© WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
January 27, 2016
Very Important Paper: Enhancing DNA Crystal Durability through Chemical Crosslinking
Diana Zhang & Paul J. Paukstelis*
Three-dimensional DNA crystals are promising materials for nanotechnology applications. However, these applications may be limited by features inherent to the DNA lattice, including low thermal stability, high cation requirements, and nuclease sensitivity. To overcome these problems D. Zhang & P.J. Paukstelis (University of Maryland) have developed a post-crystallization crosslinking step that enhances the durability of a model DNA crystal lattice. Crystals crosslinked with nornitrogen mustard show enhanced thermal stability, decreased divalent cation requirements, and almost complete resistance to DNase I. This method for stabilizing DNA crystals may be applicable to other DNA constructs and ultimately improve the usefulness of DNA nanoarchitectures.
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