© WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
February 17, 2011
Stem Cells: Moving Gradient Generation to the Third Dimension
Eric H. Nguyen, Michael P. Schwartz, and William L. Murphy*
Soluble concentration gradients such as growth factor gradients play a critical role in controlling tissue formation during embryonic development. The critical importance of soluble signaling in biology has motivated numerous studies aimed at modeling gradient generation in vitro. Many previous studies have applied soluble signaling gradients to cells in two-dimensional (2D) culture. Techniques for the generation of these gradients within three-dimensional biomaterial scaffolds are just beginning to emerge. When applied to tissue engineering applications, biomimetic morphogen gradients may offer an unprecedented level of control over tissue development. This review presents emerging toolsets that lend themselves to the generation of these gradients.
Macromol. Biosci., DOI: 10.1002/mabi.201000448
Other contributions to the article series on stem cells can be found here.