Biotechnology and Applied Biochemistry

Cover image for Vol. 63 Issue 2

Edited By: G. Gilardi and Jian-Jiang Zhong

Impact Factor: 1.362

ISI Journal Citation Reports © Ranking: 2014: 118/163 (Biotechnology & Applied Microbiology); 246/290 (Biochemistry & Molecular Biology)

Online ISSN: 1470-8744

Associated Title(s): Biochemistry and Molecular Biology Education, BioFactors, IUBMB Life

Featured

  • An improved method for the production of fructooligosaccharides by immobilized β-fructofuranosidase from Sclerotinia sclerotiorum

    An improved method for the production of fructooligosaccharides by immobilized β‐fructofuranosidase from Sclerotinia sclerotiorum

    Purification of extracellular β-fructofuranosidase (FFase) from Sclerotinia sclerotiorum. (a) Gel filtration chromatography of the crude enzyme preparation. (b) DEAE-cellulose chromatography profile of FFase. One fraction contained 2 mL; (●) protein, (Δ) enzyme activity, and (–) NaCl gradient. (c) SDS-PAGE and zymography of extracellular FFase from S. sclerotiorum. M, molecular mass markers (kDa); lane 1, crude extract; lane 2, proteins purified by gel filtration; lane 3, proteins purified by DEAE–cellulose chromatography; lane 4, zymography of the pooled anion–exchange chromatography fractions.

  • Isolation and characterization of novel multifunctional recombinant family 26 glycoside hydrolase from Mehsani buffalo rumen metagenome

    Isolation and characterization of novel multifunctional recombinant family 26 glycoside hydrolase from Mehsani buffalo rumen metagenome

    Close-up view of the ligand-binding site for GH26 enzyme. Residues that directly interact with the substrate are shown as sticks. Images were generated by the PyMOL software.

  • Molecular cloning and expression of a new α-neoagarobiose hydrolase from Agarivorans gilvus WH0801 and enzymatic production of 3,6-anhydro-l-galactose

    Molecular cloning and expression of a new α‐neoagarobiose hydrolase from Agarivorans gilvus WH0801 and enzymatic production of 3,6‐anhydro‐l‐galactose

    SDS-PAGE of the recombinant AgaWH117 overexpressed in E. coli BL21 (DE3). Lane M, protein MW marker (Fermentas). Lane 1, AgaWH117 purified by His-tag affinity chromatography. Lane 2, supernatant fraction of overexpressed AgaWH117 before purification.

  • Preparation, characterization, and silanization of 3D microporous PDMS structure with properly sized pores for endothelial cell culture

    Preparation, characterization, and silanization of 3D microporous PDMS structure with properly sized pores for endothelial cell culture

    The morphology of endothelial cells cultured on the PDMS sponge after 48 H of cell seeding: (a and b) magnification 1,000× and (c and d) magnification 2,000×.

  • High-throughput microarray mapping of cell wall polymers in roots and tubers during the viscosity-reducing process

    High‐throughput microarray mapping of cell wall polymers in roots and tubers during the viscosity‐reducing process

    Comprehensive microarray polymer profiling (CoMPP) of Shangshu 19, cassava, and Canna edulis Ker. during the entire viscosity-reducing process. The heatmap represents the total intensity for CDTA and NaOH extractions. The numbers in the heatmap matrix indicate the signal intensity (0[RIGHTWARDS ARROW]150, signal from low (black color) to high (green color)). Twenty-six types of mAbs and CBMs (Table ) were applied to map the cell wall polymers of roots and tubers, and 15 types of mAbs and CBMs have signals according to this heatmap. ME: methyl-esterified.

  • Expression and characterization of bifunctional fusion proteins possessing antitumor and thrombolytic function for targeting therapy

    Expression and characterization of bifunctional fusion proteins possessing antitumor and thrombolytic function for targeting therapy

    Determination of lymphocyte proliferation of fusion proteins by the MTT assay. Positive control, ConA (5 μg/ mL); negative control, BSA (50 ng/ mL). All values are averaged from five independent experiments, n = 5, x ± SEM. Compared with negative control group: P < 0.01.

  • An improved method for the production of fructooligosaccharides by immobilized β‐fructofuranosidase from Sclerotinia sclerotiorum
  • Isolation and characterization of novel multifunctional recombinant family 26 glycoside hydrolase from Mehsani buffalo rumen metagenome
  • Molecular cloning and expression of a new α‐neoagarobiose hydrolase from Agarivorans gilvus WH0801 and enzymatic production of 3,6‐anhydro‐l‐galactose
  • Preparation, characterization, and silanization of 3D microporous PDMS structure with properly sized pores for endothelial cell culture
  • High‐throughput microarray mapping of cell wall polymers in roots and tubers during the viscosity‐reducing process
  • Expression and characterization of bifunctional fusion proteins possessing antitumor and thrombolytic function for targeting therapy

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A chimeric protein encompassing hepatitis C virus epitopes is able to elicit both humoral and cell-mediated immune responses in mice
Daylen Aguilar-Noriega, Liz Alvarez-Lajonchere, Emma Brown, Felix Leonardo Santana, Jean Dubuisson, Czeslaw Wychowski, Ivis Guerra1, Gillian Martínez-Donato, Angel Pérez, Yalena Amador-Cañizares and Santiago Dueñas-Carrera
Volume 61, Issue 6

Infection by the hepatitic C virus is a serious problem for human health and ti date there are vaccines available against this pathogen. This work describes a chimeric protein (Eq1) encompassing hepatitic C virus amino acid regions from structural antigens expressed in bacterial. Eq1 was found to be recognized by anti- hepatitic C human sera and to be immunogenic in mice.

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