© WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
April 05, 2011
Stem Cells: A Tool for Studying Stem Cells during Wound-Healing Processes
Annalisa Grimaldi,* Serena Banfi, Jacopo Vizioli, Gianluca Tettamanti, Douglas M. Noonan, and Magda de Eguileor
Matrigel (MG), a basement membrane extract, is an injectable biopolymer that can be used for implanting cells, providing improved incorporation in host tissues. MG loaded with cytokine can be used for the recruitment in vivo of specific cell populations and as a vector for the preparation of cell cultures. A. Grimaldi and co-workers demonstrate that the injection of MG supplemented with interleukin-8 (IL-8) in the leech Hirudo medicinalis can be used to purify cell populations showing the same morpho-functional and molecular mechanisms of specific populations of vertebrate hematopoietic precursor cells involved in tissue repair. These cells spontaneously differentiated into myofibroblasts. This approach highlights how the innovative use of a cytokine-loaded biopolymer for an in vivo cell sorting method, applied to a simple invertebrate model, can be a tool for studying myofibroblast cell biology and its regulation, step by step.
Macromol. Biosci., DOI: 10.1002/mabi.201000452
Other contributions to the article series on stem cells can be found here.