Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Editor-in-Chief: Peter Gregory, Deputy Editors: Martin Ottmar, Carolina Novo da Silva, Lorna Stimson
Online ISSN: 1521-4095
Associated Title(s): Advanced Energy Materials, Advanced Engineering Materials, Advanced Functional Materials, Advanced Healthcare Materials, Advanced Materials Interfaces, Advanced Optical Materials, Particle & Particle Systems Characterization, Small
Sensors: Real-Time, Sensitive, and Specific Detection of Promoter-Polymerase Interactions in Gene Transcription Using a Nanoplasmonic Sensor (Adv. Mater. 9/2013)
Sang Jun Sim and co-workers demonstrate on page 1265 the specific recognition of pointmutated promoter by RNA polymerase. The interactions between promoter and RNA polymerase are monitored using Rayleigh light scattering on gold nanoparticles. Using a darkfield microscope, the individual gold nanoparticles can be visualized as the bright colored spots (red, green, or orange in the cover image) by light scattering. Binding of RNA polymerase on the surface of a AuNP is quantitatively detected by LSPR (localized surface plasmon resonance) maximum wavelength shift.