Biotechnology and Applied Biochemistry

Cover image for Vol. 62 Issue 6

Edited By: G. Gilardi and Jian-Jiang Zhong

Impact Factor: 1.362

ISI Journal Citation Reports © Ranking: 2014: 118/163 (Biotechnology & Applied Microbiology); 246/290 (Biochemistry & Molecular Biology)

Online ISSN: 1470-8744

Associated Title(s): Biochemistry and Molecular Biology Education, BioFactors, IUBMB Life

Featured

  • Knockdown of GPR137 by RNAi inhibits pancreatic cancer cell growth and induces apoptosis

    Knockdown of GPR137 by RNAi inhibits pancreatic cancer cell growth and induces apoptosis

    Knockdown of GPR137 induced cell apoptosis. (A) Cytogram of annexin V–APC binding versus 7-AAD uptake in BXPC-3 cells following lentivirus infection in all three groups (Con, Lv-shCon, and Lv-shGPR137). (B) Quantification of apoptotic cells in BXPC-3 cells by FACS (***P < 0.001). Annexin V–/7-AAD–: viable cells, annexin V–/7-AAD+: cells in necrosis, annexin V–/7-AAD–: early apoptotic cells, annexin V+/7-AAD+: late apoptotic cells. (C) Western blot analysis of PARP and caspase 3 expression in BXPC-3 cells.

  • The use of steam explosion to increase the nutrition available from rice straw

    The use of steam explosion to increase the nutrition available from rice straw

    Response surface plot for the effects of pressure {A} and retention time {B} (in code number) on the yield of reducing sugar.

  • Optimization of nanofibrous silk fibroin scaffold as a delivery system for bone marrow adherent cells: in vitro and in vivo studies

    Optimization of nanofibrous silk fibroin scaffold as a delivery system for bone marrow adherent cells: in vitro and in vivo studies

    Disk diffusion results of the ESFNS-10% against yeast (a), P. aeruginosa (ATCC 27833) (b), S. aureus (ATCC 25923) (c), and E. coli (ATCC 25922) (d). The growth inhibition zone around the ESFNS disks was not seen in all the samples, indicating no antimicrobial activity of the ESFNS. Black dash indicates the size of disk placed on the plate (indicating 1 cm in diameter).

  • Innovative approach for urease inhibition by Ficus carica extract–fabricated silver nanoparticles: An in vitro study

    Innovative approach for urease inhibition by Ficus carica extract–fabricated silver nanoparticles: An in vitro study

    Characterization of AgNPs: UV–Vis spectroscopy (inset: eppendorf tubes showing color change in AgNPs after incubation at different time intervals with F. carica leaves extract).

  • Molecular cloning and characterization of a novel carotenoid cleavage dioxygenase 1 from Lycium chinense

    Molecular cloning and characterization of a novel carotenoid cleavage dioxygenase 1 from Lycium chinense

    Alignment of the predicted amino acid sequence of the LmCCD1 and the CCD1 proteins from other plants. Arabidopsis thaliana (AtCCD1, AJ005813); Vitis vinifera (VvCCD1, KF008001); Zea mays (ZmCCD1, DQ100346); Lycopersicon esculentum (LeCCD1A, AY576001). “” indicates the four histidine residues.

  • Chebulin: Terminalia chebula Retz. fruit-derived peptide with angiotensin-I–converting enzyme inhibitory activity

    Chebulin: Terminalia chebula Retz. fruit‐derived peptide with angiotensin‐I–converting enzyme inhibitory activity

    The complex structure models of chebulin with (a) porcine ACE and (b) human ACE. Chebulin structure is shown in olive color (a) and green color (b) stick form, and the binding residues of porcine and human ACE are shown in blue violet color (a) and dark red color (b) stick form. Asp-1 of chebulin forms H bonds with Asp-330 and Tyr-370 of the porcine ACE, and Phe-6 of chebulin forms hydrophobic interaction with Trp-251, Leu-483, Phe-492, and Tyr-495 of the porcine ACE (a). Lys-5, Asn-3, Ser-4, Asp-1, Asp-1, and Asn-3 of chebulin form H bonds with Glu-162, Ala-356, His-383, Glu-403, Arg-522, and Tyr-523 of the human ACE (b).

  • Selection by phage display of nanobodies directed against hypoxia inducible factor-1α (HIF-1α)

    Selection by phage display of nanobodies directed against hypoxia inducible factor‐1α (HIF‐1α)

    Heavy-chain antibodies (HCAbs) and variable domain of the heavy chain of HCAbs, VHH. Camelid hcAbs contain only two heavy chains, which are composed of one variable (VHH) and two constant (CH2 and CH3) domains lacking the CH1 domain. The VHH is the minimal intact antigen-binding fragment that can be generated.

  • Knockdown of GPR137 by RNAi inhibits pancreatic cancer cell growth and induces apoptosis
  • The use of steam explosion to increase the nutrition available from rice straw
  • Optimization of nanofibrous silk fibroin scaffold as a delivery system for bone marrow adherent cells: in vitro and in vivo studies
  • Innovative approach for urease inhibition by Ficus carica extract–fabricated silver nanoparticles: An in vitro study
  • Molecular cloning and characterization of a novel carotenoid cleavage dioxygenase 1 from Lycium chinense
  • Chebulin: Terminalia chebula Retz. fruit‐derived peptide with angiotensin‐I–converting enzyme inhibitory activity
  • Selection by phage display of nanobodies directed against hypoxia inducible factor‐1α (HIF‐1α)

Recently Published Issues

See all

Editor's Choice

Read More

A chimeric protein encompassing hepatitis C virus epitopes is able to elicit both humoral and cell-mediated immune responses in mice
Daylen Aguilar-Noriega, Liz Alvarez-Lajonchere, Emma Brown, Felix Leonardo Santana, Jean Dubuisson, Czeslaw Wychowski, Ivis Guerra1, Gillian Martínez-Donato, Angel Pérez, Yalena Amador-Cañizares and Santiago Dueñas-Carrera
Volume 61, Issue 6

Infection by the hepatitic C virus is a serious problem for human health and ti date there are vaccines available against this pathogen. This work describes a chimeric protein (Eq1) encompassing hepatitic C virus amino acid regions from structural antigens expressed in bacterial. Eq1 was found to be recognized by anti- hepatitic C human sera and to be immunogenic in mice.

News

BAB app

 



We’re pleased to present a new Joint Virtual Issue on Cancer Therapies in celebration of the IUBMB 2015 Miami Winter Symposium, featuring articles from Biofactors, Biotechnology andApplied Biochemistry, and IUBMB Life.

Become a 'Friend of the IUBMB'

Friends of the IUBMB

NA

The International Union of Biochemistry and Molecular Biology seeks to advance the international molecular life sciences community by promoting interactions across the diversity of endeavors in the molecular life sciences; creating networks that transcend barriers of ethnicity, culture, gender and economic status; creating pathways for young scientists to fulfil their potential; providing evidence based advice on public policy; and promoting the values, standards and ethics of science and the free and unhampered movement of scientists of all nations.

Register to become a Friend of the IUBMB and you will will receive no more than one short email each month to keep you up to date about the IUBMB's international activities and what the Union can offer you, such as fellowships, support for meetings, advanced schools, and more!!

SEARCH

SEARCH BY CITATION