© WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
October 07, 2011
Polymer Conjugates: 3D Arrangement of Short DNA Oligonucleotides at Surfaces
Olivier Y. F. Henry,* Ahmed D. Mehdi, Sinead Kirwan, Josep Luis Acero Sanchez, and Ciara K. O'Sullivan*
The need for new approaches to realize rapid and highly sensitive diagnostic devices and environmental monitoring systems requires ever more effective surface chemistries. The arrangement of short DNA oligonucleotides at surfaces plays a major role in the overall surface binding capacity of the complementary sequence present in a sample. Henry, O'Sullivan and co-workers incorporate short DNA oligonucleotide into acrylamide brushes via surface initiated ATRP in an attempt to increase the DNA surface density by building 3D molecular architectures. The morphology and the performance of the resulting polymer are characterized by surface plasmon resonance and ATR-FTIR spectroscopy, as well as MALDI-TOF analysis, and compared to monolayers of thiolated DNA strands. This new approach offers a promising alternative to SAM-based nucleic acid and aptamer sensors and could enable the realization of more complex soft materials of controlled architecture capable of both recognition and signaling by including additional optically or electrochemically active moieties.
Macromol. Rapid Commun., DOI: 10.1002/marc.201100317
Other contributions to the article series on polymer conjugates can be found here.