ChemBioChem

Cover image for Vol. 16 Issue 11

Editor: Peter Gölitz; Editorial Board Chairs: Thomas Carell, Donald Hilvert, Barbara Imperiali

Online ISSN: 1439-7633

Associated Title(s): ChemCatChem, ChemMedChem, ChemPhysChem, ChemSusChem

Recently Published Issues

See all

Latest News

Browse more news

July 03, 2015

Very Important Paper: Expanding the Imine Reductase Toolbox by Exploring the Bacterial Protein-Sequence Space

Dennis Wetzl, Marco Berrera, Nicolas Sandon, Dan Fishlock, Martin Ebeling, Michael Müller, Steven Hanlon, Beat Wirz, Hans Iding*

Recent investigations on imine reductases (IREDs) have enriched the toolbox of potential catalysts accessing chiral amines. Now, H. Iding and co-workers (F. Hoffmann-La Roche, Switzerland) describe the characterization of 20 new IREDs, which were identified by using a C-terminal domain clustering of the bacterial protein-sequence space. Each of the enzymes was characterized against a set of nine cyclic imine model substrates. A refined clustering towards putative active-site residues was performed that was consistent with the current screen and previously reported results. Finally, preparative (100 mg scale) experiments with two purified IREDs, IR_20 from Streptomyces tsukubaensis and IR_23 from Streptomyces vidiochromogenes, were carried out.

Your Comment...

[Browse more news]

Recently Published Articles

  1. Involvement of Acidic Amino Acid Residues in Zn2+ Binding to Respiratory Complex I

    Dr. Sébastien Kriegel, Dr. Batoul Srour, Dr. Stefan Steimle, Prof. Thorsten Friedrich and Prof. Petra Hellwig

    Article first published online: 31 JUL 2015 | DOI: 10.1002/cbic.201500273

    Thumbnail image of graphical abstract

    Electrochemically induced FTIR difference spectra of the Zn2+-inhibited NADH:ubiquinone oxidoreductase from E. coli reveal conformational changes and shifts of signals at very high wavenumbers (1781 and 1756 cm−1). They point towards the perturbation of acidic residues in a highly hydrophobic environment, including D563 in the membrane arm.

  2. PNA Molecular Beacons Assembled by Post-Synthetic "Click" Chemistry Functionalization

    Xiaoxiao Wang and Robert H.E. Hudson

    Accepted manuscript online: 29 JUL 2015 09:32AM EST | DOI: 10.1002/cbic.201500248

  3. Capturing the direct binding of CFTR correctors to CFTR using click chemistry

    Chandrima Sinha, Weiqiang Zhang, Chang Suk Moon, Marcelo Actis, Sunitha Yarlagadda, Kavisha Arora, Koryse Woodroofe, John P. Clancy, Songbai Lin, Assem G. Ziady, Raymond Frizzell, Naoaki Fujii and Anjaparavanda P. Naren

    Accepted manuscript online: 29 JUL 2015 09:31AM EST | DOI: 10.1002/cbic.201500123

  4. Expanding the Genetic Code for a Dinitrophenyl Hapten

    Wei Ren, Dr. Ao Ji, Michael X. Wang and Prof. Dr. Hui-wang Ai

    Article first published online: 28 JUL 2015 | DOI: 10.1002/cbic.201500204

    Thumbnail image of graphical abstract

    Something unnatural about it: A dinitrophenyl (DNP)-containing unnatural amino acid was genetically encoded for the preparation of hapten-labeled proteins. This small hapten moiety was able to induce selective interactions with anti-DNP antibodies. The capability of genetically introducing DNP into proteins has potential for applications in biosensing and bioseparation, immunology, and therapeutics.

SEARCH

SEARCH BY CITATION