Angewandte Chemie International Edition
Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Inside Cover: Stereospecific Isotopic Labeling of Methyl Groups for NMR Spectroscopic Studies of High-Molecular-Weight Proteins (Angew. Chem. Int. Ed. 11/2010)
NMR spectroscopy of large protein assemblies necessitates targeted protonation of specific sites within a perdeuterated background. In their Communication on page 1958 ff., J. Boisbouvier et al. outline the use of an acetolactate precursor for the specific biosynthetic incorporation of 13C1H3 into the pro-S methyl groups of leucine and valine. This labeling strategy enhances spectral quality and provides an efficient basis for the application of solution-state NMR techniques to complex biomolecules.