ChemBioChem

Cover image for Vol. 17 Issue 3

Editor: Meghan Campbell; Editorial Board Chairs: Thomas Carell, Donald Hilvert, Barbara Imperiali

Online ISSN: 1439-7633

Associated Title(s): ChemCatChem, ChemMedChem, ChemPhysChem, ChemSusChem

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January 27, 2016

Very Important Paper: Enhancing DNA Crystal Durability through Chemical Crosslinking

Diana Zhang & Paul J. Paukstelis*

Three-dimensional DNA crystals are promising materials for nanotechnology applications. However, these applications may be limited by features inherent to the DNA lattice, including low thermal stability, high cation requirements, and nuclease sensitivity. To overcome these problems D. Zhang & P.J. Paukstelis (University of Maryland) have developed a post-crystallization crosslinking step that enhances the durability of a model DNA crystal lattice. Crystals crosslinked with nornitrogen mustard show enhanced thermal stability, decreased divalent cation requirements, and almost complete resistance to DNase I. This method for stabilizing DNA crystals may be applicable to other DNA constructs and ultimately improve the usefulness of DNA nanoarchitectures.

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Recently Published Articles

  1. Characterization of the Shigella and Salmonella Type III Secretion System Tip–Translocon Protein–Protein Interaction by Paramagnetic Relaxation Enhancement

    Kawaljit Kaur, Dr. Srirupa Chatterjee and Dr. Roberto N. De Guzman

    Article first published online: 10 FEB 2016 | DOI: 10.1002/cbic.201500556

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    PREview of coming attractions: We used paramagnetic relaxation enhancement to confirm a direct protein–protein interaction between the major translocon proteins of Shigella and Salmonella and their cognate tip proteins. Our findings provide new insights into the interactions involved in the assembly of the type III secretion system needle apparatus.

  2. Influence of Pre-organised Architecture on Cell Adhesion by Using Multivalent RGD Compounds

    Dr. Adrien Grassin, Dr. Muriel Jourdan, Prof. Pascal Dumy and Dr. Didier Boturyn

    Article first published online: 10 FEB 2016 | DOI: 10.1002/cbic.201500495

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    Grabbing with all four hands: Tetrameric RGD-containing macromolecules were designed by varying the interligand distances. We show, by both biochemical investigation and molecular modelling, the importance of well pre-organised architecture of multivalent RGD compounds for the binding avidity of αVβ3 receptor.

  3. Crystallization of Enantiomerically Pure Proteins from Quasi-Racemic Mixtures: Structure Determination by X-Ray Diffraction of Isotope-Labeled Ester Insulin and Human Insulin

    Dr. Kalyaneswar Mandal, Dr. Balamurugan Dhayalan, Dr. Michal Avital-Shmilovici, Prof. Dr. Andrei Tokmakoff and Prof. Dr. Stephen B. H. Kent

    Article first published online: 10 FEB 2016 | DOI: 10.1002/cbic.201500600

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    Getting the l out of there: The chemically synthesized proteins [(1-13C=18O)PheB24)] ester insulin and [(1-13C=18O)PheB24)] human insulin spontaneously crystallized as l-proteins from quasi-racemic mixtures with a closely related d-protein, DKP insulin, and were used to determine the crystal structures of the isotope-labeled ester insulin and human insulin protein molecules.

  4. YedY: A Mononuclear Molybdenum Enzyme with a Redox-Active Ligand?

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    Article first published online: 10 FEB 2016 | DOI: 10.1002/cbic.201600004

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    Article first published online: 10 FEB 2016 | DOI: 10.1002/cbic.201500656

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    Unwinding underway: We synthesized and purified peptides to evaluate factors by solid-state NMR that could stabilize a tilted orientation of transmembrane helices. Helix end fraying was identified as a critical feature for stabilizing the orientations and limiting the dynamics of transmembrane helices in membrane proteins.

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