Biotechnology and Applied Biochemistry

Cover image for Vol. 61 Issue 1

Edited By: G. Gilardi and J.J. Zhong

Online ISSN: 1470-8744

Associated Title(s): Biochemistry and Molecular Biology Education, BioFactors, IUBMB Life

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  • Mutational analysis of conserved regions harboring catalytic triad residues of the levansucrase protein encoded by the lsc-3 gene (lsc3) of Pseudomonas syringae pv. tomato DC3000

    Mutational analysis of conserved regions harboring catalytic triad residues of the levansucrase protein encoded by the lsc‐3 gene (lsc3) of Pseudomonas syringae pv. tomato DC3000

    Predicted structure of Lsc3 protein of P. syringae pv. tomato. Lsc3 was modeled in ModWeb server as in Ref. [] using Arthrobacter sp. K-1 β-fructofuranosidase (PDB ID: 3vss) as a template. Conservation of positions in scale from 1 (variable) to 9 (fully conserved) was calculated by ConSurf (see Section ) and amino acids on Lsc3 model were colored accordingly. PyMol (the PyMol Molecular Graphics System, Version 1.3, Schrodinger, LLC, Munich, Germany) was used for structure visualization. (A) A surface mode of predicted 3D model of Lsc3 with catalytic triad residues indicated in yellow and fructose residue from 3vss structure shown in catalytic pocket. (B) Closer insight of the active site pocket. Amino acids with major effect on catalysis (comprising catalytic triad) are highlighted by yellow color and presented as sticks: Asp62 (nucleophile), Asp219 (transition-state stabilizer), Glu303 (acid-base catalyst). A color version of this figure is presented in the online article.

  • Characterization of the bacterioplankton community and its antibiotic resistance genes in the Baltic Sea

    Characterization of the bacterioplankton community and its antibiotic resistance genes in the Baltic Sea

    The composition of the bacterial community of the Baltic Sea by phylum in 2008 and 2009.

  • Effects of yeast immobilization on bioethanol production

    Effects of yeast immobilization on bioethanol production

    SEM image of immobilized yeast cells on (A) the hydroxylapatite surface and (B) the chamotte surface. The scale bar is 20 μm.

  • Ras/PKA signal transduction pathway participates in the regulation of Saccharomyces cerevisiae cell apoptosis in an acidic environment

    Ras/PKA signal transduction pathway participates in the regulation of Saccharomyces cerevisiae cell apoptosis in an acidic environment

    (A) Confocal laser scanning microscopy picture of Ras2Val19 cells after 78 H of growth in SC medium shows various morphological changes in the nucleus structure: 1, normal nucleus; 2, fragmented nucleus; 3, no visible nucleus (diffused nucleus); 4, double nucleus. (B) Percentage of the cells with abnormal nucleus after 78 H of Ras2Val19 cell growth in SC medium.

  • Interaction of Lactobacillus vini with the ethanol-producing yeasts Dekkera bruxellensis and Saccharomyces cerevisiae

    Interaction of Lactobacillus vini with the ethanol‐producing yeasts Dekkera bruxellensis and Saccharomyces cerevisiae

    Coflocculation of L. vini with yeasts during batch cocultivation. (A) L. vini and S. cerevisiae, (B) L. vini and D. bruxellensis. (Magnification ×400.)

  • Adaptation of Dekkera bruxellensis to lignocellulose-based substrate

    Adaptation of Dekkera bruxellensis to lignocellulose‐based substrate

    Expression of ADH1 (A) and ADH2 (B) genes in adapted (black columns) and nonadapted (grey columns) D. bruxellensis after 0, 8, and 40 H of cultivation in lignocellulose hydrolysate-derived medium (expressed as ratio of mRNA copy numbers of ADH genes to ACT1).

  • Ex vivo cytokine production in peripheral blood mononuclear cells after their stimulation with dsRNA of natural origin

    Ex vivo cytokine production in peripheral blood mononuclear cells after their stimulation with dsRNA of natural origin

    Detected changes in concentration of molecules with early response type in the presence of Larifan. The Y axis shows the concentration of molecules (mean of two replicate values with standard deviation), pg/mL; the X axis shows the analyzed time point, hours.

  • Mutational analysis of conserved regions harboring catalytic triad residues of the levansucrase protein encoded by the lsc‐3 gene (lsc3) of Pseudomonas syringae pv. tomato DC3000
  • Characterization of the bacterioplankton community and its antibiotic resistance genes in the Baltic Sea
  • Effects of yeast immobilization on bioethanol production
  • Ras/PKA signal transduction pathway participates in the regulation of Saccharomyces cerevisiae cell apoptosis in an acidic environment
  • Interaction of Lactobacillus vini with the ethanol‐producing yeasts Dekkera bruxellensis and Saccharomyces cerevisiae
  • Adaptation of Dekkera bruxellensis to lignocellulose‐based substrate
  • Ex vivo cytokine production in peripheral blood mononuclear cells after their stimulation with dsRNA of natural origin

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Ras/PKA signal transduction pathway participates in the regulation of Saccharomyces cerevisiae cell apoptosis in an acidic environment
Eglė Lastauskienė, Auksė Zinkevičienė and Donaldas Čitavičius
Volume 61, Issue 1
In multicellular organisms, apoptosis plays an important role during the development and response to different environmental conditions, and many features of aging and apoptosis are conserved between yeast and multicellular organisms. Acetic acid is known as the inducer of apoptosis in the yeast Saccharomyces cerevisiae. This study showed that hydrochloric acid could also induce apoptosis in the yeast cells, and apoptotic phenotype triggered with hydrochloric acid was modulated by the Ras/PKA pathway. The activation of the Ras/PKA pathway by insertion of Ras2Val19 allele or deletion of PDE2 gene was demonstrated to increase the cell death displaying the markers of apoptosis in acid environment. Downregulation of the pathway by deletions of the RAS2, RAS1, PDE1, and insertion of the Ha-ras gene increased the cell viability and diminished the cell death with the apoptotic phenotypes. Modulations in the Ras/PKA pathway affected the cell viability and apoptosis during natural gradual acidification of the medium as well as in acid stress conditions.

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The 1st Congress of Baltic Microbiologists

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 Baltic Microbiologists

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Original Articles Available Online:

Purification and characterization of a hydrolysis-resistant lipase from Aspergillus terreus

Hui Shi, Yao Meng, Min Yang, Qinglian Zhang and Yanfa Meng

Effect of cross-linkers in fabrication of carrageenan–alginate matrices for tissue engineering application

Saet Byul Ki, Deepti Singh, Seong Cheol Kim, Tae Won Son and Sung Soo Han

A novel ∼34-kDa α-amylase from psychrotroph Exiguobacterium sp. SH3: Production, purification, and characterization

Leila Mojallali, Hossein Shahbani Zahiri, Sarah Rajaei, Kambiz Akbari Noghabi and Kamahldin Haghbeen

Expression of codon optimized human bone morphogenetic protein 4 in Pichia pastoris

Yide Huang, Binqiong Zhen, Yao Lin, Yanhui Cai, Zhen Lin, Chunmei Deng and Yanding Zhang

Mutational analysis of conserved regions harboring catalytic triad residues of the levansucrase protein encoded by the lsc-3 gene (lsc3) of Pseudomonas syringae pv. tomato DC3000

Karin Mardo, Triinu Visnapuu, Heiki Vija, Triin Elmi and Tiina Alamäe

Cross-linked gelatin microspheres with continuously tunable degradation profiles for renal tissue regeneration

Monica A. Serban, Toyin Knight, Richard G. Payne, Joydeep Basu, Elias A. Rivera, Neil Robbins, Darell McCoy, Craig Halberstadt, Deepak Jain and Timothy A. Bertram

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