Cover image for Vol. 17 Issue 3

Editor: Meghan Campbell; Editorial Board Chairs: Thomas Carell, Donald Hilvert, Barbara Imperiali

Online ISSN: 1439-7633

Associated Title(s): ChemCatChem, ChemMedChem, ChemPhysChem, ChemSusChem

Recently Published Issues

See all

Latest News

Browse more news

January 27, 2016

Very Important Paper: Enhancing DNA Crystal Durability through Chemical Crosslinking

Diana Zhang & Paul J. Paukstelis*

Three-dimensional DNA crystals are promising materials for nanotechnology applications. However, these applications may be limited by features inherent to the DNA lattice, including low thermal stability, high cation requirements, and nuclease sensitivity. To overcome these problems D. Zhang & P.J. Paukstelis (University of Maryland) have developed a post-crystallization crosslinking step that enhances the durability of a model DNA crystal lattice. Crystals crosslinked with nornitrogen mustard show enhanced thermal stability, decreased divalent cation requirements, and almost complete resistance to DNase I. This method for stabilizing DNA crystals may be applicable to other DNA constructs and ultimately improve the usefulness of DNA nanoarchitectures.

Your Comment...

[Browse more news]

Recently Published Articles

  1. Chemical Genetic Approaches for the Investigation of Neutral Lipid Metabolism

    Carina Doler, Dr. Martina Schweiger, Prof. Dr. Robert Zimmermann and Prof. Dr. Rolf Breinbauer

    Article first published online: 5 FEB 2016 | DOI: 10.1002/cbic.201500501

    Thumbnail image of graphical abstract

    Blocking lipid-metabolizing enzymes: Lipid-metabolizing enzymes represent logical targets for the treatment of dyslipidemia, a major risk factor for type 2 diabetes, atherosclerosis, and other disorders. In this review we summarize the current state of available inhibitors targeting key proteins in neutral lipid metabolism, with a focus on metabolic lipases, acyltransferases, and fatty-acid-binding proteins.

  2. Investigating Deformylase and Deacylase Activity of Mammalian and Bacterial Sirtuins

    Julian Seidel, Dr. Cordula Klockenbusch and Prof. Dr. Dirk Schwarzer

    Article first published online: 5 FEB 2016 | DOI: 10.1002/cbic.201500611

    Thumbnail image of graphical abstract

    Restoring lysine: Sirtuins catalyze the removal of enzymatic and nonenzymatic lysine acyl-modifications from proteins. We established a substrate scaffold to analyze sirtuin activity with a broad range of modifications. The sole sirtuin of B. subtilis, SrtN, is an efficient lysine-deformylase and can erase a broad range of lysine acyl modifications.

  3. Identification of Sequence Specificity of 5-Methylcytosine Oxidation by Tet1 Protein with High-Throughput Sequencing

    Seiichiro Kizaki, Dr. Anandhakumar Chandran and Prof. Dr. Hiroshi Sugiyama

    Article first published online: 5 FEB 2016 | DOI: 10.1002/cbic.201500646

    Thumbnail image of graphical abstract

    Oxidation of 5-methylcytosine: Tet family proteins oxidize 5-methylcytosine (mC) to 5-hydroxymethylcytosine (hmC). The sequence specificity of mC oxidation by Tet1 protein was examined with random DNA pool and high-throughput sequencing. The sequence mCpG was identified as highly reactive for Tet1.

  4. Mutating a Highly Conserved Residue in Diverse Cytochrome P450s Facilitates Diastereoselective Olefin Cyclopropanation

    Joshua G. Gober, Amy E. Rydeen, Evan J. Gibson-O'Grady, Janelle B. Leuthaeuser, Prof. Dr. Jacquelyn S. Fetrow and Prof. Dr. Eric M. Brustad

    Article first published online: 4 FEB 2016 | DOI: 10.1002/cbic.201500624

    Thumbnail image of graphical abstract

    Enzymatic cyclopropanation: Mutation of a highly conserved active site residue in a small panel of diverse cytochrome P450s allows for the rapid identification of trans- and cis-selective enzymes for the carbenoid-mediated cyclopropanation of styrenes.

  5. Fluorescent Dansyl-Guanosine Conjugates that Bind c-MYC Promoter G-Quadruplex and Downregulate c-MYC Expression

    Y. Pavan Kumar, Puja Saha, Dhurjhoti Saha, Irene Bessi, Prof. Dr. Harald Schwalbe, Dr. Shantanu Chowdhury and Prof. Dr. Jyotirmayee Dash

    Article first published online: 4 FEB 2016 | DOI: 10.1002/cbic.201500631

    Thumbnail image of graphical abstract

    Transcriptional modulator! Dansyl-guanosine conjugates synthesised by click chemistry can be used as fluorescent markers for selective visualisation of c-MYC G-quadruplex over duplex DNA by gel electrophoresis and can modulate the c-MYC gene transcription in cancer cells. The binding affinities of these ligands to c-MYC quadruplex and their anticancer properties can be controlled by modification of the “clickable” building blocks.