Biotechnology and Applied Biochemistry

Cover image for Vol. 61 Issue 2

Edited By: G. Gilardi and J.J. Zhong

Online ISSN: 1470-8744

Associated Title(s): Biochemistry and Molecular Biology Education, BioFactors, IUBMB Life

Featured

  • A novel biomaterial: bacterial cellulose and its new era applications

    A novel biomaterial: bacterial cellulose and its new era applications

    Increase in number of publications on BC since the 1950s compared with publications in the field of cellulose and biopolymer. (Source: http://www.ncbi.nlm.nih.gov/pubmed/?term=bacterial±cellulose/cellulose/biopolymer, accessed February 2013.)

  • Analysis of differentially expressed proteins in late-stationary growth phase of Mycobacterium tuberculosisH37Rv

    Analysis of differentially expressed proteins in late‐stationary growth phase of Mycobacterium tuberculosisH37Rv

    2-DE proteome map of Mycobacterium tuberculosisH37Rv.

  • Functional and transcriptomic analysis of the regulation of osteoblasts by mechano-growth factor E peptide

    Functional and transcriptomic analysis of the regulation of osteoblasts by mechano‐growth factor E peptide

    Microarray-base gene expression analysis of osteoblasts in response to MGF-Ct24E for 24 H. Gene expression profiles of osteoblasts were compared between the control and the MGF-Ct24E group. (A) Number and percentage of genes differentially expressed. (B) The significant GO terms of the differentially expressed genes (biological process). (C) The significant pathways of t of the differentially expressed genes. (D) Comparison of quantitative RT-PCR analysis and microarray data.

  • Amylase enzyme from Bacillus subtilis S8-18: A potential desizing agent from the marine environment

    Amylase enzyme from Bacillus subtilis S8‐18: A potential desizing agent from the marine environment

    (A) SEM images showing the desizing efficiency of commercial α-amylase (a), α-amylase from synthetic medium (b), and α-amylase from economical medium (c). Control represents the fabric samples treated with water and treated represents the fabric samples treated with respective α-amylase enzymes. (B) FTIR spectrograph of control, commercial enzyme, synthetic enzyme, and economical (rice husk) enzyme-treated fabric samples.

  • A novel ∼34-kDa α-amylase from psychrotroph Exiguobacterium sp. SH3: Production, purification, and characterization

    A novel ∼34‐kDa α‐amylase from psychrotroph Exiguobacterium sp. SH3: Production, purification, and characterization

    SDS-PAGE analysis of Amy SH3 purification steps. Lane 1: marker, lane 2: crude enzyme precipitated from culture supernatant using two volumes of acetone, lane 3: dialyzed crude enzyme, lane 4: Amy SH3 after purification using DEAE-Sepharose, Z: zymogram of purified enzyme.

  • Enhanced production of Monacolin K by addition of precursors and surfactants in submerged fermentation of Monascus purpureus 9901

    Enhanced production of Monacolin K by addition of precursors and surfactants in submerged fermentation of Monascus purpureus 9901

    Effect of different precursors on biomass and MK production in submerged fermentation of M. purpureus 9901. Conditions: Precursors of 1.0 g/L were added at the beginning of fermentation and the fermentation processes were conducted for 20 day; Values (mean ± standard deviation, n = 3) within each column having different lowercase/capital letters have significant difference (ANOVA Tukey's test; P < 0.05).

  • Thermal stability and activity improvements of a Ca-independent α-amylase from Bacillus subtilisCN7 by C-terminal truncation and hexahistidine-tag fusion

    Thermal stability and activity improvements of a Ca‐independent α‐amylase from Bacillus subtilisCN7 by C‐terminal truncation and hexahistidine‐tag fusion

    Comparative analysis of theoretical structures of α-amylases Amy7M, Amy7D, Amy7C, and Amy7N. Molecular modeling was performed by I-TASSER server (http://zhanglab.ccmb.med.umich.edu/I-TASSER/) using the 3D structure of B. subtilis α-amylase (1BAG) complexed with maltopentaose as the primary template. All the structure representations were drawn by using the PyMOL program (http://pymol.sourceforge.net/). The domain A (residues 1–96 and 151–345), domain B (residues 97–150), domain C (residues 346–422), domain D (residues 423–510), and domain E (residues 511–615) are indicated by red, black, blue, purple, and green circles, respectively, and the maltopentaose is represented by gray and red spheres. The catalytic triad residues D174, E206, and D267 of Amy7M, Amy7D, Amy7C, and Amy7N are shown in dark blue, purple, yellow, and cyan spheres, respectively. (A) Model of Amy7M. (B) Model of Amy7D. (C) Superimposition of main chains of domain A to C between Amy7M (green) and Amy7D (red). (D) Superimposition of main chains of Amy7D (red), Amy7C (yellow), and Amy7N (light gray). The N and C termini are shown by blue and red arrows, and the helix α8 located between the N- and C-parts is indicated by a green arrow. The hydrophilic his6-tag is represented in blue Connolly surface format.

  • A novel biomaterial: bacterial cellulose and its new era applications
  • Analysis of differentially expressed proteins in late‐stationary growth phase of Mycobacterium tuberculosisH37Rv
  • Functional and transcriptomic analysis of the regulation of osteoblasts by mechano‐growth factor E peptide
  • Amylase enzyme from Bacillus subtilis S8‐18: A potential desizing agent from the marine environment
  • A novel ∼34‐kDa α‐amylase from psychrotroph Exiguobacterium sp. SH3: Production, purification, and characterization
  • Enhanced production of Monacolin K by addition of precursors and surfactants in submerged fermentation of Monascus purpureus 9901
  • Thermal stability and activity improvements of a Ca‐independent α‐amylase from Bacillus subtilisCN7 by C‐terminal truncation and hexahistidine‐tag fusion

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Ras/PKA signal transduction pathway participates in the regulation of Saccharomyces cerevisiae cell apoptosis in an acidic environment
Eglė Lastauskienė, Auksė Zinkevičienė and Donaldas Čitavičius
Volume 61, Issue 1
In multicellular organisms, apoptosis plays an important role during the development and response to different environmental conditions, and many features of aging and apoptosis are conserved between yeast and multicellular organisms. Acetic acid is known as the inducer of apoptosis in the yeast Saccharomyces cerevisiae. This study showed that hydrochloric acid could also induce apoptosis in the yeast cells, and apoptotic phenotype triggered with hydrochloric acid was modulated by the Ras/PKA pathway. The activation of the Ras/PKA pathway by insertion of Ras2Val19 allele or deletion of PDE2 gene was demonstrated to increase the cell death displaying the markers of apoptosis in acid environment. Downregulation of the pathway by deletions of the RAS2, RAS1, PDE1, and insertion of the Ha-ras gene increased the cell viability and diminished the cell death with the apoptotic phenotypes. Modulations in the Ras/PKA pathway affected the cell viability and apoptosis during natural gradual acidification of the medium as well as in acid stress conditions.

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The 1st Congress of Baltic Microbiologists

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 Baltic Microbiologists

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Original Articles Available Online:

Purification and characterization of a hydrolysis-resistant lipase from Aspergillus terreus

Hui Shi, Yao Meng, Min Yang, Qinglian Zhang and Yanfa Meng

Effect of cross-linkers in fabrication of carrageenan–alginate matrices for tissue engineering application

Saet Byul Ki, Deepti Singh, Seong Cheol Kim, Tae Won Son and Sung Soo Han

A novel ∼34-kDa α-amylase from psychrotroph Exiguobacterium sp. SH3: Production, purification, and characterization

Leila Mojallali, Hossein Shahbani Zahiri, Sarah Rajaei, Kambiz Akbari Noghabi and Kamahldin Haghbeen

Expression of codon optimized human bone morphogenetic protein 4 in Pichia pastoris

Yide Huang, Binqiong Zhen, Yao Lin, Yanhui Cai, Zhen Lin, Chunmei Deng and Yanding Zhang

Mutational analysis of conserved regions harboring catalytic triad residues of the levansucrase protein encoded by the lsc-3 gene (lsc3) of Pseudomonas syringae pv. tomato DC3000

Karin Mardo, Triinu Visnapuu, Heiki Vija, Triin Elmi and Tiina Alamäe

Cross-linked gelatin microspheres with continuously tunable degradation profiles for renal tissue regeneration

Monica A. Serban, Toyin Knight, Richard G. Payne, Joydeep Basu, Elias A. Rivera, Neil Robbins, Darell McCoy, Craig Halberstadt, Deepak Jain and Timothy A. Bertram

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