Cytometry Part A

Cover image for Vol. 89 Issue 5

Edited By: Attila Tárnok

Impact Factor: 2.928

ISI Journal Citation Reports © Ranking: 2014: 28/79 (BIOCHEMICAL RESEARCH METHODS); 109/184 (Cell Biology)

Online ISSN: 1552-4930

Associated Title(s): Cytometry Part B: Clinical Cytometry

Author Guidelines

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Author Guidelines

Manuscript Submission
Editorial Policy
Software Policy
Manuscript Preparation and Submission
Standard Abbreviations for Cytometry

Cytometry Part A Supports Authors Via Submission To PubMed Central

Cytometry Part A authors whose research is funded by NIH will not have to worry about submitting their accepted manuscripts to PubMed Central (PMC). Wiley-Blackwell will support authors by posting the accepted version of articles by NIH grant-holders to PubMed Central upon acceptance by the journal. The accepted version is the version that incorporates all amendments made during peer review, but prior to the publisher’s copy-editing and typesetting. This accepted version will be made publicly available on PMC 12 months after publication. Cytometry Part A is already freely available one year following publication on Wiley Online Library. The NIH public access mandate applies to all articles based on research that has been wholly or partially funded by the NIH and that are accepted for publication on or after April 7, 2008. In addition to depositing manuscripts on behalf of NIH-funded authors, Wiley has reached an agreement with the Howard Hughes Medical Institute (HHMI) to make post-peer reviewed articles publicly available six months after final publication. HHMI will cover the cost of this service.


Cytometry Part A is a member of, and subscribes to the principles of, the Committee on Publication Ethics (COPE) (

Editorial Policy

Cytometry, Part A is the Official Journal of the International Society for Advancement of Cytometry. Clinical Cytometry is an Official Publication of the Clinical Cytometry Society.

The scope of Cytometry, Part A embraces all aspects of analytical cytology, which is defined broadly as characterization and measurement of cells and cellular constituents for biological, diagnostic, and therapeutic purposes. It includes components of cytochemistry, cytophysics, cell biology, molecular biology, physiology, pathology, image analysis, statistics, instrumentation, clinical laboratory practice, and other relevant subjects. Manuscripts may include new experimental results, descriptions of new methods or modifications of existing ones, or new interpretations of previously published data.

Manuscripts from all countries are invited but must be in English. Authors for whom English is a second language may choose to have their manuscript professionally edited before submission to improve the English. A list of independent suppliers of editing services can be found at Japanese authors can also find a list of local English improvement services at . All services are paid for and arranged by the author, and use of one of these services does not guarantee acceptance or preference for publication.

Authors are not required to be members of the International Society for Advancement of Cytometry.

Review of Manuscripts
An expert and anonymous reviewing process is important in assessing the scientific soundness of contributions to Cytometry, Part A and frequently helps authors to present their findings clearly and concisely. The Editorial Board of Cytometry, Part A selects reviewers, assists in review of manuscripts, makes recommendations concerning the suitability of manuscripts for publication, and advises the Editor-in-Chief on matters related to publication policy. All manuscripts will be evaluated independently by two reviewers. Reviewers are requested to submit written evaluations employing the following criteria:

1. Does the subject matter fall within the scope of Cytometry, Part A?
2. Does the paper describe original findings and add significantly to knowledge in its field?
3. Are the aims, results, and discussion presented clearly and concisely?
4. Are the experimental methods generally acceptable and are they described in sufficient detail to permit repetition of the work?
5. Is the documentation of the findings adequate?
6. Are the illustrations appropriate and of acceptable quality?
7. Are quantitative results statistically supported?
8. Is the list of references current and complete?

The Editor-in-Chief, on the basis of recommendations from the reviewers and the Editorial Board, may accept the manuscript, decline it, return it for revision, or send it to a third reviewer to referee differences of opinion between the initial reviewers.

Forms of Publication.
Cytometry, Part A publishes six types of contributed papers:

Articles are full-length reports of original research in all fields of analytical cytology. The investigation may contribute to the development, evaluation, or application of cytometric methods and their use in biology and medicine.

Reviews are comprehensive appraisals of current cytometric issues and advances. They are subject to the same review process as other papers. Reviews are welcomed, but authors should consult the Editor-in-Chief about the suitability of a proposed Review before its preparation.

Technical Notes briefly describe improvements or helpful modifications in procedures for cytometric and related techniques. The Technical Note should include a brief statement of purpose, an adequately detailed description of the procedure, a statement of expected results, and references to pertinent literature. Observations based on the application of the method also may be included.

Brief Reports succinctly describe biological or similar observations that would be of interest to the community but that do not contain enough information to warrant a full-length article. Methods or approaches that contain limitations are also acceptable. They should include a brief statement of purpose, an adequately detailed description of the observation(s), a statement of expected results, and references to pertinent literature.

Communications to the Editor provide a forum for communication of opinion, interpretation, and new information on scientific/political matters. Letters are judged on appropriateness of the subject and interest to the readers. They should be concise and to the point, contain 1,500-2,000 words of text, 1-2 figures or tables and a maximum 12 references. All Communications to the Editor are peer reviewed.

Length of Manuscripts and Page Charges

Authors are reminded that brevity is a virtue and contributes to the clarity of their contribution. Normally, articles will be limited to about 5,000 words and a combined total of six figures and tables. Authors will be asked to shorten excessively verbose manuscripts. Authors of articles that exceed eight printed pages will be charged $150 per excess page. Prior to publication, the Editor-in-Chief may waive this charge for authors who specifically request exemption and who provide appropriate reasons to justify their request.

Color All color figures will be reproduced in full color in the online edition of the journal at no cost to authors. Authors are requested to pay a charge of $500 per page to reproduce color figures in print. Please contact the production editor, Larry Graup at for further information.

Cytometry Part A Software Policy

On behalf of the International Society for the Advancement of Cytometry, Cytometry Part A encourages the submission of manuscripts that include the development of software for the analysis of cytometry data under the following conditions:

(1) Licensing:
The associated source code of the software described in the manuscript should, as far as possible, follow accepted community standards and be licensed under a suitable open source license (any open source initiative approved from this list is OK: Dependency on commercial software such as Mathematica and MATLAB does not preclude a manuscript from consideration, although complete open source solutions are preferred. Software must be free of any additional unreasonably restrictive licensing terms.

(2) Deposition:
It is strongly recommended that software be deposited in an open software archive (e.g. CRAN/BioConductor/GitHub). The code must be easy to locate and download without creating user accounts, logging in, or entering other personal details and must be made available during peer review in a way that preserves reviewer anonymity. Any practical issues preventing code sharing will be evaluated by the editors who reserve the right to decline the paper if important code is unavailable.

(3) Documentation:
For end-user applications, instructions for installing and using the software are prerequisite; for software libraries, instructions for using the application program interface are prerequisite.
Where feasible, results from standard test sets (e.g., from FlowCAP) should be included. Where possible, test data should not have any dependencies (e.g., for example, a database dump). Cytometry Part A supports for the deposition of FCS data files. Parameter settings for test data must be included in a way that allows readers to repeat the published results. Descriptions of availability and licensing and documentation should be included in an ‘Availability and Requirements’ section.

Cytometry Part A editors and editorial board members reserve the right to request additional assets from authors to ensure a reasonable level of compliance with the above Software Policy. In extreme cases, based on the judgment of reviewers and editors, Cytometry Part A may delay transition of a manuscript to subsequent phases in the standard editorial workflow until the Software Policy requirements are adequately satisfied.

Manuscript Submission

Authors are strongly encouraged to submit their manuscripts online at .

Authors are required to include the names and addresses of two persons outside their own institutions who might be qualified to serve as reviewers. Authors can request their manuscript not be reviewed by reviewers whose opinion, they believe, may be unfavorably biased. Authors may also request their manuscript be transmitted to specific members of the Editorial Board.

Authors are required to submit a brief statement including the important contributions of the submitted paper and the reason(s) it should be published in Cytometry, Part A.

Copyright . Submission of a manuscript implies that it has been approved by all the named authors, that it reports unpublished work, and that it is not under consideration for publication elsewhere. If the manuscript is accepted for publication, it will become the sole property of the publisher and will be copyrighted in the name of the publisher. The manuscript, in whole or part, must not be published elsewhere in either English or another language.

Is the Minimum Information about a Flow Cytometry Experiment (MIFlowCyt) standard met? MIFlowCyt states the minimum information required to report flow cytometry (FCM) experiments to allow for future independent validation and interpretation of experimental data. For more information and a summary of the required information, please see the article, "MIFlowCyt: The Minimum Information About a Flow Cytometry Experiment" by Jamie A. Lee et al.

Conflict of Interest Disclosure
Cytometry Part A requires that all authors disclose any potential sources of conflict of interest. Any interest or relationship, financial or otherwise, that might be perceived as influencing an author’s objectivity is considered a potential source of conflict of interest. These must be disclosed when directly relevant or indirectly related to the work that the authors describe in their manuscript. Potential sources of conflict of interest include but are not limited to patent or stock ownership, membership of a company board of directors, membership of an advisory board or committee for a company, and consultancy for or receipt of speaker’s fees from a company. The existence of a conflict of interest does not preclude publication in this journal.

If the authors have no conflict of interest to declare, they must also state this at submission.It is the responsibility of the corresponding author to review this policy with all authors and to collectively list in the cover letter to the Editor-in-Chief, in the manuscript (under the Acknowledgments section), and in the online submission system ALL pertinent commercial and other relationships.

Manuscript Preparation

Manuscript Organization

All articles should be double-spaced throughout, with each page numbered consecutively. Begin each section, described below, on a new page.

Title Page:
The opening page must contain: a) complete title (be succinct and informative, avoid subtitles); b) name and affiliations of each author (specific to the department level); c) a running headline, not to exceed 45 characters in length, including spaces; d) complete contact information -- the corresponding author's mailing address, phone and fax numbers and e-mail address; e) credits for research support as a footnote to the title; and f) any important miscellaneous information, e.g., that the research is based on a conference presentation, also as a footnote to the title.

Abstract and Key terms:
Authors are required to provide an abstract. The abstract should describe the reason for the study, how the study was performed, the most important results, and what is concluded from the results. Below the abstract, provide three to ten key words or short phrases for indexing. The subject under investigation and technologies or methods employed must be included. The Medical Subject Headings List from Index Medicus should be used whenever possible.

State the aim of the work and the problem that stimulated it. Briefly summarize relevant published investigations.

Materials and Methods:
Present in sufficient detail to permit the work to be repeated by other investigators.

Manuscripts that contain flow cytometry data should comply with the MIFlowCyt standard and should include the following:

Experiment overview
Flow sample and specimen description
Instrument details
Data analysis details
Checklist for compliance

Detailed descriptions of each of the items listed above and examples of compliance are given in the following publications: click here

Present results concisely, avoiding redundant use of both tables and figures to illustrate the same data set.

Limit to interpretation of results, with a minimum of recapitulation of findings.

Cite references in text and list at the end of the article. Follow the instructions for Literature Cited, below.

Figures, Tables, and their Legends:
Illustrations, tables and legends must be numbered consecutively and cited sequentially in text; design these to be self-explanatory without reference to the text. We do not accept Figures or Tables embedded within the text of the manuscript. Please include these elements, either at the end of your manuscript or as separate files.
Rapid Inspector TM gives authors of scientific, technical, and medical journals a resource for certifying their illustrations prior to submission. Utilizing this software will ensure that your graphics are suitable for print production. To download this journal's free Rapid Inspector software, please visit

Authors are encouraged to visit for supported artwork formats.

General Rules and Style

Spelling: Cytometry, Part A uses Webster's New Collegiate Dictionary as its standard for spelling.

Style: For information on style, abbreviations, nomenclature, and the preparation of copy, authors are referred to the Council of Biology Editors (CBE) Style Manual 6th edition, available from the Council of Biology Editors, Inc., 11 South La Salle Street, Chicago, IL 60603-1210. Abbreviations and acronyms are spelled out at their first use and abbreviated in parentheses immediately thereafter.

Units: The units for all Measurements must conform to the International System of Units (SI). If a measurement is made in other units, then the SI equivalent should be given in parentheses.

Products and Companies: If authors refer to specific products, they must identify the company by citing, in parentheses, the company's name, city, and state or country.

Literature Cited:

References may be made only to published works and papers in press. Work in progress, unpublished experiments, and personal communications are specifically excluded from the reference list but may be acknowledged in parentheses in the text. Citation of references in text must use the Citation-Sequence format in which the reference number is entered in parentheses.

References must be listed sequentially at the end of the maunscript. Abbreviations of journal names must conform to those used in Index Medicus . Authors are responsible for the accuracy of the references. The following formats must be used:

Journal Article
1. Hüller R, Glossner E, Schuab S, Weingärtner J, Kachel V. The macro flow planktometer: a new device for volume and fluorescence analysis in macro plankton including triggered video imaging in flow. Cytometry 1994;17:109-118.

Article in a book or comparable publication

2. Gary JW, Cram LS. Flow karyotyping and chromosome sorting. In: Melamed MR, Lindmo T, Medelsohn ML, editors. Flow cytometry and sorting. 2nd ed. New York: John Wiley & Sons Inc.; 1990. p 503-529.


3. Givan AL. Flow cytometry: first principles. New York: John Wiley & Sons Inc.;1993. 1223 p.


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In addition to publication online via Wiley Online Library, authors of OnlineOpen articles are permitted to post the final, published PDF of their article on a website, institutional repository, or other free public server, immediately on publication.

Any authors wishing to send their paper OnlineOpen will be required to complete the payment form available from our website at:

The author’s fee for OnlineOpen is $3,000 US. OnlineOpen will be activated upon payment and can be ordered at any point prior to, or after, acceptance.

Prior to acceptance there is no requirement to inform an Editorial Office that you intend to publish your paper OnlineOpen if you do not wish to. All OnlineOpen articles are treated in the same way as any other article. They go through the journal's standard peer-review process and will be accepted or rejected based on their own merit.

Editorial Office
Requests for copies of the Editorial Policy and Instructions to Contributors, requests for clarification of specific issues, manuscripts submitted for publication, and all other communications with the Editorial Office should be addressed to the Editor-in-Chief:

Attila Tàrnok, Ph.D.
Universität Leipzig
Klinik für Kinderkardiologie
Strümpellstrasse 39
04289 Leipzig
Telephone: +49-(0)341-8 65 24 30
Fax: +49-(0)341-8 65 11 43

European contributors may address their requests to the European Editor:

Janos Szollosi, Ph.D.
Department of Biophysics and Cell Biology
Medical University,
School of Debreceen,
P.O. Box 39
Nagyer dei krt, 98
Debreceen, Hungary, H-4012

Bioimaging contributors may address their requests to the Bioimaging Editor:

Stephen Lockett
Boyle St., Bldg. 1050
Frederick, MD 21702
Telephone: 301-846-5515

Reprints may be purchased at Wiley is pleased to extend a 50% discount on reprint orders to ISAC and CCS members.

Guidelines for Electronic Submission


Software and format . Microsoft Word .doc or.docx files are preferred, although manuscripts prepared with any other microcomputer word processor are acceptable. Refrain from complex formatting; the Publisher will style your manuscript according to the Journal design specifications. Do not use desktop publishing software such as Aldus PageMaker or Quark XPress. If you prepared your manuscript with one of these programs, export the text to a word processing format. Please make sure your word processing program's "fast save" feature is turned off. Please do not deliver files that contain hidden text: for example, do not use your word processor's automated features to create footnotes or reference lists.

File names . Submit the text and tables of each manuscript as a single file. Name each file with your last name (up to eight letters). Text files should be given the three-letter extension that identifies the file format. Macintosh users should maintain the MS-DOS "eight dot three" file-naming convention.

All print reproduction requires files for full color images to be in a CMYK color space (not RGB). If possible, ICC or ColorSync profiles of your output device should accompany all digital image submissions.

Software and format . All illustration files should be in TIFF or EPS (with preview) formats. Do not submit native application formats.

Resolution . Journal quality reproduction will require greyscale and color files at resolutions yielding approximately 300 ppi. Bitmapped line art should be submitted at resolutions yielding 600-1200 ppi. These resolutions refer to the output size of the file; if you anticipate that your images will be enlarged or reduced, resolutions should be adjusted accordingly.

File names . Illustration files should be given the 2- or 3-letter extension that identifies the file format used (i.e., .tif, .eps).

Standard Abbreviations for Cytometry




aminoactinomycin D

arbitrary unit


antibody binding capacity

alternating current

analog to digital converter


acquired immune deficiency syndrome

acute lymphoblastic leukemia

7-amino-4-methylcoumarin-3-acetic acid

acute myeloblastic leukemia

artificial neural network


cytosine arbinoside

adenosine triphosphate: ADP, AMP, GDP, GMP, GTP, CTP, UDP


base pair


bovine serum albumin

cluster of differentiation

cyclin dependent kinases

complementary DNA

comparative genomic hybridization

chronic lymphocytic leukemia

chronic myeloid cytic leukemia


concanavalin A

counts per minute

colony-stimulating factor

coefficient of variation

3,3 diaminobenzidin

4,6-diamidino-2-phenylindole, dihydrochloride

dendritic cell

direct current

Dulbecco's minimum essential medium


deoxyribonucleic acid



double stranded

ethidium bromide

Epstein-Barr virus

ethylene-diaminetetraacetic acid

enzyme-linked immunosorbent assay

electron microscopy

endoplasmatic reticulum

expressed sequence tag

fluorescence-activated cell sorting

fetal calf (bovine) serum

flow cytometry

fluorescein diacetate

fluorescence in situ hybridization

fluorescein isothiocyanate

fluorescence resonance energy transfer

forward angle scattered light: FLS

full width at half maximum


granulocyte colony-stimulating factor

green fluorescent protein

granulocyte-macrophage colony-stimulating factor



fetal hemoglobin

Hank's balanced salt solution

N-2-hydroxyethelpiperazine-N2 ethansulphonic acid

human immunodeficiency virus

human leukocyte antigen

high performance liquid chromatography

human serum albumin





isoelectric focusing




Iscove's modified Dulbecco's medium

international unit



light emitting diode


monoclonal antibody

macrophage CSF

multidrug resistance

molecules of equivalent soluble fluorochrome

mean fluorescence intensity

major histocompatibility complex

mononuclear cell

messenger ribonucleic acid

molecular weight

n or No

cell natural killer cell

not significant

optical density


polyacrylamide gel electrophoresis

peripheral blood lymphocyte

peripheral blood mononuclear cells

phosphate-buffered saline

polymerase chain reaction


polyethylene glycol

peridinin chlorophyll protein


propidium iodide

protein kinase C

polymorphonuclear leukocyte


pokeweed mitogen

random access memory

retinoblastoma protein

red blood cells

restriction fragment length polymorphism