Metabolic Glycan Imaging by Isonitrile–Tetrazine Click Chemistry
Shaun Stairs, Dr. André A. Neves, Dr. Henning Stöckmann, Yelena A. Wainman, Dr. Heather Ireland-Zecchini, Prof. Kevin M. Brindle and Dr. Finian J. Leeper
Article first published online: 13 MAY 2013 | DOI: 10.1002/cbic.201300130
Seeing the sugar coating: N-Acetyl-glucosamine and mannosamine derivatives tagged with an isonitrile group are metabolically incorporated into cell-surface glycans and can be detected with a fluorescent tetrazine. This bioorthogonal isonitrile–tetrazine ligation is also orthogonal to the commonly used azide-cyclooctyne ligation, and so will allow simultaneous detection of the incorporation of two different sugars.