Biotechnology and Applied Biochemistry

Cover image for Vol. 63 Issue 3

Edited By: G. Gilardi and Jian-Jiang Zhong

Impact Factor: 1.429

ISI Journal Citation Reports © Ranking: 2015: 118/161 (Biotechnology & Applied Microbiology); 240/289 (Biochemistry & Molecular Biology)

Online ISSN: 1470-8744

Associated Title(s): Biochemistry and Molecular Biology Education, BioFactors, IUBMB Life

Featured

  • In silico analysis of human Toll-like receptor 7 ligand binding domain

    In silico analysis of human Toll‐like receptor 7 ligand binding domain

    Determination of molecular interactions between hTLR7 ECD homodimer model (Chain A in magenta and Chain B in cyan) and viral ssRNA (green). The interacting residues are presented by CPK.

  • Molecular cloning and transgenic characterization of the genes encoding chalcone synthase and chalcone isomerase from the Tibetan herbal plant Mirabilis himalaica

    Molecular cloning and transgenic characterization of the genes encoding chalcone synthase and chalcone isomerase from the Tibetan herbal plant Mirabilis himalaica

    Plants of Mirabilis himalaica growing in the wild.

  • Expression of anti-tumor necrosis factor alpha (TNFα) single-chain variable fragment (scFv) in Spirodela punctata plants transformed with Agrobacterium tumefaciens

    Expression of anti‐tumor necrosis factor alpha (TNFα) single‐chain variable fragment (scFv) in Spirodela punctata plants transformed with Agrobacterium tumefaciens

    Histochemical assay for GUS expression in S. punctata, observed as dark blue spots in transformed S. punctata (a, b, c) as compared with that of untransformed plant (d).

  • Carboxymethyl starch/alginate microspheres containing diamine oxidase for intestinal targeting

    Carboxymethyl starch/alginate microspheres containing diamine oxidase for intestinal targeting

    Macroscopic image of CMS/alginate microbeads after Coomassie blue staining: (A) without enzyme and (B) with entrapped enzyme.

  • Aluminum oxide nanoparticles alter cell cycle progression through CCND1 and EGR1 gene expression in human mesenchymal stem cells

    Aluminum oxide nanoparticles alter cell cycle progression through CCND1 and EGR1 gene expression in human mesenchymal stem cells

    Morphological changes in hMSC treated with Al2O3-NPs for 48 H (×400). Microscopic examination of hMSC treated with Al2O3-NPs: (a and d) untreated control cells; (b and e) cells treated with dose-1 of Al2O3-NPs; and (c and f) cells treated with dose-2 of Al2O3-NPs. The bright field images (a–c) in the left column are shown for morphological comparison. Propidium iodide (PI) staining shows (d–f) apoptotic cells with chromosomal condensation and nuclear fragmentation, and viable cells with intact nuclei. Abnormal cell morphology is shown by yellow arrow points (scale bar: 100 µm). The experiments were repeated at least in triplicate with similar results as shown.

  • Overexpression and purification of HSV-2 glycoprotein D in suspension CHO cells with serum-free medium and immunogenicity analysis

    Overexpression and purification of HSV‐2 glycoprotein D in suspension CHO cells with serum‐free medium and immunogenicity analysis

    Dot blot or SDS-PAGE analysis of surviving cell clones amplified with different concentrations of MTX. (A) Dot blot analysis of 90 cell clones survived after incubation in selection medium with 0 nmol/L of MTX; (B) Dot blot analysis of 28 cell clones survived after incubation in selection medium with 50 nmol/L of MTX; (C) Dot blot analysis of 18 cell clones survived after incubation in selection medium with 200 nmol/L of MTX; (D) SDS-PAGE analysis of 10 cell clones survived after incubation in selection medium with 500 nmol/L of MTX. Lane or dot NC, the culture supernatant of CHO-DG44 cells transfected with pMD902. Dot P, mouse sera as positive control. Lane M, standard molecular marker. The number of each cell clone was indicated above the dot or lane. The arrow in 2D indicated the size of the interest recombinant protein.

  • Isolation and characterization of a new [FeFe]-hydrogenase from Clostridium perfringens

    Isolation and characterization of a new [FeFe]‐hydrogenase from Clostridium perfringens

    CpHydA cloning, purification, and model structure. (A) Map of the expression vector pECPF2655. (B) Coomassie-stained SDS-PAGE of the purified CpHydA; molecular weights are in kDa. (C) Scheme of the modular domains, where yellow cross = [2Fe2S] plant ferredoxin-like; red diamond = [4Fe4S] coordinated by three cysteines and one histidine; green oval  = 2[4Fe4S] bacterial ferredoxin-like; blue rectangle = H-domain. (D) Homology model of CpHydA structure.

  • In silico analysis of human Toll‐like receptor 7 ligand binding domain
  • Molecular cloning and transgenic characterization of the genes encoding chalcone synthase and chalcone isomerase from the Tibetan herbal plant Mirabilis himalaica
  • Expression of anti‐tumor necrosis factor alpha (TNFα) single‐chain variable fragment (scFv) in Spirodela punctata plants transformed with Agrobacterium tumefaciens
  • Carboxymethyl starch/alginate microspheres containing diamine oxidase for intestinal targeting
  • Aluminum oxide nanoparticles alter cell cycle progression through CCND1 and EGR1 gene expression in human mesenchymal stem cells
  • Overexpression and purification of HSV‐2 glycoprotein D in suspension CHO cells with serum‐free medium and immunogenicity analysis
  • Isolation and characterization of a new [FeFe]‐hydrogenase from Clostridium perfringens

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A chimeric protein encompassing hepatitis C virus epitopes is able to elicit both humoral and cell-mediated immune responses in mice
Daylen Aguilar-Noriega, Liz Alvarez-Lajonchere, Emma Brown, Felix Leonardo Santana, Jean Dubuisson, Czeslaw Wychowski, Ivis Guerra1, Gillian Martínez-Donato, Angel Pérez, Yalena Amador-Cañizares and Santiago Dueñas-Carrera
Volume 61, Issue 6

Infection by the hepatitic C virus is a serious problem for human health and ti date there are vaccines available against this pathogen. This work describes a chimeric protein (Eq1) encompassing hepatitic C virus amino acid regions from structural antigens expressed in bacterial. Eq1 was found to be recognized by anti- hepatitic C human sera and to be immunogenic in mice.

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