Biotechnology and Applied Biochemistry

Cover image for Vol. 61 Issue 4

Edited By: G. Gilardi and J.J. Zhong

Impact Factor: 1.322

ISI Journal Citation Reports © Ranking: 2013: 119/165 (Biotechnology & Applied Microbiology); 247/291 (Biochemistry & Molecular Biology)

Online ISSN: 1470-8744

Associated Title(s): Biochemistry and Molecular Biology Education, BioFactors, IUBMB Life

Featured

  • Blood protein repulsion after peptide entrapment in pendant polyethylene oxide layers

    Blood protein repulsion after peptide entrapment in pendant polyethylene oxide layers

    Relative adsorption of FITC–fibrinogen onto uncoated (hydrophobic TCVS) and PEO-coated, 1-μm silica microspheres (top). Visible yellow tint (bottom) indicates adsorption of FITC–fibrinogen onto microsphere surfaces.

  • The preparation of VEGFR1/CD3 bispecific antibody and its specific cytotoxicity against VEGFR1-positive breast cancer cells

    The preparation of VEGFR1/CD3 bispecific antibody and its specific cytotoxicity against VEGFR1‐positive breast cancer cells

    The proliferation assay of PBLs induced by VEGFR1/CD3 BsAb. PBLs were stimulated by VEGFR1/CD3 BsAb (0, 10, 100, 1,000 ng/mL) or OKT3 (100 ng/mL) for 72 H, and the 450 nm absorption was measured after the incubation of 4 H with CCK-8 at 37 °C. The proliferation rate of PBLs was calculated statistically and presented in histogram. Values are expressed as means ± SD from three independent experiments performed in triplicate. *P < 0.05. **P < 0.01 versus control.

  • Catalytic and synergistic antibacterial potential of green synthesized silver nanoparticles: Their ecotoxicological evaluation on Poecillia reticulata

    Catalytic and synergistic antibacterial potential of green synthesized silver nanoparticles: Their ecotoxicological evaluation on Poecillia reticulata

    (A) Zone of inhibition images of S. aureus with antibiotics alone and (B) antibiotics impregnated with AgNPs. (C) Closer view of the zone of inhibition by penicillin alone and (D) penicillin with biosynthesized AgNPs against E. coli.

  • Rhamsan gum production by Sphingomonas sp. CGMCC 6833 using a two-stage agitation speed control strategy

    Rhamsan gum production by Sphingomonas sp. CGMCC 6833 using a two‐stage agitation speed control strategy

    Time course of rhamsan gum production at different agitation speeds: 400 rpm (triangles), 600 rpm (filled circles), 800 rpm (squares), and 1,000 rpm (filled triangles). (A) Biomass, (B) glucose, (C) rhamsan gum, and (D) viscosity.

  • Purification and characterization of arylacetonitrile-specific nitrilase of Alcaligenes sp. MTCC 10675

    Purification and characterization of arylacetonitrile‐specific nitrilase of Alcaligenes sp. MTCC 10675

    Multiple sequence alignment of the amino acid sequence of arylaceionitrilase of Alcaligenes sp. MTCC 10675 and already reported nitrilase.

  • Significant expression of a Chinese scorpion peptide, BmK1, in Escherichia coli through promoter engineering and gene dosage strategy

    Significant expression of a Chinese scorpion peptide, BmK1, in Escherichia coli through promoter engineering and gene dosage strategy

    Codon optimization and expression cassette construction. (A) DNA sequence alignment between original and optimized BmK1 gene. (B) The entire open reading frame of the recombinant BmK1 gene.

  • Blood protein repulsion after peptide entrapment in pendant polyethylene oxide layers
  • The preparation of VEGFR1/CD3 bispecific antibody and its specific cytotoxicity against VEGFR1‐positive breast cancer cells
  • Catalytic and synergistic antibacterial potential of green synthesized silver nanoparticles: Their ecotoxicological evaluation on Poecillia reticulata
  • Rhamsan gum production by Sphingomonas sp. CGMCC 6833 using a two‐stage agitation speed control strategy
  • Purification and characterization of arylacetonitrile‐specific nitrilase of Alcaligenes sp. MTCC 10675
  • Significant expression of a Chinese scorpion peptide, BmK1, in Escherichia coli through promoter engineering and gene dosage strategy

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Characterization of the 5′ flanking region of lipase gene from Penicillium expansum and its application in molecular breeding
Tian Zhang, Ying Peng, Qingsheng Yu, Jieliang Wang and Kexuan Tang.
Volume 61, Issue 5

A major challenge for further promotion of lipase productivity in Penicillium expansum is to find a suitable promoter for this industrial strain. This work characterized the 5' flanking region of P. expansum lipase (Ppel) containing a putative novel promoter sequence by fusing to β-glucuronidase (GUS), which was subsequently introduced into P. expansum. The transformants, which showed blue color quickly after incubation in GUS detection buffer, implied a strong promoter activity of this fragment. Facilitated by this novel promoter, P. expansum PE-12 was genetically modified, and the highest lipase yield reached 2,100 U/mL, which is over twofold that of a previous industrial strain. The engineered strain showed great potential in lipase industry.

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Efficient production of fermentable sugars from oil palm empty fruit bunch by combined use of acid and whole cell culture-catalyzed hydrolyses
Qingxin Li, Wei Ting Ng, Sze Min Puah, Ravindran Vijay Bhaskar, Loon Siong Soh, Calum MacBeath, Pius Parakattil, Phil Green, Jin Chuan Wu

A novel method for the immoblization of a thermostable fungal chitinase and the properties of the immobilized enzyme
Muthu Prasad, Peramachi Palanivelu

Ethanol production using a newly isolated Saccharomyces cerevisiae strain directly assimilating intact inulin with a high degree of polymerization
Fan Yang, Zhicheng Liu, Weifeng Dong, Linghuan Zhu, Xiaoyi Chen, Xianzhen Li

Blood protein repulsion after peptide entrapment in pendant polyethylene oxide layers
Julie A. Auxier, Justen K. Dill, Karl F. Schilke, Joseph McGuire

Rhamsan gum production by Sphingomonas sp. CGMCC 6833 using a two-stage agitation speed control strategy
Xiao Ying Xu, Ping Zhu, Sha Li, Xiao Ye Chen, Xing Huan Jiang, Hong Xu

15th IUBMB - 24th FAOBMB - TSBMB International Conference, Taipei, Taiwan

Biochemistry and Molecular Biology in Transition: from Basic to Translational

15th IUBMB - 24th FAOBMB - TSBMB International Conference

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