IUBMB Life

Cover image for Vol. 66 Issue 9

Edited By: Angelo Azzi and William J. Whelan

Impact Factor: 2.755

ISI Journal Citation Reports © Ranking: 2013: 115/185 (Cell Biology); 154/291 (Biochemistry & Molecular Biology)

Online ISSN: 1521-6551

Associated Title(s): Biochemistry and Molecular Biology Education, Biotechnology and Applied Biochemistry, BioFactors

Featured

  • Iterative type I polyketide synthases involved in enediyne natural product biosynthesis

    Iterative type I polyketide synthases involved in enediyne natural product biosynthesis

    Chronicle of PKSE product discoveries. A: Heptaene 1 was isolated from 9-membered PKSE coexpression with thioesterase in heterogeneous host (2008; 39). B: Deficient NADPH causes release of truncated polyketide 3 and methylhexaenone 2 from 10-membered PKSE catalyzed reaction with thioesterase (2008–2009; 48, 49, 51, 57). C: Without assistance of thioesterase, 9-membered enediyne SgcE produces nonaketide 4 as major product; high concentration of thioesterase SgcE10 causes release of premature intermediate 1 and 5 (2010; 53). D: In vivo coexpression of 10-membered CalE8 in heterogeneous host in dark without thioesterase produces β-hydroxy acid 5 as the major product (2012; 55). Note: 1) M-CoA, malonyl CoA; TE, thioesterase; 2) PKSE in blue: 9-membered PKSE represented by SgcE and NcsE, PKSE in orange: 10-membered eneidyne represented by CalE8 and DynE; 3) CO2 and H2O side products released from decarboxylation and dehydration are not shown for the purpose of clarity. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]

  • Bicarbonate transport in health and disease

    Bicarbonate transport in health and disease

    Pancreatic bicarbonate secretion. Pancreatic fluid has the highest HCO3− concentration of any bodily fluid, about 140 mM . To achieve this transepithelial flux, the cell is loaded with HCO3− by NBCe1 and by diffusion of CO2 into the cell, followed by CAII-mediated catalysis to HCO3− and H+. NHE1 removes H+ and loads the cell with additional Na+. Patients with cystic fibrosis have defective pancreatic HCO3− secretion, indicating a key role of CFTR in pancreatic HCO3− secretion. The role of CFTR is twofold: 1) CFTR is a Cl− channel, providing extracellular Cl− to sustain Cl−/HCO3− exchange by SLC26A6, and 2) CFTR is a HCO3− channel, mediating HCO3− efflux. Not shown in this simplified model are the ion channels/Na+/K+-ATPase required to maintain homeostasis. NBCn1 localizes to the apical membrane, but functions in HCO3− salvage, not secretion . The mechanism of pancreatic duct cell HCO3− secretion has been thoroughly reviewed .

  • Apolipoprotein E isoforms and lipoprotein metabolism

    Apolipoprotein E isoforms and lipoprotein metabolism

    Schematic comparing the influence of apoE3 and apoE4 on the lipolysis cascade involved in the catabolism of VLDL particles . After secretion from the liver into the plasma compartment, the triglyceride (TG) in VLDL is hydrolyzed by lipoprotein lipase (LPL) with apoC-II as a cofactor leading to the creation of intermediate density lipoprotein (IDL) and progressively smaller remnant particles. ApoE bound to these particles mediates their interaction with the LDLR and clearance from plasma (the lower red curved arrow shows the decrease in VLDL and remnant cholesterol levels). As the VLDL/IDL remnants shrink due to the removal of core TG, excess surface components (PL, cholesterol and apoE) are released into the HDL pool (upper blue curved arrow shows the increase in HDL cholesterol level). ApoE3 partitions between the VLDL and HDL pools so that lipolysis, clearance of VLDL remnant cholesterol and HDL formation is optimal. In the diagram, points c and d represent the VLDL/IDL-cholesterol and HDL-cholesterol levels, respectively, when apoE3 is expressed. Relative to apoE3, apoE4 binds more to VLDL because of its higher lipid affinity leading to inhibition of lipolysis (probably because of displacement of apoC-II) so that, at the same apoE expression level, progression down the lipolysis cascade is relatively limited in the case of apoE4. The ratio a/b represents the apoE4 VLDL-C/HDL-C ratio which is higher than the apoE3 ratio c/d. See text for further details. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]

  • Molecular structure and rotary dynamics of Enterococcus hiraeV1-ATPase

    Molecular structure and rotary dynamics of Enterococcus hiraeV1‐ATPase

    Single-molecule rotation assay of EhV1. A: Schematic drawing of the single-molecule rotation assay of EhV1. B: Examples of clear and unclear states in the rotation of EhV1. Left: Time-revolution plots. Center: XY plots. Right: Distribution of rotary angles. The two clear states are indicated by 1 and 3 and the one unclear state is indicated by 2. C: Distribution of the dwell times of the clear (top) and unclear (bottom) states. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]

  • Structural insights into RNA recognition properties of glyceraldehyde-3-phosphate dehydrogenase 3 from Saccharomyces cerevisiae

    Structural insights into RNA recognition properties of glyceraldehyde‐3‐phosphate dehydrogenase 3 from Saccharomyces cerevisiae

    A: Overall structure of S. cerevisiae GAPDH3. The NAD+-binding domain and catalytic domain are colored ruby and cyan, respectively. B: Spatial organization of the tetramer obtained by comparison with one homologous structure (gray, PDB code: 3LVF) and operating through three crystallographic twofold axes. The subunits A (blue), A1 (cyan), A2 (magenta), and A3 (green) correspond to the subunits O, P, Q, and R, respectively, of the homologous structure (PDB code: 3LVF). C: Schematic view of the tetramer contact surface. The interacting areas on each GAPDH3 molecule are colored red, green, blue, and deep blue, respectively. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]

  • Aquaporin-9 is expressed in rat Sertoli cells and interacts with the cystic fibrosis transmembrane conductance regulator

    Aquaporin‐9 is expressed in rat Sertoli cells and interacts with the cystic fibrosis transmembrane conductance regulator

    Model showing the molecular interaction of aquaporin-9 (AQP9) and cystic fibrosis transmembrane conductance regulator (CFTR) in cultured rat Sertoli cells (SCs) (panel A) as determined by the co-immunoprecipitation technique (panel B). Rat SCs lysates were incubated with magnetic beads functionalized with anti-CFTR antibody. Antibody-bound protein complexes containing CFTR were eluted from the magnetic beads, and proteins were identified by the Slot blot technique. Immunoprecipitated CFTR protein was detected, and coimmunoprecipitated AQP9 protein was derived from the SCs eluted (panel C). Panel C represents six replicas of the detection by the Slot blot technique of CFTR and AQP9 protein obtained after the coimmunoprecipitation technique. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]

  • MicroRNA-378 inhibits cell growth and enhances l-OHP-induced apoptosis in human colorectal cancer

    MicroRNA‐378 inhibits cell growth and enhances l‐OHP‐induced apoptosis in human colorectal cancer

    Effects of miR-378 on proliferation and cell cycle transition in HCT116 and HT29 cells. A: A colony formation assay was used to detect the colony formation activity of HCT116 and HT29 cells transfected with miR-378 mimics and controls, respectively. B: An MTT assay was performed to measure the cellular viability. The line charts show the relative MTT absorbance, which indicates the cellular viability, at 1–6 days after transfection with the miR-378 mimics and control. C: Propidium iodide staining and FCM analyses were used to detect the cell cycle distribution of HCT116 and HT29 cells. D: MiR-378 inhibits CRC cell-derived xenograft tumor growth in vivo. The left image represents the isolated tumors, and the right image is the growth curve drawn according to the tumor volume at the indicated times (*P < 0.05). [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]

  • Iterative type I polyketide synthases involved in enediyne natural product biosynthesis
  • Bicarbonate transport in health and disease
  • Apolipoprotein E isoforms and lipoprotein metabolism
  • Molecular structure and rotary dynamics of Enterococcus hiraeV1‐ATPase
  • Structural insights into RNA recognition properties of glyceraldehyde‐3‐phosphate dehydrogenase 3 from Saccharomyces cerevisiae
  • Aquaporin‐9 is expressed in rat Sertoli cells and interacts with the cystic fibrosis transmembrane conductance regulator
  • MicroRNA‐378 inhibits cell growth and enhances l‐OHP‐induced apoptosis in human colorectal cancer

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15th IUBMB - 24th FAOBMB - TSBMB International Conference, Taipei, Taiwan

Biochemistry and Molecular Biology in Transition: from Basic to Translational

15th IUBMB - 24th FAOBMB - TSBMB International Conference

2014 IUBMB Life Young Investigator Award

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On behalf of the IUBMB, IUBMB Life, and Wiley, it is with great pleasure and honor that we announce Liudmila Abrosimova as the recipient of the 2014 IUBMB Life - Wiley Young Investigator Award for her article, Thermo-switchable activity of the restriction endonuclease Ssoll achieved by site-directed enzyme modification.

Ms. Abrosimova is affiliated with the Department of Bioengineering and Bioinformatics, Department of Chemistry, and Belozersky Institute of Physico-Chemical Biology at Lomonosov Moscow State University. She will be honored with the 2014 IUBMB Life - Wiley Young Investigator Award at the 15th IUBMB - 24th FAOBMB - TSBMB Conference this October 21 - 26, in Taipei, Taiwan, and her award-winning article will be FREELY available online through the conference.

Please join us in congratulating Ms. Abrosimova as the recipient of the annual IUBMB Life – Wiley Young Investigator Award!

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