IUBMB Life

Cover image for Vol. 67 Issue 7

Edited By: Angelo Azzi and William J. Whelan

Impact Factor: 3.143

ISI Journal Citation Reports © Ranking: 2014: 102/184 (Cell Biology); 115/289 (Biochemistry & Molecular Biology)

Online ISSN: 1521-6551

Associated Title(s): Biochemistry and Molecular Biology Education, Biotechnology and Applied Biochemistry, BioFactors

Featured

  • Interleukin-1α and brain inflammation

    Interleukin‐1α and brain inflammation

    The role of IL-1α in inflammatory diseases and brain injury. Infections or sterile inflammatory stimuli induced by tissue injury or the release of DAMPs lead to the production of IL-1α in peripheral organs and cells that results diverse inflammatory changes. Activated immune cells, platelets and inflammatory mediators contribute to vascular inflammation in both the periphery and the cerebral blood vessels that includes increased expression of adhesion molecules, facilitating leukocyte and platelet recruitment and production of inflammatory mediators. IL-1α-mediated inflammation could promote acute cerebrovascular events and alter inflammatory responses in the brain after an acute cerebrovascular event. IL-1α released from activated circulating immune cells, platelets and from resident brain cells (such as microglia) could facilitate inflammation, vascular permeability and various forms of cell death (pyroptosis, apoptosis and necroptosis) in the brain leading to increased brain injury and worse outcome.

  • Fructosylation generates neo-epitopes on human serum albumin

    Fructosylation generates neo‐epitopes on human serum albumin

    DLS profile of native (A) and fructose-modified (B) HSA for determining hydrodynamic radii.

  • Alterations of TMEM16a allostery in human retinal microarterioles in long-standing hypertension

    Alterations of TMEM16a allostery in human retinal microarterioles in long‐standing hypertension

    Increased protein interactions between TMEM16A and the cytosolic calcium sensor calmodulin in retina blood vessels obtained from human subjects with chronic hypertension in comparison with retinas obtained from age-matched controls. Protein interactions were significantly decreased after preincubation in W7 and E6 berbamine (11.01 ± 1.01 vs. 26 ± 0.89 vs. 5.31 ± 0.34 vs. 6.8 ± 0.78, comparison of PLA blob spots/µm3 voxels, P < 0.01, ANOVA).

  • Treg-specific demethylated region activity in isolated regulatory t lymphocytes is a surrogate for disease severity in hepatocellular carcinoma

    Treg‐specific demethylated region activity in isolated regulatory t lymphocytes is a surrogate for disease severity in hepatocellular carcinoma

    Increased nuclear translocation of transcriptional enhancers in liver-resident Tregs obtained from patients with hepatocellular carcinoma. Nuclear translocation of STAT5 and Ets1 was examined. N, nucleus; C, cytosol; ab, primary antibody adsorbed with peptide. Positive controls for the primary protein of interest are shown in the right panel of both the lanes. Note minimal expression of STAT5 and Ets1 in nuclei of control T regulatory cells. 5-Azacytidine (5-aza) was used to induce demethylation in control cells to replicate the in vivo findings.

  • Quercetin protects necrotic insult and promotes apoptosis by attenuating the expression of RAGE and its ligand HMGB1 in human breast adenocarcinoma cells

    Quercetin protects necrotic insult and promotes apoptosis by attenuating the expression of RAGE and its ligand HMGB1 in human breast adenocarcinoma cells

    Quercetin induces the loss of MMP. (A) In brief, 1 × 105 MCF-7 cells were seeded in 24-well plate containing poly-l-lysine-coated coverslip for 24 h and subjected to treatment with 50 µM of quercetin for 6, 12, 24, and 48 h. Then, cells were stained with JC-1 dye and counter stained with DAPI. Images were taken under confocal microscope. Scale bar represents 2 µm. (B) Quantitative analysis of MMP. In brief, 1 × 104 MCF-7 cells were seeded in 96-well plate for 24 h and subjected to treatment with 10, 25, and 50 µM of quercetin for 6–48 h. Change in MMP (Δψm) was quantified by spectroflurometeric analysis using a wavelength of excitation at 505 nm and emission at 527 and 590 nm. The ratio of red fluorescence to green fluorescence was considered as change in MMP. Error bars represent mean ± SEM from three independent experiments. Asterisk indicates the P-value of ≤0.05.

  • N-Benzylcinnamide induces apoptosis in HPV16 and HPV18 cervical cancer cells via suppression of E6 and E7 protein expression

    N‐Benzylcinnamide induces apoptosis in HPV16 and HPV18 cervical cancer cells via suppression of E6 and E7 protein expression

    Administration of N-benzylcinnamide (PT-3) in cervical cancer cells leads to apoptosis in CaSki (HPV-16 positive) cells. A: CaSki (HPV-16 positive) cells were treated with 500 nM PT-3 for 48 H, apoptotic cells were stained using a TUNEL Assay Kit. Nuclear DNA was stained with DAPI; Scale bars, 50 µm; B: Quantitative analysis of apoptotic CaSki (HPV-16 positive) cells (*, P < 0.01, one-way ANOVA).

  • miR-212/132 downregulates SMAD2 expression to suppress the G1/S phase transition of the cell cycle and the epithelial to mesenchymal transition in cervical cancer cells

    miR‐212/132 downregulates SMAD2 expression to suppress the G1/S phase transition of the cell cycle and the epithelial to mesenchymal transition in cervical cancer cells

    MiR-212/132 inhibits cell migration, invasion, and EMT in human cervical cancer cell lines. A and B: MiR-212/132 inhibits the migration and invasion abilities of human cervical cancer cells. Migrated and invasive HeLa (A) and C33A cells (B) were photographed using a microscope, and the number of migrated and invasive cells in every field was counted. C: MiR-212/132 inhibits EMT. Left: Western blotting analysis for the expression of E-cadherin and vimentin in cells transfected with miR-212/132 or the control vector. Right: qRT-PCR analysis for the expression of EMT transcription factors Snail, Slug, and Twist2 and the mesenchymal marker FN1 in HeLa cells transfected with miR-212/132 or the control vector. The control was normalized to 1. *P < 0.05; **P < 0.01; *** P < 0.001. Error bars, SD. D: Immunostaining of E-cadherin (green) and DAPI (blue) after transfection.

  • Interleukin‐1α and brain inflammation
  • Fructosylation generates neo‐epitopes on human serum albumin
  • Alterations of TMEM16a allostery in human retinal microarterioles in long‐standing hypertension
  • Treg‐specific demethylated region activity in isolated regulatory t lymphocytes is a surrogate for disease severity in hepatocellular carcinoma
  • Quercetin protects necrotic insult and promotes apoptosis by attenuating the expression of RAGE and its ligand HMGB1 in human breast adenocarcinoma cells
  • N‐Benzylcinnamide induces apoptosis in HPV16 and HPV18 cervical cancer cells via suppression of E6 and E7 protein expression
  • miR‐212/132 downregulates SMAD2 expression to suppress the G1/S phase transition of the cell cycle and the epithelial to mesenchymal transition in cervical cancer cells

Recently Published Issues

See all

Open Access in IUBMB Life

Click below to read these OnlineOpen Critical Reviews in IUBMB Life for FREE:

Uncovering the roles of miRNAs and their relationship with androgen receptor in prostate cancer
Song ChunJiao, Chen Huan, Xu ChaoYang, Rue GuoMei
Volume 66, Issue 6, June 2014

Mammalian epigenetic mechanisms
Guoqiang Zhang, Sriharsa Pradhan
Volume 66, Issue 4, April 2014

New insights into roles of intermediate filament phosphorylation and progeria pathogenesis
Hidemasa Goto, Masaki Inagaki
Volume 66, Issue 3, March 2014

Understanding circadian gene function: Animal models of tissue-specific circadian disruption
Tana L. Birky, Molly S. Bray
Volume 66, Issue 1, January 2014

Virtual Issue: Molecular Basis of Disease

Understanding Causes and Targeting Therapies

Molecular basis of disease: understanding causes and targeting therapies
Read more

 Molecular basis of disease


News

2014 Impact Factor:
IUBMB Life is pleased to announce its 2014 Impact Factor has increased to 3.143!

2015 Release of Journal Citation Reports®
Source: Thomson Reuters 2014 Citation Data

 Miami Symposium 2016

IUBMB Joint Virtual Issue on Cancer Therapies

BTPR online only


We’re pleased to present a new Joint Virtual Issue on Cancer Therapies in celebration of the IUBMB 2015 Miami Winter Symposium, featuring articles from Biofactors, Biotechnologyand Applied Biochemistry, and IUBMB Life.

2015 IUBMB Life Young Investigator Award

NA

On behalf of the IUBMB, IUBMB Life, and Wiley, it is with great pleasure and honor that we announce Yang Chao as the recipient of the 2015 IUBMB Life - Wiley Young Investigator Award for his article, Aquaporin-4 knockdown ameliorates hypoxic-ischemic cerebral edema in newborn piglets.

Yang Chao, M. D. is an attending physician in Department of Radiology at Dalian Medical University of China, His research interest is in molecular imaging and neuroimaging. The winning article Aquaporin-4 knockdown ameliorates hypoxic-ischemic cerebral edema in newborn piglets is main part of his doctoral thesis under guidance of professor Bian Jie. Yang Chao will be honored with the 2015 IUBMB Life - Wiley Young Investigator Award at the 23rd International Union for Biochemistry and Molecular Biology (IUBMB) Congress and 44th Annual Meeting of the Brazilian Society for Biochemistry and Molecular Biology in Foz do Iguaçu, Brazil from August 24th to 28th, 2015, and his award-winning article will be FREELY available online through the conference.

Please join us in congratulating Mr. Yang Chao as the recipient of the annual IUBMB Life– Wiley Young Investigator Award!

SEARCH

SEARCH BY CITATION