Cover image for Vol. 18 Issue 4

Editor: Meghan Campbell; Editorial Board Chairs: Thomas Carell, Donald Hilvert, Barbara Imperiali

Online ISSN: 1439-7633

Associated Title(s): ChemCatChem, ChemMedChem, ChemPhysChem, ChemSusChem

Recently Published Issues

See all

Latest News

Browse more news

December 19, 2016

Very Important Paper: A Bifunctional Amino Acid Enables Both Covalent Chemical Capture and Isolation of in Vivo Protein--Protein Interactions

Cassandra M. Joiner+, Meghan E. Breen+, James Clayton, and Anna K. Mapp*

In vivo covalent chemical capture by using photo-activatable unnatural amino acids is a powerful tool for the identification of transient protein--protein interactions (PPIs) in their native environment, however, the isolation and characterization of the crosslinked complexes can be challenging. Now, A.K. Mapp (University of Michigan, U.S.A.) and co-workers report a strategy using the bifunctional unnatural amino acid, BPKyne, to capture and directly label transient PPIs in their native environment. Functionalization after crosslinking with a biotin--azide probe enabled the isolation of transcriptional protein complexes from yeast cells. BPKyne expands the toolbox for the discovery of novel PPIs in live cells and can be extended to other biological systems.

Your Comment...

[Browse more news]

Recently Published Articles

  1. On the Influence of the Protonation States of Active Site Residues on AChE Reactivation: A QM/MM Approach

    Thomas Driant, Dr. Florian Nachon, Prof. Cyril Ollivier, Prof. Pierre-Yves Renard and Dr. Etienne Derat

    Version of Record online: 23 FEB 2017 | DOI: 10.1002/cbic.201600646

    Thumbnail image of graphical abstract

    Proton transfer during reactivation of organophosphate-inhibited acetylcholinesterase was investigated by QM/MM calculations. These revealed an interplay between protonation states of the catalytic triad and a glutamate close to the active site; these need to be protonated for reactivation to occur.

  2. Molecular Cross-Talk between Nonribosomal Peptide Synthetase Carrier Proteins and Unstructured Linker Regions

    Bradley J. Harden and Prof. Dr. Dominique P. Frueh

    Version of Record online: 22 FEB 2017 | DOI: 10.1002/cbic.201700030

    Thumbnail image of graphical abstract

    Linkers that matter: The solution structure of a peptidyl carrier protein, flanked by its unstructured linkers, revealed contacts between the core and linkers. The interaction was shown to both stabilize the protein core and modulate dynamics in a manner suggestive of allosteric communication.

  3. Alkyl Formate Ester Synthesis by a Fungal Baeyer–Villiger Monooxygenase

    Dr. Felix Martin Ferroni, Carmien Tolmie, Prof. Martha Sophia Smit and Dr. Diederik Johannes Opperman

    Version of Record online: 22 FEB 2017 | DOI: 10.1002/cbic.201600684

    Thumbnail image of graphical abstract

    Choosing sides: Alkyl formate synthesis is performed with a Baeyer–Villiger monooxygenase. Regioselectivity and rate of autohydrolysis of the alkyl formates to the corresponding primary alcohol product is affected by substrate concentration.

  4. Structural Elucidation of a Nonpeptidic Inhibitor Specific for the Human Immunoproteasome

    Dr. Haissi Cui, Regina Baur, Dr. Camille Le Chapelain, Dr. Christian Dubiella, Dr. Wolfgang Heinemeyer, Dr. Eva M. Huber and Prof. Dr. Michael Groll

    Version of Record online: 22 FEB 2017 | DOI: 10.1002/cbic.201700021

    Thumbnail image of graphical abstract

    Better off-site: The binding mechanism of an industrially patented, non-covalent, nonpeptidic, subunit-specific immunoproteasome inhibitor was studied. This revealed distinct interactions that account for its isotype- and species-selectivity towards the human immunoproteasome.

  5. Controlling Cellular Uptake and Toxicity of Polyphenylene Dendrimers by Chemical Functionalization

    Brenton Hammer, Yuzhou Wu, Stephan Fischer, Weina Liu, Tanja Weil and Klaus Müllen

    Accepted manuscript online: 21 FEB 2017 10:20PM EST | DOI: 10.1002/cbic.201700079