Biotechnology and Applied Biochemistry

PfaH2: A novel hydrophobin from the ascomycetePaecilomyces farinosus
Katerina Zelena, Meike Takenberg, Stefan Lunkenbein, Susanne K. Woche, Manfred Nimtz, Ralf G. Berger
Volume 60, Issue 2

Fungi produce unique proteins such as lignocellulolytic enzymes and hydrophobins. Hydrophobins have potential applications such as in long-term stabilization of foams and for biomaterials and medical applications. In this work, the pfah2 gene coding for the novel hydrophobin PfaH2 from the ascomycete Paecilomyces farinosus was identified. The protein was isolated using trifluoroacetic acid extraction and purified via SDS-PAGE and high-performance liquid chromatography. Its surface activity was compared with that of a recently described PfaH1. Surprisingly, the two hydrophobins adsorbed to hydrophilic surfaces and changed their physicochemical properties to similar extents, although little amino-acid sequence homology was found. READ MORE...

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The special issue is opened by a review by Tsuneo Omura, one of the pioneers of cytochrome P450 enzymology, where the key role played by these enzymes in the evolution from prokaryotic to eukaryotic cells and their importance in de- fence mechanisms are discussed. The volume reports on the discoveries of new bacterial P450s from Pseudomonas putida (Wong and coworkers), Sorangium cellulosum (Bernhardt and coworkers), key new findings on the role of structure and con- formations in deep sea bacteria, as well as spectroscopic studies related to substrate binding in bacterial P450s (Davydov and coworkers).

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