Angewandte Chemie International Edition
Copyright © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Back Cover: A Programmable Sensor to Probe the Internalization of Proteins and Nanoparticles in Live Cells (Angew. Chem. Int. Ed. 22/2013)
The internalization of proteins and nanoparticles in living cells can be quantified by using a new DNA nanosensor, as described by A. P. R. Johnston and H. Liu in their Communication on page 5744 ff. The material of interest is labeled with a fluorescent DNA probe, and the fluorescence of any material that remains on the surface of the cell is quenched upon the addition of a quencher probe. The fluorescence of internalized material and other fluorophores is unaffected. This technique enables multi-color assays and studies in primary cells without compromising sensitivity or quantification.