STEM CELLS

Cover image for Vol. 34 Issue 12

Edited By: Jan A. Nolta

Impact Factor: 5.902

ISI Journal Citation Reports © Ranking: 2015: 3/21 (CELL & TISSUE ENGINEERING); 8/70 (Hematology); 14/161 (Biotechnology & Applied Microbiology); 24/213 (Oncology); 34/187 (Cell Biology)

Online ISSN: 1549-4918

Associated Title(s): STEM CELLS Translational Medicine

Featured

  • Wnt Signaling Regulates Airway Epithelial Stem Cells in Adult Murine Submucosal Glands

    Wnt Signaling Regulates Airway Epithelial Stem Cells in Adult Murine Submucosal Glands

    SMG-derived cells are capable of generating glandular and surface epithelium in denuded rat tracheal xenografts. Denuded rat tracheal xenografts were seeded with epithelial cell mixtures derived from tdTomato-expressing cells isolated from tracheal SMGs and combined with nontransgenic cells isolated from the SAE at a 1:10 ratio, respectively. (A): tdTomato-marked SMG-derived cells were capable of generating both surface airway epithelial clones (yellow arrowheads) and gland-like epithelial-derived structures (white arrowheads). (B): SMG-derived progenitors gave rise to a pseudostratified columnar epithelium that resembled normal tracheal SAE by Integrin β4 (Intβ4) expression and the presence of ciliated cells seen by phase contrast (Bi, indicated by yellow arrowheads). (C-D): tdTomato-marked SMG-derived cells also generated acetylated α-tubulin (αTub), positive cilia (C), and a CK5 expressing basal cell layer (D). Scale bars represent 100 μm in lower magnification panels and 10 μm in higher magnification panels. Abbreviations: CK5, cytokeratin 5, SAE, surface airway epithelium; SMG, submucosal glands.

  • Inhibition of lncRNA MIR31HG Promotes Osteogenic Differentiation of Human Adipose-Derived Stem Cells

    Inhibition of lncRNA MIR31HG Promotes Osteogenic Differentiation of Human Adipose‐Derived Stem Cells

    MIR31HG knockdown promoted heterotopic bone formation in vivo. (A) Schematic diagram illustrating the experimental setup. (B) H&E staining, Masson's trichrome staining, and immunohistochemical staining of OCN in shNC, shMIR31HG−1, shMIR31HG−2, NC, and MIR31HG groups. Scale bar: 50 μm. Abbreviations: OCN, osteocalcin; shNC, scramble control.

  • Same-Single-Cell Analysis of Pacemaker-Specific Markers in Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Subtypes Classified by Electrophysiology

    Same‐Single‐Cell Analysis of Pacemaker‐Specific Markers in Human Induced Pluripotent Stem Cell‐Derived Cardiomyocyte Subtypes Classified by Electrophysiology

    HCN4, Isl1 staining and action potential profile acquired from the same human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes. A representative image showing HCN4 and Isl1 staining and action potential profile for each hiPSC-derived cardiomyocyte for direct comparisons enabling analysis of gene expression by cardiomyocyte subtypes. Abbreviations: HCN4, hyperpolarization-activated cyclic nucleotide-modulated channel 4; Isl1, Islet 1.

  • CREG1 Interacts with Sec8 to Promote Cardiomyogenic Differentiation and Cell-Cell Adhesion

    CREG1 Interacts with Sec8 to Promote Cardiomyogenic Differentiation and Cell‐Cell Adhesion

    Chimeric analysis. (A): A diagram depicts chimeric EB analysis. (B): WT ES cells stably transfected with GFP under the control of the αMHC were mixed in 1:1 ration with Creg1−/− ES cells transfected with αMHC-mCherry and cultured in hanging drops for 1 day followed by suspension culture for 4 days. The chimeric EBs were then cultured on glass coverslips for 7 days and immunostained for GFP and mCherry. Arrows indicate nuclear accumulation of mCherry in Creg1−/− cardiomyocytes. (C): GFP-positive wild-type and mCherry-positive Creg1−/− myocytes in beating EBs were counted and plotted as a percentage of total fluorescent cells (n = 7, p < .01). (D): 5 + 7 day Creg1−/−-αMHC-mCherry [LEFT RIGHT ARROW] unlabeled wild-type (1:1 mixing) chimeric EBs were immunostained for cardiac myosin heavy chain and mCherry. Arrows indicate mCherry-positive Creg1−/− cardiomyocytes. Abbreviations: EB, embryoid body; GFP, green fluorescence protein; αMHC, α-myosin heavy chain promoter; WT, wild-type.

  • Anterior–Posterior Patterning of Definitive Endoderm Generated from Human Embryonic Stem Cells Depends on the Differential Signaling of Retinoic Acid, Wnt-, and BMP-Signaling

    Anterior–Posterior Patterning of Definitive Endoderm Generated from Human Embryonic Stem Cells Depends on the Differential Signaling of Retinoic Acid, Wnt‐, and BMP‐Signaling

    Midgut/hindgut specification by Wnt/beta-catenin-signaling. (A): Relative gene expression of the midgut/hindgut marker genes CDX2, HOXC5, HOXC6, and HOXB8. The effect of the removal of individual factors from the medium is shown as indicated in the legend to each figure. Values are relative quantities (CNRQ) after normalization to three stably expressed housekeeping genes and are shown as means ± SEM, n = 4–10. ***, p < .001; **, p < .01; *, p < .05, ANOVA plus Dunnett's post test. (B): Immunofluorescence analysis of CDX2/FOXA2 coexpression in midgut/hindgut-like cells. SOX2 protein expression was rarely detected. The merge of the red and green channel is shown in yellow. The merge of the red and blue channel is shown in purple and shows cells that express CDX2 independent of endoderm progeny (marked by the dotted line). As a consequence of the CHIR-99021 removal from the culture medium immunofluorescence staining for CDX2 completely disappeared. Original magnification ×10. Scale bar = 100 µM. Abbreviations: ATRA, all-trans-retinoic acid.

  • Regulation of WNT Signaling by VSX2 During Optic Vesicle Patterning in Human Induced Pluripotent Stem Cells

    Regulation of WNT Signaling by VSX2 During Optic Vesicle Patterning in Human Induced Pluripotent Stem Cells

    βCATENIN localization in d14 VSX2WT and VSX2R200Q hiPSC-OVs. D14 VSX2WT (A–H) or VSX2R200Q (I–P) hiPSC-OVs were immunolabeled with βCATENIN (green), VSX2 (red), and MITF (blue) primary antibodies. Examples of βCATENIN nuclear localization are designated by white arrows (E–H). Panels E–H and M–P are cropped magnifications of the outlined areas in panels A–D and I–L, respectively. Also see Fig. S3 for identical images with βCATENIN and DAPI-labeled nuclei. (Q) Graph of percent of βCATENIN nuclear localization in VSX2+ nuclei for WT and R200Q d14 hiPSC-OVs. **p = .01. Scale bars = 50 µm.

  • Wnt Signaling Regulates Airway Epithelial Stem Cells in Adult Murine Submucosal Glands
  • Inhibition of lncRNA MIR31HG Promotes Osteogenic Differentiation of Human Adipose‐Derived Stem Cells
  • Same‐Single‐Cell Analysis of Pacemaker‐Specific Markers in Human Induced Pluripotent Stem Cell‐Derived Cardiomyocyte Subtypes Classified by Electrophysiology
  • CREG1 Interacts with Sec8 to Promote Cardiomyogenic Differentiation and Cell‐Cell Adhesion
  • Anterior–Posterior Patterning of Definitive Endoderm Generated from Human Embryonic Stem Cells Depends on the Differential Signaling of Retinoic Acid, Wnt‐, and BMP‐Signaling
  • Regulation of WNT Signaling by VSX2 During Optic Vesicle Patterning in Human Induced Pluripotent Stem Cells

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STEM CELLS Video Highlight

Video abstract from Drs. Cox, Hetz, Liao, Aertker, Ewing-Cobbs, Juranek, Savitz, Jackson, Romanowska-Pawliczek, Triolo, Dash, Pedroza, Lee, Worth, Aisiku, Choi, Holcomb, and Kitagawa on their recently published STEM CELLS paper entitled, "Treatment of Severe Adult Traumatic Brain Injury Using Bone Marrow Mononuclear Cells" Read the Paper Here

Video abstract from Drs. Barnawi, Al-Khaldi, Sleiman, Sarkar, Al-Dhfyan, Al-Mohanna, Ghebeh, and Al-Alwan on their recently published STEM CELLS paper entitled, "Fascin is Critical for the Maintenance of Breast Cancer Stem Cell Pool Predominantly via the Activation of the Notch Self-Renewal Pathway" Read the Paper Here

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