ChemBioChem

Cover image for Vol. 18 Issue 24

Editor: Ruben Ragg; Editorial Board Chairs: Thomas Carell, Donald Hilvert, Barbara Imperiali

Online ISSN: 1439-7633

Associated Title(s): ChemCatChem, ChemMedChem, ChemPhysChem, ChemSusChem

Recently Published Issues

See all

Latest News

Browse more news

Find Us on FacebookFollow us on Twitter

December 14, 2017

Very Important Paper: Kinetic Analysis of PRMT1 Reveals Multifactorial Processivity and a Sequential Ordered Mechanism

Jennifer I. Brown, Timo Koopmans, Jolinde van Strien, Nathaniel I. Martin,* Adam Frankel*

Very Important Paper: Kinetic Analysis of PRMT1 Reveals Multifactorial Processivity and a Sequential Ordered Mechanism Jennifer I. Brown, Timo Koopmans, Jolinde van Strien, Nathaniel I. Martin,* Adam Frankel*Protein arginine N-methyltransferases (PRMTs) are mechanistically complex. Published reports indicate that PRMTs demonstrate variable dimethylation processivity. Results also dispute whether these enzymes bind their target and cofactor substrates randomly or sequentially. Here, N.I. Martin (University of Utrecht, Netherlands), A. Frankel (University of British Columbia, Canada) and co-workers offer a comprehensive literature review and propose explanations for the disparities within the field. They demonstrate that PRMT1 processivity depends on both cofactor and enzyme concentration, indicating that experimental design profoundly affects substrate dimethylation. Furthermore, they show that PRMT1 binds its substrates using a sequential ordered Bi--Bi mechanism. Considering the structural and functional conservation among PRMTs, these insights likely extend throughout the PRMT family.

Your Comment...

[Browse more news]

Recently Published Articles

  1. Immobilization of Superoxide Dismutase on Polyelectrolyte-Functionalized Titania Nanosheets

    Dr. Paul Rouster, Marko Pavlovic and Dr. Istvan Szilagyi

    Version of Record online: 18 DEC 2017 | DOI: 10.1002/cbic.201700502

    Thumbnail image of graphical abstract

    New SOD: Superoxide dismutase (SOD) is successfully immobilized on titania nanosheets (TNS) functionalized with the poly(diallyldimethylammonium chloride) (PDADMAC) polyelectrolyte. TNS–PDADMAC–SOD shows excellent superoxide radical anion scavenging activity and is a promising candidate for applications in which suspensions with antioxidant activity are required in manufacturing processes.

  2. Expanding the Scope of Sortase-Mediated Ligations by Using Sortase Homologues

    Keyvan D. Nikghalb, Nicholas M. Horvath, Jesse L. Prelesnik, Orion G. B. Banks, Pavel A. Filipov, R. David Row, Travis J. Roark and Prof. John M. Antos

    Version of Record online: 18 DEC 2017 | DOI: 10.1002/cbic.201700517

    Thumbnail image of graphical abstract

    Sortase substrates: The in vitro substrate preferences of eight sortase A homologues were evaluated, revealing sortases capable of recognizing multiple sequences in addition to the canonical LPXTG motif. These promiscuous enzymes provide new options for protein modification by using sortase-based approaches.

  3. Improvement in the Thermostability of a β-Amino Acid Converting ω-Transaminase by Using FoldX

    Oliver Buß, Delphine Muller, Sven Jager, Dr. Jens Rudat and Dr. Kersten S. Rabe

    Version of Record online: 18 DEC 2017 | DOI: 10.1002/cbic.201700467

    Thumbnail image of graphical abstract

    On the basis of energy calculations using FoldX, a β-amino acid converting ω-transaminase is successfully engineered for higher temperature stability and enzymatic activity, enabling the use of higher process temperatures. Additionally, we analyze the molecular basis of the stabilization of the best variant and of the wild-type by using molecular dynamics simulations.

  4. New WS9326A Derivatives and One New Annimycin Derivative with Antimalarial Activity are Produced by Streptomyces asterosporus DSM 41452 and Its Mutant

    Songya Zhang, Jing Zhu, Prof. Dr. David L. Zechel, Dr. Claudia Jessen-Trefzer, Dr. Richard T. Eastman, Dr. Thomas Paululat and Prof. Dr. Andreas Bechthold

    Version of Record online: 18 DEC 2017 | DOI: 10.1002/cbic.201700428

    Thumbnail image of graphical abstract

    The WS9326A biosynthetic gene cluster in S. asterosporus DSM 41452 is identified through bioinformatic analysis and mutagenesis. Analysis of the cluster reveals the SAS16 gene encoding a cytochrome P450 monooxygenase that is involved in the biosynthesis of the (E)-2,3-dehydrotyrosine residue in WS9326As. Surprisingly, one mutant of S. asterosporus DSM 41452 produces a new annimycin derivative with antimalarial activity.

  5. Improved Enantioselectivity of Subtilisin Carlsberg towards Secondary Alcohols by Protein Engineering

    Robin Dorau, Tamás Görbe and Dr. Maria Svedendahl Humble

    Version of Record online: 18 DEC 2017 | DOI: 10.1002/cbic.201700408

    Thumbnail image of graphical abstract

    An acyl in the hole: The performance of the serine protease subtilisin Carlsberg (SC) is improved towards transacylation reactions by using secondary alcohols in THF. By enzyme immobilization, the enantioselectivity of SC is increased tenfold. By further protein engineering, an enzyme variant (G165L/M221F) shows increased conversion, enantioselectivity (E>100), substrate scope, and stability in THF.

SEARCH

SEARCH BY CITATION