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Editor: Greta Heydenrych; Editorial Board Chairs: Christian Amatore, Michael Grätzel, Michel Orrit
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Online ISSN: 1439-7641
October 07, 2011
VIP: Optical Modulation and Selective Recovery of Cy5 Fluorescence
Chaoyang Fan, Jung-Cheng Hsiang, and Robert M. Dickson*
A new sensitivity-boosting approach that could be applied to a wide range of fluorophores is presented. The method, introduced by R. M. Dickson and co-workers, has great potential in applications ranging from super-resolution microscopy to in vivo animal imaging. It is known that fluorescence detection is often limited by competing background emission. In a new twist, the authors co-illuminate highly emissive Cy5 with one laser to excite fluorescence, and a second one (at much lower energy) to rapidly depopulate photoinduced dark states. Intensity modulating this secondary illumination does not increase background, but dynamically increases fluorescence. This Cy5-specific fluorescence modulation shifts Cy5 emission to a specific detection frequency, thereby avoiding background fluorescence and drastically improving sensitivity.
Received August 31, 2011, published online November 15, 2011, DOI: 10.1002/cphc.201100671.