ChemBioChem

Cover image for Vol. 18 Issue 10

Editor: Meghan Campbell; Editorial Board Chairs: Thomas Carell, Donald Hilvert, Barbara Imperiali

Online ISSN: 1439-7633

Associated Title(s): ChemCatChem, ChemMedChem, ChemPhysChem, ChemSusChem

Cover Picture: A New Chemical Probe for Proteomics of Carbohydrate-Binding Proteins (ChemBioChem 2/2005)

6_02/2005The cover picture shows how galectins are selected from a mixture of proteins by a novel probe. The probe noncovalently binds the medicinally relevant galectins, and its photoaffinity label covalently captures the protein. The captured protein is visualized by a fluorescent dye that is introduced by chemoselective 1,2,3-triazole formation or "click" chemistry. The methodology represents a step towards comparative quantification of the galectin amounts in proteomes, which is relevant, for example, for prognosis with respect to the malignancy of tissues. Despite the low affinities in protein–carbohydrate interactions, the probe shown is able to select the galectins from a mixture of four proteins. For more information, see the article by R. J. Pieters et al on p. 291 ff.

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