Cover image for Vol. 15 Issue 16

Editor: Peter Gölitz; Editorial Board Chairs: Thomas Carell, Donald Hilvert, Barbara Imperiali

Online ISSN: 1439-7633

Associated Title(s): ChemCatChem, ChemMedChem, ChemPhysChem, ChemSusChem

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November 17, 2014

Very Important Paper: Unambiguous Assignment of Short- and Long-Range Structural Restraints by Solid-State NMR using Segmental Isotope Labeling

Tobias Schubeis, Torsten Lührs, Christiane Ritter*

Solid-state NMR has become a powerful technique for the elucidation of the structures of large protein complexes. However, spectral complexity and sensitivity remain serious challenges. Protein trans-splicing by the split intein DnaE from Nostoc punctiforme allows specific segments of a full-length protein to be isotopically labeled under a wide variety of conditions. Christiane Ritter (Helmholtz Centre for Infection Research, Germany) and co-workers have now employed this approach to obtain solid-state NMR spectra of a fungal prion protein (HET-s) with significantly improved spectral quality. Protein trans-splicing can be easily combined with other isotope labeling strategies to facilitate the resonance assignment and collection of structural restraints of proteins by solid-state NMR.

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Recently Published Articles

  1. Cotranscriptionally Folded RNA Nanostructures Pave the Way to Intracellular Nanofabrication

    Dr. Jiang Li, Dr. Jie Chao, Jiye Shi and Prof. Dr. Chunhai Fan

    Article first published online: 21 NOV 2014 | DOI: 10.1002/cbic.201402627

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    Go out on the tiles: Well-defined RNA architectures are readily assembled by cotranscriptional folding of single-stranded RNA tiles in an isothermal environment; this represents an important step toward intracellular fabrication of nanodevices for theranostic applications.

  2. Active Site Targeting of Hedgehog Precursor Protein with Phenylarsine Oxide

    Timothy S. Owen, Xie Jian Xie, Benjamin Laraway, Dr. George Ngoje, Prof. Chunyu Wang and Prof. Brian P. Callahan

    Article first published online: 21 NOV 2014 | DOI: 10.1002/cbic.201402421

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  3. SUMO-Mimicking Peptides Inhibiting Protein SUMOylation

    Dr. Bo Zhao, Eric B. Villhauer, Karan Bhuripanyo, Prof. Dr. Hiroaki Kiyokawa, Prof. Dr. Hermann Schindelin and Prof. Dr. Jun Yin

    Article first published online: 20 NOV 2014 | DOI: 10.1002/cbic.201402472

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    Article first published online: 18 NOV 2014 | DOI: 10.1002/cbic.201402547

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