Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
February 20, 2014
VIP: Two-Color RESOLFT Nanoscopy with Green and Red Fluorescent Photochromic Proteins
Flavie Lavoie-Cardinal, Nickels A. Jensen, Volker Westphal, Andre C. Stiel, Andriy Chmyrov, Jakob Bierwagen, Ilaria Testa, Stefan Jakobs,* Stefan W. Hell*
Dual-color live-cell RESOLFT superresolution microscopy is demonstrated with green- and red-emitting switchable fluorescent protein tags. The red-emitting tag is a novel reversibly switchable protein, rsCherryRev1.4, enabling a resolution up to four times over the diffraction barrier. Dual-color RESOLFT works with both single and multiple ‘donuts’ using more than 23000 ‘donuts’ in parallel.
Received November 04, 2013, published online January 21, 2014, DOI: 10.1002/cphc.201301016 – read now.