Biotechnology and Applied Biochemistry

Cover image for Vol. 61 Issue 6

Edited By: G. Gilardi and Jian-Jiang Zhong

Impact Factor: 1.322

ISI Journal Citation Reports © Ranking: 2013: 119/165 (Biotechnology & Applied Microbiology); 247/291 (Biochemistry & Molecular Biology)

Online ISSN: 1470-8744

Associated Title(s): Biochemistry and Molecular Biology Education, BioFactors, IUBMB Life

Featured

  • Cloning and characterization of a novel β-carotene hydroxylase gene from Lycium barbarum and its expression in Escherichia coli

    Cloning and characterization of a novel β‐carotene hydroxylase gene from Lycium barbarum and its expression in Escherichia coli

    Nucleotide sequence and deduced amino acid sequence for the chyb cloned from Lycium barbarum.

  • Preparation and evaluation of the effect of Fe3O4@piroctone olamine magnetic nanoparticles on matrix metalloproteinase-2: A preliminary in vitro study

    Preparation and evaluation of the effect of Fe3O4@piroctone olamine magnetic nanoparticles on matrix metalloproteinase‐2: A preliminary in vitro study

    TEM micrographs of chemically synthesized (a) Fe3O4NPs and (b) Fe3O4@PO NPs.

  • A chimeric protein encompassing hepatitis C virus epitopes is able to elicit both humoral and cell-mediated immune responses in mice

    A chimeric protein encompassing hepatitis C virus epitopes is able to elicit both humoral and cell‐mediated immune responses in mice

    Expression of chimeric protein Eq1, encompassing hepatitis C virus epitopes from structural antigens. SDS/PAGE (15% gel) (A) and Western blots with anti-P64K monoclonal antibody (B) and mAb SS-HepC.1 (C). Lanes: WP, molecular weight marker; 1, GC366 cells; 2, pM238-E2 clon 1; 3, E1.340 protein; 4, Co.120 protein; 5, pM238-E2 clon 2; 6, plasmid pIME64K clon 2; 7, pM-E2E1 clon 1; 8, pM-E2E1 clon 2; 9, pM-E2E1 clon 3.

  • Comparison of modification of a bacterial uricase with N-hydroxysuccinimide esters of succinate and carbonate of monomethoxyl poly(ethylene glycol)

    Comparison of modification of a bacterial uricase with N‐hydroxysuccinimide esters of succinate and carbonate of monomethoxyl poly(ethylene glycol)

    SDS-PAGE analysis after PEGylation at 6 °C in 0.20 M sodium borate at pH 9.2 with SC-mPEG5k at the molar ratio of 200 to uricase subunits. Lanes 1–5: the use of 30 μM oxonate, the use of 100 μM xanthine, the use of 800 μM uric acid, the use of no ligands, and the native uricase alone.

  • Anti-senescence effect of FatI gene in goat somatic cells

    Anti‐senescence effect of FatI gene in goat somatic cells

    Selection and detection of FatI positive cell colonies. (A) Boer goat fibroblasts transfected with pcDNA3.1 FatI/SalI or pEGFP-NI/AseI, and nontransgenic goat fibroblasts as control. (B) Positive clones were detected by genomic DNA PCR. Lane 1 (M), DNA molecular weight marker DL2000; lane 2: negative control without DNA template; lane 3, FatI transfected cells. (C and D) The expression of FatI gene in mRNA level from FatI transfected cells, EGFP-NI transfected cells, and nontransgenic cell control were confirmed by RT-qPCR analysis.

  • Corncob hydrolysate, an efficient substrate for Monascus pigment production through submerged fermentation

    Corncob hydrolysate, an efficient substrate for Monascus pigment production through submerged fermentation

    Time profiles of biomass, residual monosaccharides, citrinin, and Monascus pigment levels in glucose medium (A) and corncob hydrolysate medium (B).

  • Effect of cultural conditions on antrodin C production by basidiomycete Antrodia camphorata in solid-state fermentation

    Effect of cultural conditions on antrodin C production by basidiomycete Antrodia camphorata in solid‐state fermentation

    Effect of interaction of soybean meal and inoculum density on antrodin C production.

  • Cloning and characterization of a novel β‐carotene hydroxylase gene from Lycium barbarum and its expression in Escherichia coli
  • Preparation and evaluation of the effect of Fe3O4@piroctone olamine magnetic nanoparticles on matrix metalloproteinase‐2: A preliminary in vitro study
  • A chimeric protein encompassing hepatitis C virus epitopes is able to elicit both humoral and cell‐mediated immune responses in mice
  • Comparison of modification of a bacterial uricase with N‐hydroxysuccinimide esters of succinate and carbonate of monomethoxyl poly(ethylene glycol)
  • Anti‐senescence effect of FatI gene in goat somatic cells
  • Corncob hydrolysate, an efficient substrate for Monascus pigment production through submerged fermentation
  • Effect of cultural conditions on antrodin C production by basidiomycete Antrodia camphorata in solid‐state fermentation

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Characterization of the 5′ flanking region of lipase gene from Penicillium expansum and its application in molecular breeding
Tian Zhang, Ying Peng, Qingsheng Yu, Jieliang Wang and Kexuan Tang.
Volume 61, Issue 5

A major challenge for further promotion of lipase productivity in Penicillium expansum is to find a suitable promoter for this industrial strain. This work characterized the 5' flanking region of P. expansum lipase (Ppel) containing a putative novel promoter sequence by fusing to β-glucuronidase (GUS), which was subsequently introduced into P. expansum. The transformants, which showed blue color quickly after incubation in GUS detection buffer, implied a strong promoter activity of this fragment. Facilitated by this novel promoter, P. expansum PE-12 was genetically modified, and the highest lipase yield reached 2,100 U/mL, which is over twofold that of a previous industrial strain. The engineered strain showed great potential in lipase industry.

IUBMB Joint Virtual Issue on Cancer Therapies

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We’re pleased to present a new Joint Virtual Issue on Cancer Therapies in celebration of the IUBMB 2015 Miami Winter Symposium, featuring articles from Biofactors, Biotechnology andApplied Biochemistry, and IUBMB Life.

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