Cover image for Vol. 38 Issue 2

Editor-in-Chief: Ziad El Rassi

Impact Factor: 2.482

ISI Journal Citation Reports © Ranking: 2015: 26/75 (Chemistry Analytical); 38/77 (BIOCHEMICAL RESEARCH METHODS)

Online ISSN: 1522-2683

Associated Title(s): Journal of Separation Science



“Glycosylation is one of the most important PTMs of proteins, playing crucial roles in biological processes such as cell recognition, cell–cell interaction, signaling, embryonic development and recognition of hormones and toxins. It is estimated that ~50% of all proteins in mammalian cells are glycosylated at any given time. As abnormal protein glycosylation has been correlated with several diseases such as cancer, inflammatory diseases, congenital disorders, etc., glycosylated proteins have found utility as valuable targets for developing novel vaccines, and as useful biomarkers for early detection and diagnosis of a disease. As a result, the study of the glycoproteome has received particular attention in the recent past”, as highlighted in Issue 24 2011, a Focus Issue on Glycomics and Glycoproteomics.

To follow on from this, we present here a Virtual Issue compiled of the 20 most-downloaded articles in the field (published over the last two years).


Iulia M. Lazar, Alexandru C. Lazar, Diego F. Cortes, Jarod L. Kabulski
Recent advances in the MS analysis of glycoproteins: Theoretical considerations

Diego F. Cortes, Jarod L. Kabulski, Alexandru C. Lazar, Iulia M. Lazar
Recent advances in the MS analysis of glycoproteins: Capillary and microfluidic workflows

Jun Hirabayashi, Atsushi Kuno, Hiroaki Tateno
Lectin-based structural glycomics: A practical approach to complex glycans

Jingjing You, Anna Fitzgerald, Paul J. Cozzi, Zhenjun Zhao, Peter Graham, Pamela J. Russell, Bradley J. Walsh, Mark Willcox, Ling Zhong, Valerie Wasinger, Yong Li
Post-translation modification of proteins in tears

Marijana Rucevic, Douglas Hixson, Djuro Josic
Mammalian plasma membrane proteins as potential biomarkers and drug targets

Jeremy Primack, Gregory C. Flynn, Hai Pan
A high-throughput microchip-based glycan screening assay for antibody cell culture samples

Zoltan Szabo, András Guttman, Tomas Rejtar, Barry L. Karger
Improved sample preparation method for glycan analysis of glycoproteins by CE-LIF and CE-MS

Sílvia Barrabés, Ariadna Sarrats, Esther Fort, Rafael De Llorens, Pauline M. Rudd, Rosa Peracaula
Effect of sialic acid content on glycoprotein pI analyzed by two-dimensional electrophoresis

Eugen Sisu, Corina Flangea, Alina Serb, Andreas Rizzi, Alina D. Zamfir
High-performance separation techniques hyphenated to mass spectrometry for ganglioside analysis

Chen Li, Eugene Zolotarevsky, Ian Thompson, Michelle A. Anderson, Diane M. Simeone, John M. Casper, Michael C. Mullenix, David M. Lubman
A multiplexed bead assay for profiling glycosylation patterns on serum protein biomarkers of pancreatic cancer

Roland Müller, Martina Marchetti-Deschmann, Helmut Elgass, Heimo Breiteneder, Martin Kratzmeier, Günter Allmaier
Molecular weight determination of high molecular mass (glyco)proteins using CGE-on-a-chip, planar SDS-PAGE and MALDI-TOF-MS

E. Jane Maxwell, Chitra Ratnayake, Roxana Jayo, Xuefei Zhong, David D. Y. Chen
A promising capillary electrophoresis–electrospray ionization–mass spectrometry method for carbohydrate analysis

Petr Smejkal, Ákos Szekrényes, Markéta Ryvolová, František Foret, András Guttman, Fritz Bek, Mirek Macka
Chip-based CE for rapid separation of 8-aminopyrene-1,3,6-trisulfonic acid (APTS) derivatized glycans

Andrea Robotti, Massimo Natale, Alessandra Giuliano Albo, Katarzyna Lis, Simona Perga, Fabiana Marnetto, Francesca Gilli, Antonio Bertolotto
Acute-phase proteins investigation based on lectins affinity capture prior to 2-DE separation: Application to serum from multiple sclerosis patients

Lijun Zhang, Xiaofang Jia, Xiaojun Zhang, Jing Cao, Pengyuan Yang, Chao Qiu, Yinzhong Shen, Fang Ma, Li Liu, Jianjun Sun, Fang Shen, Lin Yin, Liwen Liu, Yamin Yao, Hongzhou Lu
Alpha-1 antitrypsin variants in plasma from HIV-infected patients revealed by proteomic and glycoproteomic analysis

Alina D. Zamfir, Corina Flangea, Eugen Sisu, Daniela G. Seidler, Jasna Peter-Katalinic
Combining size-exclusion chromatography and fully automated chip-based nanoelectrospray quadrupole time-of-flight tandem mass spectrometry for structural analysis of chondroitin/dermatan sulfate in human decorin

Kongsak Boonyapranai, Hsien-Yu Tsai, Miles Chih-Ming Chen, Supawadee Sriyam, Supachok Sinchaikul, Suree Phutrakul, Shui-Tien Chen
Glycoproteomic analysis and molecular modeling of haptoglobin multimers

Maggie P. Y. Lam, Edward Lau, S. O. Siu, Dominic C. M. Ng, Ricky P. W. Kong, Philip C. N. Chiu, William S. B. Yeung, Clive Lo, Ivan K. Chu
Online combination of reversed-phase/reversed-phase and porous graphitic carbon liquid chromatography for multicomponent separation of proteomics and glycoproteomics samples

Yu-Ki Matsuno, Weijie Dong, Seiya Yokoyama, Suguru Yonezawa, Takuro Saito, Mitsukazu Gotoh, Hisashi Narimatsu, Akihiko Kameyama
Improved method for immunostaining of mucin separated by supported molecular matrix electrophoresis by optimizing the matrix composition and fixation procedure

Ramarao Seriramalu, Wei Wei Pang, Jaime Jacqueline Jayapalan, Emida Mohamed, Puteri Shafinaz Abdul-Rahman, Anita Zarina Bustam, Alan Soo-Beng Khoo, Onn Haji Hashim
Application of champedak mannose-binding lectin in the glycoproteomic profiling of serum samples unmasks reduced expression of alpha-2 macroglobulin and complement factor B in patients with nasopharyngeal carcinoma