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December 19, 2011
Stem Cells: A Dextran-Based Polymer as Cell-Detaching Substrate
Guoming Sun, Sravanti Kusuma, and Sharon Gerecht*
In order for stem cells to be translated into clinical use, they must be treated with substances that will not harm their surface proteins and be cultured on biocompatible substrates which will not pose a threat to the patient once the cells are implanted. Sharon Gerecht and co-workers synthesized a dextran-based, biodegradable, temperature-sensitive polymer - TSDAIE - and tested it as a novel, substrate for nonenzymatic cell detachment. The concentration of TSDAIE affects endothelial progenitor cell (EPC) attachment, which is thereby used to optimize the concentration of TSDAIE for coating. At the determined optimal concentration, TSDAIE is found to be compatible for use in EPC culture as revealed by cell attachment, spreading, proliferation, and phenotype. Because of its complete biodegradability, TSDAIE can be used directly in the culture of stem cells without requiring the additional step of removing nondegradable polymer. This advantage can expedite stem cell scale-up for clinical use.
Macromol. Biosci., DOI: 10.1002/mabi.201100258
Other contributions to the article series on stem cells can be found here.