Cover image for Vol. 18 Issue 6

Editor: Meghan Campbell; Editorial Board Chairs: Thomas Carell, Donald Hilvert, Barbara Imperiali

Online ISSN: 1439-7633

Associated Title(s): ChemCatChem, ChemMedChem, ChemPhysChem, ChemSusChem

Recently Published Issues

See all

Latest News

Browse more news

March 14, 2017

Very Important Paper: Identifying Unknown Enzyme-Substrate Pairs from the Cellular Milieu with Native Mass Spectrometry

Kalli C. Catcott, Jing Yan, Wanlu Qu, Vicki H. Wysocki*, Zhaohui Sunny Zhou*

The enzyme-substrate complex is inherently transient, rendering its detection difficult. Therefore, V. Wysocki (The Ohio State University, U.S.A.), Z.S. Zhou (Northeastern University, USA) and co-workers developed a framework, IsoLAIT (Isotope-Labeled, Activity-based Identification and Tracking), for bisubstrate systems. Using this method a common substrate, such as S-adenosylmethionine for methyltransferases, is replaced by an analogue (e.g., S-adenosylvinthionine) that, as a probe, creates a tightly bound [enzyme•substrate-probe] complex upon catalysis by thiopurine-S-methyltransferase. Then, this persistent complex is identified by native mass spectrometry from the cellular milieu without separation. Furthermore, the probe’s isotope pattern flags even unknown substrates and enzymes. IsoLAIT can be broadly applicable for other enzyme systems, particularly those catalyzing group transfer and with multiple substrates.

Your Comment...

[Browse more news]

Recently Published Articles

  1. Metabolic Chemical Reporters of Glycans Exhibit Cell-Type-Selective Metabolism and Glycoprotein Labeling

    Anna R. Batt, Balyn W. Zaro, Marisol X. Navarro and Prof. Dr. Matthew R. Pratt

    Version of Record online: 28 MAR 2017 | DOI: 10.1002/cbic.201700020

    Thumbnail image of graphical abstract

    Metabolism matters: Metabolic chemical reporters (MCRs) of protein glycosylation are monosaccharide analogues bearing bioorthogonal functional groups that can be used for the installation of tags. Here, we show that cell lines have different capacities for the metabolism of MCRs and that classes of glycosylation are differentially affected by the buildup of the corresponding donor sugars.

  2. Controlling Cellular Uptake and Toxicity of Polyphenylene Dendrimers by Chemical Functionalization

    Dr. Brenton A. G. Hammer, Dr. Yuzhou Wu, Stephan Fischer, Weina Liu, Prof. Tanja Weil and Prof. Klaus Müllen

    Version of Record online: 28 MAR 2017 | DOI: 10.1002/cbic.201700079

    Thumbnail image of graphical abstract

    The monodisperse and shape-persistent nature of polyphenylene dendrimers (PPDs) make them a unique class of macromolecules. We investigated whether it is the specific chemical functionalities, relative quantities of each moiety, or the “patched” surface patterning on the dendrimers that more significantly influences their behavior in biological media.

  3. Small molecules targeting human N-acetylmannosamine kinase

    Stephan Hinderlich, Martin Neuenschwander, Paul-Robin Wratil, Andreas Oder, Michael Lisurek, Long D. Nguyen, Jens Peter von Kries and Christian Hackenberger

    Accepted manuscript online: 27 MAR 2017 12:20PM EST | DOI: 10.1002/cbic.201700066

  4. Mapping the interactions of selective biochemical probes of antibody conformation by hydrogen-deuterium exchange mass spectrometry

    Ulrike Leurs, Hermann Beck, Lea Bonnington, Ingo Lindner, Ewa Pol and Kasper Dyrberg Rand

    Accepted manuscript online: 27 MAR 2017 09:21AM EST | DOI: 10.1002/cbic.201600670

  5. Small molecules deep from the gut

    Michael Ehrmann and Markus Kaiser

    Accepted manuscript online: 27 MAR 2017 04:31AM EST | DOI: 10.1002/cbic.201700165