Cover image for Vol. 16 Issue 8

Editor: Peter Gölitz; Editorial Board Chairs: Thomas Carell, Donald Hilvert, Barbara Imperiali

Online ISSN: 1439-7633

Associated Title(s): ChemCatChem, ChemMedChem, ChemPhysChem, ChemSusChem

Recently Published Issues

See all

Latest News

Browse more news

May 28, 2015

Very Important Paper: Evaluating Ketoreductase Exchanges as a Means of Rationally Altering Polyketide Stereochemistry

Thibault Annaval, Cédric Paris, Peter F. Leadlay, Christophe Jacob,* Kira J. Weissman*

Reduced polyketide secondary metabolites exhibit a wide range of useful biological properties, including antibiotic and anti-cancer activities. There is great interest in precisely tailoring these structures in order to optimize their pharmaceutical properties. One strategy to create analogues is by genetic engineering of the underlying biosynthetic machinery, the polyketide synthases (PKSs). PKSs are modular multienzymes composed of repeated sets of catalytic and carrier protein domains, each of which performs a specific task in the biosynthesis. Now, a team lead by C. Jacob and K. J. Weissman (Université de Lorraine, France) have identified the first ketoreductase domains which, when introduced into a model PKS, allow for the efficient alteration of the stereochemistry of a methyl group on the corresponding product.

Your Comment...

[Browse more news]

Recently Published Articles

  1. Conversion of a Mono- and Diacylglycerol Lipase into a Triacylglycerol Lipase by Protein Engineering

    Dr. Dongming Lan, Dr. Grzegorz Maria Popowicz, Dr. Ioannis V. Pavlidis, Pengfei Zhou, Prof. Dr. Uwe T. Bornscheuer and Prof. Dr. Yonghua Wang

    Article first published online: 29 MAY 2015 | DOI: 10.1002/cbic.201500163

    Thumbnail image of graphical abstract

    A mono- and diglyceride lipase was engineered to a triacylglyceride lipase by introducing a single point mutation (Q282L). The variant has broad substrate specificity on triacylglycerides. The results indicate that the main reason that the wild-type enzyme does not accept triacylglycerides is not their bulkiness, but specific hydrophobic interactions.

  2. Tuning the pH-response of i-motif DNA oligonucleotides

    Laurie Lannes, Saheli Halder, Yamuna Krishnan and Harald Schwalbe

    Accepted manuscript online: 28 MAY 2015 09:17AM EST | DOI: 10.1002/cbic.201500182

  3. Activity-Based Protein Profiling of Rhomboid Proteases in Liposomes

    Eliane V Wolf, Martin Seybold, Romana Hadravova, Kvido Strisovsky and Steven Verhelst

    Accepted manuscript online: 28 MAY 2015 09:12AM EST | DOI: 10.1002/cbic.201500213

  4. A rapid and high-throughput assay for the estimation of conversions of ene-reductases-catalysed reactions

    Maria Chiara Forchin, Michele Crotti, Francesco G. Gatti, Fabio Parmeggiani, Elisabetta Brenna and Daniela Monti

    Accepted manuscript online: 28 MAY 2015 09:12AM EST | DOI: 10.1002/cbic.201500219