Angewandte Chemie International Edition
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Back Cover: Template-Directed Synthesis in 3'- and 5'-Direction with Reversible Termination (Angew. Chem. Int. Ed. 33/2012)
Simultaneous chemical primer extension in both 3'- and 5'-direction is reported by C. Richert et al. in their Communication on page 8299 ff. The use of magnetic beads as a solid support and azidomethyloxycarbonyl moieties as protecting groups provided control over the extension steps directed by a DNA sequence. The method combines features of replication and of automated DNA synthesis.