Acetylcholinesterase-Catalyzed Hydrolysis Allows Ultrasensitive Detection of Pathogens with the Naked Eye
Dingbin Liu, Zhantong Wang, Dr. Albert Jin, Xinglu Huang, Xiaolian Sun, Fu Wang, Qiang Yan, Shengxiang Ge, Prof. Ningshao Xia, Gang Niu, Gang Liu, Dr. A. R. Hight Walker and Dr. Xiaoyuan Chen
Article first published online: 11 DEC 2013 | DOI: 10.1002/anie.201309874
A new colorimetric assay for pathogen detection is described by X. Chen et al. in their Communication (DOI: 10.1002/anie.201307952). An improved heterogeneous sandwich-type ELISA enhances detection sensitivity by a two-step signal amplification process involving gold particle aggregation induced by acetylcholinesterase-catalyzed hydrolysis, which leads to a color change, followed by enrichment of the analyte using magnetic beads. This assay is as sensitive as RT-PCR, but more convenient and less expensive.