Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
January 15, 2015
Very Important Paper: Synthesis of 3'-Fluoro-tRNA Analogues for Exploring Non-ribosomal Peptide Synthesis in Bacteria
Laura Iannazzo, Guillaume Laisné, Matthieu Fonvielle, Emmanuelle Braud, Jean-Philippe Herbeuval, Michel Arthur, Mélanie Etheve-Quelquejeu*
Among a wide range of metabolic pathways, aminoacyl-tRNAs participate in the synthesis of the peptidoglycan network in the cell wall of bacterial pathogens. To understand the mechanism of these reactions, Mélanie Etheve-Quelquejeu (Université Paris Descartes) and co-workers report on a semi-synthetic route to 3' fluorinated dinucleotides analogues of Ala-tRNAAla with the xylo and ribo configurations and the acylated form of the xylo isomer. The conformation of the 3' fluorinated sugar was also analyzed and it was found that the xylo stereoisomer preferentially adopts a North conformation, while ribo stereoisomers prefer the South conformation.
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