Biotechnology and Applied Biochemistry

Cover image for Vol. 63 Issue 4

Edited By: G. Gilardi and Jian-Jiang Zhong

Impact Factor: 1.429

ISI Journal Citation Reports © Ranking: 2015: 118/161 (Biotechnology & Applied Microbiology); 240/289 (Biochemistry & Molecular Biology)

Online ISSN: 1470-8744

Associated Title(s): Biochemistry and Molecular Biology Education, BioFactors, IUBMB Life

Featured

  • Enzyme-resistant isomalto-oligosaccharides produced from Leuconostoc mesenteroides NRRL B-1426 dextran hydrolysis for functional food application

    Enzyme‐resistant isomalto‐oligosaccharides produced from Leuconostoc mesenteroides NRRL B‐1426 dextran hydrolysis for functional food application

    PAGE analysis of partially purified dextransucrase (PEG-400; 33%, v/v) from L. mesenteroides NRRL B-1426. (a) Silver staining of SDS-PAGE under reducing and non-reducing conditions, Lanes: M, protein molecular weight marker: 10–200 kDa (Fermentas, Thermo scientific); 1, reducing; 2, non-reducing; (b) PAS staining of dextransucrase under non-reducing condition; 3, sucrose; 4, raffinose; 5, raffinose treated with dextranase; and (c) native-PAGE analysis of dextransucrase.

  • Improvement of biomass production and glucoamylase activity by Candida famata using factorial design

    Improvement of biomass production and glucoamylase activity by Candida famata using factorial design

    Contour plots and response surface curves of GA (GA) from C. famata showing interactions between pH value and time of fermentation (A, B), interactions between pH value and starch concentration (C, D), and interactions between time of fermentation and starch concentration (E, F).

  • Optimization of a heat-tolerant β-glucosidase production by Bacillus sp. ZJ1308 and its purification and characterization

    Optimization of a heat‐tolerant β‐glucosidase production by Bacillus sp. ZJ1308 and its purification and characterization

    Three-dimensional response surface plots and 2D contour plots showing the effect of corn cob (X1), beef extract (X2), and MnSO4·H2O (X3) on the activity of β-glucosidase. A: Y = f(X1, X2), C: Y = f(X1, X3), and E: Y = f(X2, X3).

  • Enzymatic transformation of ginseng leaf saponin by recombinant β-glucosidase (bgp1) and its efficacy in an adipocyte cell line

    Enzymatic transformation of ginseng leaf saponin by recombinant β‐glucosidase (bgp1) and its efficacy in an adipocyte cell line

    The effect of normal and bioconverted ginseng leaf saponins on 3T3-L1 adipocyte differentiation. The measurement of the cytotoxic activity of leaf saponins and transformed leaf saponins at different concentrations was performed by MTT assay after treatment for 48 H (A). Lipid content was visualized by Oil Red O staining on day 8 of differentiation at the concentrations of 1, 10, and 100 μg/mL (C), followed by lipid content evaluation by absorbance measurement (B). The data showed statistical significance ***P < 0.0005 between the control and positive control groups, while ***P < 0.0005 was noted between positive and treated groups. TLS: transformed leaf saponin; LS: leaf saponin; PC: positive control.

  • Titanium dioxide nanoparticles provide protection against polycyclic aromatic hydrocarbon BaP and chrysene-induced perturbation of DNA repair machinery: A computational biology approach

    Titanium dioxide nanoparticles provide protection against polycyclic aromatic hydrocarbon BaP and chrysene‐induced perturbation of DNA repair machinery: A computational biology approach

    Binding sites of AHR–BAP complex (Pro180, Ser181, Cys183, Gly187, Leu196, Val200, Asn204, Leu259, Pro260, Leu265, Ala269, Thr270, Leu272, and Pro274).

  • Assessing the stereoselectivity of carbonyl reductases toward the reduction of OPBE and docking analysis

    Assessing the stereoselectivity of carbonyl reductases toward the reduction of OPBE and docking analysis

    (A) Structural observation of SeCR–NADPH–OPBE model. (B) Structural insights into the SeCR–NADPH–OPBE model and key residues involved in substrate catalysis. The bound NADPH is highlighted in stick and colored in yellow. The catalytic tetrad residues are highlighted in stick and colored in magenta. Substrate OPBE is colored in green.

  • Enzyme‐resistant isomalto‐oligosaccharides produced from Leuconostoc mesenteroides NRRL B‐1426 dextran hydrolysis for functional food application
  • Improvement of biomass production and glucoamylase activity by Candida famata using factorial design
  • Optimization of a heat‐tolerant β‐glucosidase production by Bacillus sp. ZJ1308 and its purification and characterization
  • Enzymatic transformation of ginseng leaf saponin by recombinant β‐glucosidase (bgp1) and its efficacy in an adipocyte cell line
  • Titanium dioxide nanoparticles provide protection against polycyclic aromatic hydrocarbon BaP and chrysene‐induced perturbation of DNA repair machinery: A computational biology approach
  • Assessing the stereoselectivity of carbonyl reductases toward the reduction of OPBE and docking analysis

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A chimeric protein encompassing hepatitis C virus epitopes is able to elicit both humoral and cell-mediated immune responses in mice
Daylen Aguilar-Noriega, Liz Alvarez-Lajonchere, Emma Brown, Felix Leonardo Santana, Jean Dubuisson, Czeslaw Wychowski, Ivis Guerra1, Gillian Martínez-Donato, Angel Pérez, Yalena Amador-Cañizares and Santiago Dueñas-Carrera
Volume 61, Issue 6

Infection by the hepatitic C virus is a serious problem for human health and ti date there are vaccines available against this pathogen. This work describes a chimeric protein (Eq1) encompassing hepatitic C virus amino acid regions from structural antigens expressed in bacterial. Eq1 was found to be recognized by anti- hepatitic C human sera and to be immunogenic in mice.

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