An Accurate In Vitro Model of the E. coli Envelope
Dr. Luke A. Clifton, Dr. Stephen A. Holt, Dr. Arwel V. Hughes, Dr. Emma L. Daulton, Dr. Wanatchaporn Arunmanee, Dr. Frank Heinrich, Dr. Syma Khalid, Damien Jefferies, Dr. Timothy R. Charlton, Dr. John R. P. Webster, Dr. Christian J. Kinane and Prof. Jeremy H. Lakey
Article first published online: 1 SEP 2015 | DOI: 10.1002/anie.201504287
Understanding the outer membranes of Gram-negative bacteria is important for the development of new antibacterial compounds. However, their structure and dynamics are poorly understood because of their small in vivo size and inaccurate in vitro models. A stable asymmetric model of the outer membrane that can be analyzed by a range of biophysical techniques and accurately imitates the in vivo behavior of natural outer membranes is herein presented.