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August 10, 2009
Functional Polymers: Heparin Quantification
Jianbing Shi, Kan-Yi Pu, Ruoyu Zhan, Bin Liu*
Heparin is a biological molecule, which plays an important role in a number of physiological processes, such as cell growth and differentiation, and is widely used as an injectable anticoagulant to prevent thrombosis. However, a heparin overdose can induce thrombocytopenia, which is recognized as one of the most catastrophic complications of heparin treatment, hence making exact heparin sensing a highly critical issue. Bin Liu and co-workers present the synthesis of a cationic conjugated polymer containing 20 mol-% 2,1,3-benzothiadiazole (BT) which exhibits a good water solubility. The formation of interpolyelectrolyte complexes between the oppositely charged polymer and heparin facilitates energy transfer from the fluorene segments to the BT units within the polymer, leading to an intensity increase for the orange emission (585 nm) at the expense of the blue emission intensity at 412 nm. A practical calibration curve covering the whole range of the therapeutic dosing level of heparin (0.2 to 76 x 10-6 M) is thus obtained by correlating the changes in BT emission with the heparin concentration in buffer.
Macromol. Chem. Phys., DOI: 10.1002/macp.200900080
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