Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
July 03, 2014
Very Important Paper: Computational Library Design for Increasing Haloalkane Dehalogenase Stability
Robert J. Floor, Hein J. Wijma, Dana I. Colpa, Aline Ramos-Silva, Peter A. Jekel, Wiktor Szymański, Ben L. Feringa, Siewert J. Marrink, Dick B. Janssen*
Enzyme stability is of key importance for the development of biocatalytic processes. A new strategy termed "framework for rapid enzyme stabilization by computation" (FRESCO) was applied by Dick B. Janssen (University of Groningen, The Netherlands) and co-workers towards stabilizing the enzyme, haloalkane dehalogenase. Using computational design methods, a small mutant library was obtained from which a set of stabilizing mutations were discovered. The best mutations were combined, providing an enzyme that was more thermostable and resistant to high concentrations of organic co-solvent. The latter made it possible to use this enzyme for dehalogenation and kinetic resolution of hydrophobic substrates that are only highly soluble in the presence of organic co-solvents.
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