Improved Sensitivity for Imaging Spin Trapped Hydroxyl Radical at 250 MHz
Dr. Joshua R. Biller, Dr. Mark Tseitlin, Dr. Deborah G. Mitchell, Zhelin Yu, Laura A. Buchanan, Dr. Hanan Elajaili, Dr. Gerald M. Rosen, Dr. Joseph P. Y. Kao, Dr. Sandra S. Eaton and Gareth R. Eaton
Article first published online: 8 DEC 2014 | DOI: 10.1002/cphc.201402835
Three′s a charm: Reactive oxygen species can be detected by spin trapping. Previously high detection limits at the low frequencies required for in vivo EPR imaging are dramatically reduced by combining three enabling technologies: the spin-trap BMPO, rapid-scan EPR and a new image reconstruction algorithm, and demonstrated by a 2D spectral spatial image of 5 μM BMPO–OH.