Biotechnology and Applied Biochemistry
Copyright © 2014 International Union of Biochemistry and Molecular Biology, Inc.
Study of the effect of F17A mutation on characteristics of Bacillus thermocatenulatus lipase expressed in Pichia pastoris using in silico and experimental methods
Continuous ethanol production from sugarcane molasses using a newly designed combined bioreactor system by immobilized Saccharomyces cerevisiae
Cloning and in vitro function analysis of codon-optimized FatI gene
Effect of uracil addition on proteomic profiles and 1,3-β-glucan production in Agrobacterium sp.
Effect of membranes on oxygen transfer rate and consumption within a newly developed three-compartment bioartificial liver device: Advanced experimental and theoretical studies
Development of an immuno-based colorimetric assay for white spot syndrome virus
Immunohistochemical characterization of novel murine monoclonal antibodies against human placenta-specific 1
Recently Published Issues
Engineering the MEP pathway enhanced ajmalicine biosynthesis
Kai Chang1, Fei Qiu, Min Chen, Lingjiang Zeng, Xiaoqiang Liu, Chunxian Yang, Xiaozhong Lan, Qiang Wang, and Zhihua Liao.
Volume 61, Issue 3
The terpenoid indole alkaloids (TIAs) consisting of the terpenoid moiety and the indole moiety include many high-value-added pharmaceutical alkaloids such as ajmalicine, vinblastine, and vincristine. The terpenoid moiety is provided by the plastidial 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway (nonmevalonate pathway) and the indole moiety by the tryptamine pathway. Catharanthus roseus is the model plant to unveil biosynthesis of TIAs at the molecular and biochemical levels. Up to now, the reported metabolic engineering of TIA biosynthesis mainly focused on genetic modification of the indole moiety biosynthetic pathway and the strictosidine-after pathway. This study did the genetic modification of the MEP pathway by overexpressing DXR and MECS in hairy root cultures of C. roseus, and the effect of overexpressing STR combined with DXR and MECS was also discussed. The results indicated that the transgenic hairy root cultures with co-overexpression of DXR and STR or MECS and STR had higher levels of ajmalicine than those with overexpression of a single gene alone such as DXR, MECS, and STR.
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The 1st Congress of Baltic Microbiologists
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