© WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
March 16, 2011
Stem Cells: ECM Functionalized Microcavities to Control Hematopoietic Stem and Progenitor Cell Fate
Ina Kurth, Katja Franke, Tilo Pompe,* Martin Bornhäuser, and Carsten Werner
Bone marrow (BM) constitutes a specialized microenvironment supporting hematopoietic stem and progenitor cell (HSPC) homeostasis, which is known as "stem cell niche". Even though HSPC have already been studied for more than three decades the complexity of the BM-HSPC interplay impedes the full understanding of stem cell properties and their destiny. Tile Pompe and co-workers use now polymeric microcavities functionalized with extracellular matrix (ECM) components as an experimental in vitro model to investigate principles of HSPC fate control. Using human CD133 + HSPC they could demonstrate distinct differences in HSPC cycling and differentiation dependence on the adhesion ligand specificity (i.e., heparin, collagen I) and cytokine levels. The presented microcavity platform provides a powerful in vitro approach to explore the role of exogenous cues in HSPC fate decisions and can therefore be instrumental to progress in stem cell biology and translational research toward new therapies.
Macromol. Biosci., DOI: 10.1002/mabi.201000432
Other contributions to the article series on stem cells can be found here.