Angewandte Chemie International Edition

Cover image for Vol. 54 Issue 21

Editor: Peter Gölitz, Deputy Editors: Neville Compton, Haymo Ross

Online ISSN: 1521-3773

Associated Title(s): Angewandte Chemie, Chemistry - A European Journal, Chemistry – An Asian Journal, ChemistryOpen, ChemPlusChem, Zeitschrift für Chemie

52_22b/2013Back Cover: A Programmable Sensor to Probe the Internalization of Proteins and Nanoparticles in Live Cells (Angew. Chem. Int. Ed. 22/2013)

The internalization of proteins and nanoparticles in living cells can be quantified by using a new DNA nanosensor, as described by A. P. R. Johnston and H. Liu in their Communication on page 5744 ff. The material of interest is labeled with a fluorescent DNA probe, and the fluorescence of any material that remains on the surface of the cell is quenched upon the addition of a quencher probe. The fluorescence of internalized material and other fluorophores is unaffected. This technique enables multi-color assays and studies in primary cells without compromising sensitivity or quantification.

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