Biotechnology and Applied Biochemistry

Cover image for Vol. 61 Issue 5

Edited By: G. Gilardi and J.J. Zhong

Impact Factor: 1.322

ISI Journal Citation Reports © Ranking: 2013: 119/165 (Biotechnology & Applied Microbiology); 247/291 (Biochemistry & Molecular Biology)

Online ISSN: 1470-8744

Associated Title(s): Biochemistry and Molecular Biology Education, BioFactors, IUBMB Life

Featured

  • Characterization of the 5′ flanking region of lipase gene from Penicillium expansum and its application in molecular breeding

    Characterization of the 5′ flanking region of lipase gene from Penicillium expansum and its application in molecular breeding

    Schematic illustration of the recombinant vectors pCHAMBIA2302-Ppel::GUS::TtrpC and pCHAMBIA2302-Ppel::PEL::TtrpC. PtrpC, A. nidulans trpC promoter; Hyg B, hygromycin B phosphostransferase gene; TtrpC, A. nidulans trpC terminator; Ppel, the 5′ flanking sequence of PEL; PEL, lipase gene of P. expansumPE-12; GUS, β-glucuronidase gene. Restriction enzyme sites are indicated.

  • Optimization of 2,3-butanediol production by Enterobacter cloacae in simultaneous saccharification and fermentation of corncob residue

    Optimization of 2,3‐butanediol production by Enterobacter cloacae in simultaneous saccharification and fermentation of corncob residue

    The response surface plot and the corresponding contour plot on BA production by E. cloacaeUV-4. Urea, LA, SC, and MgSO4 levels were 1.663, 2.090, 0.454, and 0.309 g/L, respectively.

  • Scaffold/matrix attachment regions from CHO cell chromosome enhanced the stable transfection efficiency and the expression of transgene in CHO cells

    Scaffold/matrix attachment regions from CHO cell chromosome enhanced the stable transfection efficiency and the expression of transgene in CHO cells

    Plasmid vectors used in this study. The integration vectors were based on the pEGFP-C1 backbone, and the enhanced green fluorescent protein (EGFP) was replaced by the targeted protein gene under control of the cytomegalovirus promoter (CMV) immediate early promoter. The vectors carry the GS gene cassettes for selection and amplification. In addition, the S/MARs fragments were inserted in the 5′ region of the CMV promoter. Control plasmid pCMcLys with the chicken lysozyme gene 5′ S/MARs. Expression plasmids pCMmar1 and pCMmar6 containing two different S/MARs replace the chicken lysozyme gene 5′ S/MARs.

  • Design and characterization of a chimeric multiepitope construct containing CfaB, heat-stable toxoid, CssA, CssB, and heat-labile toxin subunit B of enterotoxigenic Escherichia coli: a bioinformatic approach

    Design and characterization of a chimeric multiepitope construct containing CfaB, heat‐stable toxoid, CssA, CssB, and heat‐labile toxin subunit B of enterotoxigenic Escherichia coli: a bioinformatic approach

    Ramachandran plot of the chimeric 3CL protein for the evaluation of structure validity.

  • Efficient soybean regeneration and Agrobacterium-mediated transformation using a whole cotyledonary node as an explant

    Efficient soybean regeneration and Agrobacterium‐mediated transformation using a whole cotyledonary node as an explant

    Whole cotyledonary node regeneration and transformation system of soybean (Zhong Huang 13). (A) Whole cotyledonary node explants with shoot buds were cultured on a shoot induction medium for 12 days. (B) After culturing for 12 days in a shoot elongation medium, two cotyledons were excised from the explants. (C) and (D) Histochemical GUS staining of positive shoots developed from whole cotyledonary node explants; positive shoots were selected on MSB media with 150 mg/L kanamycin for 15 days. (E)–(G) The putative transgenic plant with the gus gene. (H) Histochemical GUS staining of pollen grains of T0 transgenic plant. (I) and (J) Histochemical GUS staining of pod and seeds of T0 transgenic plants.

  • Oriented covalent immobilization of esterase BioH on hydrophilic-modified Fe3O4 nanoparticles

    Oriented covalent immobilization of esterase BioH on hydrophilic‐modified Fe3O4 nanoparticles

    Schematic of esterase BioH on the carrier surface.

  • Study on antimicrobial potential of neem oil nanoemulsion against Pseudomonas aeruginosa infection in Labeo rohita

    Study on antimicrobial potential of neem oil nanoemulsion against Pseudomonas aeruginosa infection in Labeo rohita

    H&E staining in fish gills, objective—400× magnification. (A) Control gills with occurrence of normal cellular components like CC (chloride cells), PC (pillar cells), PL (primary lamellae), SL (secondary lamellae), MC (mucus cells). (B) DMSO-treated gills. (C) Neem oil-treated gills with alterations like LF (lamellar fusion), DS (desquamation), HT (hypertrophy), OD (edema). (D) Neem nanoemulsion-treated gills with alterations LF (lamellar fusion),SL (shortening of lamellae).

  • Characterization of the 5′ flanking region of lipase gene from Penicillium expansum and its application in molecular breeding
  • Optimization of 2,3‐butanediol production by Enterobacter cloacae in simultaneous saccharification and fermentation of corncob residue
  • Scaffold/matrix attachment regions from CHO cell chromosome enhanced the stable transfection efficiency and the expression of transgene in CHO cells
  • Design and characterization of a chimeric multiepitope construct containing CfaB, heat‐stable toxoid, CssA, CssB, and heat‐labile toxin subunit B of enterotoxigenic Escherichia coli: a bioinformatic approach
  • Efficient soybean regeneration and Agrobacterium‐mediated transformation using a whole cotyledonary node as an explant
  • Oriented covalent immobilization of esterase BioH on hydrophilic‐modified Fe3O4 nanoparticles
  • Study on antimicrobial potential of neem oil nanoemulsion against Pseudomonas aeruginosa infection in Labeo rohita

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Characterization of the 5′ flanking region of lipase gene from Penicillium expansum and its application in molecular breeding
Tian Zhang, Ying Peng, Qingsheng Yu, Jieliang Wang and Kexuan Tang.
Volume 61, Issue 5

A major challenge for further promotion of lipase productivity in Penicillium expansum is to find a suitable promoter for this industrial strain. This work characterized the 5' flanking region of P. expansum lipase (Ppel) containing a putative novel promoter sequence by fusing to β-glucuronidase (GUS), which was subsequently introduced into P. expansum. The transformants, which showed blue color quickly after incubation in GUS detection buffer, implied a strong promoter activity of this fragment. Facilitated by this novel promoter, P. expansum PE-12 was genetically modified, and the highest lipase yield reached 2,100 U/mL, which is over twofold that of a previous industrial strain. The engineered strain showed great potential in lipase industry.

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