IUBMB Life

Cover image for Vol. 68 Issue 10

Edited By: Angelo Azzi and William J. Whelan

Impact Factor: 2.653

ISI Journal Citation Reports © Ranking: 2015: 108/187 (Cell Biology); 145/289 (Biochemistry & Molecular Biology)

Online ISSN: 1521-6551

Associated Title(s): Biochemistry and Molecular Biology Education, Biotechnology and Applied Biochemistry, BioFactors

Featured

  • Membrane skeleton orchestrates the platelet glycoprotein (GP) Ib-IX complex clustering and signaling

    Membrane skeleton orchestrates the platelet glycoprotein (GP) Ib‐IX complex clustering and signaling

    The membrane GEM domain is not essential for antibody-crosslinking induced GP Ibα clustering. Wild-type GP IbαWT and CT-truncated GP IbαΔPhe555 were untreated or pretreated with MβCD, a known cholesterol depriving and GEMs disrupting reagent, followed by SZ2 staining and antibody crosslinking, as described in Fig. 1. After fixation, the membrane distribution of the GEM domain was revealed by counterstaining the cells with Alexa Fluor® 488 conjugated CT-B. In both cells the GP Ibα clusters (green, b, e, h, and k) co-localized well with the CT-B patches (red, c, f, i, and l), where all GP Ibα clusters associated with the CT-B patches (merged, a, d, g, and j). Larger punctuated GEMs patches were formed in the GP IbαΔPhe555 cells than those in GP IbαWT cells (c and i), suggesting the GEMs forms patches when GP Ibα clusters. Treatment with MβCD did not affect GP Ibα clustering (e and k) but abolished the GEMs (f and l) and the GP Ibα/GEMs-colocalizing structures in both cells (merged, d and j), indicating that the clustering of GP Ibα does not depend on the GP Ibα-associating GEMs, instead, on the presence of the membrane skeletal constraint.

  • Identification of arginine and its “Downstream” molecules as potential markers of breast cancer

    Identification of arginine and its “Downstream” molecules as potential markers of breast cancer

    PCA scores diagrams of BC group versus control group.Abbreviations: Class 1, BC group; Class 2, control group. R2X = 1 and Q2 = 0.99.

  • Upregulation of cyclin-dependent kinase 7 and matrix metalloproteinase-14 expression contribute to metastatic properties of gastric cancer

    Upregulation of cyclin‐dependent kinase 7 and matrix metalloproteinase‐14 expression contribute to metastatic properties of gastric cancer

    Western blotting analysis of proteins in tumor tissues and control.

  • Sequential epitopes of Dermatophagoides farinae allergens identified using peptide microarray-based immunoassay

    Sequential epitopes of Dermatophagoides farinae allergens identified using peptide microarray‐based immunoassay

    Locations of epitopes identified by microarray-based immunoassay on the tertiary structures of mite allergens. (A-1 and A-2), Epitope peptides are marked on the 3D structure of Der f 1 with P1 (46–53aa), P2 (71–78aa), P3 (99–110aa) and P4 (179–186aa). (B-1 and B-2), Epitope peptides are marked on the 3D structure of Der f 2 as P5 (15–22aa), P6 (80–89aa), and P7 (106–113aa). (C-1 and C-2), Epitope peptides are marked on the 3D structure of Der f 4 as P8 (69–82aa), P9 (107–116aa), P10 (225–232aa), P11 (261–268aa), P12 (355–365aa), and P13 (483–496aa). (D) Epitope peptides are marked on the 3D structure of Der f 5 as P14 (102–109aa). (E) Epitope peptides are marked on the 3D structure of Der f 7 as P15 (32–39aa), P16 (52–64aa), and P17 (100–107aa).

  • Molecular basis of antibody-mediated neutralization and protection against flavivirus

    Molecular basis of antibody‐mediated neutralization and protection against flavivirus

    Epitopes bound by flavivirus neutralizing MAbs. E protein is shown as a cartoon/surface and colored red (domain I), yellow (domain II), and blue (domain III). Fragment antigen-binding (Fab) is shown as a cartoon and colored in green. The Fab-E interactions are calculated by the CCP4 suite of programs with a maximal cutoff distance of 4.6 Å for Van der Waals contacts. The Fab footprint is colored in green on the E protein surface, and denoted by dashed circles. A: 2A10G6 binds to the DII fusion loop epitope of ZIKV E (upper) (PDB:5JHL); the footprint of 2A10G6 (the bottom). B: 5H2 binds to the DI epitope of DENV4 E (upper) (PDB: 3UAJ); the footprint of 5H2 (bottom). C: E16 binds to the DIII epitope of WNV E by docking the E16-DIII complex onto the WNV E structure (upper) (PDB: 1ZTX; 2HG0); the footprint of 5H2 (botton). D: B7 binds to the epitope in the E-dimer of DENV2 (upper) (PDB: 4UT6); the footprint of B7 (bottom). E: CR4354 binds to the epitope across from three E monomers in neighboring E-dimers of WNV (upper) (PDB: 3IYW); the footprint of CR4354 (botton).

  • Membrane skeleton orchestrates the platelet glycoprotein (GP) Ib‐IX complex clustering and signaling
  • Identification of arginine and its “Downstream” molecules as potential markers of breast cancer
  • Upregulation of cyclin‐dependent kinase 7 and matrix metalloproteinase‐14 expression contribute to metastatic properties of gastric cancer
  • Sequential epitopes of Dermatophagoides farinae allergens identified using peptide microarray‐based immunoassay
  • Molecular basis of antibody‐mediated neutralization and protection against flavivirus

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IUBMB Joint Virtual Issue on Cancer Therapies

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We’re pleased to present a new Joint Virtual Issue on Cancer Therapies in celebration of the IUBMB 2015 Miami Winter Symposium, featuring articles from Biofactors, Biotechnologyand Applied Biochemistry, and IUBMB Life.

2015 IUBMB Life Young Investigator Award

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On behalf of the IUBMB, IUBMB Life, and Wiley, it is with great pleasure and honor that we announce Yang Chao as the recipient of the 2015 IUBMB Life - Wiley Young Investigator Award for his article, Aquaporin-4 knockdown ameliorates hypoxic-ischemic cerebral edema in newborn piglets.

Yang Chao, M. D. is an attending physician in Department of Radiology at Dalian Medical University of China, His research interest is in molecular imaging and neuroimaging. The winning article Aquaporin-4 knockdown ameliorates hypoxic-ischemic cerebral edema in newborn piglets is main part of his doctoral thesis under guidance of professor Bian Jie. Yang Chao will be honored with the 2015 IUBMB Life - Wiley Young Investigator Award at the 23rd International Union for Biochemistry and Molecular Biology (IUBMB) Congress and 44th Annual Meeting of the Brazilian Society for Biochemistry and Molecular Biology in Foz do Iguaçu, Brazil from August 24th to 28th, 2015, and his award-winning article will be FREELY available online through the conference.

Please join us in congratulating Mr. Yang Chao as the recipient of the annual IUBMB Life– Wiley Young Investigator Award!

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