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September 21, 2015
Very Important Paper: Characterization of Early Enzymes Involved in TDP-Aminodideoxypentose Biosynthesis en Route to Indolocarbazole AT2433
Pauline Peltier-Pain, Shanteri Singh, Jon S. Thorson*
While a number of bacterial natural products contain both uniquely functionalized five- and six-carbon sugars, there has not been extensive investigation into the biosynthesis of five-carbon sugars. Now, J. S. Thorson (University of Kentucky, USA) and co-workers report the study of key enzymes involved in the early steps of five-carbon sugar modification in which unique real-time nuclear magnetic resonance methods serve as a primary basis for biochemical analysis. Their studies reveal, for the first time, evidence to suggest that the same enzymes may be employed in modifying both five-carbon and six-carbon sugars, raising the question of whether enzyme conservation is a common phenomenon or unique to the selected model.
Recently Published Articles
- Effects of the Surface Densities of Glycoclusters on the Determination of Their IC50 and Kd Value Determination by Using a Microarray
Lucie Dupin, Francesca Zuttion, Dr. Thomas Géhin, Albert Meyer, Prof. Dr. Magali Phaner-Goutorbe, Dr. Jean-Jacques Vasseur, Dr. Eliane Souteyrand, Dr. François Morvan and Dr. Yann Chevolot
Article first published online: 6 OCT 2015 | DOI: 10.1002/cbic.201500371
Making sense of dense: The glycocluster surface densities on microarrays were modulated to investigate the impact on measuring IC50 and Kd values. The results show that measured IC50 values increase proportionally with glycocluster surface density. In contrast, measured Kd values are not influenced by glycocluster surface density.
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Jutta Siegrist, Simon Aschwanden, Silja Mordhorst, Linda Thöny-Meyer, Michael Richter and Jennifer Nina Andexer
Accepted manuscript online: 6 OCT 2015 05:18AM EST | DOI: 10.1002/cbic.201500410
- Streptocollin, a type IV lanthipeptide produced by Streptomyces collinus Tü 365
Dumitrita Iftime, Martin Jasyk, Andreas Kulik, Johannes F. Imhoff, Evi Stegmann, Wolfgang Wohlleben, Roderich D. Süssmuth and Tilmann Weber
Accepted manuscript online: 6 OCT 2015 05:16AM EST | DOI: 10.1002/cbic.201500377
- Involvement of Lipocalin-like CghA in Decalin-Forming Stereoselective Intramolecular [4+2] Cycloaddition
Dr. Michio Sato, Dr. Fumitoshi Yagishita, Prof. Dr. Takashi Mino, Dr. Nahoko Uchiyama, Dr. Ashay Patel, Dr. Yit-Heng Chooi, Dr. Yukihiro Goda, Dr. Wei Xu, Prof. Dr. Hiroshi Noguchi, Tsuyoshi Yamamoto, Prof. Dr. Kinya Hotta, Prof. Dr. Kendall N. Houk, Prof. Dr. Yi Tang and Prof. Dr. Kenji Watanabe
Article first published online: 2 OCT 2015 | DOI: 10.1002/cbic.201500386
Decalin decadence: The C. globosum gene cluster responsible for the biosynthesis of Sch 210972, a potential anti-HIV fungal polyketide–nonribosomal peptide hybrid natural product, has been identified. Genetic, biochemical and computational studies revealed the function of four enzymes involved. Most notably, lipocalin-like CghA facilitates a stereoselective intramolecular [4+2] cycloaddition to form the decalin core.
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Heiko Fittler, Alexander Depp, Olga Avrutina, Sven O. Dahms, Manuel E. Than, Martin Empting and Harald Kolmar
Accepted manuscript online: 1 OCT 2015 04:00PM EST | DOI: 10.1002/cbic.201500447