Biotechnology and Applied Biochemistry
Copyright © 2014 International Union of Biochemistry and Molecular Biology, Inc.
Study of the effect of F17A mutation on characteristics of Bacillus thermocatenulatus lipase expressed in Pichia pastoris using in silico and experimental methods
Continuous ethanol production from sugarcane molasses using a newly designed combined bioreactor system by immobilized Saccharomyces cerevisiae
Cloning and in vitro function analysis of codon-optimized FatI gene
Effect of uracil addition on proteomic profiles and 1,3-β-glucan production in Agrobacterium sp.
Effect of membranes on oxygen transfer rate and consumption within a newly developed three-compartment bioartificial liver device: Advanced experimental and theoretical studies
Development of an immuno-based colorimetric assay for white spot syndrome virus
Immunohistochemical characterization of novel murine monoclonal antibodies against human placenta-specific 1
Recently Published Issues
Engineering the MEP pathway enhanced ajmalicine biosynthesis
Kai Chang1, Fei Qiu, Min Chen, Lingjiang Zeng, Xiaoqiang Liu, Chunxian Yang, Xiaozhong Lan, Qiang Wang, and Zhihua Liao.
Volume 61, Issue 3
The terpenoid indole alkaloids (TIAs) consisting of the terpenoid moiety and the indole moiety include many high-value-added pharmaceutical alkaloids such as ajmalicine, vinblastine, and vincristine. The terpenoid moiety is provided by the plastidial 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway (nonmevalonate pathway) and the indole moiety by the tryptamine pathway. Catharanthus roseus is the model plant to unveil biosynthesis of TIAs at the molecular and biochemical levels. Up to now, the reported metabolic engineering of TIA biosynthesis mainly focused on genetic modification of the indole moiety biosynthetic pathway and the strictosidine-after pathway. This study did the genetic modification of the MEP pathway by overexpressing DXR and MECS in hairy root cultures of C. roseus, and the effect of overexpressing STR combined with DXR and MECS was also discussed. The results indicated that the transgenic hairy root cultures with co-overexpression of DXR and STR or MECS and STR had higher levels of ajmalicine than those with overexpression of a single gene alone such as DXR, MECS, and STR.
Make Your Article More Discoverable Online
Top Tips to Make Your Article More Discoverable Online:
- Make sure you have a SEO-friendly title for your article.
- Carefully craft your abstract using keywords, keywords, keywords
- Provide at least five keywords of phrases in the keywords field
- Stay consistent when referring to authors' names and initials throughout your article and make sure you are referring to them in the same way they have been referred to in past online publications
- Use headings for the various sections of your article to tip off search engines to the structure and content of your article. Incorporate your keywords and phrases in these headings wherever it is appropriate.
- Cite your own, or your co-authors, previous publications as appropriate
Click here for more information, an example of a well-optimized abstract, and tips on promoting your article after publication using the internet and social media.
Click below to read these Original Articles in Early View:
Efficient production of fermentable sugars from oil palm empty fruit bunch by combined use of acid and whole cell culture-catalyzed hydrolyses
Qingxin Li, Wei Ting Ng, Sze Min Puah, Ravindran Vijay Bhaskar, Loon Siong Soh, Calum MacBeath, Pius Parakattil, Phil Green, Jin Chuan Wu
A novel method for the immoblization of a thermostable fungal chitinase and the properties of the immobilized enzyme
Muthu Prasad, Peramachi Palanivelu
Ethanol production using a newly isolated Saccharomyces cerevisiae strain directly assimilating intact inulin with a high degree of polymerization
Fan Yang, Zhicheng Liu, Weifeng Dong, Linghuan Zhu, Xiaoyi Chen, Xianzhen Li
Blood protein repulsion after peptide entrapment in pendant polyethylene oxide layers
Julie A. Auxier, Justen K. Dill, Karl F. Schilke, Joseph McGuire
Rhamsan gum production by Sphingomonas sp. CGMCC 6833 using a two-stage agitation speed control strategy
Xiao Ying Xu, Ping Zhu, Sha Li, Xiao Ye Chen, Xing Huan Jiang, Hong Xu
Become a 'Friend of the IUBMB'
The International Union of Biochemistry and Molecular Biology seeks to advance the international molecular life sciences community by promoting interactions across the diversity of endeavors in the molecular life sciences; creating networks that transcend barriers of ethnicity, culture, gender and economic status; creating pathways for young scientists to fulfil their potential; providing evidence based advice on public policy; and promoting the values, standards and ethics of science and the free and unhampered movement of scientists of all nations.
Register to become a Friend of the IUBMB and you will will receive no more than one short email each month to keep you up to date about the IUBMB's international activities and what the Union can offer you, such as fellowships, support for meetings, advanced schools, and more!!