STEM CELLS

Cover image for Vol. 34 Issue 1

Edited By: Jan A. Nolta

Impact Factor: 6.523

ISI Journal Citation Reports © Ranking: 2014: 2/21 (CELL & TISSUE ENGINEERING); 4/68 (Hematology); 10/163 (Biotechnology & Applied Microbiology); 20/211 (Oncology); 33/184 (Cell Biology)

Online ISSN: 1549-4918

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  • MiR-375 is Essential for Human Spinal Motor Neuron Development and May Be Involved in Motor Neuron Degeneration

    MiR‐375 is Essential for Human Spinal Motor Neuron Development and May Be Involved in Motor Neuron Degeneration

    MiR-375 is activated upon REST KO. (A): Experimental schematic to identify Rest regulated miRNAs. Rest−/− and Rest+/+ mouse ESC were differentiated into NSC using a small molecule inhibitor (SB431542) of the TGFβ pathway. Total RNA was extracted at both ESC and NSC stages, small RNA isolated and sequenced by SOLiD sequencing. (B): Heatmap displaying pair-wise differential regulation of several hundred miRNAs in response to neural induction as well as Rest KO. (C): Schema to identify direct Rest targets. (D): miR-375 is activated upon Rest KO as well as during neural induction of ESC (highlighted in red). Abbreviations: KO, knockout; mESC, mouse embryonic stem cell; miRNA, microRNA; NSC, neural stem cell.

  • Induced Pluripotent Stem Cell-Derived Natural Killer Cells for Treatment of Ovarian Cancer

    Induced Pluripotent Stem Cell‐Derived Natural Killer Cells for Treatment of Ovarian Cancer

    PB-NK cells and iPSC-derived NK cells mediate in vivo killing of ovarian cancer cells in a MA-148 tumor model. Mice were inoculated with 2 × 105 MA-148 GFP:Luc tumor cells 4 days prior to NK cell injection. Bioluminescent imaging was used to monitor tumor establishment and growth. Day-1 images were used to group mice prior to NK cell injection. (A): Bioluminescent imaging of mice treated with overnight PB-NK, aAPC PB-NK, or iPS-NK cells as indicated in figures. (B): Dot plots representing each group at shown time points. Bars are plotted at the mean. (C): Means + SEM plotted for each group as shown. All treatment groups were statistically significantly different over the treatment period (p < .0001) compared to tumor only, and iPSC-NK were significantly better than aAPC PB-NK (p = .014). Abbreviations: aAPC, artificial antigen presenting cells; aAPC PB-NK, aAPC expanded PB-NK cells; iPSC-NK, induced pluripotent stem cell-derived NK cells; PB-NK, peripheral blood-derived NK cells.

  • P-Selectin Sustains Extramedullary Hematopoiesis in the Gata1low Model of Myelofibrosis

    P‐Selectin Sustains Extramedullary Hematopoiesis in the Gata1low Model of Myelofibrosis

    Deletion of P-selectin does not rescue the maturation defects of Gata1low mice but prevents their emperipolesis with neutrophils and TGF-β accumulation. (A): Hematoxylin-eosin staining of representative spleen sections from WT, P-selnull, Gata1low, and P-selnullGata1low mice, as indicated. MK are indicated by yellow arrows. Original magnification ×20. A representative scale bar (50 μm) is indicated in the first top panel on the left. (B): Representative transmission electron microscopy (TEM) (left panels) and immune-TEM for TGF-β1 (middle and right panels) of MK from spleen sections of Gata1low littermates containing (top panels) or not (bottom panels) P-selectin. Representative TGF-β-gold-particles are indicated by yellow arrows. The numbers below the panels indicate mean (±SD) frequency of emperipolesis events per square millimeter of spleen section and mean (±SD) content of TGF-β-gold-particles per 14 μm2 of MK cytoplasm. In old mice, approximately 50% of Gata1low MK are engaged in emperipolesis almost exclusively with neutrophils while neutrophils were seldomly observed with MK from P-selnull/Gata1low mice. Magnification ×4,400 in the quadrants and ×30,000 in the TGF-β-immuno-gold quadrant. Representative scale bars (1 μm) are indicated in the first and last panel on the top. Additional TEM images of MK from Gata1low and P-selnullGata1low mice are presented at lower magnification in Supporting Information Figure S4. Abbreviations: MK, megakaryocytes; WT, wild type.

  • LncRNA-Hh Strengthen Cancer Stem Cells Generation in Twist-Positive Breast Cancer via Activation of Hedgehog Signaling Pathway

    LncRNA‐Hh Strengthen Cancer Stem Cells Generation in Twist‐Positive Breast Cancer via Activation of Hedgehog Signaling Pathway

    lncRNA-Hh promotes breast cancer tumorigenesis in nude mice. (A): Photos of the tumor-bearing BALB/c female nude mice and the xenograft tumors dissected from the nude mice at 5 weeks after being subcutaneously injected with MCF-7/lncRNA-Hh, MDA-MB-231/shRNA-lncRNA-Hh mammosphere cells and their control mammosphere cells. (B): Tumor growth curve. The data were generated from three mice in four different groups described in (A). Data are the means ± SD of three independent experiments performed in triplicate (*, p ≤ .05; **, p ≤ .01). (C): Quantitative real-time polymerase chain reaction was used to test levels of lncRNA-Hh in MDA-MB-231-sh/lncRNA-Hh tumor tissue and MCF-7/lncRNA-Hh tumor tissue and their control tumor tissues. (D): Immunoblotting analysis of GAS1, GLI1, SOX2, and OCT4 in the tumor tissues described in (C). β-Actin was used as a loading control. Data are the means ± SD of three independent experiments in (C) and (D). (E): Representative photomicrographs of tumor tissue sections stained with SOX2-specific polyclonal antibody (magnification of 400×). Scale bars = 100 μm. Abbreviations: GAS1, growth arrest-specific 1; lncRNA-Hh, hedgehog pathway associated long noncoding RNA.

  • Concise Review: Pluripotent Stem Cell-Derived Cardiac Cells, A Promising Cell Source for Therapy of Heart Failure: Where Do We Stand?

    Concise Review: Pluripotent Stem Cell‐Derived Cardiac Cells, A Promising Cell Source for Therapy of Heart Failure: Where Do We Stand?

    Step by step differentiation of HUES cells into cardiomyocytes. HUES cells are treated with Wnt and BMP2 in the presence of SU5402, a FGFR/VEGFR inhibitor to drive them toward a mesendodermal cell fate (monitored by EOMES and MESP1 expression). Then cells are treated with a Wnt inhibitor, SHH to reach the state of cardiac progenitor cell (NKX2.5+ cells) and sorted with anti-SSEA1 antibody. Cells plated on fibronectin were treated with FGF2 to drive them toward actinin+ cardiomyocytes. Abbreviations: HUES cells, human embryonic stem cells; SHH, Sonic Hedgehog.

  • Concise Review: Fluorescent Reporters in Human Pluripotent Stem Cells: Contributions to Cardiac Differentiation and Their Applications in Cardiac Disease and Toxicity

    Concise Review: Fluorescent Reporters in Human Pluripotent Stem Cells: Contributions to Cardiac Differentiation and Their Applications in Cardiac Disease and Toxicity

    Sarcomeric organization in hiPS-derived cardiomyocytes improves upon maturation. Left: 30-day-old hiPSC-derived CMs cultured in standard CM medium. Right: hiPSC-derived CMs after culture in commercial media optimized for cardiomyocyte maturation. Scale bar = 15 μM . Abbreviations: CM, cardiomyocyte; hiPS, human induced pluripotent stem.

  • MiR‐375 is Essential for Human Spinal Motor Neuron Development and May Be Involved in Motor Neuron Degeneration
  • Induced Pluripotent Stem Cell‐Derived Natural Killer Cells for Treatment of Ovarian Cancer
  • P‐Selectin Sustains Extramedullary Hematopoiesis in the Gata1low Model of Myelofibrosis
  • LncRNA‐Hh Strengthen Cancer Stem Cells Generation in Twist‐Positive Breast Cancer via Activation of Hedgehog Signaling Pathway
  • Concise Review: Pluripotent Stem Cell‐Derived Cardiac Cells, A Promising Cell Source for Therapy of Heart Failure: Where Do We Stand?
  • Concise Review: Fluorescent Reporters in Human Pluripotent Stem Cells: Contributions to Cardiac Differentiation and Their Applications in Cardiac Disease and Toxicity

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STEM CELLS Video Highlight

Video abstract from Drs. Evans and Janeczek’s on their recently published STEM CELLS paper entitled, "Transient Canonical Wnt Stimulation Enriches Human Bone Marrow Mononuclear Cell Isolates for Osteoprogenitors" Read the Paper here

Video abstract from Drs. Ambrosio and Barchowsky on their recently published STEM CELLS paper entitled, "Arsenic Promotes NF-κB-Mediated Fibroblast Dysfunction and Matrix Remodeling to Impair Muscle Stem Cell Function" Read the Paper here

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