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Cover Picture: Fluorescence-Based Cloning of a Protein Tyrosine Kinase with a Yeast Tribrid System (ChemBioChem 8/2005)
The cover picture shows a new approach for the functional cloning of enzymes involved in the post-translational modification of proteins. This strategy employs a yeast tribrid system: a variant of the yeast two-hybrid system that couples activation of a reporter gene to expression of an enzyme. The image on the right is a combined differential interference contrast / confocal-laser scanning micrograph of S. cerevisiae transiently expressing the human Fyn protein tyrosine kinase. Cells expressing this enzyme trigger a reporter gene that encodes enhanced green fluorescent protein; this enables isolation of hits by fluorescence-activated cell sorting. This system is discussed in more detail on p. 1442 ff by B. R. Peterson and D. D. Clark.