Biotechnology and Applied Biochemistry

Cover image for Vol. 62 Issue 2

Edited By: G. Gilardi and Jian-Jiang Zhong

Impact Factor: 1.322

ISI Journal Citation Reports © Ranking: 2013: 119/165 (Biotechnology & Applied Microbiology); 247/291 (Biochemistry & Molecular Biology)

Online ISSN: 1470-8744

Associated Title(s): Biochemistry and Molecular Biology Education, BioFactors, IUBMB Life

Featured

  • Overexpression of artemisinic aldehyde Δ11 (13) reductase gene–enhanced artemisinin and its relative metabolite biosynthesis in transgenic Artemisia annua L.

    Overexpression of artemisinic aldehyde Δ11 (13) reductase gene–enhanced artemisinin and its relative metabolite biosynthesis in transgenic Artemisia annua L.

    Establishment of transgenic A. annua. A: Hygromycin-resistant shoots generated from leaves; B: rooted hygromycin-resistant plantlets; C: acclimated transgenic plants of A. annua; D: transgenic plants of A. annua in field.

  • Production, optimization, and partial purification of lipase from Pseudomonas sp. strain BUP6, a novel rumen bacterium characterized from Malabari goat

    Production, optimization, and partial purification of lipase from Pseudomonas sp. strain BUP6, a novel rumen bacterium characterized from Malabari goat

    Screening for lipase activity. (A) The isolate was grown on nutrient agar medium supplemented with (in 100 mL) 1 mL Tween 80, 0.5 g peptone, 0.5 g NaCl, 0.3 g beef extract, 0.01 g CaCl2, and 1 mL olive oil, at 37 °C for 24 H. Production of lipase was indicated by opaque halo around the colonies. Calcium oleate precipitate formed by the hydrolytic activity of lipase is shown at the inset; (B) the cell-free culture (24 H old) supernatant was introduced into the well bored at the center of chromogenic agar plates and incubated at 37 °C for 1 H. Production of lipase was indicated by the change in color from red to yellow around the colonies.

  • High-yield production of apoplast-directed human adenosine deaminase in transgenic tobacco BY-2 cell suspensions

    High‐yield production of apoplast‐directed human adenosine deaminase in transgenic tobacco BY‐2 cell suspensions

    ADA-specific activities measured in BY-2 calli transformed with various cytosolic and apoplast-directing constructs. Each circle represents ADA activity from an independent transformant, and the yellow diamond denotes the median value. The number of calli assayed is shown in brackets after each construct.

  • Immobilization of lipase on porous monodisperse chitosan microspheres

    Immobilization of lipase on porous monodisperse chitosan microspheres

    Scanning electron microscope images: (a) surface of freeze-dried chitosan microsphere and (b) surface of freeze-dried chitosan microsphere with lipase loaded.

  • Suppression of stromal interaction molecule 1 inhibits SMMC7721 hepatocellular carcinoma cell proliferation by inducing cell cycle arrest

    Suppression of stromal interaction molecule 1 inhibits SMMC7721 hepatocellular carcinoma cell proliferation by inducing cell cycle arrest

    The colony-forming ability of SMMC7721 cells is inhibited upon STIM1 lentiviral shRNA treatment. (A) Lentivirus-mediated downregulation of STIM1 suppressed the colony-forming ability of SMMC7721 cells. The cells were stained with Giemsa solution and visualized. Representative images of three independent experiments are shown. (B) The number of colonies were calculated and statistically analyzed. Cells treated with lentivirus-mediated shRNA targeting STIM1 made less clone number compared with negative control shRNA. Results represent the means ± SD of three independent experiments (* represents P < 0.05). (C) Lentivirus-mediated downregulation of STIM1 reduced the cell number per colony of SMMC7721 cells. Representative colonies of SMMC7721 cells are shown. (D) The cell number in GFP-labeled colonies were calculated and statistically analyzed. Downregulation of STIM1 inhibited the cell number in per SMMC7721 cell colony. Results represent the means ± SD of 10 random colonies (* represents P < 0.05).

  • Photocurrent generation by adsorption of two main pigments of Halobacterium salinarum on TiO2 nanostructured electrode

    Photocurrent generation by adsorption of two main pigments of Halobacterium salinarum on TiO2 nanostructured electrode

    Schematic diagram of a DSSC using two bacterial pigments (bacterioruberin and bR).

  • Optimization of pH values to formulate the bireagent kit for serum uric acid assay

    Optimization of pH values to formulate the bireagent kit for serum uric acid assay

    Effects of pH on color development during peroxidase-coupled uricase reaction. (A) Change of absorbance at 546 nm since the mixing of Reagent A, a sample in 5% BSA, and Reagent B. (B) Change of absorbance at 546 nm since the mixing of Reagent A, a sample in 5% BSA, and Reagent B, with different bireagent kits after storage at 4 °C for 3 months .

  • Overexpression of artemisinic aldehyde Δ11 (13) reductase gene–enhanced artemisinin and its relative metabolite biosynthesis in transgenic Artemisia annua L.
  • Production, optimization, and partial purification of lipase from Pseudomonas sp. strain BUP6, a novel rumen bacterium characterized from Malabari goat
  • High‐yield production of apoplast‐directed human adenosine deaminase in transgenic tobacco BY‐2 cell suspensions
  • Immobilization of lipase on porous monodisperse chitosan microspheres
  • Suppression of stromal interaction molecule 1 inhibits SMMC7721 hepatocellular carcinoma cell proliferation by inducing cell cycle arrest
  • Photocurrent generation by adsorption of two main pigments of Halobacterium salinarum on TiO2 nanostructured electrode
  • Optimization of pH values to formulate the bireagent kit for serum uric acid assay

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A chimeric protein encompassing hepatitis C virus epitopes is able to elicit both humoral and cell-mediated immune responses in mice
Daylen Aguilar-Noriega, Liz Alvarez-Lajonchere, Emma Brown, Felix Leonardo Santana, Jean Dubuisson, Czeslaw Wychowski, Ivis Guerra1, Gillian Martínez-Donato, Angel Pérez, Yalena Amador-Cañizares and Santiago Dueñas-Carrera
Volume 61, Issue 6

Infection by the hepatitic C virus is a serious problem for human health and ti date there are vaccines available against this pathogen. This work describes a chimeric protein (Eq1) encompassing hepatitic C virus amino acid regions from structural antigens expressed in bacterial. Eq1 was found to be recognized by anti- hepatitic C human sera and to be immunogenic in mice.

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