

Recently Published Articles

  1. EMBO Conference Series: Chemical Biology 2014

    Dr. Eileen J. Kennedy

    Article first published online: 16 OCT 2014 | DOI: 10.1002/cbic.201402527

    Thumbnail image of graphical abstract

    Around 300 people from 18 countries took part in the fourth biennial Chemical Biology conference at The European Molecular Biology Laboratory (EMBL) in Heidelberg, from August 20 to 23, 2014. Many advances in the field of chemical biology were presented in talks and poster sessions. Picture: Petra Riedinger (EMBL).

  2. Modular Polyketide Synthases (PKSs): A New Model Fits All?

    Alexander Rittner and Martin Grininger

    Article first published online: 16 OCT 2014 | DOI: 10.1002/cbic.201402432

    Thumbnail image of graphical abstract

    Step-by-step to great diversity: With the potential to synthesize multi-millions of bioactive compounds, modular polyketide synthases (PKSs) are of great importance. In this Highlight, new developments in the understanding of the structure and function of these proteins are reviewed.

  3. High-Fidelity Recognition of RNA: Solution Structure of a DNA:RNA Hybrid Duplex with a Molecular Cap

    Claudia Gerlach, Dr. Birgit Claasen and Prof. Clemens Richert

    Article first published online: 16 OCT 2014 | DOI: 10.1002/cbic.201402409

    Thumbnail image of graphical abstract

    A bridge to RNA: The solution structure of a DNA:RNA hairpin duplex bridged by a peptidyl-linked quinolone shows how the affinity and sequence select- ivity of probes for RNA targets can be increased, with canonical base pairing being favored over hard-to-suppress mismatched base combinations such as T:G and A:A.

  4. Chemistry, Properties, and in Vitro and in Vivo Applications of 2′-O-Methoxyethyl-4′-thioRNA, a Novel Hybrid Type of Chemically Modified RNA

    Yota Saito, Yosuke Hashimoto, Mai Arai, Noriko Tarashima, Tadashi Miyazawa, Kazuya Miki, Dr. Mayumi Takahashi, Prof. Dr. Kazuhiro Furukawa, Prof. Dr. Naoshi Yamazaki, Prof. Dr. Akira Matsuda, Prof. Dr. Tatsuhiro Ishida and Prof. Dr. Noriaki Minakawa

    Article first published online: 14 OCT 2014 | DOI: 10.1002/cbic.201402398

    Thumbnail image of graphical abstract

    New RNA: A novel hybrid type of chemically modified RNA was developed, between 2′-O-methoxyethylRNA (MOERNA) and 4′-thioRNA (SRNA). The resulting 2′-O-methoxyethyl-4′-thioRNA (MOE-SRNA) showed potent anti-microRNA activity not only in vitro but also in vivo.

  5. Covalent Inhibition of New Delhi Metallo-β-Lactamase-1 (NDM-1) by Cefaclor

    Dr. Pei W. Thomas, Michael Cammarata, Dr. Jennifer S. Brodbelt and Dr. Walter Fast

    Article first published online: 10 OCT 2014 | DOI: 10.1002/cbic.201402268

    Thumbnail image of graphical abstract

    Finding a handhold: Due to mechanistic differences, using inactivators of serine-β-lactamases to inhibit metallo-β-lactamases is like trying to shake hands with an empty glove. Here, the drug cefaclor was shown to covalently inactivate New Delhi metallo-β-lactamase-1 (NDM-1), identifying Lys211 as a “handhold” for covalent inhibitor design.

SEARCH

SEARCH BY CITATION