Chemical Evolution of a Bacterial Proteome
Dr. Michael Georg Hoesl, M. Sc. Stefan Oehm, Dr. Patrick Durkin, Dr. Elise Darmon, Dr. Lauri Peil, Dr. Hans-Rudolf Aerni, Prof. Dr. Juri Rappsilber, Prof. Dr. Jesse Rinehart, Prof. Dr. David Leach, Prof. Dr. Dieter Söll and Prof. Dr. Nediljko Budisa
Article first published online: 23 JUL 2015 | DOI: 10.1002/anie.201506522
A first step towards the creation of synthetic cells is described by N. Budisa et al. in their Communication on (DOI: 10.1002/anie.201502868). The chemical composition and the genetic code of an auxotrophic E. coli were changed in the frame of a long-term evolution experiment. During the experiment the cells were gradually forced to replace the natural building block tryptophan with thienopyrrolylalanine in their proteomes. This is the first step in the creation of synthetic life, which should be genetically and metabolically so far away from that found in nature that it cannot survive outside of the laboratory.