IUBMB Life

Cover image for Vol. 68 Issue 2

Edited By: Angelo Azzi and William J. Whelan

Impact Factor: 3.143

ISI Journal Citation Reports © Ranking: 2014: 102/184 (Cell Biology); 115/290 (Biochemistry & Molecular Biology)

Online ISSN: 1521-6551

Associated Title(s): Biochemistry and Molecular Biology Education, Biotechnology and Applied Biochemistry, BioFactors

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  • Glyoxal induced structural transition of buffalo kidney cystatin to molten globule and aggregates: Anti-fibrillation potency of quinic acid

    Glyoxal induced structural transition of buffalo kidney cystatin to molten globule and aggregates: Anti‐fibrillation potency of quinic acid

    (a) Gel filtration pattern of BKC extracted on Sephacryl S-100HR column. Eluted fractions were monitored by inhibition of caseinolytic activity of papain (▴) and absorbance at 660 nm (•). (b) Electrophoretic pattern of BKC. Lane a contained 60 µg homogenate, lane b contained 60 µg homogenate after alkaline treatment, lane c is 60 µg BKC after Sephacryl S-100 gel filtration. (c) SDS-PAGE of BKC with standard molecular markers. Middle lane contains Phosphorylase b (97.4 kDa), BSA (66 kDa), Ovalbumin (43 kDa), Carbonic Anhydrase (29 kDa), Soyabean trypsin inhibitor (20.1 kDa), and Lysozyme (14.3 kDa; Top to bottom). Lane a contained purified inhibitor and Lane b contained 2 mercaptoethanol treated purified inhibitor.

  • miR-4299 mediates the invasive properties and tumorigenicity of human follicular thyroid carcinoma by targeting ST6GALNAC4

    miR‐4299 mediates the invasive properties and tumorigenicity of human follicular thyroid carcinoma by targeting ST6GALNAC4

    Suppression of ST6GALNAC4 gene inhibits the invasive ability of FTC-238 cells both in vitro and in vivo. A. Suppression of ST6GALNAC4 in FTC-238 cells was analyzed by RNAi approach. B. After shRNA transfection, distinct reduction of ST6GALNAC4 was observed at protein levels by real-time PCR and Western blot analysis. C. The average number of cells that invaded through the filter was assessed by In vitro ECMatrix gel analysis. FTC-238-ST6GALNAC4 shRNA cells were significantly less invasive (*P < 0.05) than the FTC-238 cells and FTC-238-control shRNA cells. D. A decrease of mean tumor weight in mice group with FTC-238-ST6GALNAC4 shRNA tumors was observed, as compared to the control group (*P < 0.05). Data are the means ± SD of triplicate determinants (*P < 0.05).

  • Identification of differentially expressed three novel transcript variants of mouse ARNT gene

    Identification of differentially expressed three novel transcript variants of mouse ARNT gene

    Agarose gel electrophoresis of semi-nested PCR products of Arnt transcript variants in different tissues. RNA isolated from different tissues are shown in gel picture. Band position at 568 bp in all corresponds to the published isoform ArntC1 which served as positive control in our experiments (lane C). PCR products of 996 bp size corresponding to transcript ArntT1 using forward primer ARNE1F (lane T1) was obtained in brain and lung only. PCR product of 772 bp size corresponding to transcript ArntT2 using forward primer ARNE2F (lane T2) was obtained in kidney and liver only. PCR product of 779 bp size corresponding to transcript ArntT3 using forward primer ARNE3F (lane T3) was obtained in kidney and lung only. Reverse primer ARER2 was used in all PCRs. Marker size is shown adjacent to lane M. PCR products of anticipated size are shown by arrow heads.

  • Evidence of pomegranate methanolic extract in antagonizing the endogenous SERM, 27-hydroxycholesterol

    Evidence of pomegranate methanolic extract in antagonizing the endogenous SERM, 27‐hydroxycholesterol

    qRT-PCR. mRNA levels of ERα, pS2 and PR were estimated by real-time PCR after treatments for 24 h. The treatments were PME (100 μg/mL), 27HC (10 μM), PME (100 μg/mL) + 27HC (10μM) and E2 (10 nM). After treatment, cells were harvested, total RNA was isolated and cDNA was prepared for use as a template for gene expression analysis. All values were normalized to the housekeeping gene GAPDH. Data are presented as the fold induction over untreated cells. Data are the mean ± SEM of triplicate amplification reactions from one representative experiment that was repeated with similar results three independent times.

  • Adipose-derived mesenchymal stem cells modulate the immune response in chronic experimental autoimmune encephalomyelitis model

    Adipose‐derived mesenchymal stem cells modulate the immune response in chronic experimental autoimmune encephalomyelitis model

    Photomicrographs of H&E-stained sections of the cerebral cortex (gray and white matter) of rats in all groups showing: A–D ×200 and E–H ×400. The cerebral cortex gray matter in all groups, A: Group I showed normal appearance of gray matter, its layers can be identified. Pia matter (arrow head) and pink-stained neuropil (asterisk) with large pyramidal cells (P), neuroglia cell (g) and blood vessels (arrow) are well observed. B: Group II showed abnormal appearance, its layers are poorly identified. Marked separated pia matter (arrow head), highly vacuolated neuropil (v), many degenerated neurons (n) and also numerous blood vessels (arrow) are also seen. C: Group V nearly reserves its normal appearance. Little separated pia matter (arrow head), normal neuronal cells (P), with some degenerated neurons (n) and also few blood vessels (arrow). D: Group VI slightly reserves its normal appearance, mild separated pia matter (arrow head), still vacuolated neuropil and also numerous degenerated neurons (n). The cerebral cortex white matter in all groups, E: Group I shows longitudinal (Ls) and transverse (Ts) sections of its nerve fibers. Numerous oligodendroglia cells (arrow) arrange in raws between them. F: Group II shows marked degenerated nerve fibers (d) and abnormal unstained myelin with axonal loss (a), few irregularly distributed oligodendrocytes (arrow). G: Group V shows nearly normal appearance of the nerve fibers, central axons surrounded by myelin sheath (a) and also some degenerated fibers (d) are observed. Numerous oligodendroglia (arrow) arranged in rows are seen. H: Group VI shows some degenerated (d) and thickened, irregular nerve fibers (arrow). Few oligodendroglia (arrow head) arranged in rows in between fibers are seen.

  • Modulatory role of 17β-estradiol in the tumor microenvironment of thyroid cancer

    Modulatory role of 17β‐estradiol in the tumor microenvironment of thyroid cancer

    Role of 17β-estradiol in thyroid TME.

  • Glyoxal induced structural transition of buffalo kidney cystatin to molten globule and aggregates: Anti‐fibrillation potency of quinic acid
  • miR‐4299 mediates the invasive properties and tumorigenicity of human follicular thyroid carcinoma by targeting ST6GALNAC4
  • Identification of differentially expressed three novel transcript variants of mouse ARNT gene
  • Evidence of pomegranate methanolic extract in antagonizing the endogenous SERM, 27‐hydroxycholesterol
  • Adipose‐derived mesenchymal stem cells modulate the immune response in chronic experimental autoimmune encephalomyelitis model
  • Modulatory role of 17β‐estradiol in the tumor microenvironment of thyroid cancer

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IUBMB Joint Virtual Issue on Cancer Therapies

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We’re pleased to present a new Joint Virtual Issue on Cancer Therapies in celebration of the IUBMB 2015 Miami Winter Symposium, featuring articles from Biofactors, Biotechnologyand Applied Biochemistry, and IUBMB Life.

2015 IUBMB Life Young Investigator Award

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On behalf of the IUBMB, IUBMB Life, and Wiley, it is with great pleasure and honor that we announce Yang Chao as the recipient of the 2015 IUBMB Life - Wiley Young Investigator Award for his article, Aquaporin-4 knockdown ameliorates hypoxic-ischemic cerebral edema in newborn piglets.

Yang Chao, M. D. is an attending physician in Department of Radiology at Dalian Medical University of China, His research interest is in molecular imaging and neuroimaging. The winning article Aquaporin-4 knockdown ameliorates hypoxic-ischemic cerebral edema in newborn piglets is main part of his doctoral thesis under guidance of professor Bian Jie. Yang Chao will be honored with the 2015 IUBMB Life - Wiley Young Investigator Award at the 23rd International Union for Biochemistry and Molecular Biology (IUBMB) Congress and 44th Annual Meeting of the Brazilian Society for Biochemistry and Molecular Biology in Foz do Iguaçu, Brazil from August 24th to 28th, 2015, and his award-winning article will be FREELY available online through the conference.

Please join us in congratulating Mr. Yang Chao as the recipient of the annual IUBMB Life– Wiley Young Investigator Award!

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