Cover image for Vol. 18 Issue 6

Editor: Meghan Campbell; Editorial Board Chairs: Thomas Carell, Donald Hilvert, Barbara Imperiali

Online ISSN: 1439-7633

Associated Title(s): ChemCatChem, ChemMedChem, ChemPhysChem, ChemSusChem

Recently Published Issues

See all

Latest News

Browse more news

March 14, 2017

Very Important Paper: Identifying Unknown Enzyme-Substrate Pairs from the Cellular Milieu with Native Mass Spectrometry

Kalli C. Catcott, Jing Yan, Wanlu Qu, Vicki H. Wysocki*, Zhaohui Sunny Zhou*

The enzyme-substrate complex is inherently transient, rendering its detection difficult. Therefore, V. Wysocki (The Ohio State University, U.S.A.), Z.S. Zhou (Northeastern University, USA) and co-workers developed a framework, IsoLAIT (Isotope-Labeled, Activity-based Identification and Tracking), for bisubstrate systems. Using this method a common substrate, such as S-adenosylmethionine for methyltransferases, is replaced by an analogue (e.g., S-adenosylvinthionine) that, as a probe, creates a tightly bound [enzyme•substrate-probe] complex upon catalysis by thiopurine-S-methyltransferase. Then, this persistent complex is identified by native mass spectrometry from the cellular milieu without separation. Furthermore, the probe’s isotope pattern flags even unknown substrates and enzymes. IsoLAIT can be broadly applicable for other enzyme systems, particularly those catalyzing group transfer and with multiple substrates.

Your Comment...

[Browse more news]

Recently Published Articles

  1. "Head-to-head" Prenyl Synthases in Some Pathogenic Bacteria

    Christopher J. Schwalen, Xinxin Feng, Weidong Liu, Bing O-Dowd, Tzu-Ping Ko, Christopher J. Shin, Rey-Ting Guo, Douglas A. Mitchell and Eric Oldfield

    Accepted manuscript online: 24 MAR 2017 12:02PM EST | DOI: 10.1002/cbic.201700099

  2. Hexaphyrin as a Potential Theranostic Dye for Photothermal Therapy and 19F Magnetic Resonance Imaging

    Dr. Tomohiro Higashino, Hirotaka Nakatsuji, Ryosuke Fukuda, Haruki Okamoto, Dr. Hirohiko Imai, Prof. Dr. Tetsuya Matsuda, Prof. Dr. Hidehito Tochio, Prof. Dr. Masahiro Shirakawa, Prof. Dr. Nikolai V. Tkachenko, Prof. Dr. Mitsuru Hashida, Prof. Dr. Tatsuya Murakami and Prof. Dr. Hiroshi Imahori

    Version of Record online: 24 MAR 2017 | DOI: 10.1002/cbic.201700071

    Thumbnail image of graphical abstract

    meso-Aryl-substituted expanded porphyrins have two key features as potential theranostic agents: excellent NIR absorption and the possibility for the introduction of multiple fluorine atoms at the meso-aryl groups. Hexaphyrin exhibited an intense photothermal effect and potential for use in visualization by 19F MRI. These results shed light on the utility of expanded porphyrins as theranostic agents.

  3. Discovering Drugs with DNA-Encoded Library Technology: From Concept to Clinic with an Inhibitor of Soluble Epoxide Hydrolase

    Dr. Svetlana L. Belyanskaya, Dr. Yun Ding, Dr. James F. Callahan, Dr. Aili L. Lazaar and Dr. David I. Israel

    Version of Record online: 24 MAR 2017 | DOI: 10.1002/cbic.201700014

    Thumbnail image of graphical abstract

    We describe the development of a DNA-encoded small-molecule technology platform, and its use in discovering the soluble epoxide hydrolase (sEH) inhibitor GSK2256294. This molecule progressed through preclinical development and entered a human clinical trial where it was found to be orally bioavailable and well tolerated, demonstrating potent and dose-dependent inhibition of sEH.

  4. Amine transaminase engineering for spatially bulky substrate acceptance

    Uwe Bornscheuer, Martin S. Weiß, Ioannis V. Pavlidis, Paul Spur, Steven P. Hanlon, Beat Wirz and Hans Iding

    Accepted manuscript online: 23 MAR 2017 10:00AM EST | DOI: 10.1002/cbic.201700033

  5. Conjugate Vaccines from Bacterial Antigens by Squaric Acid Chemistry: A Closer Look

    Dr. Peng Xu, Meagan Kelly, Dr. Willie F. Vann, Dr. Firdausi Qadri, Dr. Edward T. Ryan and Dr. Pavol Kováč

    Version of Record online: 23 MAR 2017 | DOI: 10.1002/cbic.201600699

    Thumbnail image of graphical abstract

    Fair and square: A new protocol for conjugating delipidated bacterial polysaccharides to proteins by using squarate chemistry shows a twofold increase in conjugation efficiency. The spent reagent can be regenerated, and the conjugate vaccine made from the conjugation reagent is comparable with the vaccine made from the parent antigen. CP: carrier protein; OSPc: O-SP–core.