© WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
December 16, 2010
Stem Cells: Agarose-Alginate Cryogel for Bioengineering Applications
Anuj Tripathi and Ashok Kumar*
Agarose and alginate are two natural polysaccharides which are biodegradable, biocompatible, inert, and have soft tissue like mechanical properties. Therefore, they possess great potential in bioengineering applications. Here, agarose-alginate (AA) scaffolds are synthesized using the cryogelation technology in different formats like monolith, sheet, discs, and beads. In cell-material interactions, fibroblast (NIH-3T3) cells show good adherence and proliferation on these scaffolds presenting its potential application in soft tissue engineering. Further experimental results indicate that the AA cryogel might be used as a support matrix for cell immobilization and as a filter to remove heavy metals from wastewater. These cryogels do have a number of unique features that make them an important class of soft materials for developing multi-featured scaffolds as a novel carrier for bioengineering applications.
Macromol. Biosci., DOI: 10.1002/mabi.201000286