ChemPhysChem

Cover image for Vol. 15 Issue 13

Editor: Greta Heydenrych; Editorial Board Chairs: Christian Amatore, Michael Grätzel, Michel Orrit

Online ISSN: 1439-7641

Associated Title(s): Advanced Materials, ChemBioChem, ChemCatChem, ChemElectroChem, ChemSusChem, Small

February 20, 2014

VIP: Two-Color RESOLFT Nanoscopy with Green and Red Fluorescent Photochromic Proteins

Flavie Lavoie-Cardinal, Nickels A. Jensen, Volker Westphal, Andre C. Stiel, Andriy Chmyrov, Jakob Bierwagen, Ilaria Testa, Stefan Jakobs,* Stefan W. Hell*

Dual-color live-cell RESOLFT superresolution microscopy is demonstrated with green- and red-emitting switchable fluorescent protein tags. The red-emitting tag is a novel reversibly switchable protein, rsCherryRev1.4, enabling a resolution up to four times over the diffraction barrier. Dual-color RESOLFT works with both single and multiple ‘donuts’ using more than 23000 ‘donuts’ in parallel.

Received November 04, 2013, published online January 21, 2014, DOI: 10.1002/cphc.201301016 – read now.

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