IUBMB Life

Cover image for Vol. 67 Issue 3

Edited By: Angelo Azzi and William J. Whelan

Impact Factor: 2.755

ISI Journal Citation Reports © Ranking: 2013: 115/185 (Cell Biology); 154/291 (Biochemistry & Molecular Biology)

Online ISSN: 1521-6551

Associated Title(s): Biochemistry and Molecular Biology Education, Biotechnology and Applied Biochemistry, BioFactors

Featured

  • Cellular and molecular mechanisms of inflammation-induced angiogenesis

    Cellular and molecular mechanisms of inflammation‐induced angiogenesis

    Role of microRNAs and HO-1 in monocyte–macrophage differentiation. Monocytes can differentiate into two distinct macrophage subsets: proinflammatory and antiangiogenic M1 or anti-inflammatory and proangiogenic M2/TAM. Macrophage polarization is controlled by several cytokines, growth factors, and microRNAs. HO-1 promotes macrophage differentiation into M2 phenotype.

  • Modulation of protein synthesis by polyamines

    Modulation of protein synthesis by polyamines

    Effect of spermidine on Qβ RNA-directed protein synthesis. Qβ RNA-directed protein synthesis was performed by changing Mg2+ concentrations in an E. coli cell-free system. Three kinds of proteins synthesized were separated by SDS-polyacrylamide gel electrophoresis. A. Total proteins. B. RNA replicase. C. Coat protein. D. A2 protein. Black circle, no SPD; red circle, +1 mM SPD; blue circle, +2 mM SPD.

  • c-Fos is involved in inhibition of human bladder carcinoma T24 cells by Brazilin

    c‐Fos is involved in inhibition of human bladder carcinoma T24 cells by Brazilin

    c-Fos expression. (A) c-Fos mRNA level by qPCR, presented in fold of change. (B) Western blot of c-Fos at 32 µg/mL brazilin for 6 h. (C) Tumor cell specific upregulation by qPCR. (D) Brazilin effect on different tumor cells.

  • Aquaporin-4 knockdown ameliorates hypoxic-ischemic cerebral edema in newborn piglets

    Aquaporin‐4 knockdown ameliorates hypoxic‐ischemic cerebral edema in newborn piglets

    Apparent diffusion coefficient (ADC) imaging and values in the hypoxia–ischemia piglet model. Magnetic resonance imaging scanning was performed in all groups of piglets pretreated with or without AQP4 siRNA or scrambled siRNA before (0 h) and after ischemia–hypoxia exposure at various time points (1, 3, 6, 9, and 12 h). (A) Representative ADC images show the location, extent, and image timing of ischemic damage in hypoxic–ischemic piglets pretreated with scrambled siRNA. (B) Changes of ADC values at various time points in group A. (C) Changes of ADC values at various time points in group B.

  • MicroRNA-494 sensitizes colon cancer cells to fluorouracil through regulation of DPYD

    MicroRNA‐494 sensitizes colon cancer cells to fluorouracil through regulation of DPYD

    miR-494 promoted apoptosis of colon cancer cells with presence of 5-Fu. (A) SW480 cells were treated with 0 or 0.5 μg/mL of 5-Fu, and cell apoptosis was detected by TUNEL assay. (B) SW480/5-Fu cells were treated with 0 or 5 μg/mL of 5-Fu, and cell apoptosis was also detected. (C) The pro- and cleaved caspase-3 protein level in the above cells was detected by Western blot assay. **P < 0.01.

  • MRTF-A and STAT3 promote MDA-MB-231 cell migration via hypermethylating BRSM1

    MRTF‐A and STAT3 promote MDA‐MB‐231 cell migration via hypermethylating BRSM1

    BRMS1 inhibited MDA-MB-231 cell migration. A. The effect of overexpressed BRMS1 on MDA-MB-231 migration for 48 h was detected by the wound-healing assay. Bars correspond to mean ± SD. **P < 0.01 as compared with pcDNA3.1. Scale bar corresponds to 100 μm. Student's t-test was used for the comparison of two independent groups. B. The transwell assay was used to detect the inhibitory effects on migration of BRMS1. The number of cells migrated to the lower side of the transwell chambers was counted and photographed in five fields (the upper, the lower, the left, the right, and the middle) of three independent experiments, and the fold migration was calculated by SPSS statistical software (**P < 0.01.) C. After MDA-MB-231 cells were transfected with BRMS1 for 24 h, total RNA was isolated and the expression of uPA and OPN were examined by qPCR. GAPDH was used as a loading control. **P < 0.01 as compared with pcDNA3.1. Student's t-test was used for the comparison of two independent groups. D. At 24 h post-transfection with BRMS1, total proteins were extracted and the protein levels of uPA and OPN were detected by western blot analysis. β-actin was used as a loading control. The bar graph depicts the relative protein expression (fold) based on densitometric analysis of the Western blotting data. Bars correspond to mean ± SD. **P < 0.01 as compared with pcDNA3.1. Student's t-test was used for the comparison of two independent groups.

  • Dose-dependent effect of 2-deoxy-D-glucose on glycoprotein mannosylation in cancer cells

    Dose‐dependent effect of 2‐deoxy‐D‐glucose on glycoprotein mannosylation in cancer cells

    Visualization of mannose-containing glycoprotein spectrum on 2DG treatment. Autoradiography film of HCT116 cells incubated with radioactively labeled d-[1–14C]mannose on treatment with 2DG (1 or 4 mM) or without 2DG. Increased mannose content was observed over the entire spectrum of glycoproteins and was most pronounced on treatment with 1 mM 2DG. SeeBlue Plus 2 Prestained Standard (Life Technologies, Darmstadt, Germany) was used as a molecular weight marker.

  • Cellular and molecular mechanisms of inflammation‐induced angiogenesis
  • Modulation of protein synthesis by polyamines
  • c‐Fos is involved in inhibition of human bladder carcinoma T24 cells by Brazilin
  • Aquaporin‐4 knockdown ameliorates hypoxic‐ischemic cerebral edema in newborn piglets
  • MicroRNA‐494 sensitizes colon cancer cells to fluorouracil through regulation of DPYD
  • MRTF‐A and STAT3 promote MDA‐MB‐231 cell migration via hypermethylating BRSM1
  • Dose‐dependent effect of 2‐deoxy‐D‐glucose on glycoprotein mannosylation in cancer cells

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Click below to read these OnlineOpen Critical Reviews in IUBMB Life for FREE:

Uncovering the roles of miRNAs and their relationship with androgen receptor in prostate cancer
Song ChunJiao, Chen Huan, Xu ChaoYang, Rue GuoMei
Volume 66, Issue 6, June 2014

Mammalian epigenetic mechanisms
Guoqiang Zhang, Sriharsa Pradhan
Volume 66, Issue 4, April 2014

New insights into roles of intermediate filament phosphorylation and progeria pathogenesis
Hidemasa Goto, Masaki Inagaki
Volume 66, Issue 3, March 2014

Understanding circadian gene function: Animal models of tissue-specific circadian disruption
Tana L. Birky, Molly S. Bray
Volume 66, Issue 1, January 2014

Virtual Issue: Molecular Basis of Disease

Understanding Causes and Targeting Therapies

Molecular basis of disease: understanding causes and targeting therapies
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 Molecular basis of disease


IUBMB Life moving to online only in 2015

 IUB Life online only

In 2015, IUBMB Life will move to online only publication, in trend with Wiley's path of digital transformation, as the company invests in the customizable, technology-enabled products and services needed by our customers in the future.

The gradual migration of journals from print to solely online is part of this transition, supporting investment in forward-looking innovation and building online communities for journal readers and our society partners.

IUBMB Joint Virtual Issue on Cancer Therapies

BTPR online only


We’re pleased to present a new Joint Virtual Issue on Cancer Therapies in celebration of the IUBMB 2015 Miami Winter Symposium, featuring articles from Biofactors, Biotechnologyand Applied Biochemistry, and IUBMB Life.

2014 IUBMB Life Young Investigator Award

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On behalf of the IUBMB, IUBMB Life, and Wiley, it is with great pleasure and honor that we announce Liudmila Abrosimova as the recipient of the 2014 IUBMB Life - Wiley Young Investigator Award for her article, Thermo-switchable activity of the restriction endonuclease Ssoll achieved by site-directed enzyme modification.

Ms. Abrosimova is affiliated with the Department of Bioengineering and Bioinformatics, Department of Chemistry, and Belozersky Institute of Physico-Chemical Biology at Lomonosov Moscow State University. She will be honored with the 2014 IUBMB Life - Wiley Young Investigator Award at the 15th IUBMB - 24th FAOBMB - TSBMB Conference this October 21 - 26, in Taipei, Taiwan, and her award-winning article will be FREELY available online through the conference.

Please join us in congratulating Ms. Abrosimova as the recipient of the annual IUBMB Life – Wiley Young Investigator Award!

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