Insensitivity of Human iPS Cells-derived Mesenchymal Stem Cells to Interferon-γ -induced HLA Expression Potentiates Repair Efficiency of Hind Limb Ischemia in Immune Humanized NOD Scid Gamma Mice
Yue-Qi Sun, Yuelin Zhang, Xin Li, Meng-Xia Deng, Wen-Xiang Gao, Yin Yao, Sin-Ming Chiu, Xiaoting Liang, Fei Gao, Camie W. Chan, Hung-Fat Tse, Jianbo Shi, Qing-Ling Fu and Qizhou Lian
Accepted manuscript online: 14 JUL 2015 10:52AM EST | DOI: 10.1002/stem.2094
Low expression of HLA molecules in human iPSC-MSCs under the stimulation of IFN-γ in vitro, and less inflammation and more cell survival after be transplanted into humanized mice. After the stimulation of IFN-γ, a low amount of STAT1 is phosphorylated and there is low IRF-1 and CIITA expression in human iPSC-MSCs. Next, low levels of CIITA are transcripted due to the low level of IRF-1 and the low number of p-STAT1 dimers. Consequently, there is an insufficient amount of CIITA proteins to induce the expression of HLA-II molecules. Less inflammation and more cell survival are found after the transplantation of human iPSC-MSCs into humanized mice.