© WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Editor: Greta Heydenrych; Editorial Board Chairs: Christian Amatore, Michael Grätzel, Michel Orrit
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ISI Journal Citation Reports © Ranking: 2015: 8/35 (Physics Atomic Molecular & Chemical); 50/144 (Chemistry Physical)
Online ISSN: 1439-7641
The cover picture shows two images of the same surface (6.3×6.3 μm2), in which single molecules of the green fluorescent protein mutant T203V/E222Q are immobilized in a polyacrylamide gel. Illumination with a single color (476 nm at 1.5 kW cm−2) excites the molecules to fluoresce weakly, as measured by counts per unit time from a localized area. Simultaneous two-color illumination (476 nm at 1.5 kW cm−2 as before plus 407 nm at 0.3 kW cm−2) leads to a dramatic increase in signal intensity. The time lag between the recording of two images is several minutes—too long to exclude, for example, slow rotational dynamics of the embedded molecules—and thereby prevents determination of exact values for the fluorescence signal increase of the individual fluorophores. Find out more in the communication by Zumbusch et al. on pages 392–396.