Biotechnology and Applied Biochemistry

Cover image for Vol. 62 Issue 3

Edited By: G. Gilardi and Jian-Jiang Zhong

Impact Factor: 1.362

ISI Journal Citation Reports © Ranking: 2014: 117/162 (Biotechnology & Applied Microbiology); 245/289 (Biochemistry & Molecular Biology)

Online ISSN: 1470-8744

Associated Title(s): Biochemistry and Molecular Biology Education, BioFactors, IUBMB Life

Featured

  • The effects of endothelial progenitor cells on rat atherosclerosis

    The effects of endothelial progenitor cells on rat atherosclerosis

    Characterization of EPCs derived from rat BM in vitro. (A) Monolayer of EPCs with a cobblestone morphology after 7–10 days in culture. (B) Wright's Giemsa staining of EPCs. (C) Uptake of acetylated LDL. (D) Binding to FITC-labeled lectin. Immunofluorescence staining with anti-rat vWF, CD31, and CD144 antibody was positive in EPCs (E, F, and G, respectively) and negative in the negative control (H, fibroblast). Scale bar: 50 μm for panels (A–C) and (E–H); 25 μm for panel (D).

  • Composite cryogels for lysozyme purification

    Composite cryogels for lysozyme purification

    SDS-PAGE image of chicken egg white protein fractions stained with silver nitrate. Lane 1: chicken egg white solution after adsorption. Lane 2: chicken egg white solution before adsorption. Lane 3: desorbed Lyz. Lane 4: bovine serum albumin (BSA) and Lyz (standard proteins from Sigma).

  • Effect of physicochemical parameters on nitrile-hydrolyzing potentials of newly isolated nitrilase of Fusarium oxysporum f. sp. lycopercisi ED-3

    Effect of physicochemical parameters on nitrile‐hydrolyzing potentials of newly isolated nitrilase of Fusarium oxysporum f. sp. lycopercisi ED‐3

    Formation of pink color around microbial culture in pH-based minimal salt media shows the presence of nitrilase activity of the strains. Bacterial strain of Rhodococcus rhodochrous was taken as positive control for this method.

  • Adsorption-based immobilization of Caldicellulosiruptor saccharolyticus cellobiose 2-epimerase on Bacillus subtilis spores

    Adsorption‐based immobilization of Caldicellulosiruptor saccharolyticus cellobiose 2‐epimerase on Bacillus subtilis spores

    Effect of pH on the activity of free and immobilized CsCE. The activities obtained at pH 7.5 for free and pH 7.0 for immobilized enzyme were taken as 100%.

  • High production of succinyl isoflavone glycosides by Bacillus licheniformis ZSP01 resting cells in aqueous miscible organic medium

    High production of succinyl isoflavone glycosides by Bacillus licheniformis ZSP01 resting cells in aqueous miscible organic medium

    Conversion of daidzein, daidzin, genistein, and genistin from soybean flour extract to corresponding succinyl products by resting cells of B. licheniformis ZSP01. The substrate used in the reaction system was soybean flour extract (6.1%, w/v), and other reaction conditions were the same as those described in Fig. .

  • Optimization of medium composition for 3-hydroxycarboxylic acid production by Pseudomonas mendocina–biodegraded polyhydroxybutyrate

    Optimization of medium composition for 3‐hydroxycarboxylic acid production by Pseudomonas mendocina–biodegraded polyhydroxybutyrate

    Three-dimensional and plots response surface showing the effects of variables (PHB, NH4Cl, and KH2PO4) on the response (yield of 3-HB).

  • Enhanced production of d-lactic acid by Sporolactobacillus sp.Y2–8 mutant generated by atmospheric and room temperature plasma

    Enhanced production of d‐lactic acid by Sporolactobacillus sp.Y2–8 mutant generated by atmospheric and room temperature plasma

    The fermentation curves of YBS1–5 and Y2–8 in 5 L bioreactor with fed-batch strategy. The values given are the average of three independent experiments. ●, Glucose of the Y2–8; ▪, glucose of the YBS1–5; ○, d-lactic acid of the Y2–8; □, d-lactic acid of the YBS1–5.

  • The effects of endothelial progenitor cells on rat atherosclerosis
  • Composite cryogels for lysozyme purification
  • Effect of physicochemical parameters on nitrile‐hydrolyzing potentials of newly isolated nitrilase of Fusarium oxysporum f. sp. lycopercisi ED‐3
  • Adsorption‐based immobilization of Caldicellulosiruptor saccharolyticus cellobiose 2‐epimerase on Bacillus subtilis spores
  • High production of succinyl isoflavone glycosides by Bacillus licheniformis ZSP01 resting cells in aqueous miscible organic medium
  • Optimization of medium composition for 3‐hydroxycarboxylic acid production by Pseudomonas mendocina–biodegraded polyhydroxybutyrate
  • Enhanced production of d‐lactic acid by Sporolactobacillus sp.Y2–8 mutant generated by atmospheric and room temperature plasma

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A chimeric protein encompassing hepatitis C virus epitopes is able to elicit both humoral and cell-mediated immune responses in mice
Daylen Aguilar-Noriega, Liz Alvarez-Lajonchere, Emma Brown, Felix Leonardo Santana, Jean Dubuisson, Czeslaw Wychowski, Ivis Guerra1, Gillian Martínez-Donato, Angel Pérez, Yalena Amador-Cañizares and Santiago Dueñas-Carrera
Volume 61, Issue 6

Infection by the hepatitic C virus is a serious problem for human health and ti date there are vaccines available against this pathogen. This work describes a chimeric protein (Eq1) encompassing hepatitic C virus amino acid regions from structural antigens expressed in bacterial. Eq1 was found to be recognized by anti- hepatitic C human sera and to be immunogenic in mice.

IUBMB Joint Virtual Issue on Cancer Therapies

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We’re pleased to present a new Joint Virtual Issue on Cancer Therapies in celebration of the IUBMB 2015 Miami Winter Symposium, featuring articles from Biofactors, Biotechnology andApplied Biochemistry, and IUBMB Life.

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