Chemical Engineering & Technology
Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Every month, the Editors select two papers referring to current discussions in the scientific, public and economic communities and in view of the potential for further developments. The papers are freely accessible for one month.
Designing Printable Medicinal Products
August 05, 2014
The pharmaceutical industry is currently in a challenging situation due to escalating expenses for the development of new pharmaceuticals and declining numbers of quality pharmaceutical products reaching the market. In such a scenario, continuous manufacturing is being considered as one of the attractive solutions due to various advantages it offers, e.g., lower production cost, improvement in process yield, greater reliability, safety, and robustness. Printable medicinal products may be considered as potential future medicinal products due to a wide range of benefits they offer, such as the flexibility of delivering tailor-made doses and the opportunity for continuously operating manufacturing lines. Jukka Rantanen et al. used piroxicam as a model compound to design printable medicinal products. The investigation started with solubility screening in different solvents, followed by substrate screening. Factors to be considered for optimization of ink formulations for printing were discussed. Furthermore, they demonstrated an analytical approach using scanning electron microscopy-energy dispersive X-ray spectroscopy for imaging of the model compound in the printed medicinal products.
Dhara Raijada, Natalja Genina, Daniela Fors, Erik Wisaeus, Jouko Peltonen, Jukka Rantanen, Niklas Sandler
Designing Printable Medicinal Products: Solvent System and Carrier-Substrate Screening
Chem. Eng. Technol. 2014, 37 (8), 1291-1296.
Purification of Lysozyme
August 05, 2014
The demand for proteins and enzymes is increasing in the fields of biotechnology and medicine as well as the food industry. Crystalline proteins show superior properties compared to amorphous formulations, such as higher purity and longer shelf life. Despite the fact that the structure of proteins is more complicated, protein crystallization also follows the basic rules of crystallization. A proper supersaturation level should be obtained to induce nucleation and crystal growth. In order to create the desired supersaturation level, the current protein crystallization technologies often consume high quantities of salt as precipitant. Joachim Ulrich et al. investigated the solvent-freeze-out (SFO) technology for the separation of lysozyme from a lysozyme/crude extract protein mixture. Based on the results from the screening tests, the SFO technology could successfully be carried out for the purification of lysozyme. No contamination from the crude extract was observed and activity tests showed that the lysozyme retained its original enzymatic activity after the SFO process. The experiments indicate that the method is effective and reproducible and should be very promising when put into practice.
Xiaoxi Yu, Jingkang Wang, Joachim Ulrich
Purification of Lysozyme from Protein Mixtures by Solvent-Freeze-Out Technology
Chem. Eng. Technol. 2014, 37 (8), 1353-1357.